Progress 09/01/14 to 08/31/17
Outputs
Target Audience:· Microbiologists · Veterinarians · Fish health research community · Catfish producers -Efforts Science-based knowledge has been delivered in laboratory teaching, fish health-related courses, and workshops. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project has provided training and professional development opportunities for one postdoc, and four doctoral students. How have the results been disseminated to communities of interest?Results have been presented at scientific conferences and published in peer-reviewed journals. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
-Goal one: Determine virulence and efficacy of E. ictaluri mutants in catfish (completed 100%, and reported in Year 2016). · Nothing to report -Goal two: Determine the best vaccination regime using the most effective vaccine candidate (completed 100%). · Safety and efficacy of the vaccine candidates were determined in catfish fry. · Immersion-oral vaccination experiments were conducted in outside earthen ponds. · Pathologic examinations were completed. 2) Specific objectives met -Goal one · Nothing to report -Goal two · Best oral vaccination dose was determined · Tissue persistence of best vaccine candidates was determined. · Pathologic effects of vaccine candidates were determined. · Immunological assessments of vaccine candidates were conducted. 3) Significant Results Achieved · Two of the mutants had superior efficacy in catfish fry. · Fry at three weeks or older developed better immune responses. · Immersion vaccination and oral boosting provided the best protection in catfish fry · Vaccine candidates caused little pathologic changes in tissues. · Vaccine candidates could colonize catfish but not persist a long time. 4) Key outcomes or other accomplishments realized · Two potential vaccine candidates for catfish fingerlings and fry were determined. · Protection of vaccine candidates increased when fry was three weeks or older. · Immersion vaccination and oral boosting by vaccinated feed provided the most effective protection in catfish fry.
Publications
- Type:
Journal Articles
Status:
Other
Year Published:
2017
Citation:
Kalindamar S, Lu J, Abdelhamed H, Tekedar HC, Lawrence ML, Karsi A*. 2017. Transposon mutagenesis and identification of mutated genes in growth-delayed Edwardsiella ictaluri colonies. BMC Microbiology, In preparation.
- Type:
Journal Articles
Status:
Under Review
Year Published:
2017
Citation:
Kordon A, Abdelhamed H, Ahmed H, Park J, Karsi A, Pinchuk L. 2017. Live attenuated vaccine-induced sera promote phagocytic uptake and bacterial killing in channel catfish peritoneal macrophages. Frontiers in Microbiology. Under review.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2017
Citation:
Abdelhamed H, Lu J, Lawrence ML, Karsi A*. 2017. Construction and vaccine potential of a live attenuated Edwardsiella ictaluri TonB mutant against enteric septicemia of catfish. Frontiers in Physiology, Accepted.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Abdelhamed H, Ibrahim I, Baumgartner W, Lawrence ML, Karsi A*. 2017. The virulence and immune protection of Edwardsiella ictaluri HemR mutants in catfish. Fish and Shellfish Immunology. 2017 Oct 31;72:153-160. doi: 10.1016/j.fsi.2017.10.041. PMID: 29097322
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Nho S, Abdelhamed H, Karsi A, Lawrence ML. 2017. Improving safety of a live attenuated Edwardsiella ictaluri vaccine against enteric septicemia of catfish and evaluation of efficacy. Veterinary Microbiology. 2017 Oct;210:83-90. doi: 10.1016/j.vetmic.2017.09.004. Epub 2017 Sep 14. PMID: 29103702
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Lu J, Dahal N, Lawrence M, Karsi A. 2017. Safety and efficacy of 11 Edwardsiella ictaluri in-frame deletion mutants. American Fisheries Society Fish Health Section Annual Meeting, East Lansing, MI, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Abdelhamed H, Nho S, Karsi A, Lawrence ML. 2017. Development of live attenuated Edwardsiella ictaluri vaccine against enteric septicemia of catfish. American Fisheries Society Fish Health Section Annual Meeting, East Lansing, MI, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Abdelhamed H, Lawrence ML, Karsi A. 2017. A novel vaccine candidate protects catfish against enteric septicemia. American Fisheries Society Fish Health Section Annual Meeting, East Lansing, MI, USA.
