Progress 10/01/14 to 09/30/19
Outputs
Target Audience:Our target audience is KAES researchers in the state of Kansas. This includes researchers on the KSU campus in Manhattan KS as well as a number of extension faculty located throughout the state. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?All members of the lab and all users of the equipment (faculty, research associates and students) are taught about and encouraged to learn the specific techniques and equipment. They are expected to participate as fully in the lab as they like. We have trained many students on peptide synthesis, various chromatography methods, and proteomics, along with the basic running of other machines in the core facility. The students have a good idea of how the lab and machines function, how to work with samples, and how to maintain the high standards of the core facility. How have the results been disseminated to communities of interest?Initially we meet with a new collaborator to discuss their goals for the project, their protocol, the necessary materials, and our limitations in the core facility. We encourage participation and involve them and their students or technicians in the data collection process as much as they would like to be involved. Once the data is collected, we meet or talk again and go over what was found and what it means for their specific work. There are times when these are brief and the meetings are simple, and there are other times that these meetings must be repeated while we continuously re-work the project and the methods. If the investigators would like to publish the data, we review the data, provide the necessary protocols for the manuscript, and review the final article to ensure it is appropriate. Finally, members of this lab and others have presented our data and contributions as regional and national meetings. What do you plan to do during the next reporting period to accomplish the goals?We plan to continue providing existing services we have in the past as well as add new services that relate to or upgrade our current instruments as to widen our abilities to work with new and current researchers. We have also recently upgraded our circular dichroism machine's software and temperature regulation apparatus. This allows more direct and precise control of the temperature which will benefit all users. We also are planning to get more liquid chromatography capabilities within the next year.
Impacts
What was accomplished under these goals?
One of major products is custom synthesized oligo nucleotides for campus laboratories. More than 35 AES supported investigators use this service. This past year we processed more than 1,200 orders, almost all of which have multiple products within the order, and many have five or more products. This has saved the investigators over $30,000 by negotiating a reduced cost for the product as well as eliminating all shipping costs. The following section describes the projects that completed or initiated in the lab this past year. The Core Facility is still working with Heartland Plant innovations to determine specific protein production and quantity in wheat strains produced. This protein will potentially be used to increase the flavor of breads with less added sugar. We have continued work with SmartVet Holdings of Olathe Kansas. We have determined composition of blended insecticides already approved for livestock. Work has also continued with them for solubilizing and blending services of the topical parasiticides/insecticides spinosad, diflubensuron and permethrin. We have been working with USDA-APHIS in Ft Collins CO on a collaborative process for evaluating the oral delivery of dsRNA to Feral Pigs We are also working with Greenlight Technology of Medford, MA on efficiently delivering dsRNA of plant leaves to control chewing insects. On campus we are currently assisting/consulting on protein isolation and identification, proteomics, epitope identification, protein structure identification and metabolomics with the laboratories of Philip Klebba, Brian Geisbrecht, Gerald Reeck, Timothy Durrett, George Wang, Sherry Fleming, Philip Hartwidge, Juergen Richt, Kathrin Schrick, Forrest Chumley, David Cook, Harold Trick, Ho Ng, Jayedra Amamcharla, Michal Zolkiewski, Brian Lindshield, and Stephanie Shames. In addition to those services, we have done custom peptide synthesis for these researchers on campus: myself John Tomich, Bruce Schultz, Jeremy Schmit, and Brian Geisbrecht. We are also working with Richard Todd to develop a new delivery mechanism for antifungal drugs or compounds. As well as showing the biodegradability of our peptide based nucleic acid delivery system. The Biotech Core Facility is also working with Michael Kanost in developing insecticide and DNA delivery systems to Manduca sexta that can prevent unwanted plant or environmental effects. Environmental- The Core Lab in collaboration with AES supported professors will offer proteomic and metabolomic analyses, as well as other services required, for numerous AES projects. Some of the specific organisms being studied are grain storage pests, insects detrimental to plants, or disease transmitting ones. These species include crickets, tobacco hornworm, red flour beetle, yellow mealworm, mosquitoes, ticks, aphids, thrips and other plant viruses or pathogens. We are working with projects aimed at developing DNA vaccines and delivery methods targeting animal virus infections. We are also working on citrus greening disease and other plant diseases. Our aggregate research efforts should eventually prevent dollar loses caused by crop failure, food spoilage or human/animal diseases. Economic: By having these facilities and capabilities here, we are able to assist KAES researchers and many others with their highly technical syntheses