Source: UNIVERSITY OF FLORIDA submitted to
IMPROVED EX SITU CULTURE SYSTEM FOR ACROPORA CORALS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
1003491
Grant No.
(N/A)
Project No.
FLA-FOR-005358
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Jul 28, 2014
Project End Date
Jun 30, 2016
Grant Year
(N/A)
Project Director
Flint, MA.
Recipient Organization
UNIVERSITY OF FLORIDA
G022 MCCARTY HALL
GAINESVILLE,FL 32611
Performing Department
Forest Resources and Conservation
Non Technical Summary
Coral reef restoration research has focused on in situ "gardens" located in coastal waters for adult grow out (Boch and Morse 2012, Ecol Eng; Young et al. 2012, Bull Mar Sci). However, researchers have identified the potential for ex situ culture systems to provide benefits to coral conservation efforts (Hagedorn et al. 2009, Proc Smithsonian Mar Sci Symp). These benefits include housing of genetic stocks in areas not affected by stressors and disturbances, ability to closely monitor coral condition, and experimental control of abiotic parameters for genotype testing. We propose to design and build a recirculating system for coral culture. This system will be located in a greenhouse at the Center for Conservation facility at Apollo Beach.
Animal Health Component
10%
Research Effort Categories
Basic
0%
Applied
50%
Developmental
50%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
11108991050100%
Goals / Objectives
Development of a resource efficient land-based system for rearing federally endangered corals utilized in reef restoration.
Project Methods
This project will involve design and construction of recirculating aquaculture systems (RASs) designed specifically for culturing colonies of the important reef-building scleractinians, staghorn coral Acropora cervicornis and elkhorn coral Acropora palmata. Once the RASs are functional, general scientific methods will include testing of fixed effects such as flow regime, water depth, light exposure, supplemental feeding with zooplankton, etcetera utilizing coral health and growth as random effects. Experiments such as these will allow us to optimize parameters for ex situ culture. The unique aspect of this work will be our ability to take advantage of the sessile nature of coral colonies. When performing controlled studies on finfish or other motile aquatic animals, it is always necessary to design RASs with enough separate tanks that sufficient replication can be achieved within each treatment level. However, the life history of adult corals enables us to design systems with a relatively small number of large, shallow tray-type tanks. Because each colony can be uniquely marked, and its performance measured quantitatively, all replicates of a given treatment level can be combined within a single tank. This also serves to minimize confounding effects of intra-treatment variation that occur when replicates have to be maintained in separate tanks. Employing RASs engenders a level of controlled experimentation which permits relatively simple statistical analyses. Analysis of variance or repeated measures analysis of variance with a priori tests such as REGWQ will describe differences between the fixed effects being tested.Efforts which will be utilized to deliver science-based knowledge through our RASs will take the form of visitor outreach by University of Florida personell at the Florida Aquarium's Center for Conservation (CFC). The CFC will be a part of the Florida Conservation and Technology Center (FCTC), which is a joint venture of Tampa Electric Company, The Florida Fish and Wildlife Conservation Commission, and The Florida Aquarium. FCTC will contain a range of outdoor activities and educational experiences related to conservation of Florida's natural resources. We envision a tour of our coral research greenhouse as part of the FCTC experience for visitors interested in gaining an understanding of the hands-on research University of Florida scientists are conducting at CFC. Experiential learning opportunities will also be plentiful for volunteers who might work alongside scientists and graduate students in the coral research greenhouse. Interest in the project is high, as we have already had Florida Aquarium volunteers participating in greenhouse construction.Success of this project will ultimately be evaluated within two broad categories: (1) New knowledge to optimize of ex situ culture of A. cervicornis and A. palmata and (2) Public outreach which increases science-knowledge of coral aquaculture.New knowledge to optimize of ex situ culture of A. cervicornis and A. palmataAbility to statistically differentiate coral performance across fixed effect treatment levels.Coral performance to include linear growth, mass increase, Symbiodinium recruitment, bleaching, and disease occurrence.Key quantitative indicator of success will be the ultimate ability to combine best performing treatment levels from multiple experiments (flow regime, water depth, light exposure, etc.) to measurably improve growth and health of Acropora corals in RASs versus current practices.Development of a "Best Management Practices" document for Acropora corals in RASs.Public outreach which increases science-based knowledge of coral aquacultureDevelopment of an effective educational message for visitors through interaction and assessment of visitor understanding.Acquisition of new skills and conceptual understanding for volunteers working with RASs in the coral greenhouse.Accomplishment of these indicators of success will fulfill the expected project outcomes in terms of an improved ability to grow Acropora corals in land-based culture systems. In addition, expected project impacts of increasing public awareness, appreciation, and knowledge of aquaculture-based restoration efforts for federally listed endangered corals will be accomplished through these indicators of success.

