Source: DUNN'S FISH FARMS, INC. submitted to NRP
OUT OF SEASON PRODUCTION OF LARGEMOUTH BASS FINGERLINGS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1006392
Grant No.
2015-33610-23548
Cumulative Award Amt.
$100,000.00
Proposal No.
2015-00496
Multistate No.
(N/A)
Project Start Date
Jun 1, 2015
Project End Date
Jan 31, 2017
Grant Year
2015
Program Code
[8.7]- Aquaculture
Recipient Organization
DUNN'S FISH FARMS, INC.
1106 MINNOW FARM RD
BRINKLEY,AR 72021
Performing Department
(N/A)
Non Technical Summary
Our goal is to develop a new largemouth bass fingerling production system/strategy that will employ "out-of-season" fall spawning of largemouth bass in raceways, followed by winter feed-training and the on-growing of advanced fingerlings indoors. The research outlined in this proposal includes three main objectives: Objective 1. Develop brood-stock acclimation facilities and protocols for out-of-season spawning of largemouth bass in raceways. Specifically, we need to compare spawning results of brood-stock conditioned in existing flow-through raceways to those conditioned in recirculating systems designed for this purpose. Objective 2. Determine the best method for culturing largemouth bass fingerlings during the winter months. Specifically, we need to determine if it is more cost effective to utilize existing feed-training raceways, or to develop new, recirculating systems for this purpose. Objective 3. Conduct an economic analysis (enterprise budget analysis) based on the outcomes of Objectives 1 and 2, to compare the overall economic feasibility of this new production strategy with traditional fingerling production methods. If successful, this would decrease the time and pond space required to produce fingerling bass, and would subsequently free up more pond space for the production of food-sized largemouth bass. The results generated from the proposed research are necessary to determine the overall feasibility of this novel production strategy and to determine future facilities required and quantities of brood-stock necessary to meet our production goals. Out-of-season fingerling production would significantly improve our overall farm production efficiency.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
30708101081100%
Knowledge Area
307 - Animal Management Systems;

Subject Of Investigation
0810 - Finfish;

