Source: WASHINGTON STATE UNIVERSITY submitted to NRP
VITICULTURAL AND WINEMAKING PRACTICES THAT IMPACT WINE QUALITY AND STABILITY.
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1003663
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Aug 1, 2014
Project End Date
Mar 31, 2019
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
WASHINGTON STATE UNIVERSITY
240 FRENCH ADMINISTRATION BLDG
PULLMAN,WA 99164-0001
Performing Department
Prosser Irrigated Ag Res & Ext Center
Non Technical Summary
The project has three areas of applied research. Evaluate the impact of mechanized viticultural techniques on grape and wine composition. Evaluate how vineyard pest, disease, infection impacts grape and wine composition.Assess the impact of ripening on grape and wine composition.
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
50211312000100%
Knowledge Area
502 - New and Improved Food Products;

Subject Of Investigation
1131 - Wine grapes;

Field Of Science
2000 - Chemistry;
Goals / Objectives
The ability to assess the quality of Washington wine grapes greatly affects viticultural practices. Washington has a bevy of difficulties to overcome to growing quality grapes including: cold winters, low rainfall, short growing seasons, high light intensity, warm to hot summer temperatures, and large diurnal temperature fluctuations during berry development and ripening. Therefore research conducted in other regions may not be applicable to Washington grapes. Additionally, Washington grapes are grown in many different areas which makes understanding the differences between the grape composition from these areas critical information to grape growers and winemakers to make informed decisions about viticultural practices and purchasing decisions.The Washington winemaking industry has been seriously making wine for less than thirty years and lacks critical wine quality data from the various American Viticultural Areas (AVAs). Thirteen AVAs now exist in the state,and since 2004, seven new AVAs have been created. Industry leaderspredictthe acreagewill double in the next ten years. Research into factors that influence the sustainability of the industry by both evaluating methodologies that improve wine and grape quality and assess modern methodologies that are potentially more sustainable is beneficial to maintain and increase the competitiveness of Washington wines in the local and global marketplaces.
Project Methods
Objective 1. The vineyard location as well as access to mechanized pruning devices will be supplied by our industry partner. Assessment of the vineyard parameters will be conducted by Dr. Keller and his staff. The vineyard trial will be divided into replicated blocks in the vineyard where vines will be pruned using mechanical means and compared to the current industry standard of manual spur pruning. The vineyard block will be split into triplicate blocks for the two treatments. Winemakingusing Cabernet Sauvignon lots will initially be conducted onsite at the Prosser Research Winery andat the Wine Science Center located on the TriCities campus. Wines will be produced in triplicate according to winemaking protocols we have carried out for numerous trials (Harbertson and Keller 2012, Casassa et al. 2013a,c). Wine samples will be collected at pressing and on a monthly basis. Samples will be bottled for later evaluation. We analyzed fruit and wine samples for phenolic constituents, including tannin, anthocyanin, large and small polymeric pigment, and total phenolics as described by Harbertson et al. 2003, Harbertson et al. 2002 and color analysis as outlined in Harbertson and Spayd 2006.Objective 2. A field experiment will be conducted with an industry collaborator in a vineyard wherea portion of the fruit isinfected with red blotch virus. My collaborator Naidu Rayapati will be assessing the virus status of the fruit, yield information and vineyard information. The healthy and infected portions of the vineyard will be separated into four replicates. The fruit will be assessed throughout ripening for the concentration of sugar, acid, pigments (where applicable) and at harvest tannins, and amino acidswill beincluded. After harvest the fruit is made into wine at the experimental winery at the Prosser, IAREC. The wines are assessed daily for Brix until dryness and then the residual sugar concentration is measured along with pH, TA and ethanol concentration. Phenolic measurements are carried out at press (extraction end) and at 180 days (aging). Wines are bottled with screw-caps enclosures within the first 3 months of production and stored in the Prosser wine library at 58°F. The wines will be assessed via sensory analysis after 6 months of bottle aging.Objective 3. Vineyards for the selected cultivars will be identified by our commercial collaborator and will be planted on their own roots although it may be impossible to control for many other vineyard variables (spacing, row orientation, age of vineyard etc.). Cabernet Sauvignon, Syrah, and Pinot noir berries will be harvested at three different target Brix (20, 24 and 28) representing commercially unripe, ripe, and overripe fruit and three cultivars that are known to have both commercial relevance and different tannin concentrations (Harbertson et al. 2008) and anthocyanin concentrations. To control for potential impacts on extraction, equal portions of the fruit will be made into wine representing the different harvest points by either chaptalizing or bleeding off and watering back (in an attempt to maintain the same juice to solids ratio). Each will be carried out in triplicate for a total of nine wines per harvest. Sensory will be carried out after 6 months of bottle aging (minimum). Multiple colormetric analysis of the wines will be done as described here (Harbertson and Spayd 2006) including measures of copigmentation, CIE-Lab, anthocyanin concentration and polymeric pigment concentration. Further, we will augment our measures of pigment concentration by utilizing HPLC chromatography of the pigments as described here (Downey and Rochfort 2008). For tannin analysis we will be utilizing protein precipitation and, depending on funding, different chromatography and chemistry techniques to characterize the tannin polymer distribution found in the wines (Casassa et al. 2013). We are also planning to evaluate anthocyanin to tannin ratio in model systems by purifying tannin from cacao (which has relatively uncomplicated tannins) and anthocyanins from red wine.This portion of the work is fairly ambitious and is highly dependent upon the funding amount received and the quality of graduate students in place to carry out the project.

