Progress 09/01/14 to 08/31/17
Outputs
Target Audience:Over the course of the project, the targets were undergraduate students, mentors and support staff (research technicians and graduate students) in the disciplines of agriculture, environmental and life sciences at North Carolina A&T State University. The goal was to build a core group of faculty/staff with practical skills in basic proteomics approaches, while providing opportunities for students to learn and apply proteomics to their research projects. Faculty and support staff (i.e., research technicians) participated in hands- on proteomics training in the proteomics lab in the Department of Animal Sciences. Workshops were conducted by PD Waterman with the assistance of graduate students. Undergraduate students participated in experiential learning activities with faculty mentors to apply proteomics skills learned in the proteomics lab. PD Waterman also served as a guest lecturer/instructor for courses in the Departments of Animal Sciences, Biology and Natural Resources and Environmental Design. The target audience for courses included undergraduate and graduate (MS & PhD) students. Graduate students also participated in training activities which have made them more competitive for the job market. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?During the initial year of this three year project, two members of the proteomics lab (PD Waterman and doctoral student, C.J. McClendon) participated in a hands-on professional development/training activity at the UNC CH School of Pharmacy Proteomics Facility at to learn kinase proteome (kinome) analysis techniques (efforts were partially supported through a companion project funded through the N.C. TraCS Institute at UNC Chapel Hill). Existing gel based proteomics skills were enhanced by learning how new protein isolation and purification techniques to prepare samples for quantitative proteomics - label-based and label-free mass spectrometry analyses. During Year 2, two faculty members, one staff member, four graduate students (2 PhD and 2 MS) and five undergraduate students, and one high school intern participated in professional development and/or experiential learning and training opportunities. PD Waterman and graduate students from the proteomics lab trained undergraduates in laboratory basics and hands-on protein isolation and analysis skills, including basic micropipetting skills, protein isolation from cells and tissues, one-dimensional protein gel electrophoresis, imaging and image analysis. In the final year (year 3) of the project, the proteomics lab sponsored a two-day Western Blotting Workshop (October 26-27, 2016): PD Waterman, staff and graduate students from the proteomics lab trained nine undergraduate students (4), graduate students (2) and staff scientists (3) in laboratory basics and hands-on protein isolation and analysis skills, including basic micropipetting skills, protein isolation from cells and tissues, protein quantitation, gel electrophoresis, western blotting, protein imaging and analysis. How have the results been disseminated to communities of interest?Conferences and seminars. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
During Year 1 of this project, one course (ANSC 771) was enhanced to include proteomics instructional material which was developed and delivered by PD Waterman. During Year 2, several members of the proteomics lab received updated training in preparation for training others in Year 2. Specifically, PD Waterman and doctoral student (C.J. McClendon) learned and incorporated new protein extract clarification methods for low-abundance protein signal improvement into the standard procedures for the proteomics lab at N.C.A&T. Learning new technique is critical to stay up-to-date with the field of proteomics. Co-PD Worku continues to include proteomics course materials in the ANSC 771 course curriculum. In Year 2, three courses incorporated proteomics instructional material (28 students impacted through courses). During Year 3 seven undergraduate students were recruited to participate in proteomics-based experiential learning opportunities under the direction of a tenured faculty mentor (Drs. Waterman, Minor, Ongeri and Yang). One graduate student was mentored by Dr. Dukka KC in the area of bioinformatics. Three staff scientists and two graduate students participated in a proteomics-based professional development. Four courses (ANSC 637; ANSC 771 (taught by Co-PD Worku), LASC 636, LASC 660) continued to incorporate proteomics instructional material and PD Waterman served as a guest lecturer for HORT 600 for the first time; approximately 50 students were impacted through courses. Overall, as a result of this project, there is a core group of faculty actively incorporating proteomics into their teaching programs. Several of these faculty members are including proteomics approaches in their research programs, although in a limited capacity. The project has been successful in accomplishing the intended goals.
