Source: NORTH CAROLINA STATE UNIV submitted to NRP
MOLECULAR MECHANISMS REGULATING SKELETAL MUSCLE GROWTH AND DIFFERENTIATION
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1006656
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
NC-_old1184
Project Start Date
Oct 1, 2015
Project End Date
Sep 30, 2020
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
NORTH CAROLINA STATE UNIV
(N/A)
RALEIGH,NC 27695
Performing Department
Poultry Science
Non Technical Summary
Undertake studies aimed at gaining a fundamental understanding of muscle protein accretion in Poultry. Specifically, in vitro methodologies will be employed to understand the mechanisms that ammonia effects myoblast profiliferation, differentiation, and myotube size in culture. I have no funding under my leadership to supportavian animaluse. Existing lines of cells exist in the laboratory, we will use the existing cell lines to answer hypothesis. There is no fundingassociated withthe project to support any animal use. Therefore, we will exclusively use cell culture methodologies to support the project.
Animal Health Component
(N/A)
Research Effort Categories
Basic
100%
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3023230103025%
3023220103025%
3053230103025%
3053220103025%
Knowledge Area
302 - Nutrient Utilization in Animals; 305 - Animal Physiological Processes;

Subject Of Investigation
3220 - Meat-type chicken, live animal; 3230 - Turkey, live animal;

Field Of Science
1030 - Cellular biology;
Goals / Objectives
Characterize the signal transduction pathways that regulate skeletal muscle growth and metabolism including the influence of endogenous growth factors and various production practices. Characterize the cellular and molecular basis of myogenesis.
Project Methods
Cell and Molecular Biology Methods, Western Blotting, Real time PCR, Immunostaining, and Microscopy.

Progress 10/01/19 to 09/30/20

Outputs
Target Audience:Scientists study avian muscle biology, and cellular agriculture. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Scientific publications What do you plan to do during the next reporting period to accomplish the goals?Currently evaluating the effects of differing methionine sources on avian satellite cell proliferation and differentiation.