Progress 08/01/14 to 03/31/19

Outputs
Target Audience: Winemakers and grape growers of WA state that produce commercial products. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? 3 MS Student, 1 PhD Student, 4 Undergraduate Student Interns. The PhD student presented posters and oral presentations of her work at the local and national level which is important professional development. How have the results been disseminated to communities of interest? We have presented our results on both a national and local level at meetings with oral and poster style presentations. We have written articles for trade magazines and email blasts through the University communication network (CAHNRS News, Voice of the Vine, Washington Ag Network). What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? For both projects we were able to procure $271,010 for the latest fiscal year and $1,077,497 over the duration of the project that was supported for this Hatch Project. Project 1: We have set up a mechanized pruning trial and have collected fruit and wine chemistry where we have compared several variables including hand pruning, mechanized pruning, and mechanized pruning and thinning. We found that concerns towards converting a vineyard to mechanical pruning are easily allayed, as compositional differences may be mitigated by crop thinning with standard equipment and practices. Canopy measurements indicated that differences in fruit and wine composition were likely due to vine balance, a relationship between vegetative vigor and fruiting, rather than temperature and light. The differences observed in composition after machine pruning primarily included yield and ripening parameters, where more numerous clusters required additional ripening. While in 2015, yields of machine pruned vines were unexpectedly lower than hand-pruned, in 2016 and 2017 the effect was precisely as hypothesized. 2018 data has not been evaluated as of yet. Thinning of machine pruned crops prior to ripening significantly decreased effects on fruit and wine composition, and improved wine color. Overall, machine pruning of Syrah vines demonstrated the potential to support continued growth of the Washington wine industry by increasing production without compromising the product. Sensory analysis of wines produced in 2016 revealed that the wines made from hand pruned and mechanically pruned late harvest were similar to each other whereas the wines made from mechanically pruned and mechanically pruned and thinned vines were similar to each other. The two groupings separated from each other significantly. The wines made from hand pruned and mechanically pruned late harvest were characterized by canned veggies and metallic whereas the wines made from mechanically pruned and mechanically pruned and thinned vines were characterized as floral and sour. The results were largely being driven by sourness and to a lesser degree alcohol concentration. The wines made from hand pruned and mechanically pruned late harvest had similar acidity and ethanol concentrations while the wines made from mechanically pruned and mechanically pruned and thinned vines had similar but greater acidity and lower ethanol. These results are supportive of the hypothesis that mechanical pruning has no impact on the sensory profile of a wine as long as similar harvest criteria are met. We are currently finalizing the remaining data and believe we have consistent enough data to substantiate our results and it appears that mechanically pruning vines and thinning using a mechanical harvester can yield fruit and wine that is similar to vines that are pruned by hand. The results here are quite promising and we are currently discussing the results with the various major wine companies in WA and the WA grape and wine industry groups. There are more than 55,000 acres of wine grapes in Washington state that require pruning, thinning and picking every year. Mechanical harvesting has largely been implemented in Washington state (~80% of acreage) and saves nearly $20 million each year in labor cost. If that same acreage was also converted to mechanical pruning and mechanical thinning another $10 million could be saved in annual labor costs. Project 2: We have carried out our maturity trial over 2 years using three harvest sugar concentrations (20, 24 and 28% sugar) and two different grape varieties (Syrah and Cabernet Sauvignon). Wines were produced at each harvest and portions were adjusted to the later or earlier sugar concentrations by sugar addition or juice removal and dilution. We have found that anthocyanin content of wine at press (~10 days) is the best predictor of total polymeric pigment content (independent of cultivar). The anthocyanins to tannin ratio was found to have no significant impact on any of the measured phenolic variables. From a practical standpoint we attempted to see if our simple measure of fruit anthocyanin content would correlate with polymeric pigment production and we found no significant relationship. In order to get a better understanding of the nature of the changes occurring during wine aging we have developed a modified method for measuring hydrophobicity of phenolic mixtures. We have found that as wines age their hydrophobicity increases. Based upon comparisons of data gathered from purified phenolic standards our initial results suggested that the remaining phenolics in aged wine tended to be smaller. Upon further investigation we think that the loss of hydrophilic anthocyanins and tannins also helps to explain the result. We also observed that cultivar and fruit maturity also impacted wine hydrophobicity. We have published one paper with regard to this work and another is in progress.

Publications


    Progress 10/01/17 to 09/30/18

    Outputs
    Target Audience:Winemakers, grape growers Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?3 MS Student, 1 PhD Student, 4 Undergraduate Student Interns. The PhD student presented posters and oral presentations of her work at the local and national level which is important professional development. How have the results been disseminated to communities of interest?We have presented our results on both a national and local level at meetings with oral and poster style presentations. We have written articles for trade magazines and email blasts through the University communication network (CAHNRS News, Voice of the Vine, Washington Ag Network). What do you plan to do during the next reporting period to accomplish the goals?Continue with the same style of work.

