Progress 09/01/14 to 08/31/15
Outputs
Target Audience:The professional development grant targeted to develop expertise in cutting-edge technology among 1890 faculties. Dr. Theodore W Thannhauser, a protein chemistry in USDA/ARS, has mentored the project PI (Dr. Suping Zhou at Tennessee State University) to conduct research projects, preparation of course works, training graduate students, and preparation of manuscripts and research proposals. During the grant period, the mentor and mentee have published one paper, mentored six TSU graduate students and reached out to international communities by offering demonstrations and lectures of plant proteomics. Additionally, we havesubmitted monthly projectprogress report to administrators to seek support in promoting the education and research in Plant Sciences. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Beyond the quantifiable outcomes represented by the technology transfer that took place as a direct result of this project are its more difficult to quantify successes. Among these the most important is that while resident at the Holley Center Dr. Zhou and her students became a part of a diverse collegial community of scientists that span three of the great research institutions of plant biology: the R.W. Holley Center for Agriculture and Health, the Boyce/Thompson Institute for Plant Science and Cornell University. The personal contacts made amount to a network of both technical knowledge and knowhow that will continue to grow through time providing benefits for years into the future. How have the results been disseminated to communities of interest?While conducting this project, we have provided demonstration/ and training to six TSU graduate students, one research associate, two international visiting scholar from China. Two graduate students were able to independently conduct the LCM procedure at the end of the training sessions. They will continue to use the technologyin competing their theses. In-house seminars were given to graduate students in the College of Agriculture at TSU. One special topic presentation was given to a 35 student class in the Department of Biology at TSU. Immediately upon completion of the experiments, contents and outcomes of the project were introduced to 42 graduate students, and three faculties through a graduate course 'Methodologies in Horticultural Sciences' which was offered in Nanjing Agricultural University, China in August 25-29. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
The primary goal of this project is to empower the 1890 faculties with emerging technology and thus enhance the teaching and research competitiveness of these institutions. The first outcome of the project is that the project PI (Dr. Suping Zhou at Tennessee State University) has become highly experienced in single-cell-type-proteomics, which will enable her to lead the Plant Proteomics Project in her home university. The second outcome is the development of a delivery mechanism to teach and train students in 1890 universities and beyond. Specifically, the Project has provided thefollowing opportunities for training and professional development: A. The training and technology development in laser capture microdissection (LCM) -proteomics The professional training activities were primarily conducted in Dr. Thannhauser's lab in the USDA/ARS, Robert W. Holley Center. The preparation and cryotome sectioning of plant samples were instructed by Miss Laetitia Martin in Dr. Jocelyn K.C. Rose's lab. The LCM and Confocal Microscope were instructed by Dr. Johanna M. Dela Cruz and Carol J. Bayles in the Imaging Facility at the Institute of Biotechnology, Cornell University. Under the mentorship of Dr. Thannhauser, Dr. Zhou and the project group (Yang Yong, Kevin J. Howe, Tara Fish, and TSU graduate students) have developed a work-flow for collection of samples from individual cell types in tomato roots, protein extraction from small amounts of sample, and 1D gel-LC-mass spectrometry proteomics analysis. We were able to identify a large number of proteins (up to 1700) in LCM-cell samples containing material isolated from as few as, 1,000-3,000 cells. Furthermore, we were able to leverage these project products to develop a new research proposal with the goal of understanding the impact of heat stress on pollen infertility and embryo abortion in tomato on a molecular level. This proposal was recently submitted to NIFA exploratory research program (USDA/NIFA/AFRI-004915; Grant11996844). B. Preparation of TSU graduate course 'Proteomics' The course will be offered in a hybrid style, with on-line lectures and traditional laboratory sections. Syllabi have been prepared where several lectures will be taught by Dr. Thannhauser. Videos have been shot demonstrating the following procedures: Isobaric labeling using iTRAQ reagents and two dimensional electrophoresis of proteins (assisted by Ms. Tara Fish and graduate student, Miss Hui Li); and LCM (provided by Dr. Johanna M. Dela Cruz). C. Functional genomics and bioinformatics Diane M Dunham has provided a demonstration on the virus-induced gene silencing (VIGS) procedure. This activity was coordinated by Dr. Sarah Refi Hind in Dr. Gregory Martin's group in BTI. The PI has learnt how to use the bioinformatics tools in the Sol for tomato proteomics and transcriptome data analysis, by working with Mr. Noe Fernandez in Dr. Mueller's group. In addition to the project activities described above , the following people have made this training program more productive: Dr. Li-Li has generously allocated her greenhouse space to accommodate the tomato plants which made it possible for us to collect root tissues on site. Dr. James J. Giovannoni from BTI kindly provided tomato flowers for our preliminary studies on pollen protein analysis. These results were used for preparation of a new research proposal. Mr. Eric C. Craft in Dr. Leon Kochian's group has installed GEOCHEM-EZ program and has taught me how to use the program to determine Al3+ activity in solution. This will help us with the ongoing Al-research project.
