Source: WASHINGTON STATE UNIVERSITY submitted to NRP
BREEDING SUPERIOR STRAWBERRY CULTIVARS FOR THE PACIFIC NORTHWEST
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1003152
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 3, 2014
Project End Date
Jun 30, 2019
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
WASHINGTON STATE UNIVERSITY
240 FRENCH ADMINISTRATION BLDG
PULLMAN,WA 99164-0001
Performing Department
Horticulture & Landscape Architecture
Non Technical Summary
The strawberry industry in the Pacific Northwest (PNW) is distinct from other regions of the country. New strawberry cultivars adapted to the PNW environment, disease conditions, and market uses are needed. There are no day-neutral strawberry cultivars that have been developed in the PNW, so growers of day-neutral strawberries have to grow cultivars adapted to other regions. The breeding program will use traditional breeding methods and begin to incorporate molecular tools to develop high yielding, high quality, pest resistant strawberry cultivars that are adapted to local conditions and will benefit commercial growers, home gardeners and consumers.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
100%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2021122108120%
2031122108150%
2121122108120%
5031122108110%
Knowledge Area
212 - Pathogens and Nematodes Affecting Plants; 203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants; 202 - Plant Genetic Resources; 503 - Quality Maintenance in Storing and Marketing Food Products;

Subject Of Investigation
1122 - Strawberry;

Field Of Science
1081 - Breeding;
Goals / Objectives
To develop new short-day strawberry cultivars having several of the following traits: Improved yields; easy harvest; large, firm fruit of high quality for processing and/or fresh uses and durable plants with root rot tolerance, virus tolerance and aphid resistance.To develop new day-neutral strawberry cultivars having several of the following traits: improved yields; easy harvest; consistent fruit production over season; large, firm fruit of high quality for fresh use; durable plants with root rot tolerance, virus tolerance and aphid resistance
Project Methods
Traditional plant breeding methods will be used, with crosses made each year, seeds produced from these crosses germinated, seedling plantings established and selections made among the seedlings one year after planting. After strawberry selections are made, they are planted in 8-plant plots and observed for yield and fruit quality at WSU Puyallup. The most promising selections will go through virus elimination treatment and be evaluated in replicated plots for yield, fruit size, and fruit firmness. Those that continue to appear promising will be propagated for grower trials.

Progress 07/03/14 to 06/30/19

Outputs
Target Audience:The target audiences are strawberry plant breeders and strawberry growers. Presentations were made atan Oregon Strawberry Commission meeting. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?The results of theday-neutral and Junebearing strawberry effortswere reported to the Oregon Strawberry Commission andat other meetings. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? No June-bearing strawberry crosses were made in 2018 but selections were made in the 2017 seedling field. The short day seedlngs selected in 2018 from the 2017 seedling field will be evaluated in 2020 and 2021. In the planting established in 2016, WSU 3170 had the greatest yield and large firm fruit. It will continue to be evaluated in new plantings. Several selections in the 2017 planting had large firm fruit. They will be evaluated in 2019. Pollinations, seedling evaluations, selection identification, and selection evaluations are conducted yearly to develop new cultvars of day-neutral strawberries adapted to the growing regions of the Pacific Northwest, comprising the western regions of Washington, Oregon and British Columbia. In 2018, 261 seedlings were evaluated from threefamilies and two selections were made based on aconsistant pattern of fruiting, preliminary yield, plant architecture, and fruitquality. Additionally, a small selection trial was evaluated. This trial contained two day neutral cultivars, 'Albion' and 'Aromas' and four WSU day-neutral selections. Yield and fruit size data was collected from the selection trials in 2018. No new crosses were made in 2018, as the programfocused on propagating and evaluating further the selections currently in the program. With limited funding for this initiative, the selections have been maintained in tissue culture and in the greenhouse, awaiting adequate funding and opportunity for a replicated selection trial. Crosses underway in 2019 are focused on day-neutral by Junebearer types with an emphasis on fresh market varieties, but also evaluating for processed market potential. Four hundred seedlings from crosses with at least one day-neutral parent were germinated inFebruary 2019. A collabooration with geneticists at the USDA-ARS National Clonal GermplasmRepository is allowing us to test strawberry seedlings for the remontancey marker, which is underway. Based on the results of the marker test, the remontant seedlings will be planted and evaluated as day-neutral freshtypes, while the non-remontant seedlings will be planted and evaluated as Junebearing types, either for fresh or processed end use.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Hoashi-Erhardt, W.K. 2019. Fresh Strawberry Trends, Cultivars, and Research. Horticulture Growers Short Course, Abbotsford, BC. 24 January 2019.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Moore, Patrick P. 2019. Strawberries and Raspberries. Horticultural Grower's Short Course, Abbotsford, BC, 24, January 2019.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Hoashi-Erhardt, W.K. 2018. Fresh Strawberries in Washington and Pacific NW. Washington Small Fruit Conference, Lynden, WA 29 November 2018
  • Type: Conference Papers and Presentations Status: Submitted Year Published: 2019 Citation: Li,Z., R.K. Gallardo, W. Hoashi-Erhardt, V. McCraken, Wasko DeVetter. 2019. Supporting Successful Transition to the Fresh Market: Research and Extension Needs of Pacific Northwest Strawberry Growers. HortTechnology (Submitted).