Progress 07/28/14 to 06/30/16

Outputs
Target Audience:The target audience is conservation biologists and those involved with the restoration of endangered corals- both in the United States and internationally. As part of our partnership with The Florida Aquarium, we will have the opportunity to display this system to the general public (those visiting the Center for Conservation) and use it as a vehicle to discuss the plight of corals in Florida and showcase the efforts of University of Florida research scientists to restore coral populations. Changes/Problems:The proposed water source, treated saline groundwater, was not available. High-quality sterilized natural seawater was trucked in from The Florida Aquarium in downtown Tampa 800 gallons at a time. This made efficient water usage an even higher priority. This demand was met by the very low water exchange rates made possible by biological conditioning in the refugium and protein skimmer. What opportunities for training and professional development has the project provided?A UF/IFAS Summer Research Internship was awarded to Timothy Lyons, a Marine Sciences major at UF, specifically to work with the new recirculating system. Tim was paid a total of $3,360 from Florida Agricultural Experiment Station funds for six weeks of coral aquaculture work and research experience. Following a recommendation by Dr. Joshua Patterson, his internship supervisor, Tim was subsequently awarded a highly competitive $4,000 academic scholarship from the Marine Aquarium Society of North America (MASNA). The committee awarding the MASNA scholarship stated that Tim's coral aquaculture internship with UF/IFAS was a primary reason for his selection. Tim Lyons showing off the recirculating system he helped build and operate (Left) and preparing experimental coral fragments for the system (Right). The aquaculture program at Hillsborough Community College has a degree requirement for students to successfully complete three aquaculture-related internships. Paperwork was completed and Christopher Kilroy has spent the past six months working with Dr. Joshua Patterson on the newly constructed experimental coral recirculating system in fulfillment of one of his required internships. This continues a tradition of collaboration between Hillsborough Community College and UF/IFAS Aquaculture, which to this point has existed primarily at the Tropical Aquaculture Laboratory in Ruskin. Chris works approximately 10 hours per week and has gained knowledge and experience in seawater chemistry management, coral biology and husbandry (photograph at left), and recirculating system engineering. Experience and skills gained and connections made working with the coral recirculating system will make Chris a more marketable job candidate post-degree. An undergraduate from the University of Tampa (Bridget Hickey - B.S. Marine Science-Biology; May 2015) was registered as a volunteer with the University of Florida and worked on the coral recirculating system project under the supervision of Dr. Joshua Patterson. Finally, a distance education student residing in neighboring Pinellas County and beginning the Master's in Fisheries and Aquatic Sciences program in Spring 2016 (Samantha Schaberg - B.S. Biology; Principia College; December 2013) also works one day per week with the coral recirculating system. How have the results been disseminated to communities of interest?Discussions have been initiated with scientists at other institutions (Smithsonian Marine Station at Ft. Pierce, Mote Tropical Marine Laboratory, and Nova Southeastern University) who are also growing corals in ex-situ systems. Results of this project will be shared specifically within that community of interest. When the Center for Conservation is opened to the public, members of the community and other interested parties will be invited to observe and discuss the research taking place. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? A fully operational research-scale coral aquaculture recirculating system has been constructed. The approximately 600 gallon system includes six 50-gallon acrylic tanks, a 150 gallon refugium, a 150 gallon sump, a protein skimmer, six stands, a magnetic drive pump, and six propeller driven pumps with three control boxes. This design allows for two sets of three replicate tanks which are currently being used to investigate the effects of differing flow regimes on corals grown ex-situ. Our focal coral species, staghorn coral Acropora cervicornis, is ESA listed as threatened. We have the good fortune of a terrific working relationship with the Coral Restoration Foundation (CRF), a pioneering not-for-profit that operates a series of in-situ nurseries throughout the Florida Keys. Not only were we able to source our corals from CRF for only the cost of shipping, the scientific nature of our work allows us to fall within permits for possession currently held by CRF. Including a back-up magnetic drive pump purchased with University of Florida funds and additional tanks and components donated by The Florida Aquarium, the $3,000 Giglia Endowment Award for Innovative Agriculture was leveraged to construct a system with a total value of over $8,000. Beyond monetary funding, this project has strengthened a partnership between UF/IFAS, The Florida Aquarium, and CRF, which was a stated goal of the proposal.

