Source: UNIVERSITY OF CALIFORNIA AT SANTA CRUZ submitted to NRP
ASSESSING THE IMPACT OF AGRICULTURAL PRACTICES ON PHOSPHOROUS AVAILABILITY AND LOSS USING OXYGEN ISOTOPES OF PHOSPHATE IN SOIL
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1004793
Grant No.
2013-67019-21387
Cumulative Award Amt.
$487,500.00
Proposal No.
2013-02817
Multistate No.
(N/A)
Project Start Date
Sep 1, 2013
Project End Date
Aug 31, 2018
Grant Year
2013
Program Code
[A1401]- Foundational Program: Soil Health
Recipient Organization
UNIVERSITY OF CALIFORNIA AT SANTA CRUZ
1156 HIGH STREET
SANTA CRUZ,CA 950641077
Performing Department
(N/A)
Non Technical Summary
Phosphorus (P), an essential element for growth and development, is taken up by plants as phosphate (Pi), however Pi is unevenly distributed and relatively immobile in soils. As a result, more than 30% of the world's arable land requires the application of P fertilizers for cropping and many natural ecosystems are P limited. Unfortunately, part of the applied P in intensive cropping systems can enter the waterways through runoff and erosion, contributing to pollution and eutrophication of surrounding groundwater, lakes and coastal environments. Tracking P cycling, mobility in soils, and determining soils' P availability to plants is challenging because adsorption/desorption, immobilization (occlusion by or precipitation as minerals), mineralization (conversion of organic P compounds to Pi), and uptake (by organisms) all occur simultaneously in the soil. Determining soil P availability and mobility and how these characteristics vary with soil type and agriculture practices will help reduce P loss from agriculture systems and contribute fundamental understanding to inform science based management plans.A clear understanding of all aspects of soil P biogeochemistry is required to determine P availability and mobility in soils, but analytical techniques have not been available to fully characterize P cycling in soils. We developed a natural isotopic tracing procedure of oxygen in phosphate to (d18Op) to track P sources and biological cycling in soils. We propose to apply this procedure to study P cycling in various agriculture soils representing an array of farm management practices. Work will take place at the Russell Ranch Experimental LTRAS, a long-term comparison of 10 conventional, organic and alternative cropping systems, both irrigated and non-irrigated is taking place.We expect that different processes will dominate P cycling in different soil types (soil pH, grain size, mineralogy, organic matter content, etc.) and under different management and farming systems (no tillage, and use of organic--plant residue and/or animal waste--vs. inorganic fertilizers). These processes will ultimately determine the availability of P to plants and the potential for P loss from the system.
Animal Health Component
20%
Research Effort Categories
Basic
70%
Applied
20%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
10201101000100%
Goals / Objectives
The major goal of this project is to use stable isotopes of oxygen in soil phosphate (d18Op) to determine the impacts of different farming and management practices on P cycling in soils and P availability to crops. This will be done using plots from the Russell Ranch Sustainable Agriculture Facility thus elucidating how P availability, loss and transformations relate to soil characteristics and farming practices and provide a foundation for making science based management plans that would help achieve sustainable production of ecosystem services.
Project Methods
In this research, we intend to use innovative state-of-the-art techniques combined with traditional methods to study the fate of P in soils representing different farming practices. To quantify the P fractions and distinguish between WSP, labile P (slightly bound), strongly adsorbed and precipitated P, which are important both for agronomic management and environmental protection we will use sequential P extraction methods, (e.g., Hedley et al., 1982; Tiessen and Moir, 1993). To shed light on the biogeochemical processes that control the transformation, movement, and storage of P in soils we will employ d18Op analyses to the different soil fractions. This information will be related to farming practices and along with ancillary data on soil properties will contribute to the construction of a unifying soil P biogeochemical cycling model under different farming practices.Soil P cycling will be investigated using d18Op associated with various soils fractions in samples from well studied plots from the Russell Ranch Sustainable Agriculture Facility (LTRAS) representing different long term farming practices including different crop rotations, irrigation and nitrogen application (Figure 3). Not illustrated in Figure 3 are the three tomato rotations (organic, conventional and the "mixed" systems) which all had different P inputs. The "mixed" has been managed as a hybrid of conventional and organic. The conventional has 45 lbs P in pre-plant fertilizer applied per year, while the "mixed" has had 45 lbs P applied every other year. The organic receives phosphorus from chicken manure compost and has an average 87 lbs P/yr applied. In addition, from 2003-2007 treatments with conventional vs. reduced till took place. Soil from the 16 different treatments (8 treatment combinations applied to two crop types) will be sampled twice during the first year of the project before seeding and during harvest. In addition the fertilizer and manure used, drainage water and crop residue will be sampled. In the second and third years, in collaboration with LTRAS personnel, different types of P additions will be applied in micro-plots within each main plot and these mirco-plots and the main plots will both be sampled during harvest season. In year 2 we will apply manure and conventional inorganic fertilizer and in year three compost and organic conventional fertilizer. Depending on results we may also test different types of manure (chicken, horse, etc.) and/or different amounts of P additions. In total the samples from the mini-plots will add 64 distinct samples in years two and three.Farming practices induce alterations in physical and chemical soil properties leading to differences in distribution of minerals and organic compounds in soil. Different soil fractions contribute differently to cycling and availability of P in soil (O'Hara et al., 2006; Solomon and Lehmann, 2000). P source used for fertilization, amount of P applied, application timing, crop type, irrigation, cultivation, and harvesting processes were all shown to influence concentrations of both Po and Pi and their association and distribution in soils (Syers et al., 2008; Solomon and Lehmann, 2000). Combining the modified Hadley chemical P fractionation and the stable isotope tracing procedures will yield important insights regarding cycling and transformations of P in soil and how they relate to farming practices.Ancillary data including soil quality measures, soil temperature, moisture and d18O of soil water, organic C content, metals associated with P in soil (Al, Fe, Ca), microbial biomass and P-enzyme activities will also be collected. Additional data from these well studied sites(such as soil type, mineralogy, texture, physical properties, organic matter content, C and N, pH, solute composition, nematode counts, etc.) will be assembled from the LTRAS data set and used to assist in interpretation. All of the above data will be used to construct a unifying model to explain P cycling is soils as they relate to soil properties and farming practices and to delineate the transformation, bioavaiability and various physical, biological and farming impacts on the P cycle. More details on the LTRAS could be found on the Russell Ranch Sustainable Agriculture Facility web page (http://ltras.ucdavis.edu/)

