Source: COLORADO STATE UNIVERSITY submitted to
INTEGRATED AND INNOVATIVE APPROACHES TO REDUCE THE RISK OF LARKSPUR (DELPHINIUM SPP.) POISONING IN LIVESTOCK ON COLORADO FOOTHILLS RANGELANDS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
1003271
Grant No.
(N/A)
Project No.
COL00642A
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Jul 24, 2014
Project End Date
Jun 30, 2017
Grant Year
(N/A)
Project Director
Meiman, P.
Recipient Organization
COLORADO STATE UNIVERSITY
(N/A)
FORT COLLINS,CO 80523
Performing Department
Forest and Rangeland Stewardship
Non Technical Summary
Larkspurs (Delphinium spp.) are often considered the most damaging poisonous plants on rangelands in the western United States, including Colorado. Death losses of domestic cattle to larkspur poisoning have remained surprisingly consistent throughout the past century (3-5%, sometimes 15%). The economic impact of these losses exceeds $20 million annually. Other impacts occur that have not been quantified. Management options are severely limited in areas where larkspur occurs. Land and livestock managers are forced to use pastures with larkspur at very specific times to minimize poisoning. This can lead to the inability to harvest a substantial amount of forage and reduced opportunities to implement grazing strategies needed to maintain or improve rangeland conditions in areas without larkspur.In order to prevent livestock poisoning, animal managers typically avoid having cattle in pastures with significant amounts of Geyer larkspur during the growing season, and it is assumed that if Geyer larkspur is present, it is toxic (or highly toxic). As a result, pastures with Geyer larkspur may be used only in the winter resulting is severe limitations in the timing of use for these pastures and fewer options for grazing management. However, Geyer larkspur toxicity may vary from year to year, so if livestock managers can identify low-toxicity years early in the growing season, they may be able to use pastures in those years that they would normally avoid for the entire growing season. One of the aims of this project is to begin describing seasonal patterns of larkspur toxicity from year to year and to determine whether or not it is possible to identify low toxicity years early in the growing season.Increasing forb diversity is expected to produce three main outcomes: reduced larkspur abundance through competitive interactions among plants; increased dietary variety maximizing the availability of nutrients and plant secondary compounds for detoxification of diterpenoid alkaloids in larkspur; and altered production of diterpenoid alkaloids in larkspur. Increasing plant diversity by seeding forbs is far more sustainable and socially acceptable than repeated herbicide application - the only other practice used to reduce larkspur abundance. This approach provides a number of additional benefits for livestock production, habitat improvement and ecosystem services not realized through herbicide application. The economic viability of seeding forbs should be greater than herbicide application in the long-run because the need for ongoing herbicide applications would be eliminated. However, seed costs of native forbs are extremely variable and may be quite high. Therefore, if this approach is developed further, future studies to determine the best forb seed mixtures based on ecologic and economic factors will be important. If this study produces promising results, future efforts should also address the effects of increased forb diversity on larkspur consumption and detoxification by cattle. Another aim of this project is to test two different forb seed mixtures for their ability to compete with foothills larkspur.
Animal Health Component
0%
Research Effort Categories
Basic
25%
Applied
75%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1210799107050%
3140799107050%
Goals / Objectives
