Progress 07/01/15 to 06/30/20
Outputs
Target Audience:Public, private and government targets are informed of the use of antimicrobials in animals that may affect humans through contaminated water, food and fomites. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?PCR PFGE Sensitire Training Sample Collection Salmonella and STEC Enrichment Sample Cleaning DNA Isolation Nanodrop Qubit Bioinformatics using CLC Genomics Workbench Mentoring undergraduates Media preparation Teaching Manuscript writing How have the results been disseminated to communities of interest?Publication, posters and oral presentations. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Participation in international conferences aimed at providing the student experience in public speaking, attend guest speaker-seminars and participate in workshops on developing writing skill. Develop skills through experiential learning focusing on the fundamentals in research conduct, educational resources, and direction to pursue a lifelong commitment to ethically responsible research. Learn to communicate with difference audiences, take advantage of speaking opportunities in scientific meetings and involvement in teaching other graduate students. Receive training and gain experience at state/federal agencies national/international organizations that will help them to develop professionally, networking and learning new approaches.
Publications
|
Progress 07/01/18 to 06/30/19
Outputs
Target Audience:Public, private and government targets are informed of the use of antimicrobials in animals that may affect humans through contaminated water, food and fomites. Changes/Problems:Only one student is supported in this program. We have not been able to successfully recruit a 2nd student since the loss of one student due to cancer. What opportunities for training and professional development has the project provided?PCR PFGE Sensitire Training Sample Collection Salmonella and STEC Enrichment Sample Cleaning DNA Isolation Nanodrop Qubit Bioinformatics using CLC Genomics Workbench Mentoring undergraduates Media preparation Teaching Manuscript writing How have the results been disseminated to communities of interest?Posters and oral presentations. Manucript has been accepted for publication. What do you plan to do during the next reporting period to accomplish the goals?Over the past year, the student finished her thesis experiments to gauge how effective a vegetative buffer strip is at reducing the transmission of Salmonella spp. from dairy cattle and poultry operations to fresh produce. In April, she started her second year of collecting samples at the Piedmont Research Station. The final collection trip was completed in July 2019. Through October-December the student completed the challenge experiment to determine what the carrying capacity is for the vegetative buffer strip. The focus now is to process the samples collected (e.g. DNA isolation and broth microdilution) and prepare them to be sequenced using the Illumina MiSeq. Ayanna Glaize will continue her path towards completion of requirements for graduation and international work.
Impacts
What was accomplished under these goals?
Over the past year, the student finished her thesis experiments to gauge how effective a vegetative buffer strip is at reducing the transmission of Salmonella spp. from dairy cattle and poultry operations to fresh produce. In April, she started her second year of collecting samples at the Piedmont Research Station. The final collection trip was completed in July 2019. Through October-December the student completed the challenge experiment to determine what the carrying capacity is for the vegetative buffer strip. In-between collection trips the student has presented research outcomes at three local, national, and international conferences in the last year. She has continued to take a mentorship role in the lab for undergraduate students and visiting scholars. This includes training in GLP and lab techniques such DNA isolation and PCR, and the software program CLC Genomics Workbench. Beginning in Fall 2019, the student began to participate in the Preparing the Professoriate program to enhance her teaching skills and to learn what it takes to become a faculty member at a land-grant university. The student has submitted her manuscript on the transmission of non-O157 Escherichia coli on sustainable farms in TN and NC to the International Journal of Food Microbiology and has been accepted. She is now in the process of preparing for her preliminary exam.
Publications
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2019
Citation:
Ayanna Glaize, Morgan Young, Eduardo Gutierrez-Rodriguez and Siddhartha Thakur | Triangle Global Health Annual Conference | Durham, NC Oct. 2019
The Use of Vegetative Buffer Zones to Reduce the Risk of Salmonella Transmission from Animal Operations to Fresh Produce
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2019
Citation:
Ayanna Glaize, Morgan Young, Eduardo Gutierrez-Rodriguez and Siddhartha Thakur | CVM Annual Research Forum | Raleigh, NC Aug. 2019
The Use of Vegetative Buffer Zones to Reduce the Risk of Salmonella Transmission from Animal Operations to Fresh Produce
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2019
Citation:
Ayanna Glaize, Morgan Young, Eduardo Gutierrez-Rodriguez, and Siddhartha Thakur | International Association for Food Protection | Louisville, KY Jul. 2019
The Use of Riparian Buffer Zones to Reduce the Risk of Salmonella Transmission from Animal Operations to Fresh Produce
- Type:
Journal Articles
Status:
Accepted
Year Published:
2019
Citation:
Manuscript on the transmission of non-O157 Escherichia coli on sustainable farms in TN and NC accepted to the International Journal of Food Microbiology
|
Progress 07/01/17 to 06/30/18
Outputs
Target Audience:Public, private and government targets are informed of the use of antimicrobials in animals that may affect humans through contaminated water, food and fomites. Changes/Problems:We have been unsuccessful to recruit a 2nd minority student (after the loss of one student due to cancer). We are looking for additional funding to complete the 2 years of NIFA's support to recruit the 2nd student (due to the no cost extension of the current grant support). We have contacted 2 possible candidates and are evaluating their potential to complete the program. What opportunities for training and professional development has the project provided?Skills Learned: PCR PFGE Sensitire Training Sample Collection Salmonella and STEC Enrichment Sample Cleaning DNA Isolation Nanodrop Qubit Bioinformatics using CLC Genomics Workbench Mentoring undergraduates Media preparation How have the results been disseminated to communities of interest?Posters and oral presentations. Manuscript under development. What do you plan to do during the next reporting period to accomplish the goals?One student will continue her path towards completion of requirements for graduation and international work.