Progress 10/01/17 to 09/30/18

Outputs
Target Audience:The target audiences are strawberry plant breeders and strawberry growers. Changes/Problems:The Washington Strawberry Commission has been disbanded. That was a major source of funding for the strawberry breeding program. The breeding program is shifting from a primarily short day strawberry program, to primarily a day-neutral program. As evaluation of the current short day strawberry selections is completed, the program will shift to an exclusively day-neutral program. What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Performance of strawberry selections has been presented to other plant breeders and to growers. What do you plan to do during the next reporting period to accomplish the goals?Continue to evaluate promising strawberry selections and continue the day-neutral strawberry breeding efforts.

Impacts
What was accomplished under these goals? Short Day Strawberry Cultivar Development Selections. Thirteen strawberry selections were made among the strawberry seedlings planted in 2017. The parents most represented among the selections were 'Charm' as a parent of 7 selections, WSU 2728 parent of 4 selections and WSU 3135 a parent of 3 selections. These selections will be planted in a new planting in 2018 and harvested in 2019 and 2020. Evaluation of selections. In the planting established in 2016, WSU 3170 and WSU 2646 had the greatest yield and had good fruit quality. Several selections in the 2017 planting had large firm fruit, but yields were less than expected. There were problems with the irrigation system in 2018 and yields were reduced this year. Day-neutral Strawberry Cultivar Development Seedlings We evaluated 388 seedlings from 9 families, and made 6 selections based on emphasis on consistent pattern of fruiting, plant architecture, and fruit quality. Selections Previous evaluations of non-replicated plots have identified 3 WSU selections that appear promising and need further evaluations in replicated plots and at other regional sites: WSU 14-27, WSU 12.216-3, and WSU 13.209-1. These evaluations have been delayed so far due to lack of funding. We were successful at securing a grant for evaluating day-neutral selections through a Specialty Crop Block Grant beginning in 2017 and through 2020. In September 2017, a replicated selection trial was planted with several promising selections along with 'Albion' and 'Aromas' as fruit quality and yield standards, respectively. First-year results will be available in fall 2018. Two selection trials of selections from WSU and cooperating breeding programs are slated to occur in fall 2018 and fall 2019 as well. New Project Targeting Fresh Market Through the project, "A thriving fresh market strawberry industry through breeding horticultural systems, grower resources, and nursery expansion", we will collaborate with Lisa DeVetter, Karina Gallardo, the NW Berry Foundation, and regional growers and nurseries to promote fresh market strawberry production in the NW region. The four main areas are plant breeding research, evaluation of horticultural practices, grower outreach, and plant propagation research for nursery expansion. Traditional breeding methods and molecular markers have resulted in a number of promising perpetual-flowering advanced selections from WSU and collaborators in OR and BC. These advanced selections will be trialed with standard commercial cultivars to examine their potential to have long seasons, high yields, and outstanding fruit quality for regional growers. The research in horticultural practices will focus on biodegradable mulching films (BDMs), which show promise in reducing field work and disposal expenses compared to polyethylene mulch. This project proposes to evaluate the mulching efficacy of available BDMs in establishment, overwintering, and spring and summer production of perpetual-flowering fresh-market strawberries. Thirdly, growers will be reached through a survey of their knowledge gaps and marketing needs, and also through two grower workshops sharing information on best production practices, postharvest handling, and marketing techniques of fresh market strawberries. Finally, three propagation methods will be examined for their impact on commercial field performance of fresh strawberry, which will help expand the capacity of nurseries that support the fresh market strawberry industry.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Finn, C.E., B.C. Strik, B.M. Yorgey, T.A. Mackey, P.P. Moore, M. Dossett, J. Lee, R.R. Martin, K.L. Ivors, A.R. Jamieson. 2018. Marys Peak Strawberry. HortScience. 53:395-400.