Field Of Science
1081 - Breeding;
Goals / Objectives
Increase profitability of largemouth bass production through out of season production of fingerlings.1. Develop brood-stock acclimation facilities and protocols for out of season spawning of largemouth bass.2. Determine most profitable method to culture largemouth bass fingerlings through the winter months.3. Conduct an economic analysis (enterprise budget analysis) based on the outcomes of objectives 1 and 2 listed above to compare the overall economic feasibility of this new production strategy with traditional culture methods.
Project Methods
Develop brood-stock acclimation facilities and protocols for out-of-season spawning of largemouth bass. For this objective, we will install a recirculating aquaculture system adjacent to our existing flow-through raceway brood-stock acclimation facility. The recirculating system will consist of fiberglass tanks (10' dia, 4' depth), a micro screen drum filter, a 3 cubic meter moving bed filter with regenerative blower system for nitrogenous waste treatment and de-gassing, ultraviolet sterilizer, a 50,000 BTU heater/chiller "heat-pump" and heat exchangers for temperature control, and oxygen saturation cones. The room will be well-insulated and the lights will be controlled by simple mechanical timers for photoperiod manipulation. Dr. Jeonghwan Park, Assistant Professor, Aquaculture and Engineering at UAPB has agreed to assist with the system design and implementation.In order to compare our existing facility with the proposed recirculating facility, an equal number of brooders from the same source ponds will be transferred to each facility by mid-June of 2015. Fish in both facilities will be conditioned as closely as possible to the following protocol which was closely adapted from the out of season spawning protocol developed by Michael Mathews at the Florida Bass Conservation Center in Webster Florida (Mathews, 2013). Our manipulation plans for length of day and water temperature are concisely presented in Figure 1. From mid-June to mid-July, temperature and day length will be decreased from 85 F and 14 hour days to 50 F and 8 hour days. Fish will remain at 50 F and 8 hour days from mid-July to mid-August. Temperature and photoperiod will then be increased from mid-August through mid-September with an end target of 72 F and 14 hour days by September 15, 2015. Throughout the conditioning process, Dr. Alf Haukenes, Assistant Professor, Fish Physiology at UAPB will sample brooders for key physiological indicators that can be compared to similar data taken from brooders during the normal spawning season. Surface mucus samples will be collected from fish held in each conditioning scenario. These samples will be assayed for 11α-ketotestosterone and vitellogenin (Barkowski 2012); these two constituents are important signals that characterize the annual reproductive cycle in fishes. Differences among the treatment groups will be determined using analysis of variance followed by appropriate mean separation procedures.At the completion of the conditioning protocol, brooders from each treatment will be randomly assigned to their respective spawning raceways (Figure 4). Five spawning raceways will be dedicated to brooders acclimated in our existing facility and five spawning raceways will be dedicated to brooders acclimated in the new recirculating facility. Mats will be checked daily and any spawns will be collected and transferred to hatching troughs. The number of spawns produced by each treatment as well as the number of spawns per female in each treatment will be compared statistically with Student's T-Test.Fingerlings produced through out of season spawning will require intensive feed training by mid-late November. Our normal (proprietary) feed training protocol will be followed and typically requires 4 to 6 weeks. Because low water temperatures in December would preclude active feeding in ponds, our objective is develop protocols for growing these feed-trained fingerlings indoors through February with a target pond stocking date of March 1, 2015. Previous farm experience indicates that fingerlings can be successfully overwintered in our existing feed-training raceway system. However, since this is a flow-through system utilizing 64 F well water, feeding activity is somewhat suppressed. We are proposing to investigate the utility of using the same recirculating aquaculture system used for fall brood-stock conditioning to rear fingerlings through the winter months. This system would allow fingerlings to be cultured at 78 F. Growth, survival, food conversion ratio, and growth rates of fingerlings reared in the recirculating aquaculture system will be compared to those fingerlings reared in our existing flow-through raceway system.In order to perform the successful winter culture of largemouth fingerlings in the indoor systems, optimum stocking densities need to be determined prior to farm-wide application. Thus, we will cooperate with the UAPB research team (Drs. Park and Roy) to evaluate the effects of stocking densities on survival, growth, and size dispersal in an experimental closed indoor system. The experiment will be conducted in the UAPB Aquaculture Research Station. Feed-trained and well-graded fingerlings (approximately 2 inches long) will be obtained from Dunn's Fish Farms Inc. and transported to the UAPB Aquaculture Research Station. An experimental recirculating system consisting of 24 rectangular plastic tanks (2 ft length x 2 ft width x 1.5 ft depth), a bubble-washed bead filter (2 ft3 bead capacity), a UV sterilizer, a down-flow oxygen contacter, and a centrifugal pump (50 gpm) will be utilized for this experiment. The experimental stocking densities will be set at 150, 300, 450, 600, 750, 900 fish per tank, with four replicate tanks per density. Water temperature will be maintained at 78 F using a submersible heater and/or a drop-down chiller. Dissolved oxygen will be maintained at a minimum of 6 mg/L over the course of the experiment. Gentle aeration will be administered in the middle of each tank for mixing water and efficient solids removal toward a center drain. Fish will be fed a formulated diet (42% protein and 16% lipid) distributed by automatic feeders. Initially, the feed ration will be set at 5% of total body weight and will be adjusted biweekly after checking the satiation level by hand feeding. The experiment will be conducted for four months. The tanks will be checked daily for fish mortality, and the mortality due to cannibalism will be identified during the course of the experiment. Fish weight and number will be measured biweekly to evaluate weight gain, survival, feed conversion, and daily growth. In addition, in order to observe size variation based on the stocking densities, individual weight and body length will be measured every four weeks.We will also conduct an economic analysis (enterprise budget analysis) based on the outcomes of Objectives 1 and 2, to compare the overall economic feasibility of this new production strategy with traditional culture methods. All production inputs and production related economic data will be collected throughout the process of completing objectives 1 and 2. Extension personnel with the UAPB Aquaculture/Fisheries Center will assist with periodic sampling and routine collection of all pertinent production and economic data. At the completion of the Objective 2 work plan, data will be summarized and comparisons will be made to farm data collected from traditional culture methods during the spring of 2016. Dr. Luke Roy and Mr. Matt Recsetar, Extension Aquaculture Specialists with the University of Arkansas at Pine Bluff, have agreed to assist with the data collection throughout the production process and Dr. Carole Engle, Professor of Aquaculture Economics will take the lead role in the economic data analysis process.

