Progress 10/01/14 to 09/30/16
Outputs
Target Audience:The target audience for PLRV, PVY, and nematode resistance research is primarily potato breeders, however this work will benefit in the long term potato growers and the larger potato industry by facilitating the incorporation of virus resistance into new potato varieties. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Attend andparticipate in the Annual meeting of the Potato Association of America, in Michigan, July 2016, and the13thAnnual Solanaceae Conference in Davis, California, September, 2016. How have the results been disseminated to communities of interest?Posters reporting results were presented at The Society of Nematologists and the Organization of Nematologist of Tropical America in Montreal, Quebec, Canada. July, 2016, the 13th Annual Solanaceae Conference in Davis, California, September, 2016, and the Parker Field Day in Moscow, Idaho, July, 2016. What do you plan to do during the next reporting period to accomplish the goals?Sequence data from S. sisymbriifolium will continue to be analyzed for genes associated with G. pallida immunity. Additional experiments are underway to evaluate gene expression levels with and without G. pallida infection, and plant response to salicylic acid and jasmonic acid. Two tetraploid populations will continue to be phenotyped and genotyped. Eden x Western Russet segregating for resistance to G. pallida and Yukon Gem x Russet Norkotah for PVY necrotic response.
Impacts
What was accomplished under these goals?
Develop genomic resources in S. sisymbriifolium to study immunity to potato cyst nematode Globodera pallida. Generate sequence data from S. etuberosum to study potato leafroll virus resistance. Generate potato SNP data for two tetraploid populations, one segregating for resistance to G. pallida and the other segregating for necrosis to PVY. This data will be continue to be analyzed to advance potato breeding efforts.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Kuhl, J.C., R.G. Novy, J.L. Whitworth, M.S. Dibble, B. Schneider, and D. Hall (2016) Development of Molecular Markers Closely Linked to the Potato Leafroll Virus resistance gene, Rlretb, for use in marker-assisted selection. American Journal of Potato Research. 93:203-212.
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Progress 10/01/14 to 09/30/15
Outputs
Target Audience:The target audience for PLRV, PVY, and nematode resistance research is primarily potato breeders, however this work will benefit in the long term potato growers and the larger potato industry by facilitating the incorporation of virus resistance into new potato varieties. Changes/Problems:Some Yukon Gem x Russet Norkotah lines were lost due to tissue culture contamination and will have to be replaced. Contamination was not due to human error but related to sterile hood failure, autoclave malfunction, and a thrips infestation. These issues are being addressed to prevent future loss. What opportunities for training and professional development has the project provided?Attended and participated in the Western Section of the American Society of Plant Biologists meeting in Pullman, WA in June 2015. At this conference a poster was presented and numerous seminars attended on the latest research in plant science. I attended and participated in the annual meeting of the Potato Association of America in Portland, ME in July 2014. How have the results been disseminated to communities of interest?A poster was presented at the Western Section of the American Society of Plant Biologists in Pullman, WA in June 2015, titled Using shoot organogenesis to generate tetraploid Solanum sisymbriifolium to produce hybrid triploid seed. The poster reported progress in developing seedless lines of litchi tomato for use as a trap crop in potato. What do you plan to do during the next reporting period to accomplish the goals?Utilizing the recently initiated nested PCR approach for Rysto and Gpa2/Rx1 new pyrosequencing assays will be developed and optimized. Validate these assays using segregating populations of individuals. Four progeny from the Yukon Gem x Russet Norkotah population, including three showing resistance to systemic spread, have been identified for additional hybridizations so that newly generated populations can be evaluated. Screen these populations for response to Potato Virus Y isolate ID20 (strain NE-11).
Impacts
What was accomplished under these goals?
Objective 3. Evaluate priority crop core subsets and other selected germplasm with morphological descriptors, and key agronomic or horticultural traits, such as general adaptation, phenology, and growth potential. Identify accessions with desirable economical traits for multiple location tests and potential release to broaden the genetic base of breeding gene pools. A diverse set of 32 potato lines were evaluated for resistance to Globodera pallida, pale cyst nematode. These lines included Europe and US cultivars as well as breeding lines developed in the US with the goal of incorporating nematode resistance. Two replicated trials were recently completed. Three lines had an average RF (eggs/g soil) of less than 0.2, NY121, A03882-10YN, and Cycloon. Objective 4. Apply molecular marker techniques to assess diversity, detect duplicated accessions, identify taxa that were difficult to classify with morphological characteristics and associated DNA polymorphism with variations of important economical traits in selected crops. A novel series of DNA-based screening protocols are being developed using pyrosequencing to detect resistance gene copy number in tetraploid potato. A protocol has been designed for Ryadg that appears to accurately detect dosage in a limited number of individuals. A new strategy using nested PCR has been initiated for Rysto and Rx1/Gpa2.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Ali, M.C., J.S. Rowley, J.C. Kuhl, S.M Gray, and A.V. Karasev (2014) Evidence of a monogenic nature of the Nz gene conferring resistance against Potato virus Y strain Z (PVYZ) in potato. American Journal of Potato Research.
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