Impacts
What was accomplished under these goals?
Over the past year, the student started her thesis experiments to gauge how effective a riparian buffer strip is at reducing the transmission of Salmonella spp. from dairy cattle and poultry to fresh produce. In March, she started writing the S.O.P.'s for sample collection and enrichment of samples gathered at the Piedmont Research Station. Currently she hast completed the 7th collection trip and plan on doing the last collection trip of the year at the end of November. In-between collection trips, the student started learning about bioinformatics, next generation sequencing, and computer coding in preparation for laboratory work after completion of the data collection phase of her study. She has taken a mentorship role in the lab for students who join the lab. This includes training in GLP and lab techniques such DNA isolation and PCR, and the software program CLC Genomics Workbench. She has presented research outcomes at two national and at an international conferences in the last year. This work has improved her communication skills to different and present results of scientific studies. The student is completing a manuscript on shiga-toxin producing Escherichia coli's antimicrobial resistance profile and preparing the isolates for whole genome sequencing on the Illumina MiSeq. She has also performed basic bioinformatics analysis to characterize the antimicrobial resistance genotype for each isolate.
Publications
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2017
Citation:
Ayanna Glaize | CVM Annual Research Forum | Raleigh, NC
Molecular characterization of non-O157 STEC isolated from sustainable farming systems using whole genome sequencing
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2018
Citation:
Ayanna Glaize, Irene Hanning, Sandara Diaz-Sanchez, Arnoud Vanvliet, Eduardo Gutierrez, Christorpher Gunter, and Siddhartha Thakur | Triangle Global Health Annual Conference | Raleigh, NC
Molecular Characterization of Non-O157 STEC Isolated From Sustainable Farming Systems Using Whole Genome Sequencing
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2018
Citation:
Ayanna Glaize, Eduardo Gutierrez-Rodriguez, Christopher Gunter, and Siddhartha Thakur | International Association for Food Protection | Salt Lake City, UT
Molecular Characterization of Non-O157 Shiga Toxin-producing E. coli Isolated from Sustainable Farming Systems Using WGS
- Type:
Journal Articles
Status:
Other
Year Published:
2018
Citation:
Ayanna Glaize, Irene Hanning, Sandra D�az-S�nchez, Chris Gunter, Eduardo Gutierrez-Rodriguez, Arnoud Vanvliet, and Siddhartha Thakur
Molecular characterization of non-O157 SEC isolates from produce and environment sources from operating sustainable farming systems using whole genome sequencing
Manuscript being written and not submitted for publication at this time.
|
Progress 07/01/16 to 06/30/17
Outputs
Target Audience:Public, private and government targets are informed of the use of antimicrobials in animals that may affect humans through contaminated water, food and fomites. Changes/Problems:One of the students is lagging behind schedule with course work due to poor grading in one required course. Alternative plans are being evaluated to keep the student; B average is required to continue receiving funding and the student had a B- last semester. An exemption was granted by the university. What opportunities for training and professional development has the project provided?The students will participate in the "Preparing the Professoriate", a program that provides the students with teaching opportunities under the direction of faculty mentor and offers future faculty preparation experience. Students have participated in seminars at the college and other institutions, visits to industry, and rotations through different laboratories. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?1. Students will apply for the "Preparing the Professoriate" and prepare a course relevant to their interests. 2. Students will participate in teaching and mentoring of other graduate students. 3. Dissertation plans will evolve to approved projects. 4. Students will plan international experience to complement their dissertaion. 5. Students will present an abstract at a local meeting or university meeting.
Impacts
What was accomplished under these goals?
1. Students have completed basic course work and are now taking additional requirements for their focus area in population medicine including statistics and study design. 2. Students have been working on laboratory techniques that will be needed for their field work and data collection. 3. Students have met periodically with faculty in different areas of interest to determine additional needs or courses to complete their basic training. 4. PhD Committees have been designated and dissertation planning has started.
Publications
|
Progress 07/01/15 to 06/30/16
Outputs
Target Audience:Two African AmericanPhD students were recruited as explained in the proposal and will start the graduate program August 2016. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Successfully recruited two minority students, female and African American for our CBS program. The students start August 2016 and already are registering for courses.
Publications
|
|