Source: UNIV OF MINNESOTA submitted to
NANOTECHNOLOGY SOLUTIONS FOR FOOD ENGINEERING, SAFETY, HEALTH AND ENVIRONMENT
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1006789
Grant No.
(N/A)
Project No.
MIN-12-091
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Oct 1, 2015
Project End Date
Sep 30, 2018
Grant Year
(N/A)
Project Director
Abbas, AB.
Recipient Organization
UNIV OF MINNESOTA
(N/A)
ST PAUL,MN 55108
Performing Department
Bioproducts & Biosystems Engineering
Non Technical Summary
The proposed research focuses on using nanotechnology-based solutions to address current challenges in food safety, food spoilage, food defense, precision agriculture, and environmental remediation. Over the next three years, we seek to develop new tools, sensors and devices to respond to four major needs: rapid and sensitive detection of foodborne pathogens, intelligent and functional food packaging, user-friendly devices for the analysis of soil quality in agricultural fields, and novel materials for mercury and contaminant removal from water and gas effluents.
Animal Health Component
0%
Research Effort Categories
Basic
10%
Applied
30%
Developmental
60%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
40472992020100%
Knowledge Area
404 - Instrumentation and Control Systems;

Subject Of Investigation
7299 - Research equipment and methods, general/other;

Field Of Science
2020 - Engineering;
Goals / Objectives
OBJECTIVESThe proposed research aims at developing new tools and solutions for consumers, producers, manufacturers and food inspectors to enable a better control over food quality along the food supply chain. Specifically, we will use nanotechnology and nanoengineering to achieve the following objectives:Foodborne Pathogen Detection: Develop biosensors and bioassays for rapid detection of foodborne pathogens and spoilage agents, including bacteria, viruses and fungi.Food Packaging: Develop new active and responsive food packaging systems to provide consumers with real-time information on food quality and improve the shelf life of food products.Precision Agriculture: Design systems for precision agriculture, particularly we seek to provide farmers and agricultural engineers with a portable soil sensor for on-site and real-time measurement of the amount of nitrate ()-nitrogen in soils. The sensor will be designed to be hand-held or mounted on a vehicle for large area monitoring, which will inform decisions on effective adjustment of the rate and timing of fertilizer input.Environmental monitoring and remediation: Provide nanotechnology-based solution for rapid contaminant detection and remediation of natural resources including mercury removal from water and flue gas.
Project Methods
The use of nanotechnology involves a number of different methods:- Synthesis of nanomaterials: a number of nanomaterials will be synthesized including: nanoliposomes (100-400 nm), gold nanostructures (nanospheres, nanorods, nanowires, ..), selenium nanmaterials, and nanobiomaterials.- Nanofabrication: fabrication of electrods for electrochemical sensors can be performed at the Minnesota Nanocenter- Material Characterization: The techniques includes microscopy (Optical, SEM, TEM) and spectroscopy (Raman, FTIR UV-visible)- Device application and charcaterization: We will use plasmonic sensors and electrochemical sensors for detection and analysis.

Progress 10/01/15 to 09/30/18

Outputs
Target Audience:- Environmental agenices and organization - Watsewater treatment industry - Food safety agencies and organizations - Food industry - Microbial diagnostic industry Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?- Trained 20 undergraduate students, 5 graduate students (graduated two students), and 7 postdoctoral research associates on different field of nanotechnology applications including biosensors for food safety and nanosrobents for wayter remediation. - Initiated and maintained an Academic mentorship program for K12 students from underrepresented communities since 2017. - Continuing collaboration with the Honors Mentor Connection program with a local high school for the promotion and mentoring of talented students. How have the results been disseminated to communities of interest?- The results have been disseminated by giving 2 invited seminars and presenting research results in over 6 national and international conferences. We have also issued press releases and our research was highlighted in the online news media including: 1. Press release: "Researchers use green gold to rapidly detect and identify harmful bacteria", Phys.Org, July 31, 2018. Link to the interview 2. TV news broadcast: Jeff Edmondson, "New tech may speed up Oak wilt detection", KARE11, November 14, 2017. Link to the interview -Produced 16 publications (2 under review), including 2 book chapters, 9 research articles in journals with impact factors higher than 5 and 2 articles in journals with impact factor higher than 12. Five of the articles have been highlighted in the news media and published as cover articles. -Organized 3 Research Exhibits for the public and presented findings in 14 invited seminars and 36 conferences and 10 interviews in the news media. What do you plan to do during the next reporting period to accomplish the goals?- The next year will be focused on 3 specific projects: 1- Develop a new platform for environmental DNA collection 2- Develp a portable platform for pak wilt detection (microbial identificvation) 3- Work in collaboration with Claros Technologies Inc. (STTR and SBIR projects) to develop new products for water remediations

