Source: UNIV OF MINNESOTA submitted to NRP
SYSTEMATICS OF LEPIDOPTERA (PARTICULARLY NOCTUOIDEA) AND OTHER ARTHROPODS
Sponsoring Institution
State Agricultural Experiment Station
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1006875
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 1, 2015
Project End Date
Jan 31, 2017
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIV OF MINNESOTA
(N/A)
ST PAUL,MN 55108
Performing Department
Entomology
Non Technical Summary
The two general objectives of this project are to discover and document biological diversity that is still unknown (or poorly described) in the tiger moth (Arctiidae) and cutworm moth (Noctuidae) families. These insects are chosen because they represent a significant component of the insects that occur in our forests, prairies, grasslands and other land areas. These moths, especially their caterpillars, are often a key component of food chains - consumed by vertebrates and other insects. The assessment of environmental roles connects with National initiatives on identifying pollinators and creating identification tools for distinguishing native and non-native species.
Animal Health Component
0%
Research Effort Categories
Basic
100%
Applied
0%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
21131101130100%
Goals / Objectives
The two general objectives of this project are: 1. Conduct systematic studies of tiger moths (Arctiidae: Noctuoidea); 2. Conduct systematic studies of Lepidoptera, with a focus on Noctuoidea. These focal taxa are chosen because they represent a significant component of the terrestrial insect fauna, and as such, are often consumed by vertebrates and other insects. The assessment of environmental roles connects with National initiatives on identifying pollinators and creating identification tools for distinguishing native and non-native species.For objectives 1 and 2, clarification of Noctuoidea phylogenetic relationships is essential for accurate identification and assessing their environmental role. For objective 1, I am focusing on four sub-objectives, 1A. preliminary molecular studies of Lithosiinae (lichenivorous tiger moths) who are potential bioindicators in terrestrial ecosystems; 1B. a genomics study of Arctiinae (tiger moths); 1C. analysis of tiger moth plant host associations, defense and mating modes within this framework; and 1D. continued development of the research program on the systematics of Arctiinae with an emphasis on the genera Euchaetes, Viviennea, and Ormetica. For objective 2, I am focusing on the systematic relationships of quadrifine noctuids in collaboration with the lab of Dr. Wahlberg (U. Turku Finland), Dr. Zaspel (Purdue U.) and Dr. Kawahara (U of Florida). Continued MAES/Department funding is necessary to support morphological work (supplies), collaboration through travel (National meetings), and dissemination of results (page charges).
Project Methods
The proposed approach uses morphological, biological, biogeographical and molecular data to classify and characterize difficult species limits, and resolve relationships among higher taxa (genera, tribes, etc). To generate these data, specimens are borrowed from museum and frozen tissue collections and these materials are supplemented by field work in collaboration with colleagues nationally and internationally. Data are collected using a variety of visualization techniques for morphology (light microscopy, SEM) and molecular sequencing of nuclear and mitochondrial genes. Resulting data are analyzed within a phylogenetic framework using appropriate methods for the taxonomic level (population, species, higher level) and for data type. For morphological data, parsimony and Bayesian-based methods are employed, and for molecular data model-based methods are employed (e.g., maximum likelihood, Bayesian, coalescent-based approaches). Whenever possible, a total evidence approach (multiple data sets of nuclear and mitochrondrial genes, morphology of adults and immature stages) are employed. Results will be disseminated through papers, monographs and the web (e.g., Encyclopedia of Life, Tree of Life, Barcodes of Life, Genbank). Traditional specimen and frozen tissue vouchers will be deposited in the UMSP Insect Collection, University of Minnesota (St. Paul), Purdue University (frozen tissues) and the Smithsonian Natural History Museum collection.

Progress 07/01/15 to 01/31/17

Outputs
Target Audience:Two audiences were targeted, researchers and the general public, including families and K-12 students. Scientific posters and presentations were given at the Entomol. Soc. Am. meetings; two research papers were published, and my lab participated in the delivery of a honeybee curriculum for Minneapolis schools. Changes/Problems:Although this project was inititated in July 2015, with the departure of the PI, Dr. Susan Weller (Sept. 2015), from the Univ. of Minnesota, to the Univ. of Nebraska, we request that the information provided serve as the FINAL Report. This project will not continue with another faculty member, and is now complete. Report was updated, and reviewed by Dr. W. Hutchison (dept. chair), Dec. 2015. What opportunities for training and professional development has the project provided?This project resulted in the training of a M.S. graduate student, who completed her degree in 2015 (Heather Hendrickson). How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?This project ended in 2015, due to the PI departure from the University of Minnesota, to the University of Nebraska.

