Source: CORNELL UNIVERSITY submitted to NRP
INFORMATION-BASED ECOLOGICAL MANAGEMENT OF POTATO/TOMATO LATE BLIGHT
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1007538
Grant No.
2015-70006-24274
Cumulative Award Amt.
$300,000.00
Proposal No.
2015-06498
Multistate No.
(N/A)
Project Start Date
Sep 1, 2015
Project End Date
Aug 31, 2018
Grant Year
2015
Program Code
[ARDP]- Applied Research and Development Program
Recipient Organization
CORNELL UNIVERSITY
(N/A)
ITHACA,NY 14853
Performing Department
Plant Pathology
Non Technical Summary
This is an extension-led project that requests $300,000 for two years to improve the efficacy of late blight management in a more ecologically-friendly manner than has been standard practice. It involves persons from Florida, Maryland, New York, North Carolina, Pennsylvania, Washington, and Wisconsin. Each co-PI will provide information obtained locally and communicate that information to the rest via a website that is publicly available. Genotypic analyses will determine the pathogen strain (or clonal lineage) and importantly will also enable the early identification of new strains of the pathogen. The information obtained by the project will enable precision management (management directed against the specific pathogen causing the disease). Thus, the project will enhance environmental friendliness of late blight management, and it will improve late blight suppression.
Animal Health Component
80%
Research Effort Categories
Basic
20%
Applied
80%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
21601991160100%
Knowledge Area
216 - Integrated Pest Management Systems;

Subject Of Investigation
0199 - Soil and land, general;