Progress 06/01/15 to 01/31/17

Outputs
Target Audience:Our target audience is the management and staff of Dunn's Fish Farms, Inc. The nature of this project is to improve the overall production efficiency of largemouth bass on our farm. The target audience reached by these efforts are management and staff of Dunn's Fish Farms, Inc. These individuals are responsible for the day to day operations of the facility and must integrate these new production techniques and protocols into existing farm operations. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Four management personnel and 16 staff members have gained a working knowledge of Recirculating Aquaculture Systems and the protocols we have established to produce largemouth bass fingerlings in the RAS. How have the results been disseminated to communities of interest?Results of the UAPB density and temperature studies have been presented to the larger aquaculture community by Dr. Jeongwan Park at the Aquaculture America 2016 meeting. A manuscript summarizing these results is in progress. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? 1. Develop brood-stock acclimation facilities and protocols for the out of season spawning of largemouth bass. The broodstock acclimation facility was completed and is fully functional. Five raceways in an existing hatchery facility were partitioned off and re-modeled into a Recirculating Aquaculture System. This system now has four functioning fish holding raceways that can either be a flow-through system with well water or tied together into a recirculating system. The fifth raceway serves as the return sump and incorporates a rotating drum filter, a moving bed bioreactor, and UV sterilization. Photoperiod can be controlled through timers and water temperature is controlled with a heat pump. Water temperature and photoperiod protocols have been developed and were proven to be effective during the fall of 2016. 2. Determine the most profitable method to culture largemouth bass fingerlings through the winter months. A 25' by 50' section of our existing hatchery facility was enclosed and retrofitted with Recirculating Aquaculture System components. The system is composed of three, 3000 gallon circular tanks, ozone injection, a microscreen drum filter, an existing raceway modified to incorporate a moving bed bioreactor, a degassing aerator, and a pumping station. Each tank is fitted with an oxygen saturation cone and automatic oxygen monitoring and control to maintain optimal oxygen levels. Temperature can be maintained with an incorporated water source heat pump. A series of fingerling growth studies were conducted in this system with our existing raceway system serving as a control. In addition to the work accomplished on site, Dr. Jeongwan Park conducted a series of experiments at the University of Arkansas at Pine Bluff to determine the optimal density and temperature for largemouth bass fingerlings produced in RAS systems. This information, and the data collected from the on-farm growth studies has helped us produce and fine tune our RAS largemouth bass fingerling production protocols. 3. Conduct an economic analysis (enterprise budget analysis) based on the outcomes of objectives 1 and 2 listed above to compare the overall economic feasibility of out of season production strategies with traditional culture methods. A detailed economic analysis was developed by Dr. Carole Engle to compare the following: 1) Largemouth bass spawning in the spring using traditional practices versus largemouth bass spawning following early acclimation of broodstock; and 2) Winter production of largemouth bass fingerlings in either existing raceways or in a Recirculating Aquaculture System. Enterprise budgeting techniques were used to develop detailed cost estimates of the scenarios for analysis following standardized methodologies (Engle 2010). The first step in the analysis was to develop a complete enterprise budget for the traditional spring spawning system for largemouth bass. The representative hatchery system developed by Stone et al. (2008) was used as the base structure for the traditional largemouth bass hatchery and spawning analysis. This detailed analysis found that the cost to produce 1 million fry (the standardized unit of measurement for the analysis) to be $3,499 for traditional spawning in the spring. Costs to construct the temperature acclimation room to acclimate largemouth bass broodstock for early spawning in the spring were outlined. Necessary equipment included: drum filter, water pumps, heat pump, biofilter, UV sterilizer, demand feeders, and the raceways themselves to hold the broodstock for temperature acclimation. The total investment cost for the acclimation room was $15,420, with annual depreciation of $1,221. Spawning trials in this project resulted in greater numbers of spawns per female brooder in the early acclimation. Thus, fewer females and fewer raceways were needed to produce 1,000,000 fry (the standardized unit of measurement for the analysis) due to the greater number of spawns obtained per female with early acclimation. Broodstock costs per million fry produced were lower because fewer broodstock were needed due to the greater number of spawns per female. Costs to produce 1 million fry from early acclimated broodstock were $1,883. Early acclimation of largemouth bass broodstock resulted in a decreased cost of production of largemouth bass of $1,616 per million fry, a decrease in production cost of 46%. Another detailed analysis was conducted to compare the cost of producing fingerlings through the winter months. Production data from the trials conducted in this study were used, including the weight of fingerlings produced per raceway. Total costs were $3.68 per lb of fingerlings produced in traditional raceways over the winter. The total investment cost for the RAS system was $102,609, with an annual depreciation of $8,280 and an average interest on the investment of $5,131 (accounting for the opportunity cost of the capital used). The quantity of feed fed and the total weight of largemouth bass fingerlings produced from the fingerling production trials conducted in this study were used. The cost to produce largemouth bass fingerlings in a Recirculating Aquaculture System over the winter was $2.30/lb of fingerling produced. The winter production cost of largemouth bass fingerlings in a Recirculating Aquaculture System was $1.38/lb less than the cost of winter production of largemouth bass fingerlings in raceways, a decreased cost of 37.5%. In summary, the cost of winter production of largemouth bass fingerlings was substantially lower in the Recirculating Aquaculture System than in raceways. The cost reduction identified in this study was due primarily to the greater growth rate of fingerlings in the Recirculating Aquaculture System as compared to the growth rate of largemouth bass fingerlings in the raceways. In the Recirculating Aquaculture System, largemouth bass fingerlings grew to 0.0423 lb as compared to a final weight of only 0.012 lb in the raceways, with similar survival. The subsequent greater total weight of fingerlings produced spread the fixed costs associated with the capital investment in tanks and filters across a greater volume of production and resulted in a substantially lower cost per pound of fingerlings produced in the Recirculating Aquaculture System. References Engle, C.R. 2010. Aquaculture Economics and Financing: Management and Analysis. Blackwell Scientific, Ames, Iowa. Engle, C.R. and B. Southworth. 2013. Costs of raising largemouth bass fingerlings. ETB 260, Cooperative Extension Service, University of Arkansas at Pine Bluff, Pine Bluff, Arkansas. Stone, N., C.R. Engle, and E. Park. 2008. Production enterprise budget for golden shiners. Southern Regional Aquaculture Center Publication No. 122, Southern Regional Aquaculture Center, Mississippi State, Mississippi.

