Source: UNIVERSITY OF CALIFORNIA, DAVIS submitted to NRP
⿿BACKYARD POULTRY AS A RESERVOIR FOR RESPIRATORY DISEASES IN COMMERCIAL POULTRY AND VICE VERSA?⿝
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1005917
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Feb 19, 2015
Project End Date
Sep 30, 2016
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF CALIFORNIA, DAVIS
410 MRAK HALL
DAVIS,CA 95616-8671
Performing Department
Population Health & Reproduction
Non Technical Summary
Urban poultry are becoming more and more common. While specific numbers are not available the number of backyard (BY) poultry submitted to the California Animal Health and Food Safety Laboratory (CAHFS) for diagnosis have increased by 383% over the last 6 years (10). Lack of appropriate biosecurity measures, inconsistent vaccination, long lifetime span, and poor access to veterinary care are common denominators with respect to BY chickens. The combination of all these factors makes them vulnerable to several diseases. Consequently, BY poultry can be both sentinels and amplifiers of several diseases including "exotic diseases." An example of the role of BY flocks (BYF) in disease transmission and amplification is the 2002 exotic Newcastle disease (END) outbreak affecting the commercial poultry industry in California that was originated in backyard poultry flocks (3, 6, 9). According to the END Task Force created after this event the cost of eradication alone was $176 million dollars. This was not the first time in which a foreign animal disease spread from BY poultry farms causing substantial economic consequences (1, 8, 9). BYF were also responsible for the 1998 END in the California central valley, 2000 END outbreak in Italy, and 2006 H5N1 highly pathogenic avian Influenza in Asia (2, 3). Seroprevalence studies are crucial to assess the exposure of poultry flocks to different pathogens, in addition helps detecting their potential as reservoirs of disease. This strategy has been used to identify disease risk in BYF (4, 5). Respiratory pathogens are one the most common cause of disease in commercial and non-commercial poultry operations. These events are not only restricted to respiratory signs but also reproductive, urogenital and production losses restricting the potential and wellbeing of the flock ending, in some cases, in mortality. Respiratory pathogen seroprevalence information in small BYF is limited. A retrospective serologic analysis of backyard poultry submissions from October 2010 to October 2012 to CAHFS laboratory in Turlock, suggests there is a significant challenge and exposure to Mycoplasma gallisepticum (MG) and infectious bronchitis virus (IBV). The following table displays the results of the serologic analysis and provides supporting evidence that backyard flocks are a potential nidus for a disease outbreak in the California central valley, home for several hundred commercial poultry facilities.Table 1: Prevalence of respiratory diseases diagnosed in BYF submissions to the CAHFS laboratory Turlock branch from October 2010 to October 2012.Infectious DiseaseSample size (n=)Percent PositiveNewcastle Disease1257 %Infectious Bronchitis12536 %Mycoplasma gallisepticum6075%Mycoplasma synoviae4842%The knowledge of backyard respiratory disease seroprevalence in areas distant and close to commercial poultry production will help to create a seroprevalence database that can be used to assess risk with respect to the transmission from BYF to the commercial poultry industry and establish preventive/education measures.This proposal considers high priority issues listed by CFAH such as disease control and surveillance, emerging diseases and accurate diagnosis.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
31132991170100%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3299 - Poultry, general/other;

Field Of Science
1170 - Epidemiology;
Goals / Objectives
-Determine biosecurity, management and seroprevalence information related to respiratory diseases in BYF close to commercial poultry premises-Determine biosecurity, management, and seroprevalence information related to respiratory diseases in BYF distant to commercial poultry premises-Compare results and establish the potential risk that BYF pose to the introduction of respiratory diseases to commercial poultry industry
Project Methods
Step 1. Identify backyard flocks close to commercial poultry premises (1 mile radius distance), which has been shown to be a disease risk factor (7)and distant from poultry commercial premises preferentially in a residential area (Davis). This will be done with the help of the extension poultry veterinarian and animal science poultry extension specialist, backyard flock associations and using the data already collected by CDFA and CAHFS based on submissions. Producers and/or poultry enthusiasts will be contacted and requested to participate.Step 2. A visit and a short survey will be performed in order to assess breeds, flock health, prevalence of respiratory problems, mortality, vaccination status, and biosecurity. Blood samples will be collected, serum will be isolated.Step 3. Using commercial ELISA kits, antibodies against some of the most important respiratory pathogens in poultry such as: avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS) and Ornithobacterium Rinotracheale (ORT) will be assessed. In addition, flocks will be mapped and conclusions will be drawn based on proximity to commercial poultry premises. We anticipate that our results will support our research hypothesis.Data analysis: Descriptive statistics will be used to compare and express seroprevalence in the different BYF. Prevalence of the different diseases will be calculated and mapped. Results from the different locations will be compared between each other. Finally ArcGIS will be used to map locations and identify spatial and temporal associations.Our poultry extension specialist will be fully involved in the detection of the sampled farms, sample collection, health assessment of the flocks, data analysis and knowledge dissemination/education based on the results of this investigation.