Publications
- Type:
Book Chapters
Status:
Published
Year Published:
2015
Citation:
Suping Zhou, Theodore W. Thannhauser. 2015. Chapter 6: Plant Cell and Organellar Proteomics and Salinity Tolerance in Plants. In Shabir Hussain Wani, Mohammad Anwar Hossain Eds. Managing Salt Tolerance in Plants: Molecular and Genomic Perspectives. CRC Press, ISBN 9781482245134 - CAT# K23522
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Progress 09/01/14 to 08/31/15
Outputs
Target Audience:The professional development grant targeted to develop expertise in cutting-edge technology among 1890 faculties. Dr. Theodore W Thannhauser, a protein chemistry in USDA/ARS, has mentored the project PI (Dr. Suping Zhou at Tennessee State University) to conduct research projects, preparation of course works, training graduate students, and preparation of manuscripts and research proposals. During the grant period, the mentor and mentee have published one paper, mentored six TSU graduate students and reached out to international communities by offering demonstrations and lectures of plant proteomics. Additionally, we have submitted monthly project progress report to administrators to seek support in promoting the education and research in Plant Sciences Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Beyond the quantifiable outcomes represented by the technology transfer that took place as a direct result of this project are its more difficult to quantify successes. Among these the most important is that while resident at the Holley Center Dr. Zhou and her students became a part of a diverse collegial community of scientists that span three of the great research institutions of plant biology: the R.W. Holley Center for Agriculture and Health, the Boyce/Thompson Institute for Plant Science and Cornell University. The personal contacts made amount to a network of both technical knowledge and knowhow that will continue to grow through time providing benefits for years into the future. How have the results been disseminated to communities of interest?While conducting this project, we have provided demonstration/ and training to six TSU graduate students, one research associate, two international visiting scholar from China. Two graduate students were able to independently conduct the LCM procedure at the end of the training sessions. They will continue to use the technology in competing their theses. In-house seminars were given to graduate students in the College of Agriculture at TSU. One special topic presentation was given to a 35 student class in the Department of Biology at TSU. Immediately upon completion of the experiments, contents and outcomes of the project were introduced to 42 graduate students, and three faculties through a graduate course 'Methodologies in Horticultural Sciences' which was offered in Nanjing Agricultural University, China in August 25-29. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
The primary goal of this project is to empower the 1890 faculties with emerging technology and thus enhance the teaching and research competitiveness of these institutions. The first outcome of the project is that the project PI (Dr. Suping Zhou at Tennessee State University) has become highly experienced in single-cell-type-proteomics, which will enable her to lead the Plant Proteomics Project in her home university. The second outcome is the development of a delivery mechanism to teach and train students in 1890 universities and beyond. Specifically, the Project has provided the following opportunities for training and professional development: A. The training and technology development in laser capture microdissection (LCM) -proteomics The professional training activities were primarily conducted in Dr. Thannhauser's lab in the USDA/ARS, Robert W. Holley Center. The preparation and cryotome sectioning of plant samples were instructed by Miss Laetitia Martin in Dr. Jocelyn K.C. Rose's lab. The LCM and Confocal Microscope were instructed by Dr. Johanna M. Dela Cruz and Carol J. Bayles in the Imaging Facility at the Institute of Biotechnology, Cornell University. Under the mentorship of Dr. Thannhauser, Dr. Zhou and the project group (Yang Yong, Kevin J. Howe, Tara Fish, and TSU graduate students) have developed a work-flow for collection of samples from individual cell types in tomato roots, protein extraction from small amounts of sample, and 1D gel-LC-mass spectrometry proteomics analysis. We were able to identify a large number of proteins (up to 1700) in LCM-cell samples containing material isolated from as few as, 1,000-3,000cells. Furthermore, we were able to leverage these project products to develop a new research proposal with the goal of understanding the impact of heat stress on pollen infertility and embryo abortion in tomato on a molecular level. This proposal was recently submitted to NIFA exploratory research program (USDA/NIFA/AFRI-004915; Grant11996844). B. Preparation of TSU graduate course 'Proteomics' The course will be offered in a hybrid style, with on-line lectures and traditional laboratory sections. Syllabi have been prepared where several lectures will be taught by Dr. Thannhauser. Videos have been shot demonstrating the following procedures: Isobaric labeling using iTRAQ reagents and two dimensional electrophoresis of proteins (assisted by Ms. Tara Fish and graduate student, Miss Hui Li); and LCM (provided by Dr. Johanna M. Dela Cruz). C. Functional genomics and bioinformatics Diane M Dunham has provided a demonstration on the virus-induced gene silencing (VIGS) procedure. This activity was coordinated by Dr. Sarah Refi Hind in Dr. Gregory Martin's group in BTI. The PI has learnt how to use the bioinformatics tools in the Sol for tomato proteomics and transcriptome data analysis, by working with Mr. Noe Fernandez in Dr. Mueller's group. In addition to the project activities described above , the following people have made this training program more productive: Dr. Li-Li has generously allocated her greenhouse space to accommodate the tomato plants which made it possible for us to collect root tissues on site. Dr. James J. Giovannoni from BTI kindly provided tomato flowers for our preliminary studies on pollen protein analysis. These results were used for preparation of a new research proposal. Mr. Eric C. Craft in Dr. Leon Kochian's group has installed GEOCHEM-EZ program and has taught me how to use the program to determine Al3+ activity in solution. This will help us with the ongoing Al-research project.
Publications
- Type:
Book Chapters
Status:
Published
Year Published:
2015
Citation:
Suping Zhou, Theodore W. Thannhauser. 2015. Chapter 6: Plant Cell and Organellar Proteomics and Salinity Tolerance in
Plants. In Shabir Hussain Wani, Mohammad Anwar Hossain Eds. Managing Salt Tolerance in Plants: Molecular and
Genomic Perspectives. CRC Press, ISBN 9781482245134 - CAT# K23522
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