Publications


    Progress 06/01/15 to 05/31/16

    Outputs
    Target Audience:Our target audience is the management and staff of Dunn's Fish Farms, Inc. The nature of this project is to improve our farms overall product efficiency and the managemen and staff of Dunn's Fish Farms, Inc. are the key individuals who must integrate these new production techniques into existing fish production protocols. Changes/Problems:We have no major changes or problems to report. Physically constructing the acclimation facility took longer than expected and this pushed our spawning window too far into the fall. We will be able to initiate our acclimation protocols on time in this next attempt in the fall of 2016 and believe that we will have more reasonable production numbers on which to base an economic analysis. What opportunities for training and professional development has the project provided?Four management personnel and 16 staff members have gained a working knowlege of recirculating aquaculture systems and associated water quality parameters. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?We now have the facility and protocols in place to attempt another out of season spawn. Last years spawn was rushed due to the time constraints of physically building the facility. While we did produce fry, we were not able to optimize survival to fingerlings due to the onset of cold weather. In this reporting period, we will have an opportunity to optimize our protocols and timing to better accomplish our goal.

    Impacts
    What was accomplished under these goals? 1. Develop brood-stock acclimation facilities and protocols for out of season spawning of largemouth bass. The brood-stock acclimation facility has been completed and the acclimation protocols developed proved to be at least marginally effective. Additional work will be required to further improve acclimation protocols to improve spawning percentages and fry survival. 2. Determine most profitable method to culture largemouth bass fingerlings through the winter months. Production and growth data were collected throughout the winter of 2015-2016. More data will need to be collected in the fall of 2016 in order to complete this objective. 3. Conduct an economic analysis (enterprise budget analysis) based on the outcomes of objectives 1 and 2 listed above to compare the overall economic feasibility of this new production strategy with traditional culture methods. The enterprise budget framework is currently under construction and we will be ready to plug in our production numbers as soon as we finish the next round of spawning this fall.

    Publications