and analyses. This reduces the time and costs of their research and increases the productivity. Our availability as consultants to researchers at KSU is another big asset. The Biotech Core Facility provides methodology sections to grant applicants for inclusion in proposals. The proteomics and metabolomics studies included in plant and arthropod proposals has made them more competitive and helped a number of researches obtain extramural funding. Another benefit of having a fully functioning core lab is its value in recruiting new faculty. We routinely are included in the tours of potential faculty from the Colleges of Agriculture, Vet Med and Arts and Sciences. Many candidates have voiced their interest in working with the Core Facility and indicated that the lab stands as a testament to the commitment of the Deans for excellence in Research. One important benefit of having a full-service core lab at KSU is our ability to accept contract work from outside universities and companies. We are currently working with Heartland Plant innovations, SmartVet Inc., Phoreus Biotech, GreenLight Biosciences. In the past, researchers at major non Kansas universities- The State Universities of Alabama, Birmingham; Arizona; California- San Diego; California- Los Angeles; California- Merced; Colorado; Michigan; Montana; Virginia; Oregon; Washington: Missouri, KC; and Wisconsin; University of Pittsburgh, Scripps Institute; University of Southern California; Tufts University; Columbia University, NY; Washington University, St. Louis; Tulane University, Vanderbilt University, Baylor University Medical School, Harvard University and Auburn University, have used our services. Many of these schools have their own core labs but use ours based on our expertise. In recent years, we have worked for Nacelle Therapeutics, Amgen, Immunogen AB, Ligand Pharmaceuticals, Hill's Pet Foods, Sigma Diagnostics, Altecha Labs, Santa Cruz Biotechnology Inc., Trinity Biosciences, Ventria Inc., Allied Blending Inc., Aptakon, Inc., SmartVet Inc., Phoreus Biotech, and Nutri-Shield Inc. The university and corporate affiliations enhance our reputation and that of the research program at Kansas State University. Social: We will help society by decreasing dollar loses caused by food spoilage, insect, viral and other pathogen damage to agriculturally important crops, and human diseases by aiding veterinary, agricultural, arthropod, and biochemistry research
Publications
- Type:
Book Chapters
Status:
Accepted
Year Published:
2019
Citation:
Tomich, J. M. Wessel, E., Choi, J., Avila, L. A. (2019) Peptiplexes-A Review of Therapeutic Applications. In Nucleic Acid Nanotheranostics: Biomedical Applications Marco Filice and Jesus Ruiz-Cabello eds, Elsevier Publishing (Amsterdam, Netherlands) pp 247-276.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2019
Citation:
Delimont, N. M., Katz. B. B., Fiorentino, N. M., Kimmel, K. A., Haub, M. D., Rosenkranz, S. K., Tomich, J. M., Lindshield,
B. L. (2019) Salivary Cystatin SN Binds to Phytic Acid In Vitro and Is a Predictor of Nonheme Iron Bioavailability with Phytic Acid Supplementation in a Proof of Concept Pilot Study. Curr Dev Nutr. 3(7)
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Progress 10/01/17 to 09/30/18
Outputs
Target Audience:There have been over 100 different laboratories that this facility has assisted in the past decade. These laboratories come from all over the university and frommultiple laboratories within the Colleges of Arts and Sciences, Agriculture, Veterinary Medicine, and Human Ecology. The specific departments includetheDepartments of Biochemistry, Biology, Entomology, Anatomy and Physiology, Diagnostic Medicine and Pathobiology, PlantPathology, Agronomy, Horticulture, Grain Science, Human Nutrition and Animal Science Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?We train and encourage all members of the lab and all users of the equipment (faculty, research associates and students) to learn the techniques and equipment and to participate fully in the lab. We have trained many students on peptide synthesis, various chromatography methods, and proteomics, along with the basic running of other machines wiching the core facility. This not only allows those students to have a greater idea of how the lab and machines function, but they can better understand how to work with samples under the high standards of the core facility. How have the results been disseminated to communities of interest?When we begin a project with someone, initially we meet with them to discuss their goals for the project, their protocol, the necessary materials, and our limitations in the core facility. We involve them in the data collection process as much as they would like to be involved, and we encourage them to participate. Once the data is collected, we meet again and go over what was found and what it means for their specific work. There are times when these are brief and the meetings are simple. Other times, these meetings must be repeated over and over while we continuously re-work the project and the methods. If the investigators would like to publish the data, we review the data, provide the necessary protocols for the manuscript, and review the final article to ensure it is appropriate. Finally, members of this lab and others have presented our data and contributions as regional and national meetings. What do you plan to do during the next reporting period to accomplish the goals?The plan for this next reporting period is to increase the functionality of many of the machines within the lab. We would like to expand our liquid chromatography capabilities by getting another machine up and running. We have also begun an intenstive inventory and clean-out of the lab space, and this will also lead to increased productivity .