Publications


    Progress 10/01/14 to 09/30/15

    Outputs
    Target Audience:The target audience is conservation biologists and those involved with the restoration of endangered corals- both in the United States and internationally. As part of our partnership with The Florida Aquarium, we will have the opportunity to display this system to the general public (those visiting the Center for Conservation) and use it as a vehicle to discuss the plight of corals in Florida and showcase the efforts of University of Florida research scientists to restore coral populations. Changes/Problems:The proposed water source, treated saline groundwater, is not available. High-quality sterilized natural seawater is trucked in from The Florida Aquarium in downtown Tampa 800 gallons at a time. This has made efficient water usage an even higher priority. This demand is met by the very low water exchange rates made possible by biological conditioning in the refugium and protein skimmer. What opportunities for training and professional development has the project provided? A UF/IFAS Summer Research Internship was awarded to Timothy Lyons, a Marine Sciences major at UF, specifically to work with the new recirculating system. Tim was paid a total of $3,360 from Florida Agricultural Experiment Station funds for six weeks of coral aquaculture work and research experience. Following a recommendation by Dr. Joshua Patterson, his internship supervisor, Tim was subsequently awarded a highly competitive $4,000 academic scholarship from the Marine Aquarium Society of North America (MASNA). The committee awarding the MASNA scholarship stated that Tim's coral aquaculture internship with UF/IFAS was a primary reason for his selection. Tim Lyons showing off the recirculating system he helped build and operate (Left) and preparing experimental coral fragments for the system (Right). The aquaculture program at Hillsborough Community College has a degree requirement for students to successfully complete three aquaculture-related internships. Paperwork was completed and Christopher Kilroy has spent the past six months working with Dr. Joshua Patterson on the newly constructed experimental coral recirculating system in fulfillment of one of his required internships. This continues a tradition of collaboration between Hillsborough Community College and UF/IFAS Aquaculture, which to this point has existed primarily at the Tropical Aquaculture Laboratory in Ruskin. Chris works approximately 10 hours per week and has gained knowledge and experience in seawater chemistry management, coral biology and husbandry (photograph at left), and recirculating system engineering. Experience and skills gained and connections made working with the coral recirculating system will make Chris a more marketable job candidate post-degree. How have the results been disseminated to communities of interest?Discussions have been initiated with scientists at other institutions (Smithsonian Marine Station at Ft. Pierce, Mote Tropical Marine Laboratory, and Nova Southeastern University) who are also growing corals in ex-situ systems. Results of this project will be shared specifically within that community of interest. When the Center for Conservation is opened to the public, members of the community and other interested parties will be invited to observe and discuss the research taking place. What do you plan to do during the next reporting period to accomplish the goals?Finalise the above

    Impacts
    What was accomplished under these goals? A fully operational research-scale coral aquaculture recirculating system has been constructed. The approximately 600 gallon system includes six 50-gallon acrylic tanks, a 150 gallon refugium, a 150 gallon sump, a protein skimmer, six stands, a magnetic drive pump, and six propeller driven pumps with three control boxes. This design allows for two sets of three replicate tanks which are currently being used to investigate the effects of differing flow regimes on corals grown ex-situ. Our focal coral species, staghorn coral Acropora cervicornis, is ESA listed as threatened. We have the good fortune of a terrific working relationship with the Coral Restoration Foundation (CRF), a pioneering not-for-profit that operates a series of in-situ nurseries throughout the Florida Keys. Not only were we able to source our corals from CRF for only the cost of shipping, the scientific nature of our work allows us to fall within permits for possession currently held by CRF. Including a back-up magnetic drive pump purchased with University of Florida funds and additional tanks and components donated by The Florida Aquarium, the $3,000 Giglia Endowment Award for Innovative Agriculture was leveraged to construct a system with a total value of over $8,000. Beyond monetary funding, this project has strengthened a partnership between UF/IFAS, The Florida Aquarium, and CRF, which was a stated goal of the proposal.

    Publications


      Progress 07/28/14 to 09/30/14

      Outputs
      Target Audience: The target audience is conservation biologists and those involved with the restoration of endangered corals- both in the United States and internationally. As part of our partnership with the Florida Aquarium, we will have the opportunity to display this system to the general public (those visiting the Center for Conservation) and use it as a vehicle to discuss the plight of corals in Florida and showcase the efforts of University of Florida research scientists to restore coral populations. Changes/Problems: The greenhouse at the Center for Conservation which was under construction at the time of project funding is not yet operational because it lacks electricity and other important components. A required treatment system for the saltwater well has not yet been installed, so this water will not be available for the recirculating system. Greenhouse completion should take place within the next month, and sterile seawater will be hauled from The Florida Aquarium for use in the recirculating system. What opportunities for training and professional development has the project provided? An undergraduate from the University of Tampa (Bridget Hickey – B.S.Marine Science-Biology expected 2015) has been registered as a volunteer with the University of Florida. Bridget has received training on construction of recirculating coral aquaculture systems related to this project. How have the results been disseminated to communities of interest? Discussions have been initiated with scientists at other institutions (Smithsonian Marine Station at Ft. Pierce, Mote Tropical Marine Laboratory, and Nova Southeastern University) who are also growing corals in ex-situ systems. Results of this project will be shared specifically within that community of interest. When the system and its associated facility are operational, members of the public and other interested parties will be invited to observe and discuss the research taking place. What do you plan to do during the next reporting period to accomplish the goals? We plan to complete construction of the recirculating system and acquire water, live-rock, live-sand, and corals. Soon thereafter the system will be totally functional and will be maintained with living Acropora cervicornis and utilized for replicated research to test the effects of flow regime on coral growth and health.

      Impacts
      What was accomplished under these goals? Components for a research-scale coral aquaculture recirculating system have been acquired. This includes six 50-gallon acrylic tanks, six stands, a magnetic drive pump, and six propeller driven pumps with three control boxes. These components will facilitate construction of a recirculating system with three replicate tanks serviced by currently utilized low-technology surge flow regimes and three replicate tanks with the ability to better replicate tidally-driven flows experienced by corals in nature. Design and construction of the system has begun in consultation with John Than, who serves as Manager of the Center for Conservation for the Florida Aquarium and has experience growing corals on commercial scale.

      Publications