Source: HARRY SAUL MINNOW FARM, INC. submitted to NRP
EVALUATION OF A SPLIT-POND PRODUCTION SYSTEM FOR BAITFISH CULTURE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1003076
Grant No.
2014-33610-21925
Cumulative Award Amt.
$100,000.00
Proposal No.
2014-00140
Multistate No.
(N/A)
Project Start Date
Jun 1, 2014
Project End Date
Jan 31, 2016
Grant Year
2014
Program Code
[8.7]- Aquaculture
Recipient Organization
HARRY SAUL MINNOW FARM, INC.
7689 HIGHWAY 70 E
DE VALLS BLUFF,AR 720419985
Performing Department
(N/A)
Non Technical Summary
Arkansas produces 80 percent of all the baitfish in the US and annually exports more than 6 billion baitfish nationwide. However, baitfish farmers have suffered from a productivity reduction due to serious fish losses resulting from interminable predation and extensive inventory control in traditional ponds. Recently, unprecedented bird predation and unknown factors, possibly due to climate change and extensive management, have diminished baitfish population in the ponds. Since the SPS has been proposed as an alternative pond system in the catfish industry, baitfish farmers have been interested in applying the SPS to baitfish culture. The SPS is divided into two sections by a levee and intensively confines fish in a relatively small area (20% fish-basin and 80% treatment-basin). It allows further intensification and many other benefits (intensive management, improved inventory control, and efficient anti-predation). Although the SPS has been developed and applied for catfish culture on a commercial scale, the system has not yet been applied to baitfish culture. Because a baitfish culture regime is considerably different than a catfish culture regime or the regimes of other large food fish, many different elements must be taken into consideration when designing and operating the SPS and the system must be carefully modified for successful application to baitfish. We are seeking to optimize SPS technology for baitfish culture. The objectives include: Development of a water circulator and baitfish confinement barriers and evaluation of their engineering performance; Fish growth and economic analyses. We have closely communicated with the University of Arkansas at Pine Bluff on a prototype system and prepared its commercialization. If successful, the application of SPS to batfish culture will enhance our production and business regimes using energy efficient technology and will enable other baitfish farmers to cope with production challenges and problems that they have faced for a long time. The results will also help other baifish producers improve the profitability and sustainability of their operations, thus ensuring their future survival.
Animal Health Component
50%
Research Effort Categories
Basic
0%
Applied
50%
Developmental
50%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
40237152020100%
Knowledge Area
402 - Engineering Systems and Equipment;

Subject Of Investigation
3715 - Baitfish;

Field Of Science
2020 - Engineering;
Goals / Objectives
The baitfish farmers in Arkansas have suffered from a productivity reduction due to serious fish losses resulting from interminable predation and extensive inventory control in traditional ponds. Recently, unprecedented bird predation and unknown factors, possibly due to climate change and extensive management, have diminished baitfish production in ponds throughout Arkansas. Since the split pond system (SPS) has been proposed as an alternative pond culture system in the catfish industry, baitfish farmers have been interested in applying SPS to baitfish culture. Adaptation of these technologies will allow further intensification and many other benefits (intensive management, improved inventory control, and efficient anti-predation). Thus, the primary goal of this study is to develop a new SPS optimized for baitfish culture and management/operation criteria for baitfish, specifically fathead minnows. In order to accomplish this goal, the following three objectives must be met: 1) Objective 1: Development and performance evaluation of a water circulator Using the performance model of the UAPB water circulator, we will determine the dimension of our water circulator and retrofit it for baitfish use. The actual performance of our water circulator will be evaluated in terms of water flow and energy consumption, based on operational parameters in field conditions. 2) Objective 2: Development of fish barriers to retain small baitfish in fish basin We will develop a new concept of fish barriers. Unlike catfish or commercial culture of other fish species, one of the major obstacles in the commercialization of the SPS for small fish is retaining fish within the fish basin while simultaneously allowing for an appropriate water flow rate through the fish barriers. Thus, this study will design and manufacture prototype rotary screen fish barriers and test those barriers in field conditions. We will also evaluate the system's performance based on operational parameters and will identify the best set of operational and design improvements for future commercial application. 3) Objective 3: Evaluation of fish growth and economic feasibility Most importantly, we will evaluate the system's performance in terms of fish production. During the course of production, we will measure fish growth, size variation, and water quality changes. Finally, we will conduct an economic analysis considering all biological and engineering aspects obtained from Objective 1 and Objective 2, and compare this new production method with traditional pond culture techniques.