Impacts
What was accomplished under these goals? I completed research with several colleagues on obtaining next generation sequencing data set for representative arctiines. The evolutionary analysis of tigermoth - host plant co-evolution is published (Zaspel et al. 2014 PlosOne) and a second manuscript on the evolution of moth defense and mating behaviors, based on a more intense sampling of lineages is Report Date 12/22/2015 Page 1 of 3 Accession No. 189216 Project No. MIN-17-022 in preparation; with several papers publlished in 2015. The Euchaetes study has been completed, and and as part of the M.S. thesis is filed (2015), manuscript preparation will commence. I also collaborated with the laboratory of Dr. Brian Aukema and his graduate students on Asian gypsy moth populations in north-eastern Minnesota. Dr. Roe has submitted our manuscript on pyralid moths and we are waiting on the review process.

Publications

  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Roe, A.D. et al. Phycitinae phylogeny based on two genes, with implications for morphological trait evolution and Heinrich's tribal classification. J. of Lepidopterists' Society. 69(3): 157-172.
  • Type: Theses/Dissertations Status: Published Year Published: 2015 Citation: Hendrickson, H. "Revision of the tiger moth genus Euchaetes", M.Sc. Thesis, Univ. of Minnesota.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: Zaspel, J.M., SJ Weller, and M. Epstein. Origin of the hungry caterpillar: Evolution of fasting in slug moths (Insecta: Lepidoptera: Limacodidae). Molecular Phylogenetics and Evolution (in press).


Progress 07/01/15 to 09/30/15

Outputs
Target Audience:Two audiences were targeted, researchers and the general public, including families and K-12 students. Scientific posters and presentations were given at the Entomol. Soc. Am. meetings; two research papers were published, and my lab participated in the delivery of a honeybee curriculum for Minneapolis schools. Changes/Problems:Although this project was inititated in July 2015, with the departure of the PI, Dr. Susan Weller (Sept. 2015), from the Univ. of Minnesota, to the Univ. of Nebraska, we request that the information provided serve as the FINAL Report. Thisproject will not continue with another faculty member, andis now complete. Report was updated, and reviewed by Dr. W. Hutchison (dept. chair),Dec. 2015. What opportunities for training and professional development has the project provided?This project resulted in the training of a M.S. graduate student, who completed her degree in 2015 (Heather Hendrickson). How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?This project ended in 2015, due to the PI departure from the University of Minnesota, to the University of Nebraska.

Impacts
What was accomplished under these goals? I completed researchwith severalcolleagues on obtaining next generation sequencing data set for representative arctiines. The evolutionary analysis of tigermoth - host plant co-evolution is published (Zaspel et al. 2014 PlosOne) and a second manuscript on the evolution of moth defense and mating behaviors, based on a more intense sampling of lineages is Report Date 12/22/2015 Page 1 of 3 Accession No. 189216 Project No. MIN-17-022 in preparation; with several papers publlished in 2015.The Euchaetes study has been completed, and and as part of the M.S.thesis is filed (2015), manuscript preparation will commence. I also collaborated with the laboratory of Dr. Brian Aukema and his graduate students on Asian gypsy moth populations in north-eastern Minnesota. Dr. Roe has submitted our manuscript on pyralid moths and we are waiting on the review process.

Publications

  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Roe, A.D. et al. Phycitinae phylogeny based on two genes, with implications for morphological trait evolution and Heinrich's tribal classification. J. of Lepidopterists' Society. 69(3): 157-.
  • Type: Theses/Dissertations Status: Published Year Published: 2015 Citation: Hendrickson, H. "Revision of the tiger moth genus Euchaetes", M.Sc. Thesis, Univ. of Minnesota.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: Zaspel, J.M., SJ Weller, and M. Epstein. Origin of the hungry caterpillar: Evolution of fasting in slug moths (Insecta: Lepidoptera: Limacodidae). Molecular Phylogenetics and Evolution (in press).