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:The target audiences are Rosaceae genomics, genetics and breeding scientists, bioinformaticists, and other crop database curators and developers. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Training opportunities for undergraduate,graduate and postdoctoral researchers included attendance and presentation at several conferences and meetings. The platform of the RosBREED project was used to disseminate concepts, tools, and knowledge to a cohort of future plant breeders and allied scientists. Throughout the year, email exchanges, teleconferences, in-person instruction, and seminar and conference presentations provided numerous opportunities for these graduate students to gain vital knowledge, skills, and experiences in DNA-informed breeding for fruit crops. Presentations on new approaches with modern genetics tools were given at the annual RosBREED participant meeting ("Breeding usefulness of visualizing genetic structure of genomes all at once") and at the ASHS annual conference ("What you see is what you can improve: Breeding utility of genome-wide haplotype mosaics"). Invited seminars were given at Wageningen University in the Netherlands and at Henan Agricultural University in China. At WSU, the PI advised three full-time postdocs, one PhD student, served on the advisory committee for another, mentored an undergraduate intern in fruit breeding, and three undergraduate lab assistants were trained. Guest lectures in undergraduate and graduate courses were also used to engage university students. How have the results been disseminated to communities of interest?Presentation "Strawberry and Raspberry Cultivar Development at Washington State University" at Lower Mainland Horticulture Improvement Association Short Course, Abbotsford, BC January 26, 2017. Strawberry Field Day, North Willamette Research and Extension Center, Aurora, OR, June 7, 2017. Strawberry Field Day, WSU Puyallup Goss Farm, June 14, 2017. Strawberry plant breeding progress communicated through written and oral reports to the Washington Strawberry Commission and the Oregon Strawberry Commission. Presented breeding program progress to NCCC212 meeting at College Station, PA, Oct. 26, 2017 Success stories, pitfalls to avoid, techniques, new genomics discoveries, new DNA tests, new DNA information on breeding germplasm, and new approaches, information, and tools were described to breeders, graduate students, breeding staff, allied scientists, and industry stakeholders via: RosBREED newsletters and webpages; oral presentations and posters conferences, meetings,workshops; email exchanges; teleconferences; personal visits; written reports; and scientific publications. DNA test cards and their brochures were displayed on the RosBREED website. Peer-reviewed publications delivered the scientific advances, as reviews or specific studies on traits, germplasm, and breeding programs.Presentations were made to the tree fruit industry at the annual Cherry Institute meeting, annual research reviews for cherry (Nov 2016) and apple (Jan 2017). Two sweet cherry Breeding Program Advisory Committee meetings were chaired by Dr. Peace. DNA information on cherry breeding selections was provided to industry members on request. Outputs and activities updates were presented at quarterly web-based meetings of the USRosEXEC. A newsletter was sent out to the GDR users mailing list and components of the GDR project were presented on 14 occasions at two local, one national (ASPB) and four international meetings (International Peach Symposium,PAG, PAG Asia, South Korea Plant Breeding and Genetics Symposium)as well as two peer-reviewed publications in year 2. A GDR training workshop was held at PAG with over 60 participants, Tripal database developer meetings were held as well as a 1.5 day hackathon, support was provided to groups implementing Tripal databases and the Tripal website (tripal.info) kept current with new developments, and module releases. The team participated in: monthly AgBioData meetings; the AgBioData workshop at the Plant and Animal Genome Conference; monthly meta data ontology working group meetings; the plant database booth at PAG; and at the annual participants meeting of the SCRI RosBREED project. In year 3 of this SCRI project, GDR was accessed by 21,515 users from 153 countries, with 275,117 pages viewed over 56,338 visits. What do you plan to do during the next reporting period to accomplish the goals?Project 0038 Continue to use strawberry selections and cultivars as parents that are large, firm-fruited, productive with good flavor to develop seedling populations with that combine these traits. Short-day and day-neutral selections will be made from these seedling populations. Seedlings and selections that are in the field will be evaluated. Old Project 0659 1) Upgrade GDR to Tripal 32) enable connectivity between GDR and other Tripal tree databases so users can cross query other databases from GDR to further enable comparative genomic opportunities 3) complete v2 of the Tripal breeding information management system (BIMS) 4) continue promoting further use of the FieldBook App for more efficient collection of phenotype data by Rosaceae breeding programs and allied scientists 5) continue to add large scale genomic, genetic and breeding data asavailable 6) present GDR at relevant conferences and meetings; continue providing Tripal support;actively participating in GGB database effortsincluding release of a white paper on Agricultural Biological Database data, code and communication sharing opportunities.