    Impacts
    What was accomplished under these goals? For both projects we were able to procure $271,010 for the latest fiscal year and $1,077,497 over the duration of the project that was supported for this Hatch Project. Project 1: We have set up a mechanized pruning trial and have collected fruit and wine chemistry where we have compared several variables including hand pruning, mechanized pruning, and mechanized pruning and thinning. We found thatconcerns towards converting a vineyard to mechanical pruning are easily allayed, as compositional differences may be mitigated by crop thinning with standard equipment and practices. Canopy measurements indicated that differences in fruit and wine composition were likely due to vine balance, a relationship between vegetative vigor and fruiting, rather than temperature and light. The differences observed in composition after machine pruning primarily included yield and ripening parameters, where more numerous clusters required additional ripening. While in 2015, yields of machine pruned vines were unexpectedly lower than hand-pruned, in 2016 and 2017 the effect was precisely as hypothesized. 2018 data has not been evaluated as of yet. Thinning of machine pruned crops prior to ripening significantly decreased effects on fruit and wine composition, and improved wine color. Overall, machine pruning of Syrah vines demonstrated the potential to support continued growth of the Washington wine industry by increasing production without compromising the product. Sensory analysis of wines produced in 2016 revealed that the wines made from hand pruned and mechanically pruned late harvest were similar to each other whereas the wines made from mechanically pruned and mechanically pruned and thinned vines were similar to each other. The two groupings separated from each other significantly. The wines made from hand pruned and mechanically pruned late harvest were characterized by canned veggies and metallic whereas the wines made from mechanically pruned and mechanically pruned and thinned vines were characterized as floral and sour. The results were largely being driven by sourness and to a lesser degree alcohol concentration. The wines made from hand pruned and mechanically pruned late harvest had similar acidity and ethanol concentrations while the wines made from mechanically pruned and mechanically pruned and thinned vines had similar but greater acidity and lower ethanol. These results are supportive of the hypothesis that mechanical pruning has no impact on the sensory profile of a wine as long as similar harvest criteria are met. We are currently finalizing the remaining data and believe we have consistent enough data to substantiate our results and it appears that mechanically pruning vines and thinning using a mechanical harvester can yield fruit and wine that is similar to vines that are pruned by hand. The results here are quite promising and we are currently discussing the results with the various major wine companies in WA and the WA grape and wine industry groups. There are more than 55,000 acres of wine grapes in Washington state that require pruning, thinning and picking every year. Mechanical harvesting has largely been implemented in Washington state (~80% of acreage) and saves nearly $20 million each year in labor cost. If that same acreage was also converted to mechanical pruning and mechanical thinning another $10 million could be saved in annual labor costs. Project 2: We have carried out our maturity trial over 2 years using three harvest sugar concentrations (20, 24 and 28% sugar) and two different grape varieties (Syrah and Cabernet Sauvignon). Wines were produced at each harvest and portions were adjusted to the later or earlier sugar concentrations by sugar addition or juice removal and dilution. We have found that anthocyanin content of wine at press (~10 days) is the best predictor of total polymeric pigment content (independent of cultivar). The anthocyanins to tannin ratio was found to have no significant impact on any of the measured phenolic variables. From a practical standpoint we attempted to see if our simple measure of fruit anthocyanin content would correlate with polymeric pigment production and we found no significant relationship. In order to get a better understanding of the nature of the changes occurring during wine aging we have developed a modified method for measuring hydrophobicity of phenolic mixtures. We have found that as wines age their hydrophobicity increases. Based upon comparisons of data gathered from purified phenolic standards our initial results suggested that the remaining phenolics in aged wine tended to be smaller. Upon further investigation we think that the loss of hydrophilic anthocyanins and tannins also helps to explain the result. We also observed that cultivar and fruit maturity also impacted wine hydrophobicity.We have published one paper with regard to this work and another is in progress.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Sherman, E., J.F. Harbertson, D.R. Greenwood, S.G. Villas-Boas, and H. Heymann. 2018. Reference samples guide variable selection for correlation of wine sensory and volatile profiling data. Food Chemistry. 30:344-354.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Merrell, C.P., R.C. Larsen, and J.F. Harbertson. 2018. Effects of berry maturity and wine alcohol on phenolic content during winemaking and aging. Am. J. Enol. and Vitic. 69:1-11.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Liu, J., W.L.P. Bredie, E. Sherman, J.F. Harbertson and H. Heymann. 2018. Comparison of rapid descriptive sensory methodologies: Free choice profiling, flash profile and modified flash profile. Food Research International. 106:892-900.
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Merrell, C., and J.F. Harbertson. Impact of Fruit Maturity and Extended Maceration on Phenolic Extraction of Cabernet Sauvignon Wines. Am. Soc. of Enol. Vitic. 69th Annual Meeting, Monterey, CA (http://www.asev.org/sites/main/files/file-attachments/2018technicalabstracts.pdf) pg 51-52
    • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Williams, A.T, T. Henick-Kling, H. Piao, J.F. Harbertson, T.S. Collins, C. Merrell, R. Larsen, M. Williamson, D. Seed, and Cary Wilton. 2018. Managing pH and Acid Composition to Assess Microbial Ecology of Wine Fermentation. Am. Soc. of Enol. Vitic. 69th Annual Meeting, Monterey, CA (http://www.asev.org/sites/main/files/file-attachments/2018technicalabstracts.pdf) pg 80
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Fox, D. and J.F. Harbertson. 2018 Impact of Vineyard Site and Clone on Phenolic Composition of Cabernet Sauvignon Wines. Am. Soc. of Enol. Vitic. 69th Annual Meeting, Monterey, CA (http://www.asev.org/sites/main/files/file-attachments/2018technicalabstracts.pdf) pg 93-94
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: American Society of Enology and Viticulture 2018 Enology Best Paper, Impact of Grape Maturity and Ethanol Concentration on Sensory Properties of Washington State Merlot Wines, by Emma Sherman, David R. Greenwood, Silas G. Villas-Bo�s, Hildegarde Heymann and James F. Harbertson. 69th Annual Meeting, Monterey, CA
    • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Harbertson, J.F. Alcohols Influence on Sensory Development Oregon Wine Symposium Portland Oregon, February 20, 2018
    • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Harbertson, J.F. Advancing the art of winemaking with science: Impact of grape ripening on wine phenolics and sensory attributes. Show Me Grape and Wine Conference, Columbia Missouri, March 8, 2018
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Harbertson, J.F. Ripening Experiments in Washington State. Virginia Winery Association Annual Meeting and Conference, Wintergreen Virginia November 9, 2018
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2018 Citation: Harbertson, J.F. Impact of Grape Ripening on Wine Phenolics and Sensory Attributes. Virginia Winery Association Annual Meeting and Conference, Wintergreen Virginia November 9, 2018