Impacts
What was accomplished under these goals? 1- Foodborne pathogen detection: - Developed different technologies for microbial detection. We have filled 6 provisional patents and have been awarded the first patent (U.S. Patent No. 10,006,906) on June 26, 2018. 2- Environmental monitoring and remediation: - Developed new sorbents to remove mercury and phosphorus from wastewater. We have also founded a new startup technology company "Claros Technologies (www.clarostech.com) to commercialize the diffeent sorbents for water remediationa dn wastewater treatment. 3- The food packaging and precision agriculture projects were dropped as there was no industrial interest in the developed technologies for pakcaging and nitrate measurement.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Bui P., Brockgreitens J. and Abbas A.*, "Gold Nanoplate-Enhanced Chemiluminescence and Macromolecular Shielding for Rapid Microbial Diagnostics", Advanced Healthcare Materials, 2018, 7, 1701506
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2019 Citation: Nanomaterials for Biodiagnostics, Invited talk, 257th American Chemical Society National Meeting & Exposition, March 31  April 4, 2019, Orlando, Florida.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2019 Citation: Abbas A., A universal nanocoating technology for functional textile, Invited talk, 257th American Chemical Society National Meeting & Exposition, March 31  April 4, 2019, Orlando, Florida.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Brockgreitens j., Bui M-P N. and Abdennour Abbas, Microbial Sensing Using Gold Nanoplate-Enhanced Chemiluminescence and Macromolecular Shielding, 2018 NanoEngineering for Medicine and Biology Conference, Los Angeles, CA, August 21-24, 2018.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Ke Xu, Aiqin Fang, Minh-Phuong Ngoc Bui, John Brockgreitens and Abdennour Abbas, Rapid microbial screening using cell nanocoating, 2018 NanoEngineering for Medicine and Biology Conference, Los Angeles, CA, August 21-24, 2018
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: John Brockgreitens, Minh-Phuong Ngoc Bui and Abdennour Abbas, Nanodiagnostics for Rapid Microbial Detection, 2018 ASABE Annual International Meeting, Detroit, MI, July 29-August 1, 2018.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Ke Xu, Aiqin Fang, Minh-Phuong Ngoc Bui, John Brockgreitens and Abdennour Abbas, Rapid microbial screening using cell nanocoating, Biosensors 2018, Miami, FL, June 12-15, 2018
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: John Brockgreitens, Minh-Phuong Ngoc Bui and Abdennour Abbas, Gold nanoplate-enhanced chemiluminescence for rapid microbial detection, Biosensors 2018, Miami, FL, June 12-15, 2018
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: John Brockgreitens, Fatemeh Heidari and Abdennour Abbas, Rapid Removal and Recovery of Phosphorus from Water using a NanoIron Sponge, 2018 Environmental & Water Resources Congress, Minneapolis, MN, June 3-7, 2018
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: John Brockgreitens, Fatemeh Heidari and Abdennour Abbas, Arsenic Removal from Water with a Copper Nanoparticle Sorbent, 2018 Environmental & Water Resources Congress, Minneapolis, MN, June 3-7, 2018
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Heidari F. and Abbas A., Rapid removal and recovery of phosphorus from water using a nanoiron sponge, 2018 Atlantic Basin Conference on Chemistry (ABCChem), Canc�n, Mexico, 23-26 January 2018.


