Progress 10/01/16 to 09/30/17
Outputs
Target Audience:Professional researchers in the international seed and plant research community. Students at the national and international universities majoring plant and agricultural sciences. Global agricultural industry. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project provided opportunities for a graduate student to complete her PhD in 2017 and an industry researcher for his training in academia. How have the results been disseminated to communities of interest?The outcomes of the project were disseminated through presentations at conferences, such as the American Society of Plant Biologists 2017 and invited seminar at Sumika Agro Tech, Japan. What do you plan to do during the next reporting period to accomplish the goals?We plan to expand our collaboration with the Japanese and Australian group. For the PHS prevention project, we expect to obtain some results from transgenic wheat next year. For the DOGL4 project, we also expect the first outcomes from soybean. We plan to publish the DOGL4 results in Arabidopsis with the Dutch and Japanese collaborators in 2018.
Impacts
What was accomplished under these goals?
(1) Identifying key factors involved in the enhancement or loss of seed quality. Characterization of DELAY OF GERMINATION1-LIKE 4 (DOGL4), which was identified by the previous reporting period, was continued and expanded. DOGL4 has great potential to increase protein (and possibly lipid) contents in seeds. The increase of specific proteins by DOGL4 induction in Arabidopsis seeds have been confirmed by mass spec analysis. The information obtained from the model plant Arabidopsis has been translated into soybean, in collaboration with a research group in Hokkaido University, Japan. Transgenic plants are currently investigated. (2) Eliminating seed dormancy as a constraint during seed production and germination in agronomic seed production and ecological/biomass seed establishment. We had established seed germination suppression system through amplification of ABA biosynthesis in developing Arabidopsis seeds for technology development to prevent preharvest sprouting (PHS) in cereal crops. We demonstrated that even the hyperdormancy created by this system can be eliminated by 3′-hexylsulfanyl-ABA (AS6), an ABA antagonist, which induces temporal ABA insensitivity. These two approaches established foundation of PHS prevention technology. Now, the concept is being translated into wheat and barley, in collaboration with Japanese and Australian groups.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Sall K, Hendrix D, Sekine T, Katsuragawa Y, Koyari R, Nonogaki H. 2017. Transcriptomics of nine-cis-epoxycarotenoid dioxygenase 6 induction in imbibed seeds reveals feedback mechanisms and long noncoding RNAs. Seed Science Research, doi: 10.1017/S0960258517000216.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Nonogaki H. 2017. Seed biology updates - highlights and new discoveries in seed dormancy and germination research. Frontiers in Plant Science 8:524. doi: 10.3389/fpls.2017.00524.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Nonogaki M, Nonogaki H. 2017. Prevention of preharvest sprouting through hormone engineering and germination recovery by chemical biology. Frontiers in Plant Science 8:90. doi: 10.3389/fpls.2017.00090.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2017
Citation:
Sall K, Nonogaki M, Katsuragawa Y, Hendrix D, Dekkers JWD, Bentsink L, Nonogaki H. 2017. DELAY OF GERMINATION1-LIKE4 is a master regulator of reserve accumulation in seeds. Plant Biology 2017 (Honolulu, HI)
- Type:
Other
Status:
Published
Year Published:
2017
Citation:
Nonogaki M, Nonogaki H. 2017. Seed dormancy through enhanced ABA biosynthesis and germination recovery by ABA antagonists. Plant Biology 2017 (Honolulu, HI)
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2017
Citation:
Sall, K. 2017. The Analysis of Hormone-dependent Transcription Factors and LncRNAs in seeds by RNA Sequencing. Ph.D. Disertation. Oregon State University.
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Progress 10/01/15 to 09/30/16
Outputs
Target Audience:Professional researchers in the international seed and plant research community Students at the national and internaitonal universities majoring plant and agricultural sciences Global agricultural industry Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project provided opportunities for a graduate student and a scientist for career development. A PhD student was able to give an oral presentation in the international workshop in Canada and a scientist in the project was able to give an oral presentation in Australia, both of which were excellent professional and international experiences for them. The project also accommodated an industry trainee, which enhanced academia-industry interaction. How have the results been disseminated to communities of interest?The outcomes of the project were disseminated through presentations at international conferences, such as the 5th Workshop on the Molecular Aspects of Seed Dormancy and Germination and the 13th International Symposium on Preharvest Sprouting in Cereals, to which the PI was invited as the lead scientific committee and a keynote speaker. Invited seminars atCSIRO (Canberra, Australia), Hokkaido University (Sapporo, Japan) andSumika Agro Tech (Niihama, Japan), were also important dissemination activities to academia and industry. What do you plan to do during the next reporting period to accomplish the goals?The technology, which we have developed to prevent preharvest sprouting in cereals, is being transferred to wheat, barley and rice. In the next reporting period, we anticipate to recover crops carrying this technology, in collaboration with global research institute, including those in Australia, Canada, and Japan. Similar global collaborations are also undertaken for examination of the DOGL4 gene that has great potential to increase seed proteins and lipid in crops. These activities may not be complete only in the next reporting period. However, the outcomes will probably make more impacts on our society and perfectly address the goals of this project.