    Progress 10/01/16 to 09/30/17

    Outputs
    Target Audience:Grape and Wine Industry Members:Grape growers, winemakers, vineyard managers, cellar workers Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?1 MS Student, 1 PhD Student, 4 Undergraduate Student Interns. The Dissertation and Thesis are listed earlier. Students have also presented their work nationally and internationally. How have the results been disseminated to communities of interest?We have presented our results on both a national and local level at meetings with oral and poster style presentations. We have written articles for trade magazines and email blasts through the University communication network (CAHNRS News, Voice of the Vine, Washington Ag Network).? Information and project result dissemination, and stakeholder training and development, are outlined below. The PI has given several talks at the local "Grape Technical Group" as it relates to canopy manipulation and disease management. Various articles relating to vineyard management, and pest management, were published in the Viticulture and Enology Extension Newsletter (Spring and Fall 2017). The PI also presented at a special topics workshop on disease management at the 2017 American Society for Enology and Viticulture annual meeting. Overall, communities of interest for the WSU Viticulture Research and Extension programs are reached through our use of multiple media types. This includes, but is not limited to, email blasts, Facebook, YouTube, Twitter, biannual Newsletters, website articles, Field Days, radio and newspaper interviews. Multiple research posters with an Extension-bend were presented at various grower-based meetings in 2016-2017 (Washington WineGrowers Association Washington State Grape Society, field Days in June, and August). What do you plan to do during the next reporting period to accomplish the goals?Continue working on the same goals