Impacts
What was accomplished under these goals? We have identified that fish and avian share the ability to metabolize ammonia to fuel skeletal muscle growth whereas ammonia is toxic to mammalian muscle. We have also shown that chick myoblasts have the ability to transdifferentiate to different lineages after injection to the blastoderm, but are essentially all myogenic in vitro.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Skeletal Muscle and the Effects of Ammonia Toxicity in Fish, Mammalian, and Avian Species: A Comparative Review Based on Molecular Research By:Miramontes, E (Miramontes, Emily)[ 1 ] ; Mozdziak, P (Mozdziak, Paul)[ 1 ] ; Petitte, JN (N. Petitte, James)[ 1 ] ; Kulus, M (Kulus, Magdalena)[ 2 ] ; Wieczorkiewicz, M (Wieczorkiewicz, Maria)[ 3 ] ; Kempisty, B (Kempisty, Bartosz)[ 2,4,5,6 ] View Web of Science ResearcherID and ORCID INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Volume: 21 Issue: 13 Article Number: 4641 DOI: 10.3390/ijms21134641 Published:JUL 2020 Document Type:Review
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Avian Satellite Cell Plasticity By:Jankowski, M (Jankowski, Maurycy)[ 1 ] ; Mozdziak, P (Mozdziak, Paul)[ 2 ] ; Petitte, J (Petitte, James)[ 2 ] ; Kulus, M (Kulus, Magdalena)[ 3 ] ; Kempisty, B (Kempisty, Bartosz)[ 1,3,4,5,6 ] View Web of Science ResearcherID and ORCID ANIMALS Volume: 10 Issue: 8 Article Number: 1322 DOI: 10.3390/ani10081322 Published:AUG 2020 Document Type:Article
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: In Vitro Cultures of Adipose-Derived Stem Cells: An Overview of Methods, Molecular Analyses, and Clinical Applications By:Jankowski, M (Jankowski, Maurycy)[ 1 ] ; Dompe, C (Dompe, Claudia)[ 2,3 ] ; Sibiak, R (Sibiak, Rafal)[ 1 ] ; Wasiatycz, G (Wasiatycz, Grzegorz)[ 4 ] ; Mozdziak, P (Mozdziak, Paul)[ 5 ] ; Jaskowski, JM (Jaskowski, Jedrzej M.)[ 6 ] ; Antosik, P (Antosik, Pawel)[ 4 ] ; Kempisty, B (Kempisty, Bartosz)[ 1,2,4,7,8 ] ; Dyszkiewicz-Konwinska, M (Dyszkiewicz-Konwinska, Marta)[ 1,9 ] View Web of Science ResearcherID and ORCID CELLS Volume: 9 Issue: 8 Article Number: 1783 DOI: 10.3390/cells9081783
  • Type: Theses/Dissertations Status: Published Year Published: 2020 Citation: MIRAMONTES, EMILY. Myogenic Response to Increasing Concentrations of Ammonia Differs Between Mammalian, Avian, and Fish Species. (Under the direction of Paul Mozdziak)
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Human Umbilical Vein Endothelial Cells (HUVECs) Co-Culture with Osteogenic Cells: From Molecular Communication to Engineering Prevascularised Bone Grafts By:Kocherova, I (Kocherova, Ievgeniia)[ 1 ] ; Bryja, A (Bryja, Artur)[ 1 ] ; Mozdziak, P (Mozdziak, Paul)[ 2 ] ; Volponi, AA (Volponi, Ana Angelova)[ 3 ] ; Dyszkiewicz-Konwinska, M (Dyszkiewicz-Konwinska, Marta)[ 1,4 ] ; Piotrowska-Kempisty, H (Piotrowska-Kempisty, Hanna)[ 5 ] ; Antosik, P (Antosik, Pawel)[ 6 ] ; Bukowska, D (Bukowska, Dorota)[ 6 ] ; Bruska, M (Bruska, Malgorzata)[ 1 ] ; Izycki, D (Izycki, Dariusz)[ 7 ] ...More View Web of Science ResearcherID and ORCID JOURNAL OF CLINICAL MEDICINE Volume: 8 Issue: 10 Article Number: 1602 DOI: 10.3390/jcm8101602 Published:OCT 2019
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Ammonia Induces a Myostatin-Mediated Atrophy in Mammalian Myotubes, but Induces Hypertrophy in Avian Myotubes By:Stern, RA (Stern, Rachel A.)[ 1 ] ; Dasarathy, S (Dasarathy, Srinivasan)[ 2,3 ] ; Mozdziak, PE (Mozdziak, Paul E.)[ 1 ] View Web of Science ResearcherID and ORCID FRONTIERS IN SUSTAINABLE FOOD SYSTEMS Volume: 3 Article Number: UNSP 115 DOI: 10.3389/fsufs.2019.00115 Published:DEC 18 2019 Document Type:Article
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Bone Regeneration, Reconstruction and Use of Osteogenic Cells; from Basic Knowledge, Animal Models to Clinical Trials By:Hutchings, G (Hutchings, Greg)[ 1,2 ] ; Moncrieff, L (Moncrieff, Lisa)[ 1,3 ] ; Dompe, C (Dompe, Claudia)[ 1,3 ] ; Janowicz, K (Janowicz, Krzysztof)[ 1,2 ] ; Sibiak, R (Sibiak, Rafal)[ 4 ] ; Bryja, A (Bryja, Artur)[ 2 ] ; Jankowski, M (Jankowski, Maurycy)[ 2 ] ; Mozdziak, P (Mozdziak, Paul)[ 5 ] ; Bukowska, D (Bukowska, Dorota)[ 6 ] ; Antosik, P (Antosik, Pawel)[ 7 ] ...More View Web of Science ResearcherID and ORCID JOURNAL OF CLINICAL MEDICINE Volume: 9 Issue: 1 Article Number: 139 DOI: 10.3390/jcm9010139 Published:JAN 2020
  • Type: Book Chapters Status: Published Year Published: 2020 Citation: Transgenic chicken/poultry birds: serving us for survival By:Golkar-Narenji, A (Golkar-Narenji, Afsaneh)[ 1 ] ; Petitte, JN (Petitte, James N.)[ 1 ] ; Mozdziak, PE (Mozdziak, Paul E.)[ 1 ] View Web of Science ResearcherID and ORCID GENOMICS AND BIOTECHNOLOGICAL ADVANCES IN VETERINARY, POULTRY, AND FISHERIES Edited by:Malik, YS; Barh, D; Azevedo, V; Khurana, SMP Pages: 211-221 DOI: 10.1016/B978-0-12-816352-8.00009-6 Published: 2020 Document Type:Article; Book Chapter
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Designing A Transgenic Chicken: Applying New Approaches toward A Promising Bioreactor By:Bahrami, S (Bahrami, Salahadin)[ 1 ] ; Amiri-Yekta, A (Amiri-Yekta, Amir)[ 1 ] ; Daneshipour, A (Daneshipour, Abbas)[ 1 ] ; Jazayeri, SH (Jazayeri, Seyedeh Hoda)[ 1 ] ; Mozdziak, PE (Mozdziak, Paul Edward)[ 2 ] ; Sanati, MH (Sanati, Mohammad Hossein)[ 1,3 ] ; Gourabi, H (Gourabi, Hamid)[ 1 ] View Web of Science ResearcherID and ORCID CELL JOURNAL Volume: 22 Issue: 2 Pages: 133-139 DOI: 10.22074/cellj.2020.6738 Published:SUM 2020 Document Type:Review
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: The Proliferation and Differentiation of Adipose-Derived Stem Cells in Neovascularization and Angiogenesis By:Hutchings, G (Hutchings, Greg)[ 1,2 ] ; Janowicz, K (Janowicz, Krzysztof)[ 1,2 ] ; Moncrieff, L (Moncrieff, Lisa)[ 1,3 ] ; Dompe, C (Dompe, Claudia)[ 1,3 ] ; Strauss, E (Strauss, Ewa)[ 4,5 ] ; Kocherova, I (Kocherova, Ievgeniia)[ 2 ] ; Nawrocki, MJ (Nawrocki, Mariusz J.)[ 2 ] ; Kruszyna, L (Kruszyna, Lukasz)[ 5 ] ; Wasiatycz, G (Wasiatycz, Grzegorz)[ 6 ] ; Antosik, P (Antosik, Pawel)[ 6 ] ...More View Web of Science ResearcherID and ORCID INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Volume: 21 Issue: 11 Article Number: 3790 DOI: 10.3390/ijms21113790 Published:JUN 2020
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation By:Dompe, C (Dompe, Claudia)[ 1,2 ] ; Kranc, W (Kranc, Wieslawa)[ 3 ] ; Jopek, K (Jopek, Karol)[ 2 ] ; Kowalska, K (Kowalska, Katarzyna)[ 2 ] ; Ciesiolka, S (Ciesiolka, Sylwia)[ 2 ] ; Chermula, B (Chermula, Blazej)[ 4 ] ; Bryja, A (Bryja, Artur)[ 3 ] ; Jankowski, M (Jankowski, Maurycy)[ 3 ] ; Perek, J (Perek, Joanna)[ 3 ] ; Jozkowiak, M (Jozkowiak, Malgorzata)[ 5 ] ...More View Web of Science ResearcherID and ORCID JOURNAL OF CLINICAL MEDICINE Volume: 9 Issue: 6 Article Number: 2006 DOI: 10.3390/jcm9062006 Published:JUN 2020 Document Type:Article