Impacts
What was accomplished under these goals?
A major service that we provide to multiple laboratories on campus is the custom synthesized oligonucleotides. During the past year, we have processed over 1,300 orders. These orders typically have multiple products in each order, and many of them even have 5 or more per order.By negotiating a reduced cost, those investigators, including over 50 AES supported investigators, have saved nearly $40,000 last year along with eliminating shipping costs from their orders. The following section describes projects that we have completed or initiated within the lab in the past year. We have continued to help Randall Tosh of SmartVet Holdings in Olathe, KS, identify adjuvants and carriers of mutliple pesticide formulations. In order to do this, we must reverse-engineer and identify small molecules and solvents within the solution. Once these have been identified, new blends can be made by using the appropriate FDA-approved solvents and mixtures for cattle. We also continue to work with the USDA NIFA projectcalled Developing an Infrastructure and Product Test Pipeline to Deliver Novel Therapies for Citrus Greening Disease. We have also continued work with a peptide-based transfection agent, and we are consulting with other laboratories within KSU and at other universities on this. Since last year, we have repaired the MALDI-TOF Mass Spectrometer so it is now fully functional. We also purchasedupdated software and temperature control system for our Circular Dichroism spectrometer to obtain more acurate and simple temperaure changes. Our schedule of charges now charges for the Circular Dichroism and HPLC use. On campus we are currently assisting/consulting on protein isolation, proteomics, epitope identification and metabolomics with the laboratories ofRichard Todd,Yonghui Li,Philip Klebba, Brian Geisbrecht, Erika Geisbrecht, Gerald Reeck, Michael Kanost, Timothy Durrett, JohnGonzalez, George Wang, Roman Ganta, Kristin Michel, Sherry Fleming, Sunghun Park, Yoonseong Park, Ying Fang,Kathrin Schrick, Meena Kumari, Forrest Chumley, Michael Choa, and David Cook.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2018
Citation:
Avila, L.A., Chandrasekar, R., Wilkinson, K.E., Balthazor, J., Heerman, M., Bechard, J., Brown, S., Park, Y., Dhar, S., Reeck, G.R., Tomich, J.M. (2018). Delivery of lethal dsRNA in insect diets by branched amphiphilic peptide capsules. Journal of Controlled Release. 273. 139-146.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2018
Citation:
Natarajan, P., Sukthankar, P., Changstrom, J., Holland, C.S., Barry, S., Hunter, W.B., Sorensen, C.M., Tomich, J.M. (2018). Synthesis and characterization of multifunctional branched amphiphilic peptide bilayer conjugated gold nanoparticles. ACS Omega. 3(9). 11071-11083.