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Progress 09/01/15 to 08/31/16
Outputs
Target Audience:-Target Audiences Fish health research community Veterinarians Catfish producers Microbiologists -Efforts Science-based knowledge has been delivered in laboratory teaching, fish health related courses, and workshops. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project has provided training and professional development opportunities for two postdocs (Hossam Abdelhamed and Seongwon Nho), three doctoral students (Ali Akgul and Safak Kalindamar), and a visiting scholar (Iman Ibrahim). How have the results been disseminated to communities of interest?Results have been presented at scientific conferences and published in peer-reviewed journals. What do you plan to do during the next reporting period to accomplish the goals? Confirm safety and efficacy of the vaccine candidates in catfish fry. Repeat immersion-oral vaccination experiments in outside earthen ponds. Complete pathologic examinations. Determine adaptive immune responses.
Impacts
What was accomplished under these goals?
1) Major activities completed -Goal one: Determine virulence and efficacy of E. ictaluri mutants in catfish. Experiments measuring the safety of 35 mutants were conducted in catfish fingerlings. Experiments measuring the efficacy of 35 mutants were conducted in catfish fingerlings. Experiments measuring the safety of attenuated mutants were conducted in catfish fry. Experiments measuring the efficacy of attenuated mutants were conducted in catfish fry. Experiments measuring the optimal vaccination dose were conducted in catfish fry. Experiments measuring the optimal vaccination time were conducted in catfish fry. -Goal two: Determine the best vaccination regime using the most effective vaccine candidate. Experiments measuring the best oral vaccination dose were conducted. Experiments measuring the tissue persistence were conducted. Pathology experiments were conducted. 2) Specific objectives met -Goal one Safety and efficacy of all 35 mutants were determined in catfish fingerlings. Safety and efficacy of selected attenuated mutants were determined in catfish fry. Optimal immersion vaccination dose was determined. Optimal immersion vaccination time was determined. -Goal two Best oral vaccination dose was determined Tissue persistence of best vaccine candidates was determined. Pathologic effects of vaccine candidates were determined. 3) Significant Results Achieved We determined that two of the tested mutants have a good potential to be used as live attenuated vaccines in catfish fry. We determined that protective effect of vaccination is correlated with fry age. We determined that protective effect of vaccination increases significantly when fry are three weeks old or older. We determined that the best vaccination in fry under current production practices can be achieved by immersion vaccination and oral boosting after the fry are stocked into nursery ponds. We determined that vaccine candidates cause little pathologic changes in tissues. 4) Key outcomes or other accomplishments realized Two potential vaccine candidates for catfish fingerlings and fry were determined. Protection of vaccine candidates increases significantly when fry age is three weeks or older. Immersion vaccination and oral boosting by vaccinated feed provide the most effective protection in catfish fry.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Abdelhamed H, Lu J, Lawrence ML, Karsi A*. 2016. Ferric hydroxamate uptake system contributes to Edwardsiella ictaluri virulence. Microbial Pathogenesis. 2016 Sep 22;100:195-200. doi: 10.1016/j.micpath.2016.09.018. PMID: 27666508
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Kordon A, Scott M, Abdelhamed H, Ahmed Hamada, Ibrahim I, Baumgartner W, Karsi A, Pinchuk L. 2016. Identification of Langerhans-like cells in the immunocompetent tissues of channel catfish. Fish and Shellfish Immunology. 2016 Sep 16;58:253-258. doi: 10.1016/j.fsi.2016.09.033. PMID: 27645905.
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Abdelhamed H, Lu J, Lawrence ML, Karsi A*. 2016. Involvement of tolQ and tolR genes in Edwardsiella ictaluri virulence. Microbial Pathogenesis. 2016 Sep 10;100:90-94. doi: 10.1016/j.micpath.2016.09.011. PMID: 27622343.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2016
Citation:
May M, Kordon A, Abdelhamed H, Ahmed H, Karsi A, Pinchuk L. 2016. Antigen presenting cells promote protective immunity in catfish exposed to live Edwardsiella ictaluri vaccines. Merial-NIH Veterinary Scholars Symposium. Columbus, OH, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2016
Citation:
Kordon A, Abdelhamed H, Baumgartner W, Karsi A, Pinchuk L. 2016. Innate and adaptive immune responses of catfish antigen-presenting cells to live attenuated Edwardsiella ictaluri vaccines. 2nd International Conference of Fish and Shellfish Immunology. Portland, MA, USA.