    Impacts
    What was accomplished under these goals? For both projects we were able procure $243,847 for the latest fiscal year and $806,487 over the duration of the project that was supported for this Hatch Project. Project 1: We have set up a mechanized pruning trial and have collected fruit and wine chemistry where we have compared several variables including hand pruning, mechanized pruning, and mechanized pruning and thinning.?We found that that concerns towards converting a vineyard to mechanical pruning are easily allayed, as compositional differences may be mitigated by crop thinning with standard equipment and practices. Canopy measurements indicated that differences in fruit and wine composition were likely due to vine balance, a relationship between vegetative vigor and fruiting, rather than temperature and light. The differences observed in composition after machine pruning primarily included yield and ripening parameters, where more numerous clusters required additional ripening. While in 2015, yields of machine pruned vines were unexpectedly lower than hand-pruned, in 2016 and 2017 the effect was precisely as hypothesized. Thinning of machine pruned crops prior to ripening significantly decreased effects on fruit and wine composition, and improved wine color. Overall, machine pruning of Syrah vines demonstrated the potential to support continued growth of the Washington wine industry by increasing production without compromising the product. Wines are undergoing sensorial analysis. Although we don't have three years of consistent data to substantiate our results it appears that mechanically pruning vines and thinning using a mechanical harvester can yield fruit and wine that is similar to vines that are pruned by hand.?The results here are quite promising and we are currently discussing the results with the various major wine companies in WA and the WA grape and wine industry groups. There are more than 55,000 acres of wine grapes in Washington state that require pruning, thinning and picking every year. Mechanical harvesting has largely been implemented in Washington state (~80% of acreage) and saves nearly $20 million each year in labor cost. If that same acreage was also converted to mechanical pruning and mechanical thinning another $10 million could be saved in annual labor costs. Project 2: We have carried out our maturity trial over 2 years using three harvest sugar concentrations (20, 24 and 28% sugar) and two different grape varieties (Syrah and Cabernet Sauvignon). Wines were produced at each harvest and portions were adjusted to the later or earlier sugar concentrations by sugar addition or juice removal and dilution. We have found that anthocyanin content of wine at press (~10 days) is the best predictor of total polymeric pigment content (independent of cultivar). The anthocyanins to tannin ratio was found to have no significant impact on any of the measured phenolic variables. From a practical standpoint we attempted to see if our simple measure of fruit anthocyanin content would correlate with polymeric pigment production and we found no significant relationship. In order to get a better understanding of the nature of the changes occurring during wine aging we have developed a modified method for measuring hydrophobicity of phenolic mixtures. We have found that as wines age their hydrophobicity increases. Based upon comparisons of data gathered from purified phenolic standards our initial results suggested that the remaining phenolics in aged wine tended to be smaller. Upon further investigation we think that the loss of hydrophilic anthocyanins and tannins also helps to explain the result. We also observed that cultivar and fruit maturity also impacted wine hydrophobicity. In the fiscal reporting year, HATCH funds enabled Co-PI Moyer to procure an additional $252,370 in funding from other state and federal sources to support research in Grapevine Crown Gall, Grapevine Powdery Mildew Fungicide Resistance, General vineyard IPM, Vineyard Nematode Management, and Grapevine rootstock evaluation trials directly for her program. It also allowed her to collaborate on state and federal projects, whose combined value was over $8M.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Frost, S.C., J.F. Harbertson, and H. Heymann. 2017. A full factorial study on the effect of tannins, acidity, and ethanol on the temporal perception of taste and mouthfeel in red wine. Food Quality and Preference. 62:1-7
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Sherman, E., D.R. Greenwood, S.G. Villas-Boas, H. Heymann, and J.F. Harbertson. 2017. Impact of grape maturity and ethanol concentration on sensory properties of Washington State Merlot wines. Am. J. Enol. and Vitic. 68:344-356
    • Type: Journal Articles Status: Awaiting Publication Year Published: 2017 Citation: Merrell, C.P. and J.F. Harbertson. 2017. Sulfur dioxide management during aging is an important factor for the development of Rose wine color. Catalyst: Discovery into Practice�DOI:�10.5344/catalyst.2017.17003
    • Type: Journal Articles Status: Awaiting Publication Year Published: 2018 Citation: Sherman, E., J.F. Harbertson, D.R. Greenwood, S.G. Villas-Boas, and H. Heymann. 2018. Reference samples guide variable selection for correlation of wine sensory and volatile profiling data. Food Chemistry. doi.org/10.1016/j.foodchem.2017.10.073
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: J.F. Harbertson. Hang time experiments in Washington State. Wine of Western Australia Cabernet Sauvignon Forum in Margaret River, Curtin University, January 24, 2017
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: J.F. Harbertson. The Washington Wine Industry. Wine of Western Australia Cabernet Sauvignon Forum in Margaret River, Curtin University, January 24, 2017
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: J.F. Harbertson. Modern Winemaking. Climate Extremes: Is the Pacific Northwest Wine Industry Ready? Ravenholt Lecture Series, Washington State University Richland, March 17, 2017.
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: J.F. Harbertson. Ripening and Sensory Impacts of Regulated Deficit Irrigation. WIGA Viticulture and Oenology Conference, Duncan Victoria Canada, April 25, 2017
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: J.F. Harbertson. Wine Phenolics - How They Change during Ripening, Winemaking, and Aging. WIGA Viticulture and Oenology Conference, Duncan Victoria Canada, April 25, 2017
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Casassa, F., R.C. Larsen and J.F. Harbertson. Effects of Vineyard and Winemaking Practices Impacting Berry Size on Evolution of Phenolics during Winemaking?. American Society for Enology and Viticulture National Conference Bellevue Washington, June 29, 2017
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Merrell, C., and J.F. Harbertson. Impact of Berry Maturity and Alcohol Content on Wine Phenolic Hydrophobicity and Content Over Time. American Society for Enology and Viticulture National Conference Bellevue Washington, June 29, 2017
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Conference Poster: Merrell, C. and J.F. Harbertson. Isolation and purification of Vitis vinifera grape and wine anthocyanins and tannins. Washington Winegrowers 2017 Convention, Kennewick Washington February 9, 2017
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Conference Poster: Merrell, C., J.F. Harbertson and J.F. Harbertson. Evaluation of the hydrophobicity of whiskies and UHPLC-QTOF/MS characterization of their water and octanol phases. Worldwide Distilled Spirits Conference, Glasgow, Scotland, May 29, 2017
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: Conference Poster: Collins, T., C. Merrell, and J.F. Harbertson. Comparison of Bourbon and Scotch Whiskies Using Phenolic Hydrophobicity and UHPLC-QTOF/MS. American Society for Enology and Viticulture National Conference, Bellevue Washington, June 28, 2017
    • Type: Journal Articles Status: Accepted Year Published: 2017 Citation: Gale, E.J. and M.M. Moyer. 2017. Cold Hardiness of Vitis vinifera Roots. Am. J. Enol. Vitic. 68: 468-477.
    • Type: Journal Articles Status: Accepted Year Published: 2017 Citation: Moyer, M.M., A.N. Boren, and J. Tarara. 2017. Dual Fumigant and Herbicide Use Optimizes Replanting Preparation in a Virus and Nematode-Affected Vineyard. Catalyst: Discovery into Practice. 1:55-61.
    • Type: Journal Articles Status: Accepted Year Published: 2017 Citation: Ricketts, K.D., M.I. Gomez, M.F. Fuchs, T.E. Martinson, R.H. Smith, M.L. Cooper, M.M. Moyer and A. Wise. 2017. Estimating the Economic Impact of Grapevine Red Blotch Disease in California, New York and Washington, and Developing Optimal Management Strategies. Am. J. Enol. Vitic. 68: 127-135
    • Type: Theses/Dissertations Status: Accepted Year Published: 2017 Citation: Dissertation Merrell, C.P. Phenolic Evolution in wine: Determination of new methodology to measure phenolic content and the impact of maturity, alcohol, and sulfur dioxide on wine color during aging.
    • Type: Other Status: Accepted Year Published: 2017 Citation: Hoheisel, G.A. and M.M. Moyer. (eds). Pest Management Guide for Grapes in Washington.�(Updated Annually). WSU Extension Publication #EB0762. Washington State University.
    • Type: Other Status: Accepted Year Published: 2017 Citation: Moyer, M.M., and C. Cooper. 2017. Wholesale Buyers Guide to Washington Grapevine Quarantines. WSU Extension Publishing
    • Type: Theses/Dissertations Status: Accepted Year Published: 2016 Citation: Thesis Jenkins, C.A. COMPARISON OF GRAPE AND WINE COMPOSITION, YIELD, AND CELL WALL BINDING POTENTIAL OF GRAPES FROM MECHANICALLY PRUNED AND HAND PRUNED SYRAH VINES
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: J.F. Harbertson. Phenolics of Washington State wines and grapes. Washington Winegrowers 2017 Convention, Kennewick Washington February 9, 2017
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: J.F. Harbertson. Impacts of Grape Maturity on Wine Color, Sensory Attributes. Washington Advancements in Viticulture and Enology, April 19, 2017
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: J.F. Harbertson, Methods for Measuring Wine Color, American Society for Enology and Viticulture National Conference Bellevue Washington, June 28, 2017
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2017 Citation: .F. Harbertson. Managing Phenolics in the winery focus on pigments and tannins. Washington Advances in Viticulture and Enology Extension Seminar, Woodinville and Walla Walla Washington July 11 and July 17 2017.