Progress 10/01/15 to 09/30/16

Outputs
Target Audience:Our target audience is small poultry flock owners, commercial poultry industry, small and preventative veterinary medicine veterinarians Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project has given the opportunity to train on Master of science student in poultry medicine and epidemiology How have the results been disseminated to communities of interest?Preliminary results have been disseminated to: Small flock owners Comercial poultry industry By outreach talks inside California What do you plan to do during the next reporting period to accomplish the goals?In order to accomplish goals: We will target sampling of premises close to commercial poultry We will assess risk of getting this respiratory diseases for commercial poultry based on our results We will continue updating and presenting our data to the interested parties

Impacts
What was accomplished under these goals? We have worked on the first two goals of this project. We are still in the process of sampling in order to get our target number of sampled chickens and premises. After having the targete numbers (1,000 birds) we will start the comparison of premises close and far to poultry commercial premises. So far we have: Determined seroprevalence of the most common respiratory diseases in more than 500 birds from aproximately 100 premises. Most of this premises are far from commercial poultry We are currently working on sampling premises far from commercial poultry

Publications

  • Type: Theses/Dissertations Status: Other Year Published: 2017 Citation: Derksen, T., M. Pitesky, R.A. Gallardo. Backyard Flocks as a Reservoir for Poultry Respiratory Diseases
  • Type: Journal Articles Status: Other Year Published: 2017 Citation: Derksen, T., M. Pitesky, R.A. Gallardo. Backyard Flocks as a Reservoir for Poultry Respiratory Diseases


Progress 02/19/15 to 09/30/16

Outputs
Target Audience:-Small scale poultry producers (backyard, 4H and small flocks semi commercial) -Poultry veterinarians -Commercial poultry companies -Veterinarians Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?One undergraduate student was trained in poultry handling, management, sampling, ELISA testing and general lab work One Masters student was graduated as a result of this project How have the results been disseminated to communities of interest?Two small flock seminars One quality assurance seminar for poultry producers One international meeting talk One poultry diseases comnference talk One manuscript submitted for publication What do you plan to do during the next reporting period to accomplish the goals?Goals were accomplished

Impacts
What was accomplished under these goals? Raising backyard chickens is an ever-growing hobby in the United States. These flocks can be a substrate for respiratory disease amplification and transmission to commercial facilities. Five hundred fifty-four chickens from 41 backyard flocks were sampled in this study. ELISA kits were used to detect antibodies against avian influenza (AI), infectious Laryngotracheitis (ILT), Newcastle disease (ND), infectious bronchitis (IB), Ornithobacterium rhinotracheale (ORT), Mycoplasma gallisepticum (MG), and Mycoplasma synoviae (MS). All visited flock owners answered a biosecurity questionnaire that assessed biosecurity measures. The questionnaire revealed that backyard poultry owners lack simple biosecurity measures such as use of dedicated shoes, their chicken sources are unreliable and few of them benefit from veterinary oversight. Only one flock had a clear vaccination history against ND and IB. ORT, ND, IB, MS, MG and ILT were the most seroprevalent in backyard poultry flocks with 97% (41/42), 77.5% (31/40), 75% (30/40), 73% (31/42), 69% (29/42), and 45% (19/42), respectively. The vaccinated flock was not considered in these calculations. When examining the distance between backyard flocks and the nearest commercial poultry facility ND and MG were significantly more likely to be found in backyard flocks close (<4 miles) while ORT was significantly more likely in backyard chickens located far (>4 miles) from commercial poultry facilities. Birds purchased directly from NPIP hatcheries showed a reduced ND, MG, and MS antibody prevalence. Wearing dedicated shoes decreased MS antibody positive birds. Finally, history of wild bird contact had a clear effect on an increased seroprevalence of NDV and MG. This research shows the continued need to examine backyard poultry flocks and educate owners on practical management and biosecurity. Serological results suggest that backyard poultry flocks have the potential to serve as a reservoir or amplifier for poultry respiratory diseases.

Publications

  • Type: Journal Articles Status: Submitted Year Published: 2017 Citation: -Derksen T.J., Lampron R., Hauck R., M. Pitesky, R. A. Gallardo. Biosecurity Assessment and Seroprevalence of Respiratory Diseases in Backyard Poultry Flocks Located Close and Far from Commercial Premises. Avian Diseases. 2017. (Submitted).


Progress 02/19/15 to 09/30/15

Outputs
Target Audience:The main audience are backyard flock owners, commercial poultry professionals, federal and state agencies, universities and veterinarians. Little work has been done in this area so the detection of the exposure of backyar flocks to poultry pathogens is of interest to all players in the poultry bussiness from small hobby to commercial. Changes/Problems:This endevor has been more difficult to plan than what we thought. We feel confident that we are covering all the areas before performin the sampling What opportunities for training and professional development has the project provided?This project is training one master's student and has been proformed in collaboration with one post doc How have the results been disseminated to communities of interest?Results haven't been obtained yet. We have been preparing logistically and gathering/buying the tools needed to perform the experiment What do you plan to do during the next reporting period to accomplish the goals?-Analyze data of the BYF presence in the state of California -Detect the flocks that we will survey for biosecurity and sample for seroprevalence study, close and far from commercial poultry premises. -Perform laboratory analysis of the samples and analysis of the resukts of biosecurity assessments -Correlate results and write a manuscript to be published in a poultry journal -Results will also be presented in extension meetings and poultry meetings

Impacts
What was accomplished under these goals? We have been working on the biosecurity assessment questionaires for BYF distant and close to commercial premises, those will be distributed before the end of the year.

Publications

  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: "Poultry health talk" at the Heirloom festival, Santa Rosa CA This will be an approach to small flock owners to get the samples needed for the experiment