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Progress 10/01/16 to 09/30/17
Outputs
Target Audience:Over the past ten years the facility has assisted more than 100 different laboratories supported by KAES. These laboratories are located in the Colleges of Agriculture, Veterinary Medicine, Human Ecology and Arts and Sciences within the Departments of Biochemistry, Biology, Entomology, Anatomy and Physiology, Diagnostic Medicine and Pathobiology, Plant Pathology, Agronomy, Horticulture, Grain Science, Human Nutrition and Animal Science. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?As a core lab, we encourage all users (faculty, research associates and students) to participate in and learn techniques that we provide. In the past year, we have trained mostly research associates and students in in both proteomic sample preparation and analysis as well as peptide synthesis. By including the other researchers in the preparation and analysis process, they can better understand how to make and analyze samples with the high standards of the core facility. How have the results been disseminated to communities of interest?This is accomplished through several mechanisms. For those who are new to working with us, we sit down with them in a meeting to go over the protocol, the needed materials, and what to expect. Once the data is collected, we assist with analysis of the data, and this data is often incorporated into scientific manuscripts or grant applications. We then review this data that was generated within the core facility and has been prepared by other investigators into a manuscript. The papers are sent out for review and ultimately published in scientific journals. Noteworthy publications are often accompanied by University generated press releases. Lastly our contributions are presented at regional and national meetings by many different labs and departments. What do you plan to do during the next reporting period to accomplish the goals?We plan to continue providing existing services we have in the past as well as add new services that relate to our instrumentation. Our mass spectrometer has been non-functioning for a few months, and funds have been secured to get this operational again. We have also recently ordered new parts for our circular dichroism machine to update the software and hardware to benefit all users.
Impacts
What was accomplished under these goals?
One of major products is custom synthesized oligo nucleotides for campus laboratories. More than 50 AES supported investigators use this service. This past year we processed more than 1000 orders, almost all of which have multiple products within the order, and many have five or more products. This has saved the investigators over $30,000 by negotiating a reduced cost for the product as well as eliminating all shipping costs. The following section describes the projects that completed or initiated in the lab this past year. In continuing projects with Randall Tosh of SmartVet Holdings of Olathe Kansas we have have helped them formulate a non-aqueous delivery vehicle for the delivery of the insecticides Abamectin and Eprinomectin for use in their paintball delivery system for cattle. The issues that we resolved involved finding the appropriate mix of FDA approved organic solvents that improved dermal uptake while keeping the active ingredient fully soluble for at least six months. We are still working with them on developing a DNA vaccine that can be delivered using their paintball gun technology. The Biotech Core lab has also become involved with a USDA NIFA project called Developing an Infrastructure and Product Test Pipeline to Deliver Novel Therapies for Citrus Greening Disease. We have continued working on a protein and peptide characterization project with Rich Suderman the Director of R&D for Nectagen Inc. in Kansas City, Kansas. On campus we are currently assisting/consulting on protein isolation, proteomics, epitope identification and metabolomics with the laboratories of-- Philip Klebba, Brian Geisbrecht, Erika Geisbrecht, Gerald Reeck, Michael Kanost, Timothy Durrett, John Gonzales, George Wang, Ping Li, Roman Ganta, Kristin Michel, Katsura Asano, Praveen Vadlani, Sherry Fleming, Brad Olsen, Sunghun Park, Yoonseong Park, Philip Hartwidge, Ying Fang, and Juergen Richt, Lisa Wilken, Kathrin Schrick, W. Zhang, Lorena Passarelli, Meena Kumari, Forrest Chumley. This past year, we have been doing less proteomics work due to technical issues with the mass spectrometer machine that was paramount in this research. These issues should be resolved shortly. Part of getting these machines fixed required securing of funds, which we have managed to do and we have now initiated a service call to the manufacturer (Bruker Instruments. We have also updated our schudule of charges and have begun charging for HPLC purifications and circular dichroism spectral analyses.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Obasa, K., Fellers, J. Kennelly, M., Katz, B., Tomich, J., Liu, S., Moore, D., Shinogle, H., Kelley, K., White, F. F. (2017) A dimorphic and virulence-enhancing endosymbiont bacteria discovered in Rhizoctonia solani. Phytobiome. 1(1) 14-23.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Balhesteros, H., Shipelskiy, Y., Long, N. J., Majumdar, A., Katz, B. B., Santos, N. M., Leaden, L., Newton, S. M., Marques, M. V., & Klebba, P. E. (2017) TonB-Dependent Heme/Hemoglobin Utilization by Caulobacter crescentus HutA. J Bacteriol.199(6). PMID: 28031282