Project Methods
We will renovate a 5-acre pond on our farm into a SPS, use an University of Arkansas at Pine Bluff (UAPB) type water circulator, and install two rotary screen fish barriers. The pond will consist of a fish basin (0.5 acre, 10% of total water area) and a waste treatment basin (4.5 acre). The two basins will be divided by an earthen levee across the longer side of the pond and the levee will be constructed using the excavated soil obtained from deepening the fish basin. Two culverts will connect the two basins and a water circulator will be used to move the water. Each culvert will also have a rotary screen fish barrier to prevent the fish fron escaping. The water circulator will be placed in the waste treatment side and the two fish barriers will be mounted in the fish basin.In order to develop and evaluate the water circulator for a baitfish split pond, we will follow the general features found in the UAPB water circulator and manufacture a retrofitted water circulator and use a 3-hp AC electric motor (three-phase and 460V) having a rotation speed of 1750 rpm to rotate the circulator. The motor will be connected to an enclosed gear box and it will directly reduce the shaft rotation speed to 4-5 rpm. A variable frequency drive will also be installed to allow for further control of the paddle wheel rotation speed. Because the rotation speed and submergence depth of the paddles are key operational parameters, we will primarily estimate the preformance of the water circulator using different conbinations of the parameters under commercial conditions. On a commercial farm, it may be difficult to adjust the water depth to allow for different submergence depths of the paddles for experimental purposes. Thus,we will conduct intensive data collection as natural changes of water depth occur with any ordinary management regime. The rotation speed of the circulator will be adjusted using a variable frequency drive. The test rotation speeds will be 1, 2, 3, 4, and 5 rpms. The water velocity will be measured in a culvert using a velocity meter to calculate the flow rate of the water that is moved by the circulator. In addition, the actual amperages consumed by the circulator will be measured using adigital ammeter to assess the system's power consumption based on the different rotation speeds. That data (rotation speeds, water depth, water flow rates, and power consumption) will be integrated and processed using nonlinear regression analysis. The data will be used to empirically determine the motor capacity and the pertinent rotation speed for the required water flow.We will also test a disc type rotary fish barrier. A prototype of the rotary screen barrier will be built and tested to prove the concept and to observe any of the pitfalls it might have for commercial application. We will fabricate two fish barriers (8-foot diameter) and the frame of the barriers will be made with galvanized metal or aluminum plates. The disc will be radially divided into eight sections and each of the sections will be constructed in such a way that the replaceable radial peices of the screens will be securely mounted into the circle frame. A slow turning AC gear motor (1/4-1/2 hp, single phase and 115 V, 5-10 rpm range) will be connected to the disc at the center shaft and it will be used to constantly rotate the fish screen barrier. A variable frequency drive will be installed in order to further control the rotation speed. On the surface of the screen, a spring-loaded metal brush will be installed to scrape and clean off debris captured on the screen. The test rotation speeds will be initially set at 2 rpm (at that point, we will have variable water flow rates resulting from changes in the water circulator). At the given rotation speed and water flow rates, we will measure the head difference before and after the disc and exaluate the extent of bio-fouling over time. Moreover, if a field situation is allowed, we will remove the screened radial pieces and quantify the debris captured on the screen based on the various water flow rate conbinations in relation to the given rotation speed of the disc. If the head difference exceeds an acceptable level due to high bio-fouling at 2 rpm, we would adjust the disc rotation speed and continue the procedure to determine the best set of operations for the commercial application. The relationshp between the disc rotation speed, the water flow rates, and the head differnce over time (bio-fouling) will be analyzed using a non-linear regression method.Once we install the split pond with the water circulator and fish barriers, 100,000-150,000 young fathead minnows/acre will be stocked in the pond (total 500,000-750,000 in the fish basin) in mid-September and the fish will be raised in this environment until mid-November. In order to evaluate the growth performance of the fathead minnows, we will conduct seining in November and measure the total weight, survival rate, and feed conversion along with the size variation. The fish basin will be netted during the winter months (November - March) to protect fish from bird predation and the fish will be restocked for overwintering. Early the following year, we will conduct seining again and measure the total weight, the survival rate, the feed conversion, and the size variation. At the same time, we will run two traditional 5 acre ponds as control ponds and will follow the same culture regime to compare the production efficiency of the split pond with that of traditional ponds. This information will provide us with practical measurements we can use to compare the productivity, inventory control, and anti-predation of the split pond against existing traditional ponds already on our facility. In addition, over the course of the growth study, we will routinely collect water and examine the water quality in the fish and waste treatment basins. We will use standard farm management principles and collected data to develop variable and fixed cost of production estimates for each crop of baitfish produced in the split pond. This will allow us to compare costs of raising fish in split-ponds to historical production of baitfish in traditional earthen ponds at our facility. Variable costs for each crop will include those for fingerlings, feed, electricity, chemicals, labor, equipment maintenance, and interest on operating costs, etc. Fixed costs will be based on the initial construction costs of the split-pond system. An enterprise budget covering these costs will be developed and compared.Progress of this study will be evaluated periodically and will continue throughout the entire period of the project. Evaluation of the study will be based on milestones delineated below and whether pertinent actions for each topic of objectives are performed in a timely manner.- Completion of dirt work and dividing levee construction: by July 15th- Completion of setting up culverts: by August 15th- Completion of manufacturing a paddlewheel circulator and two fish barriers: by August 15th- Completion of setting up a paddlewheel circulator and two fish barriers: until August 31st- Completion of a test-run of a split pond: bySeptember 10th- Fish stocking: by September 15th- Mid-harvest: November 15th- Final harvest: January 15th