    Progress 10/01/15 to 09/30/16

    Outputs
    Target Audience:Washington Wine Grape Growers, Washington Winemakers, US Wine Grape Growers (Primarily CA), US Winemakers Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?1 MS Student, 1 PhD Student, 3 Undergraduate Student Interns have participated on the project. The PhD student presented posters and oral presentations of her work at the local and national level which is important professional development. For Dr. Moyer who joined the project between 8/01/2016 - 9/30/2016 One MS student (nematode sampling, identification, pest management) and 1 PhD candidate (disease management, sprayer calibration) were partially trained during the reporting period, along with 1 undergraduate intern (pest management, vineyard replanting, nematode sampling, pesticide education). How have the results been disseminated to communities of interest?We have presented our results on both a national and local level at meetings with oral and poster style presentations. We have written articles for trade magazines and email blasts through the University communication network (CAHNRS News, Voice of the Vine etc.). For Dr. Moyer: WA State Viticulture Field Day (Aug 12), Viticulture and Enology Extension News (Newsletter, Sept 15th). Additional information through radio interviews, Facebook, website articles, email blasts through a maintained listserv, trade publication interviews. What do you plan to do during the next reporting period to accomplish the goals?We anticipate publishing our results for the wine and viticulture results in journals that specialize in that area of publishing (American Journal of Enology and Viticulture, Australian Journal of Grape and Wine Research) and we intend to extend those results into either the WSU Viticulture and Enology Newsletter or WSU extension publications. The results will also be shared at the local wine grape grower meeting (WAWGG) and the Wine Technical Group through posters or presentations and at the national level at the American Society of Enology and Viticulture National Conference. The more technical chemical methodology work will likely be published in journals that are more technical in nature such as Journal of Chromatography,Food Chemistry or the Journal of Agriculture and Food Chemistry

    Impacts
    What was accomplished under these goals? Project 1: We have set up a mechanized pruning trial and have collected fruit and wine chemistry where we have compared several variables including hand pruning, mechanized pruning, and mechanized pruning and thinning. We found that that concerns towards converting a vineyard to mechanical pruning are easily allayed, as compositional differences may be mitigated by crop thinning with standard equipment and practices. Canopy measurements indicated that differences in fruit and wine composition were likely due to vine balance, a relationship between vegetative vigor and fruiting, rather than temperature and light. The differences observed in composition after machine pruning primarily included yield and ripening parameters, where more numerous clusters required additional ripening. While in 2015, yields of machine pruned vines were unexpectedly lower than hand-pruned, in 2016 the effect was precisely as hypothesized. Thinning of machine pruned crops prior to ripening significantly decreased effects on fruit and wine composition, and improved wine color. Overall, machine pruning of Syrah vines demonstrated the potential to support continued growth of the Washington wine industry by increasing production without compromising the product. Although we don't have three years of data to substantiate our results it appears that mechanically pruning vines and thinning using a mechanical harvester can yield fruit and wine that is similar to vines that are pruned by hand. Project 2: We have carried out our maturity trial over 2 years using three harvest sugar concentrations (20, 24 and 28% sugar) and two different grape varieties (Syrah and Cabernet Sauvignon). Wines were produced at each harvest and portions were adjusted to the later or earlier sugar concentrations by sugar addition or juice removal and dilution. We have found that anthocyanin content of wine at press (~10 days) is the best predictor of total polymeric pigment content (independent of cultivar). The anthocyanins to tannin ratio was found to have no significant impact on any of the measured phenolic variables. From a practical standpoint we attempted to see if our simple measure of fruit anthocyanin content would correlate with polymeric pigment production and we found no significant relationship. In order to get a better understanding of the nature of the changes occurring during wine aging we have developed a modified method for measuring hydrophobicity of phenolic mixtures. We have found that as wines age their hydrophobicity increases. Based upon comparisons of data gathered from purified phenolic standards our initial results suggested that the remaining phenolics in aged wine tended to be smaller. Upon further investigation we think that the loss of hydrophilic anthocyanins and tannins also helps to explain the result. We also observed that cultivar and fruit maturity also impacted wine hydrophobicity.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Casassa, L.F., R.C. Larsen, and J.F. Harbertson. 2016. Effects of vineyard and winemaking practices impacting berry size on evolution of phenolics during winemaking. Am. J. Enol. Vitic. doi: 10.5344/ajev.2016.15105
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Sommer, S.C., Dickenscheid, J.F. Harbertson, U. Fischer, and S.D. Cohen. 2016. Rationale for haze formation after carboxymethyl cellulose addition to red wine. J. Agric. Food Chem. 10.1021/acs.jafc.6b02479
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Keller, M., P. Romero, H. Gohil, R.P. Smithyman, W.R. Riley, L.F. Casassa and J.F. Harbertson. 2016. Deficit irrigation alters grapevine growth, physiology and fruit microclimate. Am. J. Enol. doi: 10.5344/ajev.2016.16032
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Alabi, O.J., L.F. Casassa, L.R. Gutha, R.C. Larsen, T.H-Kling, J.F. Harbertson and R.A. Naidu. Impacts of grapevine leafroll disease on fruit yield and grape and wine chemistry in a wine grape (Vitis vinifera L.) cultivar. Plos One. dx.doi.org/10.1371/journal.pone.0149666
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Beaver, C.W., M. Mireles, and J.F. Harbertson. 2016. Comparison of multivariate regression methods for the analysis of phenolics in wine made from two Vitis vinifera cultivars. Am. J. Enol. Vitic. 67:56-64.
    • Type: Other Status: Published Year Published: 2016 Citation: Casassa L.F., and J.F. Harbertson. 2016, Studying the relationships between seed maturity, length of maceration and ethanol amount on Merlot wines. Practical Winery and Vineyard. September pp. 55-59.
    • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Sherman, E., D.R. Greenwood, S.G. Villas-B�as, H. Heymann, J. F. Harbertson, O. Fiehn. 2016. A flavoromics approach to investigating relationships between wine sensory properties and volatile profiles. 1st Food Chemsitry Conference, Amsterdam, Netherlands. URL: (http://www.foodchemistryconference.com/conference-program.asp)
    • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Merrell, C., J.F. Harbertson. 2016. Impact of Cabernet Sauvignon berry maturity on wine anthocyanin, tannin, and polymeric pigment content over time. Am. Soc. of Enol. Vitic. 67th Annual Meeting, Monterey, CA. URL: (http://www.asev.org/sites/main/files/file-attachments/2016technicalabstracts.pdf) pg 64
    • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Zhang, Y., L. Rocchi, J.F. Harbertson, M.Keller. 2016. Irrigation strategies for white winegrape production. Am. Soc. of Enol. Vitic. 67th Annual Meeting, Monterey, CA. URL: (http://www.asev.org/sites/main/files/file-attachments/2016technicalabstracts.pdf) pg 70
    • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Harbertson, J.F. Managing phenolics: interaction between vineyard and winemaking treatments. 2016. 9th International Cool Climate Wine Symposium, Brighton, UK.