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Woehl, J. L., Ramyar, K. X., Katz, B. B., Walker, J. K., & Geisbrecht, B. V. (2017) The structural basis for inhibition of the classical and lectin complement pathways by S. aureus extracellular adherence protein. Protein Sci. 26(8): 1595-1608. PMID: 28512867
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Teixeira, A. D. D., Games, P. D., Katz, B. B., Tomich, J. M., Zanuncio, J. C., & Serr�o, J. E. (2017) Proteomic analysis in the Dufour's gland of Africanized Apis mellifera workers (Hymenoptera: Apidae). PLoS One 12(5). PMID: 28542566
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Barros, S. M., Avila, L. A., Whitaker, S. K., Wilkinson, K. E., Sukthankar, P., Beltr�o, E. I. C.,& Tomich, J. M. (2017) Branched Amphipathic Peptide Capsules: Different Ratios of the Two Constituent Peptides Direct Distinct Bilayer Structures, Sizes, and DNA Transfection Efficiency. Langmuir 33(28): 7096-7104. PMID: 28654272
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Progress 10/01/15 to 09/30/16
Outputs
Target Audience:Over the past ten years the facility has assisted more than 100 different laboratories supported by KAES. These laboratories are located in the Colleges of Agriculture, Veterinary Medicine, Human Ecology and Arts and Sciences within the Departments of Biochemistry, Biology, Entomology, Anatomy and Physiology, Diagnostic Medicine and Pathobiology, Plant Pathology, Agronomy, Horticulture, Grain Science, Human Nutrition and Animal Science. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?As a core lab, we encourage all users (faculty, research associates and students) to participate in techniques that we provide. In the past year, we have trained mostly research associates and students in in both proteomic sample preparation and analysis as well as peptide synthesis. By participating in our studies the trainees better understand the key steps in producing high quality samples as well as learn the limitation of the technology. How have the results been disseminated to communities of interest?This is accomplished through several mechanisms. For newcomers, we sit down and explain our protocols and what to expect in one on one meetings. After data is collected and incorporated into scientific manuscripts or grant applications we review those components that were generated in the facility. The papers are sent out for review and ultimately published in scientific journals. Noteworthy publications are often accompanied by University generated press releases. Lastly our contributions are presented at regional and national meetings. What do you plan to do during the next reporting period to accomplish the goals?We plan to continue providing existing services as we have in the past, as well as add new services that relate to our instrument. In the past year we have replace two aging HPLC units and the coming year we will be replacing our freeze dryer unit and the rough pump on our MALDI TOF/TOF instrument.
Impacts
What was accomplished under these goals?
One of our major products is custom synthesized oligo nucleotides for campus laboratories. More than 50 AES supported investigators use this service. This past year we processed more than 1350 orders and saved the investigators over $33,000 by negotiating a reduced cost for the product as well as eliminating all shipping costs. The following section describes the projects that completed or initiated in the lab this past year. This past year we updated our schedule of charges. In continuing projects with Randall Tosh of SmartVet Holdings of Olathe Kansas we are helping them formulate a non-aqueous delivery vehicle for the delivery of the insecticide abamectin for use in their paintball delivery system for cattle. The issues that we resolved involved finding the appropriate mix of FDA approved organic solvents that improved dermal uptake while keeping the active ingredient fully soluble for at least six months. Numerous iterations have been tried thus far. The final blend has now been selected as of the time this report was prepared. The company recently completed a residue depletion study for this product and have filed the necessary documents with the EPA and are awaiting approval. We are still working with them on developing a DNA vaccine that can be delivered using their paintball gun technology. We submitted a USDA/NICHD grant entitled: Peptide Nano-Capsule Facilitated