The proposed project addresses an important problem in Colorado with very significant ties to the economic viability, economic sustainability and social acceptability of agricultural and natural resource management activities on Colorado rangelands. We expect to improve the ability of managers to predict years or seasons of reduced Geyer larkspur toxicity thereby greatly improving opportunities for livestock management in foothills rangelands where Geyer larkspur is abundant. We also expect that by increasing the diversity of desirable forbs on native rangelands in Colorado, it will be possible to significantly reduce low larkspur poisoning in cattle.Objectives of Project:Describe the seasonal toxicity of Geyer larkspur in the northern foothills of Colorado.Determine the extent to which early growing season Geyer larkspur toxicity and abundance predict toxicity and abundance in the remainder of the growing season.Identify forbs that are effective plant competitors with Geyer larkspur and therefore result in the greatest reduction in larkspur abundance.
Project Methods
The objectives of this study will be addressed using field studies conducted at the CSURF Maxwell Ranch in northern Colorado. For objectives 1 and 2, we plan to identify three sites in larkspur-infested rangelands to establish separate replicated plots for measurement of larkspur abundance (density and canopy cover) and collection of aboveground larkspur material for toxicity determinations. Objective 3 will be addressed using plots previously established at the Maxwell Ranch to evaluate seed mixtures of native and introduced forbs sown into sprayed and unsprayed Geyer larkspur. Seasonal patterns of Geyer larkspur toxicity will be determined by collecting aboveground larkspur material (leaves, stems and inflorescences) at 2 week intervals between early May and late August of 2 or 3 years. Leaves, stems and inflorescences present at each collection date will be kept separate. Collected material will be oven-dried at 60 degrees Celsius for 48 hours to a constant weight. Dried leaves will be ground through a 1-mm screen, collected, and sent to the ARS Poisonous Plants Research Lab in Logan Utah to be analyzed for toxic alkaloid concentration following the methods Gardner et al. (1997). Density and canopy cover of larkspur will also be quantified at the beginning of the growing season and when larkspur is in full bloom. Early growing season toxicity and abundance of Geyer larkspur will be compared to values derived later in the growing season to determine the extent to which early measures predict values obtained later in the growing season. Predictors of toxicity to be tested will include various combinations of collection date and plant part. Identification of forbs that compete most effectively with Geyer larkspur will be accomplished by evaluating plots previously seeded with native or introduced forbs. Three sets of plots were established, each containing three complete blocks comprised of one plot for each of nine treatments. Plots were 3 x 10-m in size and were randomly assigned to one of the nine treatments within each block. A 0.5-m buffer was left between neighboring plots to minimize unwanted treatment overlap. Treatments consisted of all possible combinations of seeding (a native forb seed mixture, an introduced forb seed mixture and unseeded), herbicide (2,4-D LV4, picloram, and unsprayed) and location (set 1, set 2 and set 3). The native forb seed mixture was comprised of 11 species and the introduced forb seed mixture included 10 species. 2,4-D LV4 was applied at a rate of 2 quarts of product per acre (2.96 kg a.i. ha-1) and picloram was applied at a rate of 1 pint of product per acre (0.28 kg a.i. ha-1). The herbicides were applied the third week of June 2011 when approximately half the larkspur plants had bolted. The seed mixtures were planted in the fall to allow cold stratification and maximize early moisture for successful seedling establishment. Seeds were planted using a Truax Flex-II 86 rangeland seed drill. Each seed mixture was seeded a rate of 1000 PLS per square meter. All plots were seeded in late November 2011. In 2014, 2015 and 2016, canopy cover by species for all species present, and density by species of seeded species and larkspur will be determined from 6, permanently located 0.5m2 rectangular quadrats in each plot. Analysis of variance will be used to determine the effects of seed mixture and herbicide treatment on seeded forb and larkspur abundance.