Impacts
What was accomplished under these goals? Project 0038 Short Day Strawberry Cultivar Development Selections. Twenty-three strawberry selections were made among the strawberry seedlings planted in 2016. These selections will be planted in single plots in 2018 and harvested in 2019 and 2020. Evaluation of selections In the planting established in 2015, there was one selection that combined high yields, large fruit, firm fruit, and good flavor. In the planting established in 2016, there were five selections out of the twenty in the planting that combined high yield, large fruit, firm fruit and good flavor.None of the promising selections have been planted in replicated plots. Selections that performed well will be propagated for re-planting in replicated plots. Day-neutral strawberry Cultivar Development Seedlings Three hundred eighty-eight seedlings from 9 families were planted, and 6 selections were made based on an emphasis on consistent pattern of fruiting, plant architecture, and fruit quality. Selections Previous evaluations of non-replicated plots have identified 3 WSU selections that look promising and need further evaluations in replicated plots and at other regional sites: WSU 14-27, WSU 12.216-3, and WSU 13.209-1. In September 2017, a replicated selection trial was planted with several promising selections along with 'Albion' and 'Aromas' as fruit quality and yield standards, respectively. First-year results will be available in fall 2018. Two selection trials of selections from WSU and cooperating breeding programs are slated to occur in fall 2018 and fall 2019 as well. Old Project 0679 Objective 1 - Approaches: Genomics discoveries were translated to tools and knowledge for breeding application for numerous U.S. fruit breeding programs by a systematic DNA test development approach. New trait-predictive DNA tests were developed and adapted for specific breeding programs. A new approach was devised and demonstrated for helping breeders experience the genomes of their germplasm: "Haplotype mosaics".Objective2 - Enable: DNA-informed breeding for rosaceous crops is now conventional in the U.S. Dr. Peace gave keynote or invited presentations at international and domesticconferences to audiences of breeders, allied scientists, and trainees.Talks at the international venues in late 2016 had an instructive tone ("Learning as we go: DNA-informed apple breeding at Washington State University"; "From QTLs to routine DNA-informed breeding: prospects, advances, & needs ...and experiences in apple at Washington State University?") and culminated in review-style publications in early 2017: "DNA-informed breeding of rosaceous crops: Promises, progress, and prospects" an invited paper forHorticulture Research; "Advances in marker-assisted breeding of apples", an invited chapter in thebook "Achieving Sustainable Cultivation of Apples". The pre-breeding apple program at USDA West Virginia was supported with new DNA tests and resulting DNA information on breeding parents and families. Several other breeding programs that this project has directly supported in previous years have now been connected with commercial service providers and successfully enabled to conduct routine DNA-informed breeding.Objective3 - Collaborate: Synergies were enhanced through national and international research collaborations and committee service. In 2016, Dr. Peace became Chair of RosEXEC, the U.S. Rosaceae Genomics, Genetics, and Breeding Executive Committee,ex officio in 2017 as Past Chair. Key collaborations continued with Dr. Main and the Genome Database for Rosaceae,a presentation by Dr. Peace in the NRSP10 workshop atASHS, andwith Dr. Craig Hardner (University of Queensland/QAAFI) for integrating quantitative genetics with new DNA marker-based opportunities for tree fruit breeding. Objective4 - Implement: Over the last year, WSU's apple breeding program managed by Dr. Evans and the sweet cherry breeding program managed by interim breeder Dr. Peace continued to be the primary beneficiaries from this project. Routine DNA-informed breeding at multiple breeding stages was conducted. Industry-funded projects were completed and others initiated that targeted specific high priority research targets to pursue and implement for delivery of outcomes for tree fruit growers of the Pacific Northwest. Old Project 0659 (1) Curation and addition of large scale genomic data- (a) 3 genome assemblies and annotations added: sweet cherry (v1.0.a1), peach (v2.0.a1), apple (GDDH13 v1.1), with GDR analyzed functional annotation available to, browse, search and download (b) Developed RubusCyc and updated the PeachCyc, FragariaCyc and AppleCyc databases (c) Reference transcriptomes (v1.0) generated from 9.6 billion RNASeq reads for apple, cherry, peach, strawberry and Rubus (v2.0). RefTrans are annotated and available to browse, search and download, and mapped to the genome sequence where available (d) over 900,000 markers added (e) 10,496,544 million SNP genotype measurement added to chado (f) NCBI genes for Rosaceae aligned to respective genomes and made available on genome browsers. (2)Curation and addition of genetic data- (a) All QTL updated using the standardized Riosaceae Trait Ontology (b) added 57 genetic maps, 3912 markers, 293 QTL/MTL added from 25 publications (c) added Rosbreed genotype and phenotype data from several programs. (3) Web interface and analysis pipeline development- (a) implemented