    Progress 10/01/14 to 09/30/15

    Outputs
    Target Audience:Wine grape growers, winemakers, food and agricultural scientists Changes/Problems:We have not had any significant delays in the project however given the results from the mechanical pruning trial we may need to repeat the experiment again. What opportunities for training and professional development has the project provided?1 MS Student, 1 PhD Student, 2 Undergraduate Student Interns have participated on the project. The PhD student presented posters and oral presentations of her work at the local and national level which is important professional development. How have the results been disseminated to communities of interest?We have presented our results on both a national and local level at meetings with oral and poster style presentations. We plan on writing articles for trade magazines and email blasts through the University communication network (CAHNRS News, Voice of the Vine etc.). What do you plan to do during the next reporting period to accomplish the goals?We anticipate publishing our results for the wine and viticulture results in journals that specialize in that area of publishing (American Journal of Enology and Viticulture, Australian Journal of Grape and Wine Research) and we intend to extend those results into either the WSU Viticulture and Enology Newsletter or WSU extension publications. The results will also be shared at the local wine grape grower meeting (WAWGG) and the Wine Technical Group through posters or presentations and at the national level at the American Society of Enology and Viticulture National Conference. The more technical chemical methodology work will likely be published in journals that are more technical in nature such as Journal of Chromatography, Food Chemistry or the Journal of Agriculture and Food Chemistry.

    Impacts
    What was accomplished under these goals? We have set up the mechanized pruning trial and have collected fruit and wine chemistry. Likewise we have set up our maturity trial and collected both wine and fruit data. We have also been successful in our development of the purification methodologies for anthocyanins and tannins. We have purified tannins and anthocyanins from the wines made in year 2 and are in the process of evaluating the model solutions and wines. In the first year we found that when comparing mechanical and hand pruning vines the mechanically pruned vines had significantly greater yield and less ripe fruit, which resulted in wines that had significantly lower concentrations of ethanol and phenolics. In the second year we found that if we mechanically thinned the mechanically pruned fruit to an amount comparable to the hand pruned vineyard in the previous year we had significantly greater amounts of phenolics and ethanol than the mechanical and hand pruned vineyard treatments. We have harvested Cabernet Sauvignon and Syrah fruit from three different maturities and made wines as outlined in our original proposal and the wines have significantly different amounts of anthocyanins and tannins. We have devised novel purification techniques for the purification of anthocyanins and tannins.