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Progress 09/01/14 to 08/31/15
Outputs
Target Audience:Target Audiences Fish health research community Veterinarians Catfish producers Microbiologists Efforts Science-based knowledge has been delivered in laboratory teaching, fish health related courses and workshops. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project has provided training and professional development opportunities for two postdocs (Hossam Abdelhamed and Seongwon Nho), and two doctoral students (Ali Akgul and Safak Kalindamar). In addition, one visiting scholar (Iman Ibrahim) has been contributing to this project for the past six months. How have the results been disseminated to communities of interest?A catfish farm has been visited and ongoing research has been discussed with the owner. What do you plan to do during the next reporting period to accomplish the goals?Complete safety and efficacy testing of the remaining mutants in catfish fingerlings and conduct further testing of the safe and efficacious mutants in catfish fry. Conduct optimal immersion-oral vaccination experiments in outside earthen ponds. Complete pathologic examinations. Determine adaptive immune responses.
Impacts
What was accomplished under these goals?
1) Major activities completed -Goal one: Determine virulence and efficacy of E. ictaluri mutants in catfish. Experiments measuring the safety of 25 mutants were conducted in catfish fingerlings. Experiments measuring the efficacy of 25 mutants were conducted in catfish fingerlings. Experiments measuring the safety of two mutants were conducted in catfish fry. Experiments measuring the efficacy of two mutants were conducted in catfish fry. Experiments measuring the optimal vaccination time were conducted in catfish fry. -Goal two: Determine the best vaccination regime using the most effective vaccine candidate. Experiments measuring the best oral vaccination dose were conducted. Experiments measuring the tissue persistence were conducted. 2) Specific objectives met -Goal one Majority of mutants (25 out of 35 proposed) were screened for their safety and efficacy in catfish fingerlings and fry. Further screening of mutants in catfish fingerlings is underway, which will be extended to catfish fry when fry are available in early spring. Optimal immersion vaccination time were determined. -Goal two Best oral vaccination dose was determined Tissue persistence of best vaccine candidates were determined. 3) Significant Results Achieved We have determined that two of the tested mutants so far have a good potential to be used as live attenuated vaccines in catfish fry. We have determined that the best vaccination in fry under current production practices can be achieved by immersion vaccination and oral boosting after fry are stocked into nursey ponds. 4) Key outcomes or other accomplishments realized Two potential vaccine candidates for catfish fingerlings and fry were determined. An immersion-oral vaccination protocol is currently being evaluated.
Publications
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Abdelhamed H, Ibrahim I, Lu J, Lawrence ML, Karsi A. 2015. Evaluation of the role of TonB-dependent hemoglobin receptor in Edwardsiella ictaluri virulence. Food Security for the Future: The Role of Aquatic Health, Starkville, MS, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Kordon A, Scott M, Baumgartner W, Karsi A, Pinchuk L. 2015. Identification of dendritic cells in channel catfish. Food Security for the Future: The Role of Aquatic Health, Starkville, MS, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Ibrahim I, Abdelhamed H, Baumgartner W, Lawrence A, Ebegawy M, Lawrence ML, Karsi A. 2015. Histopathological and ultrastructural analysis of catfish fry tissues challenged with Edwardsiella ictaluri. Food Security for the Future: The Role of Aquatic Health, Starkville, MS, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Akgul A, Nho S, Lawrence ML, Karsi A. 2015. The role of universal stress proteins in Edwardsiella ictaluri virulence. American Society for Microbiology South Central Branch Meeting, Hattiesburg, MS, USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Kalindamar S, Abdelhamed H, Tekedar H, Lawrence ML, Karsi A. 2015. Transposon derived Edwardsiella ictaluri mutants and their vaccine potential. American Society for Microbiology 115th General Meeting, New Orleans, LA, USA.
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