Publications
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Progress 09/01/16 to 08/31/17
Outputs
Target Audience:The target audience in year 3 was mainly undergraduate students and support staff in the disciplines of agriculture, environmental and life sciences at North Carolina A&T State University. Undergraduate students participated in experiential learning activities with faculty mentors to apply proteomics skills learned in the proteomics lab. PD Waterman served as a guest lecturer/instructor for courses in the departments of Animal Sciences and Natural Resources and Environmental Design. The target audience for courses included undergraduate and graduate (MS) students. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The proteomics lab sponsored a two-day Western Blotting Workshop (October 26-27, 2016): PD Waterman, staff and graduate students from the proteomics lab trained nine undergraduate students (4), graduate students (2) and staff scientists (3) in laboratory basics and hands-on protein isolation and analysis skills, including basic micropipetting skills, protein isolation from cells and tissues, protein quantitation, gel electrophoresis, western blotting, protein imaging and analysis. How have the results been disseminated to communities of interest?Results have been shared through conference presentations. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
During Year 3 seven undergraduate students were recruited to participate in proteomics-based experiential learning opportunities under the direction of a tenured faculty mentor. Three staff scientists and two graduate students participated in a proteomics-based professional development. Four courses (ANSC 637; ANSC 771, LASC 636, LASC 660) continued to incorporate proteomics instructional material and PD Waterman served as a guest lecturer for HORT 600 for the first time; approximately 50 students were impacted through courses. Enhanced access to proteomics expertise and technology at N.C. A&T was provided to address the national need for STEAM field professionals with proteomics expertise to make sense of the ever increasing number of genome sequences.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2017
Citation:
Waterman JT, McClendon CJ, Ranabhat R, Gilbert TS, Herring L, Graves LM. Swine Production Environment Modulates Kinome Signaling In Porcine Tracheobronchial Epithelial Tissues. American Journal of Respiratory and Critical Care Medicine 2017;195:A3894
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Horton CL, Ranabhat RS, Minor RC, Waterman JT. Intensive Swine Production Leads to Modulation Of Immunoglobulin A (IgA) In The Lower Airways Of Pigs. ARD Research Symposium 2017, Atlanta, GA, April 1-4, 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Locke KP, Harper M, Johnson ML, Ranabhat RS, McIntosh P, Conklin DR, Waterman JT. Continuous Exposure To Swine Production Facilities Leads To Inflammation And Oxidative Stress Signatures In Lungs And Blood Of Indoor Pigs. ARD Research Symposium 2017, Atlanta, GA, April 1-4, 2017.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2017
Citation:
Locke KP, Harper M, Johnson ML, Ranabhat RS, McIntosh P, Conklin DR, Waterman JT. Continuous Exposure to Swine Production Facilities Leads to Inflammation and Oxidative Stress Signatures in Lungs and Blood: A Porcine Model. American Journal of Respiratory and Critical Care Medicine 2017;195:A3887
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2017
Citation:
Ranabhat R, McClendon JC, Waterman JT. Housing Type Modulates Oxidant Levels and Antioxidant Defenses in Swine Airway Epithelial Tissue. American Journal of Respiratory and Critical Care Medicine 2017;195:A3888
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Harper M, Locke KP, Johnson ML, Ranabhat RS, Conklin DR, Waterman JT. Protein Content And Leukocyte Diversity As A Measure Of Porcine Lung Health. ARD Research Symposium 2017, Atlanta, GA, April 1-4, 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Ranabhat RS, McClendon CJ, Waterman JT. Housing Type Modulates Oxidant Levels And Antioxidant Defenses In Swine Airway Epithelial Tissue. ARD Research Symposium 2017, Atlanta, GA, April 1-4, 2017.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2017
Citation:
Ellison A, Barton KT, Ranabhat RS, Locke KP, Conklin DR, Minor RC, Waterman JT. The Levels Of Immunoglobulin E And Granulocytes In The Serum and Lung Lavage Of Indoor and Outdoor Reared Swine. N.C. A&T College of Agriculture and Environmental Sciences (CAES) Showcase of Excellence. Greensboro, NC. September 6, 2017.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2017
Citation:
Holmes-Cobb L, Barton KT, Ranabhat RS, Locke KP, Conklin DR, Minor RC, Waterman JT. Comparison Of Immunoglobulin M And Degranulated Neutrophil Levels In The Serum And Lung Lavage Of Indoor And Outdoor Reared Pigs. N.C. A&T College of Agriculture and Environmental Sciences (CAES) Showcase of Excellence. Greensboro, NC. September 6, 2017.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2017
Citation:
Lee N, Barton KT, Ranabhat RS, Locke KP, Conklin DR, Minor RC, Waterman JT. Effect Of Swine Production Buildings On Vacuolated Phagocytes And Immunoglobulin A Levels In Blood And Lung Lavage Of Pigs. N.C. A&T College of Agriculture and Environmental Sciences (CAES) Showcase of Excellence. Greensboro, NC. September 6, 2017.