Impacts
What was accomplished under these goals?
(1) Identifying key factors involved in the enhancement or loss of seed quality. The genes identified by RNA sequencing (RNA-seq) of NCED6 (nine-cis-epoxycarotenoid dioxygenase 6: ABA biosynthesis gene)-inducible lines, which were described for the previous reporting period, were characterized into details. DELAY OF GERMINATION1 (DOG1)-LIKE 4 (DOGL4), which was identified as an outstanding ABA-regulated gene by RNA-seq, has been particularly focused on. DOGL4-inducible lines have been created using the Plant Gene Switch System (PGSS) and another round of RNA-seq was performed. The sequencing results revealed that DOGL4 is a master regulator of seed storage proteins and has great potential to enhance quality of seeds as the final products in agriculture and food. An intergenic long non-coding RNA (lincRNA), which was also upregulated by NCED6 induction (termed N6LINCR1), was characterized. For N6LINCR1, a manuscript has been submitted and is currently under review. (2) Eliminating seed dormancy as a constraint during seed production and germination in agronomic seed production and ecological/biomass seed establishment. This project had developed a technology, which imposes hyperdormancy through enhanced ABA production and prevents precocious seed germination, such as preharvest sprouting (PHS). As a next step, the germination recovery strategy through expression of ABA counteracting genes, such as GA biosynthesis gene or NCED RNAi was proposed (see previous report). Another germination recovery strategy, through a chemical biology approach, was tested in this reporting period. Application of 3′-hexylsulfanyl-ABA (AS6), an ABA antagonist, was sufficient to recover germination from our inducible hyperdormant seeds. While ABA biosynthesis was still enhanced in the hyperdormant seeds, AS6 caused temporal ABA insensitivity in seeds, which released deep dormancy. These results demonstrate that chemical biology approaches have great potential to eliminate constraints for seed production and germination in agriculture.Combination of hyperdormancy and germination recovery will establish a comprehensive technology for PHS prevention for cereals.
Publications
- Type:
Book Chapters
Status:
Awaiting Publication
Year Published:
2017
Citation:
Bewley, JD, Nonogaki H. Seed maturation and germination. Reference Module in Life Sciences, Elsevier, Oxford.
- Type:
Book Chapters
Status:
Published
Year Published:
2016
Citation:
Nonogaki M, Nonogaki H. 2017. Germination. In B. Thomas, B. G. Murray, D. J. Murphy eds, Encyclopedia of Applied Plant Sciences, Vol. 1, Waltman M. A.,Academic Press, pp. 509-512.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2016
Citation:
Nonogaki M. 2016. Strategies to recover germination from PHS-resistant hyperdormant seeds. 13th International Symposium on Preharvest Sprouting in Cereals (Perth, Australia)
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2016
Citation:
Nonogaki H. 2016. Prevention of preharvest sprouting through alteration of hormone metabolism, perception and signal transduction. 13th International Symposium on Preharvest Sprouting in Cereals (Perth, Australia)
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2016
Citation:
Nonogaki M. 2016. Prevention of preharvest sprouting through hormonal regulation. Preharvest Sprouting Workshop. (Kitami, Japan)
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2016
Citation:
Sall K, Nonogaki H. 2016. Biochemical function of DELAY OF GERMINATION1-LIKE4 in hormone and cell signaling. 5th Workshop on the Molecular Aspects of Seed Dormancy and Germination (Vancouver, Canada)
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2016
Citation:
Nonogaki M, Nonogaki H. 2016. Germinaiton recovery from the NCED-enhanced hyperdorrmant seeds using nitrate-inducible gene switch and chemical biology with an ABA antagonist. 5th Workshop on the Molecular Aspects of Seed Dormancy and Germinaiton (Vacouver, Canada)
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Progress 10/01/14 to 09/30/15
Outputs