Delivery of Nucleic Acid Therapeutics to Livestock. The Biotech Core lab has also become involved with a USDA NIFA project called Developing an Infrastructure and Product Test Pipeline to Deliver Novel Therapies for Citrus Greening Disease. We continue working on a protein and peptide characterization project with Rich Suderman the Director of R&D for Nectagen Inc. in Kansas City, Kansas. Another service we provided this past year involved our analytical services in which we helped certify the safety of the milk supply in the State of Missouri. There was a possibility that a rodenticide may have gotten into the milk supply and all shipments were halted for several days. We were called in to assay the samples and show that the supply was not contaminated. We were able to provide USDA accepted results in less than 24 hours which allowed the milk supply to be released for sale. On campus we are currently assisting/consulting on protein isolation, proteomics, epitope identification and metabolomics with the laboratories of-- Philip Klebba, Brian Geisbrecht, Michael Kanost, John Gonzales, George Wang, Ping Li, Roman Ganta, Kristin Michel, Katsura Asano, Praveen Vadlani, Gerald Reeck, Sherry Fleming, Brad Olsen, Sunghun Park, Yoonseong Park, Philip Hardwidge, Ying Fang, and Juergen Richt, Lisa Wilken, Kathrin Schrick, W. Zhang, Lorena Passarelli, Meena Kumari, Forrest Chumley.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Games, P.D., Alves, S.N., Katz, B.B., Tomich, J.M., Serr�oa J. E. (2016) Differential proteins expression in the midgut of Culex quinquefasciatus induced by Temephos insecticide. Med Vet Entomol. 30(3):253-263. PMID: 27072633.
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Guo, R., Katz, B.B. Tomich, J.M., Gallagher, T., Fang, Y. (2016) Porcine reproductive and respiratory syndrome virus utilizes nanotubes for intercellular spread. J Virol. 90(10):5163-5175. PMID: 26984724.
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Barros, S. M., Whitaker, S. K., Sukthankar, P. R., Gudlur, S., Warner, M. Beltr�o, E. I. C., Tomich, J.M. (2016) A Review of Solute Encapsulating Nanoparticles used as Delivery Systems with Emphasis on Branched Amphipathic Peptide Capsules. Arch Biochem Biophys. 596: 22-42. PMID: 26926258.
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Jia, Z., Whitaker, S.K., Tomich, J. M., Chen, J. (2016) Organization and Structure of Branched Amphipathic Oligopeptide Bilayers, Langmuir 32: 98839891. PMID: 27584835.
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Avila, L.A., Aps, L. R. M. M., Sukthankar, P., Ploscariu, N., Games, P. D., Whitaker, S. K., Fang, Y., Szoszkiewicz, R., Ferreira, L. C. S., Tomich, J. M. (2016) Branched amphiphilic cationic oligo-peptides for delivery of plasmid DNA vaccines. J. Contrl Release. 241:15-24. PMID: 27592740.
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Obasa, K., Fellers, J. Kennelly, M., Katz, B., Tomich, J., Liu, S., Moore, D., Shinogle, H., Kelley, K., White, F. F. 2016. A dimorphic and virulence-enhancing endosymbiont bacteria discovered in Rhizoctonia solani.: Phytobiome. Manuscript ID: PBIOMES-08-16-0005-R.
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Progress 10/01/14 to 09/30/15
Outputs
Target Audience:We serve the research needs of many KAES University faculty, research fellows and students as well as regional Agribusinesses. This includes faculty members in Animal Science, Biochemistry, Biology, Plant Pathology, Veterinary Medicine, Horticulture, Nutrition and Grain Science. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?As a core lab we encourage all users (faculty, research associates and students) to participate in techniques that we provide. In the past year we have trained mostly research associates and students in both proteomic sample preparation and analysis as well as peptide synthesis. By participating in our studies the trainees better understand the key steps in producing high quality samples as well as learn the limitation of the technology. How have the results been disseminated to communities of interest?This is accomplished through several mechanisms. For new comers we sit down and explain our protocols and what to expect in one on one meetings. After data is collected and incorporated into scientific manuscripts or grant applications we review those components that were generated in the facility. The papers are sent out for review and ultimately published in scientific journals. Noteworthy publications are often accompanied by University generated press releases. Lastly our contributions are presented at regional and national meetings. What do you plan to do during the next reporting period to accomplish the goals?We plan to continue providing existing services we have in the past as well as add new services that relate to our instrument. This year we are in discussions with upper administration on how to lease a new mass spectrometer that will improve our sensitivity by 100-fold.