new Tripal BLAST+ module that provides direct hyperlinks fromBLAST results to the database (b) developed and implemented a SNP marker search tool (c) developed and implemented a search by trait tool (d) implemented access to the IASMA Pathway Inspector tool though GDR. This embedded tool allows researchers to identify differentially expressed genes and provides a topology-based analysis of enriched pathways. Pathway Inspector is equipped with ad-hoc interactive graphical interfaces simplifying the discovery of modulated pathways and the integration of the differentially expressed genes in the corresponding pathway topology (e) completed and released three Tripal extension modules for data loading, search and display for sequence, map, marker, QTL, genotype, phenotype, germplasm (f) Identified sequences for a new 9K cherry array (g) Continued development of the Tripal Breeding Information Management System, all Clemson peach breeding program data added, currently being tested by the breeder. (4) Outreach - (a) promoted use of the FieldBook App for collection of phenotype data in experiments (b) presented GDR through peer-reviewed publications, presentations, specific GDR training workshop at PAG, newsletters and brochures (c) implemented suggestions from survey in GDR (d) actively participated in agricultural biological database (AgBioData) meetings including organizing a face-to-face workshop in Salt Lake City with over 40 scientists participated in developing a whitepaper on agricultural databases best practices (e) actively participated in Tripal community developer meetings (f) provided support for Tripal adoption and module development including several online training sessions. GDR by the numbers: By the end of year 3 of this project, GDR contained the following data: 14 genomes for 10 species; 285,855 genes and 332,249 mRNAs; 3,278,826 markers; 224 genetic maps; 2,901 trait Loci; 392,473 genotypes; 845,467 phenotypes; 18,700 germplasm; 1,967 species, and 7,062 publications. Old project 0677 The Dhingra program (or Integrated Genomics and Biotechnology Program) established that plastid development in apples differs across cultivars. The PI and his graduate student were awarded one U.S. patent on discovering methods to reverse the 1-methylcyclopropene induced blockage of ripening in pears. The program produced 7 peer-reviewed publications, one book chapter, one article in trade magazine and the PI was invited to present his work at several meetings.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Lin, Q., Herndon, N., Grau, E., Ficklin. S.P., Staton, M., Jung, S., Main, D., Feltus, A. and Wegrzyn, J.L. (2017) A Novel Tripal Database Module and Workflow to Facilitate Variant Mapping and Detection in Non-Model Plant Species. Abstracts of the International Plant and Animal Genome Conference XXV; January 14-18, 2017; San Diego, USA
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Ficklin, S.P., Cheng, C.H., Watts, N., Chen, M., Wytko, W., Soto, B., Sanderson, L.A., Jung, S., Wang, K-H., Staton, M., Main D., Feltus, A. and Wegrzyn, J.L. (2017). Tripal Introduction and Core Development Efforts. Abstracts of the International Plant and Animal Genome Conference XXV; January 14-18, 2017; San Diego, USA
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Jung, S., Cheng, C-H., Lee, T., Ficklin, S.P., Yu, J., Humann, J.L. and Main, D. (2017) Mainlab Chado Loader, Chado Data Display and Chado Search for Sequence, Map, Marker, QTL, Genotype, Phenotype and Germplasm Data. Abstracts of the International Plant and Animal Genome Conference XXV; January 14-18, 2017; San Diego, USA
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Gasic, K., Jung, S., Lee, T., Cheng, C-H., Yu, J., Campbell, T., Evans, K.M., Peace, C. and Main, D. (2016). Use of FieldBook and BIMS for Rosaceae Breeding. RosBREED Participants Meeting; March 6-8, 2017; East Lansing, USA
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Jung, S., Cheng, C-H., Lee, T., Ficklin, S.P., Yu, J., Humann, J.L. and Main, D. (2017) Development of Database Resources and Tools for Crop Genomics, Genetics and Breeding Research. Abstracts of the International Plant and Animal Genome Asia Conference XXV; May 29-30, 2017; Seoul, South Korea
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Jung, S., Cheng, C-H., Lee, T., Ficklin, S.P., Yu, J., Humann, J.L. and Main, D. (2017) Development of Database Resources and Tools for Crop Genomics, Genetics and Breeding Research. Abstracts of the 2017 Plant and Breeding Symposium; June 3-4, 2017; Seoul, South Korea
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Gasic, K., Jung, S., Lee, T., Cheng, C-H., Zheng, P., Humann, J.L., Ru, S., Scott, K., Frank, M., Yu, J., McFerson, J., Evans, K.M., Peace, C.P., DeVetter, L., Coe, M., Kahn, M. and Main, D., (2017). Peach Resources in the Genome Database for Rosaceae. Proceedings of the IX International Peach Symposium; July 2-6, 2017; Bucharest, Romania
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Jung, S., Lee, T., Cheng, C-H., Zheng, P., Humann, J.L., Ru, S., Scott, K., Frank, M., Yu, J., Gasic, K., McFerson, J., Evans, K.M., Peace, C.P., DeVetter, L., Coe, M., Kahn, M and Main, D. (2017). Using GDR: An Overview. Abstracts of the International Plant and Animal Genome Conference XXV; January 14-18, 2017; San Diego, USA
