Source: UNIV OF HAWAII submitted to NRP
EVALUATION OF ESSENTIAL AND NEURAL TOXIN TRACE MINERALS IN COMMONLY CONSUMED FOODS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1007047
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2015
Project End Date
Sep 30, 2019
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIV OF HAWAII
3190 MAILE WAY
HONOLULU,HI 96822
Performing Department
Human Nutrition, Food & Animal Sciences
Non Technical Summary
Since the late 70's, there has been a rapid increase in neural developmental problems with autism spectrum disorder affecting approximately 1 in 68 children born in the U.S. today (approximately 3,000 children in Hawaii). Recent research shows that a low iron intake before and during pregnancy is associated with a greater risk of having an autistic child. Both iron and zinc play critical roles in normal brain development, whereas other minerals, such as aluminum, manganese and arsenic have been shown to be neural toxins when consumed in excess.During the period between 1970 to present, the typical food make-up of the American diet has changed considerably. Key foods containing both iron and zinc have decreased (heme-iron containing foods) and foods containing iron and zinc inhibitors have increased (e.g. calcium fortified foods, foods high in tannins and polyphenols). Notably, foods that contain aluminum, manganese, and arsenic have also increased considerably. Additionally, environmental exposure to aluminum has increased with common use in fertilizers; manganese has replaced lead in both paint and gasoline; and aluminum and/or arsenic is commonly found in poultry litter used in caged pens. The foods analyzed will reflect changes in the American diet and will be based on the Total Diet Study, NHANES Studies (1976-2012) and focus groups with mothers of autistic children.
Animal Health Component
30%
Research Effort Categories
Basic
30%
Applied
30%
Developmental
40%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
7015010101080%
7025010101010%
7245010101010%
Goals / Objectives
To determine if common U.S. foods consumed by toddlers and woman of reproductive age exceed Provisional Tolerable Weekly Intake (PTWI) for aluminum, manganese, arsenic and other problematic minerals that are either neurotoxins or potentially contribute to low iron and/or zinc status .Objective 1- Determine availability of analytical mineral data for foods commonly consumed in the US Mainland and Hawaii.Objective 2- Create literature mineral database.Objective 3- Chemically analyze commonly consumed foods with inadequate recent analytical data.Procedure: Foods chosen will reflect both the most commonly consumed foods as reflected by the Total Diet Study and by food records from NHANES (1976 to 2010) datasets. Since these do not represent foods in Hawaii, supermarket managers in Hawaii will be contacted for their input into commonly consumed foods.Objective 4- Identify potential neurotoxin and iron/zinc inhibitors in food.Procedure: Using NHANES diet records (1976-2014), we will determine if published and our analytical neurotoxin minerals would exceed PTWI safe levels recommended by USDA and/or FAO/WHO or the Upper Limit set by Institute of Medicine. This will be done by estimating the quantity of problem minerals/kg body weight based on individual's diet records. The potential for decreased bioavailability of iron or zinc will also be evaluated based on proportions of inhibitory minerals.
Project Methods
Methods are listed along with the specific objectives.Objective 1- Determine availability of analytical mineral data for foods commonly consumed in the US mainland and Hawaii.Procedure: Review literature for diet and supplement information relating to trace mineral content. Research will include: information using Pubmed, Web of Science, Quertle, Google, Google Scholar, as well as conference proceedings.Review data from the F.D.A. Total Diet Study and USDA Nutrient Database Release 27 / 28 data for analytical mineral data (http://www.fda.gov/Food/FoodScienceResearch/TotalDietStudy/default.htm and http://ndb.nal.usda.gov/ndb/search/list)Objective 2- Create literature mineral databaseProcedure: Compile mineral data collected from literature into a JMP mineral databaseObjective 3- Chemically analyze commonly consumed foods with inadequate recent analytical data.Procedure: Foods chosen will reflect both the most commonly consumed foods as reflected by the Total Diet Study and by food records from NHANES (1976 to 2010) datasets. Since these do not represent foods in Hawaii, supermarket managers in Hawaii will be contacted for their input into commonly consumed foods. Timeline forfoods explored for Objective 3include:Yr 1-Grain Products;Yr 2 -Fruits and Vegetables; Yr 3-4 Other Foods.Foods identified as commonly consumed but with inadequate analytical data will be purchased and chemically analyzed for mineral content using AOAC procedures. Prepared samples will be sent Louisiana Agricultural Experiment Station soil lab and the following elements will be determined - AgRoutine Metals Package (P, K, Ca, Mg, S, B, Zn, Cu, Fe, Mn, Mo, Al, and Na) and their Environmental Metals (Pb, Cd, As, Ni, Cr, Se, Co, and Si) and N.YEARS 1-4Objective 4- Identify potential neurotoxin and iron/zinc inhibitors in food.Procedure: Using NHANES diet records (1976-2014), we will determine if published and our analytical neurotoxin minerals would exceed PTWI safe levels recommended by USDA and/or FAO/WHO or the Upper Limit set by Institute of Medicine. This will be done by estimating the quantity of problem minerals/kg body weight based on individual's diet records. The potential for decreased bioavailability of iron or zinc will also be evaluated based on proportions of inhibitory minerals. Timeline forfoods explored for Objective 4include:Yr 1-Grain Products;Yr 2 -Fruits and Vegetables;Yr 3-4Other Foods.