Impacts
What was accomplished under these goals?
One of our major products is custom synthesized oligo nucleotides for campus laboratories. More than 50 AES supported investigators use this service. This past year we processed more than 1200 orders and saved the investigators over $30,000 by negotiating a reduced cost for the product as well as eliminating all shipping costs. The following section describes the projects that completed or initiated in the lab this past year. In projects with Randall Tosh of SmartVet Holdings of Olathe Kansas we have been helping them formulate a non-aqueous delivery vehicle for the delivery of the insecticide abamectin for use in their paintball delivery system for cattle. The issues that we resolved involved finding the appropriate mix of FDA approved organic solvents that improved dermal uptake while keeping the active ingredient fully soluble for at least six months. Numerous iterations have been tried thus far. The final blend has not been selected as of the time this report was prepared. We are also working with them on developing a DNA vaccine that can be delivered using their paintball gun technology. They have support from the U.S. Department of Homeland Security to develop a traceable Hoof and Mouth Disease vaccine. The new protease treatment protocol we developed last year for wheat flour that generates short peptides from relatively indigestible gluten proteins is finally being tested at MGP ingredients of Atchison KS. If this protocol can be scaled up it could provide an antigen (gliadin) free product from wheat that should not trigger celiac disease in humans. We serve as consultants on this project. In a joint project with Midwest Veterinary Services of Oakland NE and Rheintek of Santiago, CHILE we prepared DNA vaccine carriers for oral administration in farm raised salmon. This project is still underway. We have also been working on a protein and peptide characterization project with Rich Suderman the Director of R&D for Nectagen Inc. in Kansas City, Kansas. On campus we are currently assisting/consulting on protein isolation, proteomics, epitope identification and metabolomics with the laboratories of-- Philip Klebba, Brian Geisbrecht, Michael Kanost, John Gonzales, George Wang, Ping Li, Phillip Klebba, Roman Ganta, Kristin Michel, Katsura Asano, Praveen Vadlani, Gerald Reeck, Sherry Fleming, Brad Olsen, Sunghun Park, Yoonseong Park, Philip Hartwidge, Ying Fang, Marcelo Ramalho-Ortigao and Juergen Richt.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Xu, J,. Su, X., Lim, S., Griffin, J., Carey, E., Katz, B., Tomich, J.M., Smith, J.S., Wang, W. (2015) Characterization and Stability of Anthocyanins in Purple-fleshed Sweet Potato P40. Food Chemistry 186: 90-96. PMID: 25976796
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Cui, F., Wang,W., Zhang, Y., Dai, H., Raman, C., Hiromasa, Y., Peng,G., Chen, X., Tomich, J., Reese, J., Edwards, O., Reeck, G. (2015) The protein Armet/MANF is responsive to endoplasmic reticulum stress in insects. FASEB J 5:2032-2045. PMID: 25678626
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Avila, L.A., Aps, L. R. M. M., Sukthankar, P., Ploscariu, N., Gudlur, G., `imo,L. Szoszkiewicz, R., Park, Y., Lee, S. W., Iwamoto, T., Ferreira, L., Tomich, J. M. (2015) Alternate supramolecular structures for different ratios of branched amphiphilic cationic peptide /DNA assemblies: Correlation with gene delivery Mol Pharm. 12(3):706-715. PMID: 25647162
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Chen, C., Cao, R., Shrestha, R., Ward, C., Katz, B., Fischer, C., Tomich, J.M., Li, P. (2015) Trapping of Intermediates with Substrate Analog HBOCoA in the Polymerizations Catalyzed by Class III Polyhydroxybutyrate (PHB) Synthase from Allochromatium Vinosum ACS Chem Biol. 10(5):1330-1339. PMID: 25686368.
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Sukthankar, P, Whitaker, S.K., Garcia, M., Katz, B.B., Tomich, J.M. (2015) Thermally Induced Conformational Transitions in Branched Amphiphilic Peptide Capsules. Langmuir 31(10):2946-2955. PMID: 25719598
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