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:Environmental agencies and organizations (eg. EPA), public health professionals, food industry, water industry Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?- One student (Ms. Snober Ahmed) graduated with a PhD Degree and she is now a Research Associate at McMaster University (Canada) - One student graduated with a Masters Degree and she received a graduatefelloship from McGill University (Canada) - Three Postdoctoral Research Assicates: Aiqin Fang, Renu Signh and Phuong Bui left the lab for the following positions: Dr . Aiqin Fang: Aiqin left our group in 2017 for a position as an Assistant Professor at Vincennes University (Indiana, USA). Dr. Renu Singh: Renu left our group in 2017 for a Scientist position at the CSIR-Central Food Technological Research Institute (India). Dr Phuon Bui Renu left our group in 2017 for a Lead Scientist position at a startup company in Florida, How have the results been disseminated to communities of interest?The results were dessiminated by publications but also by many new repoorts in the media in 2017 including: "University researchers invent nano-sponge to soak-up pollution" (Interview), in Star Tribune, March 25, 2017. Our research on a mercury sponge was featured in a news story in ABC News channel 5, Minnesota Daily (March 12, 2017), "New technology may speed up Oak wilt detection". KARE 11 (TV new report), November 14, 2017. What do you plan to do during the next reporting period to accomplish the goals?- Finalize the spin-off of our new startup company on clean water technologies early 2018. - Prepare a new startup company focused on rapid diagnostic technologies by early 2019.

Impacts
What was accomplished under these goals? - Environment: A platform technology was developed to clean water from heavy metals and organic pollutants in a faster and cheaper way. A new startup company is under incorporation. - New detection technologies were developed for foodborne pathogens and plant/tree pathogens. Three patents have been filled.

Publications

  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Plasmonic cell nanocoating: a new concept for rapid microbial screening K Xu, MPN Bui, A Fang, A Abbas Analytical and bioanalytical chemistry 409 (27), 6305-6314
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Rapid and PCR-free DNA Detection by Nanoaggregation-Enhanced Chemiluminescence R Singh, A Feltmeyer, O Saiapina, J Juzwik, B Arenz, A Abbas Scientific reports 7 (1), 14011
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Microbial separation from a complex matrix by a hand-held microfluidic device R Singh, J Brockgreitens, O Saiapina, Y Wu, A Abbas Chemical Communications 53 (78), 10788-10791
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: A Nanoselenium Sponge for Instantaneous Mercury Removal to Undetectable Levels S Ahmed, J Brockgreitens, K Xu, A Abbas Advanced Functional Materials 27 (17)
  • Type: Theses/Dissertations Status: Published Year Published: 2017 Citation: Sorbent Nanotechnologies for Water Cleaning, Ahmed, Snober Issue Date: 2017-07


Progress 10/01/15 to 09/30/16

Outputs
Target Audience:During this period, the research group conducted two main projects: 1- Rapid microbial detection technologies for food safety Targeted audience: Food industry, diagnostic laboratories, public agencies (FDA, USDA) 2- Water cleaning technologies Targeted audience: Wastewater treatment industry, public agencies (EPA, USDA) Changes/Problems:N/A What opportunities for training and professional development has the project provided?- Trained 12 undergraduate students, 4 graduate students and 4 postdoctoral research associates. How have the results been disseminated to communities of interest?Public Media coverage: "Accelerating Detection: from Farm to Forks", (Report and Interview), in Food Technology Magazine, December 2015. "U's business ties expected to expand", in Minnesota Daily, February 23, 2015 "Device saves money, time", in Minnesota Daily, January 21, 2015. Our research on Bioresponsive Packaging was featured in a news story in ABC News channel 5, Minnesota Daily (July 30, 2014), and UMN Brief (December 2014). Conferences: A. Snober and A. Abbas*, American Chemical Sicet, Fall meeting, Boston , August 12, 2015. M-P N. Bui and A. Abbas*, American Chemical Sicet, Fall meeting, Boston , August 12, 2015. J. Brockgreitens and A. Abbas*,Global Congress on NanoEngineering for Medicine and Biology, Minneapolis, 2015 A. Snober and A. Abbas*,Global Congress on NanoEngineering for Medicine and Biology, Minneapolis, 2015 M-P N. Bui, A. Snober and A. Abbas*,Global Congress on NanoEngineering for Medicine and Biology, Minneapolis, 2015 J. Brockgreitens and A. Abbas*, "On-site and Naked-eye Detection of Organophosphate Toxins in Food and Water Supplies", Food Defense Conference, September 15-19, 2014.(Poster) M.-P. Bui and A. Abbas*, "Simple and Rapid Detection of Paraoxon Derivatives in Drinking Water", Food Defense Conference, September 15-19, 2014. (Oral presentation). J. Brockgreitens, K. Xu, S. Ahmed and A. Abbas*, " Chromogenic Coating for Bioresponsive Food Packaging", 13th Advanced Coating Fundamentals Symposium, October 7-9, 2014. S. Ahmed and A. Abbas*, " Paper-based Biosensors", 13th Advanced Coating Fundamentals Symposium, October 7-9, 2014. What do you plan to do during the next reporting period to accomplish the goals?- Growing the intectual property portfolio by inventing and patenting new technologies - Founding one start up company for rapid diagnostics and one for water cleaning - Publishing papers and attending conferences