    Publications

    • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Casassa, L.F., M. Keller, and J.F. Harbertson. 2015. Regulated deficit irrigation alters anthocyanins, tannins and sensory properties of Cabernet Sauvignon grapes and wine. Molecules. 20:7820-7844.
    • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Harbertson, J.F., M. Mireles, and Y. Yue. 2015. Improvement of BSA tannin precipitation assay by reformulation of resuspension buffer. Am. J. Enol. Vitic. 66:95-99.
    • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: Interaction of tannin, acid, and ethanol concentration on the temporal perception of taste and mouthfeel American Society of Enology and Viticulture 2015 National Conference, June 15-18
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Conference Abstract and Poster: Comparison of Multivariate Regression Methods for the Analysis of Phenolics in Red Wine American Society of Enology and Viticulture 2015 National Conference, June 15-18
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Conference Abstract and Oral Presentation: Interaction of tannin, acid, and ethanol concentration on the temporal perception of taste and mouthfeel Scott Frost, Richard Larsen, James Harbertson, Hildegarde Heymann American Society of Enology and Viticulture 2015 National Conference, June 15-18
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Conference Poster: Comparison of Multivariate Regression Methods for the Analysis of Phenolics in Red Wine Christopher W. Beaver, Maria S. Mireles, and James Harbertson* American Society of Enology and Viticulture 2015 National Conference, June 15-18
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Conference Abstract and Poster: Isolation and Purication of Vitis vinifera Grape and Wine Anthocyanins and Tannins Caroline Merrell and James F. Harbertson* American Society of Enology and Viticulture 2015 National Conference, June 15-18
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Conference Abstract and Poster: Impact of Grape Maturity and Ethanol Concentration on the Composition of Washington State Merlot Wines Emma Sherman,* David R. Greenwood, Silas G. Villas-B�as, Oliver Fiehn, Hildegarde Heymann, and James F. Harbertson
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Regional Differences between Typical Riesling Wines from Rheingau and Washington State Kimmo Sir�n,* James F. Harbertson, Armin Sch�ttler, Rainer Jung, Claus-Dieter Patz, Thomas Henick-Kling, and Doris Rauhut
    • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: Comparison of Multivariate Regression Methods for the Analysis of Phenolics in Wine Made from Two Vitis vinifera Cultivars Christopher W. Beaver and James F. Harbertson* Am. J. Enol. Vitic. 67:1 (2016)
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Conference Presentation: Actionable Research: Phenolics in grapes and wine at Innovation Quality, Charles Krug Winery, St. Helena CA May 4, 2015
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Conference Presentation:Balancing Wine Tannins in a Warm Climate Outreach Seminar Balancing Vine and Wines from Coast to Desert American Society of Enology and Viticulture National Conference June 18, 2015
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Conference Presentation: Phenolics and their relationship to weather and ripeness Washington Association of Wine and Grape Growers Convention, Kennewick WA, Feb 10-12 2015
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Conference Presentation: Overview of tannin extraction & evolution during fermentation and aging Washington Association of Wine and Grape Growers Convention, Kennewick WA, Feb 10-12 2015
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Isolation and Purication of Vitis vinifera Grape and Wine Anthocyanins and Tannins Caroline Merrell and James F. Harbertson*
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Conference Poster: Isolation and Purication of Vitis vinifera Grape and Wine Anthocyanins and Tannins Caroline Merrell and James F. Harbertson* Washington Association of Wine and Grape Growers Convention, Kennewick WA, Feb 10-12 2015


    Progress 08/01/14 to 09/30/14

    Outputs
    Target Audience: Winemakers and grape growers of WA state that produce commercial products. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? We havemade presentations about the impact of grape maturity at a National meeting for grape and wine professionals. What do you plan to do during the next reporting period to accomplish the goals? In the coming year we will be presenting results at winemaker and grape grower meetings as well as WSU extension events.

    Impacts
    What was accomplished under these goals? We have successfully procured a grant to carry out our goals: 1) to evaluate the impacts of mechanized pruning on grape and wine composition and 2) evaluate the impact of maturity on phenolics. Thus far we have set up the mechanized pruning trial and have collected 1-year of fruit and wine chemistry data. Likewise, we have set up our maturity trial and collected 1-year of wine and fruit data. We have also been successful in our development of the purification methodologies for anthocyanins and tannins that was set out in objective 2. We are also in the process of developing a new tannin separation method that is based on their molecular weight.

    Publications

    • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Spayd, S.E., J.F. Harbertson and M.S. Mireles. 2014. Concentrations of phenolic components in North Carolina Wines. J. Food Chem. Nutr. 2:93-102.
    • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Casassa, L.F., and J.F. Harbertson. 2014. Extraction, evolution and sensory impact of phenolic compounds during red wine maceration. Annual Review of Food Science and Technology 5:83-109.
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2014 Citation: Harbertson, J.F., G.P. Parpinello, H. Heymann, M.O. Downey. 2014. Tannin addition use in the production of red and white wines. Australian Society of Viticulture and Oenology, Adelaide
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2014 Citation: L.F. Casassa, R.C. Larsen, M.S. Mireles, J.F. Harbertson. 2014. Effect of regulated deficit irrigation on saign�e treatments of Cabernet Sauvignon must. Am. Soc. of Enol. Vitic. 65th Annual Meeting, Austin, URL: (http://www.asev.org/sites/main/files/file-attachments/2014technicalabstracts.pdf) pg 75
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2014 Citation: J.F. Harbertson, R.C. Larsen, and M.S. Mireles. 2014. Impacts of grape maturity and ethanol concentration on wine tannin concentration. Am. Soc. of Enol. Vitic. 65th Annual Meeting, Austin, URL: (http://www.asev.org/sites/main/files/file-attachments/2014technicalabstracts.pdf) pg. 98
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2014 Citation: J.F. Harbertson, R.L. Kilmister, M.A. Kelm. 2014. Impact of individual and mixed condensed tannin polymers on their ability to precipitate protein. Am. Soc. of Enol. Vitic. 65th Annual Meeting, Austin, URL: (http://www.asev.org/sites/main/files/file-attachments/2014technicalabstracts.pdf) pg. 106
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2014 Citation: S.E. Spayd, J.F. Harbertson, M.S. Mireles. 2014. Survey of phenolics in North Carolina Wines. URL: (http://www.asev.org/sites/main/files/file-attachments/2014technicalabstracts.pdf) pg. 124
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2014 Citation: H.L. Gohil, P. Romero, G. Carmassi, J.F. Harbertson and M. Keller. 2014. Determining the effect of regulated deficit irrigation on Cabernet Sauvignon in Washington. URL: (http://www.asev.org/sites/main/files/file-attachments/2014technicalabstracts.pdf) pg. 158
    • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Harbertson, J.F., R.L. Kilmister, M.A. Kelm, and M.O. Downey. 2014. Impact of condensed tannin size and mixed polymers on bovine serum albumin precipitation. Food Chem. 160:16-21.