Progress 07/24/14 to 06/30/17

Outputs
Target Audience:Information from this project has been communicated to individuals at state and local natural resource professional meetings, at field days/tours and in classes. The target audience includes scientists, graduate and undergraduate students, natural resource specialists (agency), consultants (private), livestock producers and land managers. Undergraduate and graduate students enrolled in classes taught by the research team and those who worked on the project have received information about the project on multiple occasions. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project has provided the opportunity for graduate and undergraduate students to gain experience in proposal development, field data and sample collection, plant sample processing, and data entry. To date, we estimate that at least 12 undergraduate students have been engaged in the project, from field data collection to lab preparation. The PhD student on the project has greatly expanded the scope of the work, with an ambitious but realistic goal of changing the understanding and approach to grazing management in larkspur habitat. As part of his work, he is studying and integrating knowledge from diverse disciplines, from social-ecological systems research to agent-based modeling to plant physiology. How have the results been disseminated to communities of interest?The PI and graduate student on this project have made presentations at the CSU Extension Educator meetings as well as several presentations to other students. Investigators also presented at the annual meeting of the Society for Range Management (2017). Additionally, the project has begun to engage individual ranches as partners in the work, with one having hosted field research this past summer, and several expressing interest in engaging in the coming months. An article highlighting some key aspects of this project was recently published in The Fence Post: Breeder's Connection 2017. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? With the assistance of the USDA Poisonous Plants Laboratory, we are approaching a clear understanding of the seasonal toxicity patterns of Geyer Larkspur, and have confirmed that these patterns are similar to what is in the literature for many other larkspur species. Alkaloid concentrations are greatest early in the growing season (pre-bolt) and decline steadily throughout the growing season. This important finding indicates that it is likely that the results of our current and future work on management of grazing in larkspur habitat will apply to the many other species of larkspur that are problematic across the Western US. This work has led us to focus on the aboveground alkaloid pools as a more useful measure of poisoning risk. Alkaloid pool is the product of plant biomass and alkaloid concentration. We have also now established (for the first time for any larkspur species) that the biggest Geyer Larkspur plants contain the most alkaloid toxins. Work begun this year and targeted for completion in 2018 is also indicating that it is highly likely that larkspur plants that are grazed will become smaller and less toxic. These findings suggest that seasonal avoidance, the dominant approach to grazing management in larkspur habitat, may not only be ineffective (which seems to be the case) but in fact may be counterproductive by letting Geyer Larkspur plants complete their life cycle without getting grazed. Given our recent findings, we are no longer pursuing research on the relationship between competing species and larkspur toxicity and abundance and instead are focusing on a complexsystems-based interdisciplinary research program to identify a strategic framework for active grazing management in larkspur habitat.

Publications

  • Type: Journal Articles Status: Under Review Year Published: 2018 Citation: Jablonski, J.E. and P.J. Meiman. (in review) Derivation of a non-destructive predictive model for Geyers larkspur mass. Rangeland Ecology and Management


Progress 10/01/15 to 09/30/16

Outputs
Target Audience:Information from this project has been communicated to individuals at state and local natural resource professional meetings, at field days/tours and in classes. The target audience includes scientists, graduate and undergraduate students, natural resource specialists (agency), consultants (private), livestock producers and land managers. Undergraduate and graduate students enrolled in classes taught by the research team and those who worked on the project have received information about the project on multiple occasions. Some key aspects of this project were recently discussed in an agricultural production magazine (The Fence Post; Breeder's Connection 2017). Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project has provided the opportunity for graduate and undergraduate students to gain experience in proposal development, field data and sample collection, plant sample processing, and data entry. To date, we estimate that at least 12 undergraduate students have been engaged in the project, from field data collection to lab preparation. The PhD student on the project is now in his fourth semester and has greatly expanded the scope of the work, with an ambitious but realistic goal of changing the understanding and approach to grazing management in larkspur habitat. As part of his work, he is studying and integrating knowledge from diverse disciplines, from social-ecological systems research to agent-based modeling to plant physiology. Additionally, the main undergraduate assistant from this past summer is now pursuing greenhouse-based research on larkspur toxicity and has expressed interest in continuing larkspur research into graduate school. How have the results been disseminated to communities of interest?While actionable results are still somewhat lacking (though expected by project's end) the investigators have begun reaching out to communities of interest. This has included presentations at the CSU Extension Educator meetings as well as several presentations to other students, a presentation at the state Society for Range Management annual meeting and an upcoming technical presentation at the annual meeting of the Society for Range Management 2017. Additionally, the project has begun to engage individual ranches as partners in the work, with one having hosted field research this past summer, and several expressing interest in engaging in the coming months. A major priority for the work is that the results be practical and actionable for land managers, and we expect that we will reach this goal by the end of the project period. An article highlighting some key aspects of this project was recently published in The Fence Post: Breeder's Connection 2017. Presentations during the reporting period: November 11, 2015, Geyer Larkspur Research Update, CSU Extension Forum, Fort Collins, CO February 1, 2016, A Novel Approach to Managing Larkspur Toxicity, Society for Range Management, Corpus Christi, TX What do you plan to do during the next reporting period to accomplish the goals? With goals one and two largely accomplished and goal three having yielded interesting results but nothing actionable, the work is now shifting to a multi-faceted effort aimed at defining a new approach to grazing management in larkspur habitat by the spring of 2019. The PhD student on the project has recently successfully presented his research agenda to his committee, with the following goal and objectives: Goal: Identify a new strategic framework for the management of cattle grazing in Geyer larkspur habitat that guides management decision making such that cattle can be grazed at any time and regardless of plant toxicity while eliminating the risk of poisoning death. Objective 1: Define a complex systems-based conceptual framework for the research. Objective 2: Improve understanding of Geyer larkspur toxicity dynamics and potential response to grazing. Objective 3: Develop a spatially-explicit individual-based model to simulate cattle grazing in Geyer larkspur habitat in order to identify key factors that lead to poisoning and pinpoint management-relevant thresholds. Objective 4: Conduct a systems analysis of representative cases of grazing management in Geyer larkspur habitat to improve understanding of viable strategies. Objective 5: Integrate the results of objectives 1-4 to propose a practical strategic framework for grazing management in larkspur habitat. While the completion of these objectives will extend beyond the life of this grant, the current funding has established the foundation for the work, and demonstrated that significant progress on grazing management in larkspur habitat, one of the oldest and most intractable challenges in the Western US, may be achievable in the near future.