Field Of Science
1160 - Pathology;
Goals / Objectives
Our overall goal is to enhance the effectiveness of potato and tomato late blight management while at the same time improving the efficiency of late blight management. The project will reduce the impact of pesticide in the environment and also reduce costs to growers. We will achieve the overall goal through a series of extension-led and research-led objectives that develop the information needed for "precision IPM":Extension-led objective i) identify and rapidly report locations of late blight outbreaks in FL, MD, NY, NC, PA, WA and WI. Use sentinel plots to identify strains that overcome commercially available host resistance.Extension-led objective ii) determine the genotype of the late blight strains causing these outbreaks and inform growers of the epidemiologically important traits of these strains. Research-led objective i) determine the phenotypic characteristics of any "new" strains of P. infestans in these outbreaks.
Project Methods
Approaches and Procedures Extension-led objective i) identify and rapidly report locations of late blight outbreaks in FL, MD, NY, NC, PA, WA and WI.Funding from this project will enable each co-PI/ to coordinate a monitoring activity in her/his region. Additionally, each is associated with a network of informed personnel in her/his state. These personnel are employees, colleagues in cooperative extension, the public at large, a grower community or the private sector advisors in her/his state. These personnel will monitor production fields and inform the co-PI of potential late blight occurrences in the state. Each co-PI will confirm the diagnosis and will ensure that samples from late blight outbreaks are submitted for genotypic analysis. This may be through direct sample submission of plant samples or submission of lesions squashed onto FTA cards (all submissions will be sent to the Fry lab). Results of genotypic analysis will be provided to the submitter in 24-48 hours and this information will be relayed (by the co-PI) to the grower, USAblight.org website, other growers in the region (without revealing the exact location of outbreak), educators and any other interested parties. Frequent regional updates will be provided to all stakeholders not only through USAblight.org, but also via newsletters, twilight meetings, blogs, and other available resources. Updates will be made to the USAblight.org website to keep content current and informative including; outbreak information, disease identification and management strategies, control options and new videos. This USAblight.org website is hosted at NCSU and maintenance of the site is supported by this project (see budget). Importantly, other scientists, educators, growers and home owners are also encouraged to submit samples from late blight outbreaks.Because surveillance of the P. infestans population is critical for proper disease management, each co-PI will also plant a sentinel plot including susceptible and currently available resistant varieties of tomato or potato. These plots will enable the rapid detection of isolates that can overcome host resistance and leaf tissue containing these isolates would be immediately send to the Fry lab for genotypic and phenotypic analyses.Extension-led objective ii) determine the genotype of the late blight strains causing these outbreaks and inform growers of the epidemiologically important traits of these strains. a) The samples submitted to the Fry lab will be assayed using microsatellite markers as described in Lees et al. (2006) and as subsequently modified (see Danies et al 2014). The assays are typically completed within 24-48 hr, and the results will be immediately transmitted to the sender (and co-PI if the sender is not a co-PI). The co-PI will upload the information to the USABlight website and will also inform the grower/manager. Thus the website will be kept up to date by each co-PI. Alerts will be sent to a large number of users as result of each update. The transmission of information to the grower will contain an interpretation of the important epidemiological implications of the type of strain detected in the outbreak. (Important epidemiological traits are those listed in Table 1.) We will record the number of samples received, and the identity of the genotype(s) in each sample.b) Each author will inform the grower community in his/her state/region of the results of the sampling and the implications of these results. The vehicles for communication include grower email alerts, internet postings, newsletters, and diverse types of meetings.Research-led project i) determine the phenotypic characteristics of any "new" strains of P. infestans in these outbreaks.Since 2009, most strains of P. infestans have belonged to one of five major clonal lineages (US8, US11, US22, US23, US24), and the phenotypic characteristics of these strains have been determined and published (Danies et al. 2013a) (Saville et al. 2015). These phenotypic characteristics have been used by growers in selecting the most effective fungicide. For example, if US11 is causing the disease, fungicides with mefenoxam are not used.However, some strains have not been in one of these clonal lineages. An example is a strain (interim name is 2014 "B") detected in western NY in 2014. Upon conclusion of the season, phenotypic analyses revealed that this strain was pathogenic on both potato and tomato and was intermediate in its resistance to mefenoxam (unpublished data). New strains detected in 2015/2016 or 2016/2017 will be similarly investigated. New strains will be isolated into pure culture and then assessed for mating type, mefenoxam sensitivity and for potato/tomato pathogenicity as described in Danies et al (2013). The relative aggressiveness of these new strains will be predicted by using these data in a complex mechanistic model of the late blight disease (Andrade-Piedra et al. 2005) as illustrated in the study by Kato et al (Kato et al. 1997).Management of the project. The co-PI and PIs have a history of collaborating in the AFRI CAP project. Funds in that project enabled the development of the website and enhancements in the Decision Support System. Each author is experienced in sampling and updating the website. Each author is also very experienced in communicating results such as will be identified in the proposed project. Communications during the year will occur via email, and there will be planning and debriefing conference calls before and after each season (spring and fall). Much communication will also occur via the USABlight website. Any issues concerning this site will be addressed at NCSU under the direction of co-PI Ristaino. Genotyping will be done at Cornell under the direction of PI Fry. The phenotypes of any new genotypes will be investigated at Cornell.

Progress 09/01/15 to 08/31/18

Outputs
Target Audience:The target audience included professional integrated pest management personnel, potato and tomato growers, and extension personnel. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?During the several years of the project, the project partially supported several undergraduates and one graduate student. How have the results been disseminated to communities of interest?The project has reached thousands of IPM personnel, growers, field staff, extension personnel, and many home gardeners in the USA. The website was visited approximately 70,000 times over the course of the three years -- including persons from all 50 states. In the USA, participants have reached thousands of interested persons via, meetings, email and newsletters. In addition to the audience in the USA, persons from more than 125 countries have visited the website. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Over the course of the several years of the project, the goals were met. The understanding of the target audience was improved with up-to-date information about the status of late blight in the USA. In each of the first two years there were many meetings, presentations and newsletters by participants. In each of the three years, there were rapid reports of late blight occurrences in the USA, and the genotypes causing the outbreaks was reported. The project was primarily a two-year project with most activity occuring in FY 2016 and 2017, and reports for 2016 and 2017 have been submitted previously. There were 69 genotype assessments in 2016, 48 in 2017 and 30 in 2018. US23 was the dominant genotype in each year, with a few US8 being detected. Late blight in 2018 was somewhat reduced compared to previous years, with more than 40 observations of late blight reported on USABlight. In 2018 a new genotype (US25) was detected and described. US 25 was determined to be A2 mating type, resistant to mefenoxam, and pathogenic on both potatoes and tomatoes. Of the 30 samples received by the Fry lab in 2018, most were US23, with a couple US8 and a couple US25. We sent out more than 2800 alerts during the 2018 season. Although the project was planned mainly for two years, some investigators also planted sentinel plots in 2018. There were no examples of resistance-breaking isolates of P. infestans. During the course of the project, no strains of P. infestans that could overcome Ph2 and Ph3 were detected anywhere in the 8 locations participating in the project. These genes have been incorporated into several commercially available tomato cultivars. Participants in the project continued to give talks to growers and to extension personnel. For example in the final year, participants in NY, WA, FL and PA gave talks and communicated with their IPM and grower clientele via meetings and email. Over the course of the 2+ years of the project, participants have presented up-to-date information about late blight in more than 70 meetings. Many participants also provided weekly late blight updates through diverse media including: a toll-free telephone line, e-mail delivery system during the growing season (1 April - 31 August), phone contacts and weekly newsletters. During the course of each growing season, the recipients of weekly information totaled several thousand each week. Because of the success of the project and the rapid turnaround of information (late blight location and genotype causing the outbreak), the participants are searching for mechanisms to continue the project. The clientele has been particularly interested in knowing the genotype of the strain that is causing late blight near them.