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:Target Audience have been other muscle biologists, stakeholders such as the national cattlewomen's association, governmental regualtory agencies such as USDA. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We have provided an opportunity for three Masters Student and a visiting scholar to undertake further professional development in the laboratory. How have the results been disseminated to communities of interest?Peer- Reviewed Publications Invited Speaking Engagements. What do you plan to do during the next reporting period to accomplish the goals?1. USDA-FSIS, August 14, 2019. 2. 4TH International Symposium on Food Science and Human Wellness, Ningbo China, August 1, 2019. 3. International Society of Food Protection, July 2019. 4. Reciprocal Meat Conference, June 2019. 5. National Cattlewomen's Association, Webinar, May 2019. 6. Food Research Institute University of Wisconsin-Madison, December 2018. 7. Turkey Days, Wilmington NC, October 2018. 8. BTEC, North Carolina State University, October 2018.

Impacts
What was accomplished under these goals? We have identified a new mechanism of cellular metabolism where ammonia is metabolized by avian skeletal muscle, but induces atrophy in mammalian skeletal muscle.

Publications

  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Glutamine synthetase in avian muscle contributes to a positive myogenic response to ammonia compared with mammalian muscle By: Stern, Rachel Allysa; Mozdziak, Paul E. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY Volume: 317 Issue: 1 Pages: R214-R221 Published: JUL 2019
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Differential ammonia metabolism and toxicity between avian and mammalian species, and effect of ammonia on skeletal muscle: A comparative review By: Stern, Rachel A.; Mozdziak, Paul E. JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION Volume: 103 Issue: 3 Pages: 774-785 Published: MAY 2019
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Z-band and M-band titin splicing and regulation by RNA binding motif 20 in striated muscles By: Chen, Zhilong; Maimaiti, Rexiati; Zhu, Chaoqun; et al. JOURNAL OF CELLULAR BIOCHEMISTRY Volume: 119 Issue: 12 Pages: 9986-9996 Published: DEC 2018
  • Type: Journal Articles Status: Under Review Year Published: 2019 Citation: Stern, R. A., S. Dasarathy, and P. E. Mozdziak, 2019. Ammonia induces a myostatin-mediated atrophy in mammalian myotubes, but induces hypertrophy in avian myotubes. Frontiers-Cellular Agriculture (Submitted).