Progress 10/01/17 to 09/30/18

Outputs
Target Audience:Target audiences were primarily focused on females of reproductive age or those who influence females of reproductive age. These efforts included both undergraduate and graduate university students in three courses, as well as females seen for nutrition counseling. Two presentations were made to college and university health professionals and one graduate student poster presentation at the College of Tropical Agriculture and Human Resources Student Symposium reached students and faculty alike. Other Outreach activities included community talks to retirees and YWCA groups. Changes/Problems:The National Health and Nutrition Examination Survey (NHANES) does not contain data for Mn nor the other heavy metals more frequently found in the 7500+foods. Due to the increasing research studies reporting associations between manganese exposure and neurologic symptoms in infants and children, there is a growing need for Mn data. Without adequate Mn information in the food data used by NHANES, potential causes of health issues may too easily be overlooked. This oversight may delay corrective actions needed in the prevention of developmental problem related to the autism spectrum. What opportunities for training and professional development has the project provided?This project trained two graduate students in basic food composition lab analyses and database evaluation. It helped to train these students in using data mining software and in overall practical presentation of results. How have the results been disseminated to communities of interest?Outreach was accomplished through handouts and presentations to students at the University of Hawaii at Manoa, Pacific Coast College Health Association professionals and one-on-one individual counseling at the University Health Services at Manoa. What do you plan to do during the next reporting period to accomplish the goals?We plan to complete the following: 1. Chemical analyses of most commonly consumed high manganese foods; 2. Make available an updated Mn database, based on literature and analyses; 3. To evaluate potential Mn intake of the NHANES population, we will input Mn data for NHANES food codes and run model analyses; 4. Publish results both in scientific journals but also make this information available via popular media.

Impacts
What was accomplished under these goals? The trace element manganese is both an essential nutrient and a potential neurotoxin when consumed in excess over time. Evaluation of manganese (Mn) data from both lab analyses and international food databases indicates that high Mn foods are almost exclusively plant-based, with many containing 10-50 times the Mn found in foods from animal sources. The National Health and Nutrition Examination Survey (NHANES) is designed to continuously/repeatedly assess the health and nutritional status of adults in America. The NHANES dataset, however, does not include Mn as part of its nutrient database. Therefore, to better understand how high Mn foods are presented to the public, we explored the potential influence of the increasingly popular Internet marketing term of "Superfood." This term seems to be generically used to assist individuals in choosing healthier food options. Our study looked at which foods would be found in a common search and the relative frequency that a consumer would see any particular "Superfood" on Internet websites. On a single day, the Google search engine was used to search the term "superfood list." Data were obtained from the top 50 sites. Foods were then categorized into modified USDA and culinary food groups. A list consisting of 1215 "superfoods" was downloaded representing 385 unique foods. Sites recommended from 0 to 127 foods. Plant sources represented more than 90% of the unique foods, animal sources represented about 9%. Regardless of the Internet site focus or author credentials, Internet sites significantly promoted higher Mn foods within food groups. The results of this study indicate that those making food choices based on the "superfood" concept may be unknowingly consuming excess Mn and potentially adversely affecting long-term health. Adding Mn to the database used to evaluate What We Eat in America (WWEIA) would provide a better evaluation of potential Mn risks to the overall public.

Publications

  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Titchenal, C.A. and Dobbs, J. 2017. Plant-based Diets  Potential Strengths, Weaknesses and Toxicities. 2017 Pacific Coast College Health Association Annual Meeting. Striking Gold, Mastering Emerging Trends in College Health, October 11-14, 2017, Reno, Nevada.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Dobbs, J. and Titchenal, C.A. 2017. Growing Campus Food Insecurity Has Substantial Physical and Mental Health Implications. 2017 Pacific Coast College Health Association Annual Meeting. Striking Gold, Mastering Emerging Trends in College Health, October 11-14, 2017, Reno, Nevada.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Valdez, D.K., Titchenal, C.A., and Dobbs, J. 2018. Potential of Internet Influence on Superfood Choices. 2018 CTAHR-COE Student Research Symposium. University of Hawaii. Honolulu, Hawaii.