Publications


    Progress 09/01/15 to 08/31/16

    Outputs
    Target Audience:The Target audience is professional integrated pest management personnel, potato and tomato growers, and extension staff. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The cooperators on the project combined to make 31 presentations to diverse groups of stakeholders. Each of these presentations was an educational meeting with growers, fieldmen, and extension staff in attendance. In many of the meetings, postdocs and students were in attendance. How have the results been disseminated to communities of interest?In each of the 31 presentations, results from the current situation were disseminated. Additionally, each of the visitors to the website had access to the current information. What do you plan to do during the next reporting period to accomplish the goals?We will continue the activities that we proposed in our application. Information obtained during the season, will be communicated to stakeholders on a near real-time basis via the website. We will continue to bring the most current data to our stakeholders via many talks presented by the co-PIs.

    Impacts
    What was accomplished under these goals? Late blight was much reduced in severity in 2016 compared to many previous years. The extreme dryness in much of the eastern USA was probably the most important contributing factor. Our project certainly has had some effect in minimizing the effect of some of those outbreaks. Some of these had significant impact. These occurrences were logged into the USABlight system. Thus, growers, field managers and extension personnel had current information all season long. Late blight did not occur on any of the sentinel plots conducted collaborators. Sixty-nine samples were submitted to the Fry Lab for genotypic and phenotypic assessment. In chronological order in 2016, these samples came from Florida, California, Maryland, Missouri, Washington, Manitoba Canada, Maine, North Carolina, Colorado, and Virginia. Most samples were of the US23 clonal lineage. Most individuals of this lineage have been sensitive to mefenoxam, but one sample from Maryland was resistant. This was a surprise, and was discovered because of the effort sponsored by this project. This information was conveyed to the grower and to the entire community. Reports of late blight are listed on the USABlight website. The website was visited many times In 2016. There were 21,493 site visits from 15,760 unique visitors. The most visited daywas Tues, August 2nd(166 visits). Here are other facts about the traffic on the website. User Information 141 Countries. Top 5: United States (60.4% of all visits), India (12.1%), Canada (4.9%),UK (2.6%), Kenya (2.2%) All 50 US States. Top 10: NY (13.3% of all visits), PA (9.2%), CA (5.6%), NC (5.6%), MA (5.6%), MI (4.5%), WI (4.2%), IL (3.1%), FL (3.0%), TX (3.0%) Content Top 5 Visited Pages: Homepage (27.4% of all visits), Map (20.0%), Symptoms on Tomato (9.6%), Symptoms on Potato (8.1%),What is Late Blight? (8.0%) Alerts 465 Active alert sites (as of October 25th, 2016) 32 sites created between Oct 1 2015 to Sept 30, 2016: 1168 Alerts sent to 281 unique emails/texts between Oct 1 2015 to Sept 30, 2016:

    Publications