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Jung, S., Lee, T., Cheng, C-H., Zheng, P., Humann, J.L., Ru, S., Scott, K., Frank, M., Yu, J., Gasic, K., McFerson, J., Evans, K.M., Peace, C.P., DeVetter, L., Coe, M., Kahn, M and Main, D. (2017). Newly Designed Genome Database for Rosaceae (GDR). Abstracts of the International Plant and Animal Genome Conference XXV; January 14-18, 2017; San Diego, USA
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Campbell, J.D., Campbell, E., Jung, S., Main, D., Poelchau, M., Walls, R. and Harper, L.S. (2017) AgBioData: A Consortium of Agricultural Biological Databases. Abstracts of the International Plant and Animal Genome Conference XXV; January 14-18, 2017; San Diego, USA
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Buble, K., Jung, S., Humann, J.L., Cheng, C-H., Lee, T., Ficklin, S.P., Yu, J. and Main, D. (2017) Development of TripalMap. Abstracts of the International Plant and Animal Genome Conference XXV; January 14-18, 2017; San Diego, USA
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Moore, Patrick P. Strawberry and Raspberry Cultivar Development at Washington State University 2017 Lower Mainland Horticulture Improvement Association Short Course, Abbotsford, BC.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Howard NP, van de Weg E, Bedford DS, Peace CP, Vanderzande S, Clark MD, The SL, Cai L, Luby JJ (2017). Elucidation of the Honeycrisp pedigree through haplotype analysis with a multi-family integrated SNP linkage map and a large apple (Malus�domestica) pedigree-connected SNP data set. Horticulture Research 4:17003.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Sandefur P, Frett T, Clark J, Gasic K, Peace C (2017). A DNA test for routine prediction in breeding of peach blush, Ppe-Rf-SSR. Molecular Breeding 37:11.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Iezzoni A, Peace C, Main D, Bassil N, Coe M, Finn C, Gasic K, Luby J, Hokanson S, McFerson J, Norelli J, Olmstead M, Whitaker V, Yue C (2017). RosBREED2: progress and future plans to enable DNA-informed breeding in the Rosaceae. Acta Horticulturae 1172:115-118.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Cai L, Voorrips RE, van de Weg E, Peace C, Iezzoni A (2017). Genetic structure of a QTL hotspot on chromosome 2 in sweet cherry indicates positive selection for favorable haplotypes. Molecular Breeding 37:85.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Peace C (2017). DNA-informed breeding of rosaceous crops: Promises, progress, and prospects. Horticulture Research 4:17006.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Oraguzie NC, Watkins CS, Chavoshi MS, Peace C (2017). Emergence of the Pacific Northwest sweet cherry breeding program. Acta Horticulturae 1161:73-77.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Evans K, Peace C (2017). Advances in marker-assisted breeding of apples. Ch 8 in Achieving Sustainable Cultivation of Apples (Ed. K. Evans), Burleigh Dodds Cambridge, UK. pp 165-194
  • Type: Other Status: Published Year Published: 2017 Citation: Peace C (2017). Experiencing the genotype with haplotype mosaics  an Events article. Community Breeders Page, RosBREED Quarterly Newsletter 7(3): 6-7.
  • Type: Other Status: Published Year Published: 2017 Citation: Peace C (2017). RosBREED by the Numbers. RosBREED Quarterly Newsletter 7(2):10.
  • Type: Other Status: Published Year Published: 2017 Citation: Vanderzande S, Edge-Garza, D, Peace C (2017). DNA test conversion: An Upstream Research Approaches article. Community Breeders Page, RosBREED Quarterly Newsletter 7(2):7-8.
  • Type: Other Status: Published Year Published: 2017 Citation: Peace C (2016). Assessing your programs opportunities and how DNA information can help  an Events article. Community Breeders Page, RosBREED Quarterly Newsletter 7(1):7
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Norelli J, Evans K, Peace C (2017). Fire blight resistance and fruit quality in new Washington cultivars - Continuing Report. Washington Tree Fruit Research Commission Apple Research Review.
  • Type: Other Status: Published Year Published: 2017 Citation: Evans K, Peace C (2017). Apple scion breeding - Continuing Report. Washington Tree Fruit Research Commission Apple Research Review
  • Type: Other Status: Published Year Published: 2016 Citation: Peace C, Oraguzie N, Sandefur P (2016). After RosBREED: Developing and deploying new sweet cherry DNA tests - Final Report. Washington Tree Fruit Research Commission NW Cherry Research Review.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2017 Citation: Gasic, K., Jung, S., Cheng, C.H., Lee, T., Zheng, P., Yu, J., Humann, J., Evans, K., Peace, C., DeVetter, L., Mcferson, J., Coe, M.I. and Main, D. (2017). Resources in the Genome Database for Rosaceae for Peach Research. Acta Horticulturae (in press).
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2017 Citation: Jung, S., Lee, T., Cheng, CH., Humann, J., Yu, J., Ficklin, S.P. and Main, D. (2017). Extension modules for storage, visualization and querying of genomic, genetic and breeding data in Tripal databases. Database (Oxford) (in press)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Main, D., Jung, S., Lee, T., Cheng, C-H., Zheng, P., Humann, J.L., Ru, S., Scott, K., Frank, M., Yu, J., Gasic, K., McFerson, J., Evans, K.M., Peace, C.P., DeVetter, L., Coe, M. and Kahn, M. (2017). GDR: New Data and New Functionality. Abstracts of the International Plant and Animal Genome Conference XXV; January 14-18, 2017; San Diego, USA


