Progress 09/01/15 to 08/31/18
Outputs
Target Audience:This funded proposal is professional development project. The main goral is for Project Director to learn basic techniques and bioinformatics behind Genome-Wide Association Mapping. Although the project did not target any audiences but PI himself, the information learnt is directly benefit to the underrepresented students at the Virginia State University.In fact, the projectcurrently running in the lab is using the learnt technology and 2 students iare under PI's supervising. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project provided the PI the opportunity to be trained on GWAS analysis. Through this training, the PI is able to be broaden his research methodiology in dissecting agronomically important traits. To practice these learnt technologies, the PI's new Evans Allen project is designed to map the shoot-rooting ability trait in tomato. In addition, the PI will also train undergraduate students with such technoques. How have the results been disseminated to communities of interest?The project is focusing on PI's training in new techniquies,therefore, thetr are no research results to be disseminated. However, suchlearnt techniques areused to train our undergraduate students througheveryday's research activities at VSU. Techniques learnt and data collected from this project, together with continued efforts on this research topic,will be used as preliminary data to seek additional support for soybean GWAS study in the future. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
The project is designed to use research -based strategy to allow PI being trained on GWAS related techniques and analysis. During the funding period, the PI introduced 296 lines of soybean mapping population from USDA collaborator and tested their drought stress abiliy. In additon, we also identified several lines with interesting agronomic traits such as pot numbers andmaturity status and integrated them into our soybean breeding program. During the funding pweriod, the PI also traveled to USDA Research Center at Beltisville for several time to learn genotyping method of GWAS and its bioinformatic analysis precedures. Through this project, the PI established collaboration between VSU and USDA laboratory. Such collaboration will benefit future genetic studies at VSU, not only for soybean relateed research, but also for other basic research related to gene mapping and gene cloning.The PI is currentlyapplying some of the techniques for his Evans Allen project.
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Progress 09/01/16 to 08/31/17
Outputs
Target Audience:This is professional development grant. The main goal of this project is to train the PI on GWAS analysis and to establish collaboration between USDA and VSU. However, in a long run, this project can have abroader audience including scientific communities as well as US farmers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project provided the PI the opportunitiy to be trained on GWAS analysis.The PI was able tocan broaden his research methodiology in dissecting agrinomically important traits.The PI's new Evans Allen project is involves mapping of the rooting ability trait from tomato. He used the technique he learned from this project for his Evans Allen Research. How have the results been disseminated to communities of interest?This focus of the project is trainingnew technoilogies, therefore tit is not necessary to disseminate the final result to the communities. However, the PI used the techniquest he learned to train students.. What do you plan to do during the next reporting period to accomplish the goals?Thie project will be completed at 08/31/2018. We will use this last grant period to collect some phenotypic traits of soybean drought tolerance in a segregation population. The generated and real data will be used for GWAS analysis. .
Impacts
What was accomplished under these goals?
During this grant period, the PI visited USDA Research Center at Beltisville, Maryland. Discussed with Dr. Qijian Song about genotyping method of GWAS and bioinformatic analysis precedure of GWAS. The PI learnt the techniques of GWAS and the software used to analyzeGWAS data.
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Progress 09/01/15 to 08/31/16
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The PI was able to establish collaboration between VSU and USDA scientist for the future research. Short-term visit of USDA laboratory also provided PI the opportunity for learn the concept of GWAS. Further technical practice will be held in the summer 2017, if arrangement of lab maintenance is allowed for PI's short sabbitical leave without affecting laboratory research. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?Professional training will be continued for PI to learn GWAS analysis. Greenhouse experiments and feild test will be performed in the growing season of 2017.
Impacts
What was accomplished under these goals?
This project was designed to use research-based strategy to allow the PI being trained on GWAS related techniques and analysis. During the first year of the project, we mainsly focused the seed increase in the field. We successfully introduced 296 lines of a mapping population from our USDA collaborator and grew in our research farm. Seeds for all soybean lines were harvested manually before December, 2016, and hand-cleaned. The PI also visited the USDA collaborator lab to discuss the project and familiar with the equipment used for GWAS genotyping.
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