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:Target audiences reached this year include university classroom instruction and outreach activities including newspaper audience, Hawaii State Farm Fair visitors (families), Outreach community talks to retirees and YWCA groups. The Honolulu Star-Advertiser is Hawaii's major newspaper and is ranked 12th out of the 15 largest daily newspapers in the United States. The combined daily print and online digital circulation exceed 152,000 copies. Additionally, nutrition researchers and health professionals were reached via one oral presentation and one graduate student poster presentation at the 2016 Hawaii Public Health Conference (Oct. 13). Changes/Problems:Databases from around the world were combined into a JMP data mining application. A memory chip malfunction required that the data be re-entered to assure data integrity. This issue delayed the identification and chemical analyses of foods that will now be done in 2018. Michael Dunn (co-PI) retired, but has continued to be part of the discussion regarding potentially toxic levels of dietary manganese. What opportunities for training and professional development has the project provided?This project trained one graduate and one undergraduate student in the many challenges related to working with multicultural food composition databases and data mining software applications. How have the results been disseminated to communities of interest?Outreach vehicles included Hawaii State Farm Fair demonstrations and handouts, the University of Hawaii at Manoa Health Services individual counseling, presentations for health professionals at the Hawaii Public Health Conference, community presentations, and publication of Honolulu Star-Advertiser newspaper articles. What do you plan to do during the next reporting period to accomplish the goals?We plan to continue adding published manganese data to our database, as well as chemically analyzed manganese data from commonly consumed local cultural foods. The database will be made available to other researchers through published data and in excel file format.

Impacts
What was accomplished under these goals? Fifty food composition databases were examined for manganese data. Thirty percent of these databases listed manganese but often only for a small subset of foods. Many national databases glean data from other published databases, including the USDA National Nutrient Database for Standard Reference. Additional data were also collected to determine the percent of food values that were based on one or two food samples or were imputed. Where this information was available, data indicated that most data represents only a few chemically analyzed samples and may not be representative of individual foods. Sample diets of commonly consumed high manganese foods also were evaluated regarding potential manganese toxicity in children. Because potentially toxic levels of manganese are much lower for young children, exceeding the DRI upper limits for manganese set by the Institute of Medicine Food and Nutrition Board is relatively easy.

Publications

  • Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Dobbs, J.C. and Titchenal, C.A. 2016. Critical Importance of Balancing Public Health Nutrition Messages with Nutrition Science. Hawaii Public Health Conference, October 13, 2016. Honolulu, HI.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: LeMoon, T., Titchenal, C.A., Dunn, M.A., and Dobbs, J. 2016. Manganese Toxicity from Healthy Diets. Hawaii Public Health Conference, October 13, 2016. Honolulu, HI. (Poster)


Progress 10/01/15 to 09/30/16

Outputs
Target Audience:Target audiences reached this year include: university classroom instruction and outreach activities including newspaper audience, Hawaii State Farm Fair visitors (families), university students, staff and faculty. The Honolulu Star-Advertiser is Hawaii's major newspaper and is ranked 12th out of the 15 largest daily newspapers in the U.S. Combined daily print and online digital circulation exceeds 152,000 copies. Also nutrition researchers and health professionals were reached via one graduate student presentation at our college student research symposium and by an oral presentation at a western states regional conference. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Even with Internet access, few students today understand how to find nutrient composition information. This project has allowed for training of graduate students and undergraduate students in finding nutrient databases and pertinent references from around the world. How have the results been disseminated to communities of interest?Main vehicles for outreach included: Hawaii State Farm Fair demonstrations and handouts, university dorm presentations, University of Hawaii at Manoa Health Services individual counseling, and a Honolulu Star-Advertiser Health Options newspaper column. What do you plan to do during the next reporting period to accomplish the goals?We plan to 1) substantially increase the literature nutrient database regarding manganese content of foods and 2) obtain funding to do mineral analysis of commonly consumed processed foods as well as locally produced foods that are potentially high in manganese.

Impacts
What was accomplished under these goals? Databases and literature containing food composition data on manganese is fairly extensive. Development of a mineral database has been started with foods from the Total Diet Study included as well as six additional databases and research literature.Most resources do not contain manganese values for processed foods that are commonly consumed today. Many of these processed foods contain ingredients that likely contain high levels of manganese, as well as aluminum, another neurotoxic mineral.

Publications

  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2016 Citation: Dobbs, J., LeMoon, T., Dunn, M.A., and Titchenal, C.A. 2016. Identifying Groups at Greatest Risk for Potential Manganese Toxicity from Healthy Diets Regional Health Disparities in the Mountain West. Mountain West CTR-IN Third Annual Meeting June 6 - 8, 2016.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: LeMoon T., Titchenal C.A., Dunn, M.A., and Dobbs, J. 2016 Manganese Toxicity from Healthy Diets. CTAHR-COE Student Research Symposium. University of Hawaii.
  • Type: Other Status: Published Year Published: 2016 Citation: Titchenal, C.A., Dobbs, J.C. 2016. Too much of a healthy diet can lead to severe problems. Honolulu Star-Advertiser newspaper, April 26, 2016.