Progress 09/01/13 to 08/31/18

Outputs
Target Audience:Scientists in the field Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?An undergraduate student and a graduate student were supported under this project How have the results been disseminated to communities of interest?Several presentations were given in conferences by participants. One manuscript has been published and two more are being prepared for publication. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? The concentration of P in 4 distinct pools was determined in 4 different agriculture management conditions for two distinct sampling years. In addition the oxygen isotopic composition of these pools was determined.

Publications

  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Mellet, T., Selvin, C., Defforey, D., Roberts, K., Lecher, A.L., Dennis, K., Gutknecht, J., Field, C., A. Paytan (2017). Assessing Cumulative Effects of Climate Change Manipulations on Phosphorus Limitation in a Californian Grassland. Environmental Science and Technology. DOI: 10.1021/acs.est.7b04362.


Progress 09/01/13 to 08/31/14

Outputs
Target Audience:UCSC is a minority serving institute and the target audiance are racial and ethnic minorities who are socially, economically, or educationally disadvantaged. Specifically several minority undergraduate students participated and outreach activities targeted under served communities in central California Changes/Problems:Due to method development problems related to low concentrations of P in the samples it took a longer time to actually get any isotope data. In addition our mass spectrometer needed repair which resulted in further delays but things are back on track What opportunities for training and professional development has the project provided?Several undergraduate and graduate students in the 3 campuses participated in the work and got training. The hands on activity kit was used in several schools in the region as well as in the Expanding your Horizon and SACCNAS meetings How have the results been disseminated to communities of interest?Under represented students learned about the P cycle through participating in outreach activities What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period we will finish the isotope analyses, interpret the data and look for temporal trends in the isotope ratios

Impacts
What was accomplished under these goals? Determining soil P availability and mobility and how these characteristics vary with soil type and agriculture practices will help reduce P loss from agriculture systems and contribute fundamental understanding to inform science based management plans. However, tracking P cycling, mobility in soils, and determining soils' P availability to plants is challenging because adsorption/desorption, immobilization (occlusion by or precipitation as minerals), mineralization (conversion of organic P compounds to Pi), and uptake (by organisms) all occur simultaneously in the soil. A clear understanding of all aspects of soil P biogeochemistry is required to determine P availability and mobility in soils, but analytical techniques have not been available to fully characterize P cycling in soils. This project will test a newly developed isotope system to shed light on soil P cycling under different management practices. We utilize the oxygen isotopic composition of phosphate (d18Op) which is associated with various pools of P in soil (soil solution, loosely adsorbed, Fe and Al associated, and Ca associated) to elucidate some of the P transformations that take place within different soil types and under different agriculture management practices (no tillage, standard tillage, organic or mineral fertilizers etc. in a factorial design). Specifically, we will investigate how adsorption/desorption, mineralization, uptake, and immobilization processes differ between different soils and under different management and link these characteristics to P availability and mobility. Work takes place at the Russell Ranch Experimental LTRAS, a long-term comparison of 10 conventional, organic and alternative cropping systems, both irrigated and non-irrigated. Four distinct treatments are compared and the changes in d18Op among treatments and within each treatment plot with depth and over time are compared. We found that the SRP concentrations decrease with depth for all treatments but that plots receiving organic treatments have higher SRP concentrations than those treated with mineral fertilizer. The very labile phosphate (extracted by water and bicarbonate) concentrations in these soils is very low and higher concentrations are seen in the less labile fraction (NaOH and HCl extractions). Soils receiving organic fertilizer have higher SRP and TP concentrations for all fractions in the upper 60cm of the soil core, while at depth (200m) all fractions and treatments converge to lower values. Isotope data suggest that the latter more refractory fractions however are also bioavailable to crops but on longer time scales. The combined approach of determining P association with different soil fractions and ?18Op shed more light on P availability and cycling in soils. Determining soil P availability and mobility and how these characteristics vary with soil type and agriculture practices will help reduce P loss from agriculture systems and contribute towards science based management plans. Sustainable P management is critical for reducing environmental P pollution (i.e. reducing surface and groundwater pollution and risks of eutrophication). In addition, with impending shortages in fertilizer P availability in the world, data and understanding derived from this work will play critical role in our understanding of how different soil, as well as human variables affect not just total stocks but also availability of P for critical crops and vegetables.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: Phosphorus Cycling in Soils: Assessing the Impact of Agricultural Practices on Phosphorous Availability and Loss Using Oxygen Isotopes of Phosphate. CC-RISE 2015 REU student final presentation