Progress 10/01/15 to 09/30/16

Outputs
Target Audience:Strawberry growers. Strawberry plant propagators, plant breeders, strawberry researchers. Changes/Problems:No significant changes anticipated. What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Presentation "Strawberry and Raspberry Cultivar Development at Washington State University" at Lower Mainland Horticulture Improvement Association Short Course, Abbotsford, BC Strawberry Field Day, WSU Puyallup Goss Farm, June 1, 2016 Strawberry Field Day, North Willamette Research and Extension Center, Aurora, OR, June 8, 2016 Strawberry plant breeding progress communicated through written and oral reports to the Washington Strawberry Commission and the Oregon Strawberry Commission. Presented breeding program progess to NCCC212 meeting at Virginia Beach, VI What do you plan to do during the next reporting period to accomplish the goals?Continue to use strawberry selections and cultivars as parents that are large, firm-fruited, productive with good flavor to develop seedling populations with that combine these traits. Short-day and day-neutral selections will be made from these seedling populations. Seedlings and selections that are in the field will be evaluated.

Impacts
What was accomplished under these goals? Short Day Strawberry Cultivar Development Selections. Thirty strawberry selections were made among the strawberry seedlings planted in 2015. These selections will be planted in single plots in 2017 and harvested in 2018 and 2019. WSU 3023 (an early fruiting selection) and Puget Crimson (a large, late season cultivar) were the parents most represented in selections. Evaluation of selections In the replicated planting established in 2014, 'Charm', 'Totem', 'Tillamook' and WSU 3004, and WSU 2757 were the only ones that had cumulative two-year yields over 20 t/a. Only 'Tillamook' and 'Puget Crimson' had average fruit weight over 15 g. In the non-replicated planting the selection that appeared the most promising was WSU 2980. In the 2014 planting, there were some selections that had not established well in 2015, but were harvested in 2016. In the planting established in 2015, none of the selections were planted in replicated plots. Several of the plots were very productive, with large fruit, however, many of the plots did not establish well and primary evaluation this year was on fruit quality. Selections that performed well will be propagated for re-planting in replicated plots. Day-neutral strawberry Cultivar Development Seedlings and new selections Two hundred eighty-six seedlings from 13 families were evaluated, and 10 selections were made, with an emphasis on a consistent pattern of fruiting, plant architecture, and fruit quality. There was quite a bit of leaf spot damage in this planting. Small seedling fields in the last two years was a result of poor germination, and prompted us to germinate seeds in vitro. This was time-consuming but allowed us to generate a somewhat higher percent of germination, leading to a 2016 planting of 388 seedlings. Selections A non-replicated selection trial with WSU selections, MSU selections, and standard cultivars was evaluated in the first half of September 2015. Evaluations of these selections and cultivars continued May through September 2016. Remontancy marker testing in seedlings and selections (Funded by RosBreed II, SCRI) In 2016, in collaboration with Nahla Bassil and Jason Zurn at the USDA National Clonal Germplasm Repository, 19 day-neutral selections were used as parents for the Bx215_128 remontancy marker. Genotypes with this marker have a high probability of having a repeat-flowering fruiting habit. Fifteen of the selections were found to have the marker, while four were without it. This information will help determine the series of crosses to make next year to make progress in cultivar development for day-neutrals. Additionally, we participated in marker testing by planting 90 seedlings in 7 families from day-neutral crosses and testing them for the remontancy marker in the Bassil lab. These seedlings will be assessed for flowering habit over the next year to assign a phenotype to them, thus adding to the value of the remontancy marker for our own breeding purposes and for other strawberry breeders.