Progress 10/01/17 to 09/30/18

Outputs
Target Audience:Poultry Scientists and Muscle Biologists Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?A PhD student was trained. How have the results been disseminated to communities of interest?Dr. Mozdziak was an invited Speaker at Turkey Days in Wilmington directly engaging stakeholders on October 3, 2018. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? An understanding of the differing signaling mechanisms related to myogenesis was elucidated for avian compared to mammalian species.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: 6. Maaenia, Y., M, S. B. Leeb, I.H. Ismaila, P. Mozdziak, and Y. S., Kim, 2018. Cloning of Japanese quail (Coturnix Japonica) follistatin and production of bioactive quail follistatin288 in E. coli International Journal of Poultry Science (In Press).
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: The evolution of chicken stem cell culture methods By:Farzaneh, M (Farzaneh, M.)[ 1 ] ; Attari, F (Attari, F.)[ 2 ] ; Mozdziak, PE (Mozdziak, P. E.)[ 3 ] ; Khoshnam, SE (Khoshnam, S. E.)[ 4,5 ] BRITISH POULTRY SCIENCE Volume: 58 Issue: 6 Pages: 681-686 DOI: 10.1080/00071668.2017.1365354 Published:2017
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: 1. Krieger, J. A. Yesinovskiy, P. Mozdziak, and M. Kim, 2018. Developing scaleable production methods for cultured pork and turkey meat: beyond. Proceedings of the Recprocial Meat Conference.
  • Type: Theses/Dissertations Status: Submitted Year Published: 2018 Citation: Myogenic response to ammonia differs between avian and mammalian species Rachel Allyssa Stern PhD
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: The method of chicken whole embryo culture using the eggshell windowing, surrogate eggshell and ex ovo culture system By: Farzaneh, M.; Attari, F.; Khoshnam, S. E.; et al. BRITISH POULTRY SCIENCE Volume: 59 Issue: 2 Pages: 240-244 Published: 2018 Find Text @ NCSU Full Text from Publisher View Abstract
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Characterization of TTN Novex Splicing Variants across Species and the Role of RBM20 in Novex-Specific Exon Splicing By: Chen, Zhilong; Song, Jiangping; Chen, Liang; et al. GENES Volume: 9 Issue: 2 Article Number: 86 Published: FEB 2018
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Z-band and M-band Titin Splicing and Regulation by RBM20 in Striated Muscles By: Rexiati, Maimaiti; Chen, Zhilong; Zhu, Chaoqun; et al. Conference: Annual Meeting of Amer-Assoc-of-Anatomists (AAA), Amer-Physiol-Soc (APS), Amer-Soc-for-Biochemistry-and-Mol-Biol (ASBMB), Amer-Soc-for-Investigat-Pathol (ASIP), Amer-Soc-for-Pharmacol-and-Experimental-Therapeut (ASPET) on Experimental Biology (EB) Location: Amer Assoc Anatomists, San Diego, CA Date: APR 21-25, 2018 Sponsor(s): Amer Physiol Soc; Amer Soc Biochemistry & Mol Biol; Amer Soc Investigat Pathol; Amer Soc Pharmacol & Experimental Therapeut FASEB JOURNAL Volume: 32 Issue: 1 Supplement: S Meeting Abstract: lb19 Published: APR 2018 Find Text @ NCSU View Abstract


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:Target audience are other researchers who study the mechanisms governing skeletal muscle growth. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Graduate Student trained How have the results been disseminated to communities of interest?publications What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Increased myostatin expression, resulting in muscle loss, has been associated with hyperammonemia in mammalian models of cirrhosis. However, there is evidence that hyperammonemia in avian embryos results in a reduction of myostatin expression, suggesting a proliferative myogenic environment. The present in vitro study examines species differences in myotube and liver cell response to ammonia using avian and murine-derived cells. Primary myoblasts and liver cells were isolated from embryonic day 15 and 17 chick embryos to be compared with mouse myoblasts (C2C12) and liver (AML12) cells. Cells were exposed to varying concentrations of ammonium acetate (AA; 2.5, 5, or 10 mM) to determine the effects of ammonia on the cells. Relative expression of myostatin mRNA, determined by quantitative real-time PCR, was significantly increased in AA (10 mM) treated C2C12 myotubes compared to both ages of chick embryonic myotube cultures after 48 h (P < 0.02). Western blot analysis of myostatin protein confirmed an increase in myostatin expression in AA-treated C2C12 myotubes compared to the sodium acetate (SA) controls, while myostatin expression was decreased in the chick embryonic myotube cultures when treated with AA. Myotube diameter was significantly decreased in AA-treated C2C12 myotubes compared to controls, while avian myotube diameter increased with AA treatment (P < 0.001). There were no significant differences between avian and murine liver cell viability, assessed using 2', 7'- bis-(2-carboxyethyl)-5-(and-6-)-carboxyfluorescein, acetoxymethyl ester, when treated with AA. However, after 24 h, AA-treated avian myotubes showed a significant increase in cell viability compared to the C2C12 myotubes (P < 0.05). Overall, it appears that there is a positive myogenic response to hyperammonemia in avian myotubes compared to murine myotubes, which supports a proliferative myogenic environment.