Target Audience:Professional researchers in the international seed and plant research community Students at the national and internaitonal universities majoring plant and agricultural sciences Global agricultural industry Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Khady, Sall, a PhD student at Oregon State University has been trained under the objectives described above. She has learned basics of molecular genetics using Arabidopsis. We have also accommodated an industry trainee from an international seed company. The interaction between the PhD student and the industry trainee had synergistic effects in training. How have the results been disseminated to communities of interest?The outcomes of the project have been disseminated through journal publication and book chapters reported above. In addition, the PI was invited professor at Wageningen University, The Netherlands for three months, during which the project outcomes were also disseminated through seminars and lectures. The information was disseminated not only to professors and students at the university but also to industry researchers, for example, at the Dutch Seed Symposium 2015. In addition, PI was invited to the Brazilian Seed Congress where the project outcomes were disseminated to approximately 1,500 participants. The PI was also invited to research seminars/meetings in France, Germany and Japan, which also provided opportunities of dissemination of research outcomes. What do you plan to do during the next reporting period to accomplish the goals?We plan to expand our analysis on DOGL4 and ABAIR1, especially focusing on knockout plants and chemically inducible gene expression. RNA sequencing will also be expanded. The major objective is to identify genes up- and down-regulated by these factors to dissect molecular mechanisms associated with seed dormancy and germination. We also plan to apply the technologies, which we have developed, to agricultural crops, such as wheat. However, progress in this area will largely depend on collaborators in Canada. In any case, we will try to promoter "translational biology".
Impacts
What was accomplished under these goals?
(1) Identifying key factors involved in the enhancement or loss of seed quality. Abscisic acid (ABA) has negative effects on seed germination and causes slow stand establishment with low vigor. On the other hand, lack of ABA could cause wilty plants and seed maturation problems. It is necessary to control ABA-regulated events in seeds and plants for agricultural production. To this end, it is essential to understand the mechanisms of ABA-regulated biochemical and molecular events in seeds and plant. We took advantage of NCED (nine-cis-epoxycarotenoid dioxygenase; ABA biosynthesis enzyme) increase in seeds in one of our experimental systems, to identify more factors associated with ABA regulation in seeds. We performed RNA sequencing under normal vs. high ABA conditions. We identified DELAY OF GERMINAITON1-LIKE 4 (DOGL4) as one of the ABA up-regulated gene, which we are currently characterizing. In addition to many other coding genes, we also identified ABA-induced long non-coding RNAs, which we named ABAIRs. We are currently characterizing function of ABAIR1. (2) Eliminating seed dormancy as a constraint during seed production and germination in agronomic seed production and ecological/biomass seed establishment. We had developed dormancy-inducing technoloy by using a chemically inducible gene expression (Plant Gene Switch System: PGSS) in collaboration with Prof. Roger Beachy, Universiy of California, Davis. In addition, we develoed a new system of spontaneous dormancy, which does not require chemical induction. Both systems successfully induced seed dormancy, whcih can be appllied to preventing pecocious germinaiton, such as preharvest sprouting (PHS) in cereal crops. However, these approaches require a counteracting technology to recover germinaiton and rescue viable seeds. For this purpose, another chemically inducible gene expression system was developed using a nitrate-inducible promoter. Briefly, nitrate itself has dormancy releasing effects through upregulation of ABA deactivation genes. If RNA interefence against ABA biosynthesis genes are combined by this approach, dual effects of seed germination promotion is expected. This will be a perfect answer to the obejective "Eliminating seed dormancy as a constraint during seed production and germination".
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Nonogaki M, Sekine T, Nonogaki H. 2015. Chemically inducible gene expression in seeds before testa rupture. Seed Science Research 25, 345-352.
- Type:
Book Chapters
Status:
Awaiting Publication
Year Published:
2016
Citation:
Nonogaki M, NonogakiH. Seed development: Germination. In B. Thomas, B. Murray, D. Murphy eds, Encyclopedoa of Applied Plant Sciences, Second Edition Biology, Elsevier, Oxford, in press.
- Type:
Other
Status:
Other
Year Published:
2015
Citation:
Nonogaki H. 2015. Understanding the mechanisms of seed dormancy and germination - Advances, perspectives and new directions. XIX Brazilian Seed Congress (Foz do Iguazu, Brazil)
- Type:
Other
Status:
Other
Year Published:
2015
Citation:
Nonogaki H. 2015. Emerging mechanisms of seed dormancy and germination - the potential for future seed technologies and breeding. The 4th Dutch Seed Symposium by Plantum (Wageningen, The Netherlands)
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