Impacts
What was accomplished under these goals? We now have seasonal toxicity data for Geyer Larkspur for 2013, 2015, and 2016. With the assistance of the USDA Poisonous Plants Laboratory, we are approaching a clear understanding of the seasonal toxicity patterns of Geyer Larkspur, and have confirmed that these patterns are similar to what is in the literature for many other larkspur species. This important finding indicates that it is likely that the results of our current and future work on management of grazing in larkspur habitat will apply to the many other species of larkspur that are problematic across the Western US. In addition to the work of previous years in establishing the in-season relationships between larkspur abundance and toxicity, we have also now established (for the first time for any larkspur species) that the biggest Geyer Larkspur plants contain the most alkaloid toxins. Work begun this year and targeted for completion in 2018 is also indicating that it is highly likely that larkspur plants that are grazed will become smaller and less toxic. These findings suggest that seasonal avoidance, the dominant approach to grazing management in larkspur habitat, may not only be ineffective (which seems to be the case) but in fact may be counterproductive by letting Geyer Larkspur plants complete their life cycle without getting grazed. Given our recent findings and future research objectives (discussed below), we are no longer pursuing research on the relationship between competing species and larkspur toxicity and abundance and instead are focusing on a complex-systems-based interdisciplinary research program to identify a strategic framework for active grazing management in larkspur habitat.

Publications


    Progress 10/01/14 to 09/30/15

    Outputs
    Target Audience:Information from this project has been communicated to individuals at state and local natural resource professional meetings, at field days/tours and in classes. The target audience includes scientists, graduate and undergraduate students, natural resource specialists (agency), consultants (private), livestock producers and land managers. Undergraduate and graduate students enrolled in classes taught by the research team and those who worked on the project have received information about the project on multiple occasions. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project has provided the opportunity for graduate and undergraduate students to gain experience in proposal development, field data and sample collection, plant sample processing, and data entry. To date, we estimate that at least 10 undergraduate students have been engaged in the project, from field data collection to lab preparation. Additionally, a new PhD student has joined the project and has been engaged in every aspect of project management. Last, the investigators on the project have presented information about the project (including preliminary results) in classes to reinforce the material taught in those courses. How have the results been disseminated to communities of interest?While actionable results are forthcoming and fully expected by project's end, the investigators have begun reaching out to communities of interest. This has included presentations at the CSU Extension Educator meetings as well as several presentations to other students and an upcoming technical presentation at the annual meeting of the Society for Range Management. A major priority for the work is that the results be practical and actionable for land managers, and we expect that we will reach this goal by the end of the project period. What do you plan to do during the next reporting period to accomplish the goals?During the next period we will continue to solidify our understanding of objectives one and two. However, initial results have suggested that the most robust larkspur plants are also the most toxic, and we will be altering some aspects of the work to explore this proposition. If true, this would imply that current management recommendations focused on avoidance are leading to more toxic Geyer Larkspur plants, and consequent higher chances of death upon ingestion by cattle. Using a mixed approach that will incorporate field experiments and work with active ranchers, we will spend the next year examining whether grazing management strategies that utilize higher stock density can lead to lower Geyer Larkpur abundance and toxicity. Upon completion, we expect to be able to combine experimental data and real-world observation to develop a new strategy for effectively managing pastures with Geyer Larkspur.

