Source: UNIVERSITY OF CALIFORNIA, RIVERSIDE submitted to NRP
STRENGTHENING GRANT: PROMOTING RESEARCH IN PLANT-HERBIVORE INTERACTIONS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1005907
Grant No.
2015-67014-23283
Cumulative Award Amt.
$49,749.00
Proposal No.
2014-05301
Multistate No.
(N/A)
Project Start Date
Apr 1, 2015
Project End Date
Mar 31, 2016
Grant Year
2015
Program Code
[A1111]- Plant Health and Production and Plant Products: Insects and Nematodes
Recipient Organization
UNIVERSITY OF CALIFORNIA, RIVERSIDE
(N/A)
RIVERSIDE,CA 92521
Performing Department
College of Nat & Agr Sciences
Non Technical Summary
This Strengthening Equipment Grant requests the purchase of a large-capacity, high-light, energy-efficient growth chamber to promote research and education in the area of plant-insect interactions. The chamber will replace an antiquated growth chamber (38-yr old) that was recently retired due to fire damage. The growth chamber will significantly enhance the infrastructure of the Botany and Plant Sciences Department at the University of California at Riverside (UCR) and the College of Natural and Agricultural Sciences by increasing reliable growth space and energy efficiency. This chamber will primarily be used to accelerate two newly emerging research programs. In the future, the chamber will provide flexibility for use by other faculty with interests in plant-insect research at the UCR. The proposed chamber has a dual configuration allowing for growth of larger plants (60") or a two-tiered configuration that will maximize plant capacity. The chamber will be housed in a new plant growth facility in close proximity to the department's faculty. The College and Research and Development Office will provide matching funds for the grant. UCR is eligible for a Strengthening Grant based the two criteria outlined in Part VIII H of the RFA. First, UCR is a degree-granting "minority-serving institution". UCR's total minority enrollment exceeds 74% with 6.6% African American, 35.7% Asian American, 31.5% Chicano and Latino, and 0.5% Native American students (http://www.ucr.edu/about/facts.html). Second, UCR is "not among the most successful universities and colleges for receiving Federal funds for science and engineering research."
Animal Health Component
(N/A)
Research Effort Categories
Basic
100%
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2111640113050%
2111460104050%
Goals / Objectives
This Strengthening Grant will provide funds for the acquisition of a plant growth chamber for insect-plant interactions studies. The chamber is impact several a new and an established research initiatives on plant-insect interactions. Healthy, insect-free plants are critical for research on plant resistance genes and their mechanisms of action. Plants grown under controlled conditions are used both to maintain pure insect colonies and eliminate uncontrolled variables in experiments. The first project is new, has received favorable reviews at the USDA but was not funded, and proposes to identify and deploy whitefly resistance loci in alfalfa (Medicago sativa). The second project, which is funded, investigates the role of tomato's leucine aminopeptidase in the control of resistance traits that deter lepidopteran larval growth and development. Investment in UCR's infrastructure will promote research excellence and productivity for plant-insect interaction research primarily in the Walling lab, as well as other current and future faculty in this area.
Project Methods
The Strengthening Grant is for a peice of equipment so a description of the methods does not seem appropriate for this project. The chambers are used to grow plants for wounding time courses and whitefly infestations. In general, we examine changes in the transcriptome and proteome.

Progress 04/01/15 to 03/31/16

Outputs
Target Audience:Our grant allowed the purchase of a new plant growth chamber. The chamber has been functional for less than 2 months. The chamber is impacting a large number of projects involving plant-insect interactions. There was no real target audience for this period (other than nagging UCR Physical Plant to hurry up installation steps!) Changes/Problems:We were delayed in getting the chamber ordered due to the construction of a new facilities to house the chamber. When the decision was finally made by our campus, I ordered a water-cooled chamber. It took over 5 months for construction. It was installed in December 2015 and has been functional since that time. The chamber is in a temporary location (Fawsett Hall) and will be moved to a new facility that will break ground shortly. The home for the chamber is not pretty, but we are definitely functional! What opportunities for training and professional development has the project provided?The chamber has already supported the research of three graduate students and one high school volunteer. My student Patrick has learned to be an excellent research mentor. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? The growth chamber has only been active for three months. We have learned how to program the chamber and its energy efficient LED lights. We had one experiment impacting the LAP-insect interaction project be completed. We have evidence that LAP impacts volatile production in tomato. We are using LAPpromoter:GUS lines to look for LAP expression in the trichomes. We have just put up four Arabidopsis experiments in the chamber. (1) My Grad Student Danielle Garceau is setting up a JA and SA treatment timecoure. Similar timecourses are being done in tomato, cassava and alfalfa. Based on the timing of key sentinel genes, we will choose two timepoints to compare the JA and SA networks (via RNA-seq) in the model plants tomato and Arabidopsis (3 week old) and non-model plants (Cassava and alfalfa). (2) This project is enabled by my graduate student Patrick Thomas. We are growing seed for Arabidopsis Col-0 and three sweet mutants (sweet11, sweet12, and sweet11 sweet12). A high school student (Esther Koh) working in my lab has tentative data suggesting that the sweet mutants disrupt a sucrose gradient that is critical for whiteflies finding the phloem-seive element. Esther came in first place in the Riverside city high school competitions and in first place in the regional Science Fair. She one a Center for Plant Cell Biology Center award at the regional Science Fair. She moves on to the State competition in May 2016. (3) We are growing plants for Dr. Xuemei Chen (Dept Botany and Plant Sciences). She needed a pathogen and pest free environment to test for the role of small RNAs in plant defense. (4) We are testing the staining of DAP1:GUS and DAP2:GUS plants during development. This will complete a manuscript that will be submitted in late spring 2016. After the short time dedicated to Arabidopsis, the chamber will be dedicated to my alfalfa-whitefly project. A timecourse for collection of whitefly infested materials is needed to understand the changes in gene expression in whitefly-resistant and -susceptible alfalfa.

Publications