Impacts
What was accomplished under these goals? Objectives: Foodborne Pathogen Detection: A portfolio of diagnostic technologies has been developed, and a start up company is under creation to commercialize the technology. Food Packaging: A responsive food packaging have been developed. However our industrial collaboration evaluated the technology and decided it will be too costly to proceed. Environmental monitoring and remediation: A new technology have been developed and a start up company is under creation to commercialize the technology Awards: Best Poster Award at the Food Defense Conference, organized on September 18-19, 2014, by the National Center for Food Protection and Defense.(Student: J. Brockreitens) Award of Merit at theGlobal Congress on NanoEngineering for Medicine and Biology, 2015(student: Snober Ahmed). Award of Merit at theGlobal Congress on NanoEngineering for Medicine and Biology, 2015(student: John Brockgreitens).

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: M-P. N. Bui, J. Brockgreitens, S. Ahmed, and Abdennour Abbas*, "Dual Detection of Nitrate and Mercury in Water using Disposable Electrochemical Sensors", Biosensors and Bioelectronics, 2016, 15;85:280-6
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Snober Ahmed, John Brockgreitens, Ke Xu, Abdennour Abbas, "Sponge-supported synthesis of colloidal selenium nanospheres", Nanotechnology, 2016, 18;27(46):465601
  • Type: Journal Articles Status: Under Review Year Published: 2017 Citation: Snober Ahmed, John Brockgreitens, Ke Xu, Abdennour Abbas, "A nanoselenium sponge for instantaneous mercury removal to undetectable levels", Submitted (2016)
  • Type: Journal Articles Status: Under Review Year Published: 2017 Citation: Ke Xu, Phuong Bui, Aiqin Fang and A. Abbas., "Cell Nanocoating for Rapid Microbial Screening", Submitted (2016)
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Minh-Phuong Ngoc Bui, Snober Ahmed, and Abdennour Abbas*, "Single Digit Pathogen and Attomolar Detection with the Naked Eye using Liposome-Amplified Plasmonic Immunoassay", Nano Letters, 2015, DOI: 10.1021/acs.nanolett.5b02837
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: J. Brockgreitens and A. Abbas*, "Responsive Food Packaging: Recent Progress and Technological Prospects", Comprehensive Reviews in Food Science and Food Safety, 2015, DOI: 10.1111/1541-4337.312174.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: S. Ahmed, M-P N. Bui and A. Abbas, "Paper-based Chemical and Biological Sensors: Engineering Aspects", Biosensors and Bioelectronics, 2015, DOI: 10.1016/j.bios.2015.09.038
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: M-P. Bui and A. Abbas*, "Simple and Rapid Colorimetric Detection of p-Nitrophenyl Substituent Organophosphorous Nerve Agents", Sensors & Actuators B, 2014, DOI: 10.1016/j.snb.2014.10.010
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: J. Brockgreitens, S. Ahmed and A. Abbas*, "Kinetic Analysis of a-Cyclodextrin Interactions using Polydiacetylene Liposomes", Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2014, DOI: 10.1007/s10847-014-0470-y.
  • Type: Book Chapters Status: Published Year Published: 2016 Citation: Bui, Minh-Phuong Ngoc, Snober Ahmed, and Abdennour Abbas. "Chapter 14 Biosensors in Food Engineering." Food Engineering: Emerging Issues, Modeling, and Applications. Apple Academic Press, 2016. 321-334.
  • Type: Book Chapters Status: Published Year Published: 2016 Citation: Brockgreitens, John, and Abdennour Abbas. "Chapter 5 Food Packaging Technologies." Food Engineering: Emerging Issues, Modeling, and Applications. Apple Academic Press, 2016. 93-110.