    Impacts
    What was accomplished under these goals? We now have seasonal toxicity data for Geyer Larkspur from 2013 and 2015, having collected and analyzed (with the assistance of the USDA Poisonous Plants Laboratory) samples in both years during three stages of development: pre-bolt, bolt, and flower. While we will continue to sample in 2016 and perhaps beyond to confirm, it appears that Geyer Larkspur follows a similar seasonal toxicity pattern to the "tall" larkspur species. This seasonal pattern is well-published and begins with high alkaloid concentrations early in the growing season which gradually decline throughout the remainder of the growing season. Although this pattern is well known and well published for the tall larkspurs, confirming its existence in Geyer Larkspur is new. It is also evident that early season plant toxicity is not an indicator of future toxicity, an interesting result that compels future study and holds promise to identify novel approaches for successful livestock grazing management in areas where Geyer Larkspur grows. Early season abundance does appear to predict late season abundance. Perhaps due to poor growing conditions in sites where Geyer Larkspur grows, we have not been able to identify seeded species that effectively out-compete Geyer Larkspur, or even effectively establish themselves. However, we did find that one native species, Helianthus pumilus, may be an effective competitor, though the management utility of this finding appears limited. This aspect of the study has not been for naught in that the large dataset collected has been very useful in inspiring additional questions for study. All told, we have made significant strides in understanding Geyer Larkspur toxicity patterns, and initial study findings are being used to explore new questions, which we feel have the potential to significantly alter our understanding and management of Geyer Larkspur and all species of toxic larkspur.

    Publications


      Progress 07/24/14 to 09/30/14

      Outputs
      Target Audience: We expect that information from this project will be communicated to individuals in two main groups. The first group includes the audiences at state and local professional meetings. This includes scientists, graduate and undergraduate students, natural resource specialists (agency), consultants (private), livestock producers and land managers. The second group includes undergraduate and graduate students enrolled in classes taught by the research team and those who worked on the project. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? This project has provided the opportunity for graduate and undergraduate students to gain experience in proposal development, field data and sample collection, plant sample processing, and data entry. In addition, the investigators on the project have presented information about the project (including preliminary results) in classes to reinforce the material taught in those courses. How have the results been disseminated to communities of interest? Preliminary results have been disseminated to students taking courses taught by the investigators. Other than that, it is too early in the project for dissemination of results. What do you plan to do during the next reporting period to accomplish the goals? In the next reporting period, we expect to have completed the analysis of toxicity data from the first year of larkspur sample collection. We will also collect the second year larkspur samples and prepare them for the same type of analysis. We expect to have analyzed the preliminary data on forbs seeded to compete with larkspur.

      Impacts
      What was accomplished under these goals? Larkspur samples for the first year of the project have been collected from the field study site. We collected larkspur samples at three different stages of development; pre-bolt, bolt and flower. Samples have been dried, ground, packaged and shipped to the USDA ARS Poisonous Plants Lab in Logan Utah where toxic alkaloid concentrations will be determined. Field plots that will be used to identify effective forb competitiors with Geyer Larkspur have been established and preliminary data have been collected (but not yet analyzed).

      Publications