Publications


    Progress 10/01/14 to 09/30/15

    Outputs
    Target Audience:Strawberry growers, Strawberry plant propagators, Plant breeders, Strawberry researchers Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Presentation "Small Fruit Breeding at WSU Puyallup" at Lower Mainland Horticulture Improvement Association Horticultural Growers' Short Course, Abbotsford, BC attended by growers, plant propagators and plant breeders. Field day at WSU Puyallup June 4, 2015 attended by growers, plant propagators and plant breeders Presentation "Small Fruit Breeding at WSU Puyallup" at WA Small Fruit Conference & Lynden Ag Show 2015 December 1, 2015 attended by growers, plant propagators and plant breeders Strawberry plant breeding progress was also communicated through written reports and presentations to the Washington Strawberry Commission and the Oregon Strawberry Commission What do you plan to do during the next reporting period to accomplish the goals?Continue to use strawberry selections and cultivars as parents that are large, firm-fruited, productive with good flavor to develop seedling populations with that combine these traits. Short-day and day-neutral selections will be made from these seedling populations. Seedlings and selections that are in the field will be evaluated.

    Impacts
    What was accomplished under these goals? In the replicated planting established in 2014, 'Charm', 'Totem', WSU 2999 and WSU 2757 had yields over 10 t/a (Table 1). Only 'Tillamook' and 'Puget Crimson' had average fruit weight over 15 g. Only WSU 2757, 'Charm', 'Rubicon' and WSU 2357 had firmness over 200 g. In the non-replicated planting selections that appear the most promising are WSU 2980, WSU 3010 and WSU 3099. In the planting established in 2013, there were a number of selections that combined large fruit with good firmness. In the planting established in 2013,'Charm' had the greatest yield, with large fruit (Table 2). WSU 3006 had good yield, but fruit quality was not acceptable. In the non-replicated plots, WSU 2971 had the greatest yield and among the largest and firmest fruit. Activities continue for development of day-neutral strawberry cultivars. 2014 Spring planting. We evaluated 758 seedlings from 15 crosses. There were 18 selections with emphasis on consistent pattern of fruiting, plant architecture, fruit quality. 2014 Fall planting. 202 seedlings from 8 families were planted in September 2014. No selections were made in 2015; these seedlings will be carried over a second year for evaluation. Crosses are being planned for winter 2015-2016 for a new fall planting of seedlings in 2016. Evaluation of day-neutral selections. We planted a non-replicated selection trial with WSU selections, MSU selections, and standard cultivars in the first half of September. Evaluations of these selections and cultivars will be made beginning in Spring 2016.

    Publications


      Progress 07/03/14 to 09/30/14

      Outputs
      Target Audience: Strawberry growers, Strawberry plant propagators, Plant breeders, Strawberry researchers. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Continue to use strawberry selections and cultivars as parents that are large, firm-fruited, productive with good flavor to develop seedling populations with that combine these traits. Short-day and day-neutral selections will be made from these seedling populations. Seedlings and selections that are in the field will be evaluated.

      Impacts
      What was accomplished under these goals? Forty-five strawberry selections were made among the 3,100 June-bearing strawberry seedlings planted in 2013 under the previous project. These selections have been propagated for planting in 2015. Activities continue for development of day-neutral strawberry cultivars with seedlings evaluated in the 2013 and 2014 spring plantings with 10 selections made in the 2013 planting and 13 preliminary selections in the 2014 spring plantings. A fall planting of day-neutral seedlings was established in September 2014.

      Publications

      • Type: Journal Articles Status: Published Year Published: 2014 Citation: Finn, C.E., B.C. Strik, B.M. Yorgey, T.A. Mackey, P.P. Moore, M. Dossett, C. Kempler, R.R. Martin, A.R. Jamieson, and G.J. Galletta. 2014. Sweet Sunrise. HortScience. 49:1088-1092.