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Progress 09/01/15 to 08/31/16
Outputs
Target Audience:The target audience in Year 2 included undergraduate recruited and trained by faculty mentors and graduate students in the disciplines of agriculture, environmental and life sciences at North Carolina A&T State University. Additionally, undergraduate and graduate in relevant courses (e.g., taught in the college of agriculture and life sciences, college of science and technology) that participated in proteomics workflows lectures were part of the target audience. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?During Year 2, three courses incorporated proteomics instructional material and PD Waterman; (28 students impacted through courses), two faculty members, one staff member, four graduate students (2 PhD and 2 MS) and five undergraduate students, and one high school intern participated in professional development and/or experiential learning opportunities. PD Waterman and graduate students from the proteomics lab trained undergraduates in laboratory basics and hands-on protein isolation and analysis skills, including basic micropipetting skills, protein isolation from cells and tissues, one-dimensional protein gel electrophoresis, imaging and image analysis. How have the results been disseminated to communities of interest?Results have been shared through conference presentations, one dissertation, and one thesis. What do you plan to do during the next reporting period to accomplish the goals?In year three, PD Waterman will participate in 'train-the-trainer' style professional development activities to gain insight from proteomics experts on how to conduct workshops at N.C. A&T in a sustainable manner. For example, there are plans to develop and record proteomics training video material so that interested faculty, staff and students will have access to proteomics training/professional development resources at will via the internet.
Impacts
What was accomplished under these goals?
Access to proteomics expertise and technology continues to be a problem for many academic researchers, particularly those at minority-serving institutions. The goal of this project is to enhance proteomics expertise at North Carolina Agricultural and Technical State University (NCA&T) through proteomics-based professional development and training opportunities for faculty and students. Several members of the proteomics lab received updated training in preparation for training others in Year 2 of the project to incorporate new protein extract clarification methods for low-abundance protein signal improvement. Enhanced access to proteomics expertise and technology at N.C. A&T will help to address the national need for STEM field professionals with proteomics expertise to make sense of the ever increasing number of genome sequences.
Publications
- Type:
Theses/Dissertations
Status:
Awaiting Publication
Year Published:
2016
Citation:
McClendon, C.J. (2016) Biomarkers of Airway Inflammation and Oxidative Stress Regulated by Exposure to Swine Production Environments. (Doctoral Dissertation)
- Type:
Theses/Dissertations
Status:
Awaiting Publication
Year Published:
2016
Citation:
Doldron, M.S. (2016) Evaluation of Natural Antioxidants for Reduction of Organic Dust Mediated Oxidative Stress and Inflammation. (Thesis)
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Progress 09/01/14 to 08/31/15
Outputs
Target Audience:The target audience in year 1 was mainly undergraduate and graduate students and faculty in the disciplines of agriculture, environmental and life sciences at North Carolina A&T State University that will help provide proteomics training to others (i.e., students, faculty and staff) in upcoming years. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Two members of the proteomics lab participated in a hands-on professional development/training activity at the UNC CH School of Pharmacy Proteomics Facility at to learn kinase proteome (kinome) analysis techniques. Existing gel based proteomics skills were enhanced by learning how new protein isolation and purification techniques to prepare samples for quantitative proteomics. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Access to proteomics expertise and technology continues to be a problem for many academic researchers, particularly those at minority-serving institutions. The goal of this project is to enhance proteomics expertise at North Carolina Agricultural and Technical State University (NCA&T) through proteomics-based professional development and training opportunities for faculty and students. During Year 1 of this project one course was enhanced to include proteomics instructional material and PD Waterman and one doctoral studentparticipated in a professional development opportunity at the University of North Carolina atChapel Hill proteomics facility. Several members of the proteomics lab received updated training in preparation for training others in Year 2 of the project. Enhanced access to proteomics expertise and technology at N.C. A&T will help to address the national need for STEM field professionals with proteomics expertise to make sense of the ever increasing number of genome sequences.
Publications
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