Publications

  • Type: Journal Articles Status: Published Year Published: 2017 Citation: 9. Bazgir, B., R. Fathei, M. Rezazadeh Valoujerd, P. Mozdziak, and A. Asgari, 2017. Satellite cell contribution to cxercise mediated muscle hypertrophy and repair: A review article. Cell Journal 18: 473-484.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Stern, R., A. S. Dasarathy, and P.E. Mozdziak, 2017. Ammonia elicits a different myogenic response in avian and murine myotubes. In Vitro Cell Dev. Biol. Animal. 53: 99-110.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: 7. Farzaneh, M, S. N. Hossani, P.E. Mozdziak, and H. Baharvand. 2017. Avian embryos and related cell lines: a convenient platform for recombinant proteins and vaccines production. Biotechnology Journal 12: DOI 10.1002/biot.201600598
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Farzaneh, M, S. E. Khoshnam, and P.E. Mozdziak. 2017. Concise review: avian multipotent stem cells as a novel tool for investigating cell based therapies. J. Dairy Veterinary Animal Research 5: DOI: 10.15406/jdvar.2017.05.00125
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Farzaneh, M, S. E. Khoshnam, and P.E. Mozdziak, 2017. The evolution of chicken stem cell culture methods. British Poultry Science.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2017 Citation: 4. Zand-vakili, M. A. Golkar-Narenji, H. Eimani, and P. E. Mozdziak, 2017. An in vitro study on cumulus oocyte complexes COCs and follicles of autotransplanted mouse ovaries with the effect of VEGF. Journal of the Turkish German Gynecological Association (In Press).
  • Type: Journal Articles Status: Under Review Year Published: 2018 Citation: Farzaneh, M., F. Attari, S. E. Khoshnam, and P. E. Mozdziak, 2017. Chicken whole embryo culture system, the strong in-vitro model, British Poultry Science (Submitted).
  • Type: Journal Articles Status: Under Review Year Published: 2018 Citation: Shoeibi, S. P. Mozdziak, A. Golkar-Narenji, 2017. Biogenesis of selenium nanoparticles using green chemistry. Topics in Current Chemistry (Submitted).


Progress 10/01/15 to 09/30/16

Outputs
Target Audience:We have reached other investigators with an interest in avian muscle metabolism and myogenesis both in the US and internationally. We have also reached a group of individuals interested in employing myogenic stem cells as a therapeutic treatment for losses in muscle function. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?I have a graduate student whom does not derive any salary support from NC-1084 funds performing her PhD research on the topic of ammonia utilization by avian myotube cultures. How have the results been disseminated to communities of interest?Graduate student made a presentation at the Annual Meeting of the Poultry Science Association. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Ammonia, is well known to be cytotoxic to mammaliam muscle cells and results in an upregulation of myostatin. However, we have clearly demonstrated that ammonia applied at the same concentration to avian myotube cultures represses myostatin expression and is metabolized by the muscle cells.

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Stern, R.A., Dasarathy, S. & Mozdziak, P.E Ammonia elicits a different myogenic response in avian and murine myotubes. . In Vitro Cell.Dev.Biol.-Animal (2016). doi:10.1007/s11626-016-0088-z.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2017 Citation: 2. Behzad Bazgir, Rouhollah Fathi, Mojtaba Rezazadeh Valojerdi, Paul Mozdziak, Alireza Asgari. 2016. Satellite Cells Contribution to Exercise Mediated Muscle Hypertrophy and Repair. Cell Journal (In Press).
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: 3. Maryam Zand-vakili, Hussein Eimani, Afsaneh Golkar-Narenji, Poopak Eftekhari-Yazdi, Abdolhosein Shahverdi & Paul Edward Mozdziak. 2016. Histological evaluation of the effect of VEGF on auto-transplanted mouse ovaries. Animal Cells and Systems 20: 260-266
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Stern RA, Dasarathy S, Mozdziak PE. (2016, July). Ammonia elicits a different myogenic response in avian and murine myotubes. Presented at the 2016 Poultry Science Association Annual Meeting, New Orleans, LA.