Progress 06/01/14 to 01/31/16

Outputs
Target Audience:The target audience reached by this effort include commercial fish farmers in Arkansas and state game and fish agencies interested in split pond production systems. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?The field work component of the project was completed in December 2015 due to a number of delays which were previously mentioned. Hence, dissimination of information to interested parties has just recently started. A presentation was given on the project to farmers and university researchers at the 2016Arkansas Bait & Ornamental Fish Growers Association Workshop in Lonoke on February 11, 2016. The talk generated quite a bit of interest and questions from the audience. In addition, farmers and researchers from both Arkansas and Mississippi have visited our farm to examine the baitfish split pond that was constructed. These efforts will be continued in 2016 as we run additional pilot trials with the system prior to submission of a Phase II proposal. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? With the success of SPS in catfish industry, we have paid a great deal of attention to the SPS for baitfish culture due to many desirable benefits, such as intensification, intensive management, improved inventory control, desirable anti-predation attributes, and effective disease treatment. Thus, our primary goal in this project was to develop a new split pond system and management/operation criteria for baitfish, specifically fathead minnows and to evaluate economic feasibility. We developed a 9.4 acre pond split pond system with a UAPB type water circulator and two rotary screen fish barriers to retain small fish. The pond consisted of a fish basin (0.94 acre) and a waste treatment basin (8.46 acre). Two culverts connected the basins divided by an earthen levee. A water circulator was placed in the waste treatment side and the two fish barriers were mounted in the fish basin side. We conducted a fathead minnow study to compare growth performance between split and traditional ponds and also economic analyses. Juvenile fathead minnows were stocked in the split pond at a rate of 153,948 fish/acre on September 3, 2015. A traditional pond (9.4-acre) was stocked with fathead minnows at a rate of 148,000 fish/acre. Fish were harvested three times on October 16 (partial harvest), November 5 (partial harvest), and December 10, 2015 to estimate survival and productivity. Total fish number harvested during the period appeared to be higher in the split pond. This might be attributed to the higher initial stocking rate. Overall gross yield and survival rate were, however, higher in the traditional pond. Fathead minnows in the two production systems progressively grew in body length and individual weight. Overall, growth rate in the traditional pond was higher than that in the split pond so that fish in the traditional pond were larger than those in the split pond at the end of study. The condition factor was higher in the traditional pond than the split pond, but there was no significant differences between the two production systems. Variations in the body length, weight, and condition factor were initially greater in the traditional pond, but became smaller with the course of time, and were similar at the end of study. Complete enterprise budgets were developed for production of fathead minnows using standard budgeting principles and methods. Separate budgets were developed for: 1) traditional ponds; and 2) the newly-constructed split-pond system. In the economic analysis, the returns with a 10 acre split pond were sufficient to cover all variable costs but not all the fixed costs. The lower yield from the split pond was not sufficient to cover the additional annual fixed costs that resulted from conversion of two 5-acre ponds to a single, 10-acre split pond. Thus, in comparison to the traditional pond, net returns from the split pond were lower than those in the traditional ponds. This was primarily due to the lower yield of fathead minnows in the split ponds, combined with the increased annual fixed costs resulting from the additional investment. However, breakeven yields above total costs for the 10-acre split pond were 401 lb/ac. This is a level of yield that is commonly attained in minnow ponds. Given that the yields used in the economic analysis (302 lb/ac) were from a single observation, it would be worthwhile to run the split pond for another season to obtain yield estimates from more than one observation. If yields of at least 401 lb/ac could be achieved, then the split-pond system for fathead minnows would be economically feasible (profitable over the long-term, after accounting for all costs that include non-cash costs of depreciation and opportunity costs on the investment capital). The additional investment in the split-pond system would need to be accompanied approximately by an additional 50 lb/ac of fathead minnows to result in the same level of economic returns as in traditional minnow ponds. During this project, the water circulator and fish barriers were successfully operated with minimal daily management. The water circulator was properly designed, enabling to pump water at different flow rates with an appropriate range of power consumption. One of the most important outcomes of this project was to develop the rotary disc screen fish barrier. The fish barrier was successful in retaining fish without serious operational and/or biological problems (mechanical jams, breakages, bio-fouling, and/or damage to fish due to disc rotation), allowing for sufficient water flow. This implies that the use of this type of fish barrier could be expanded to fingerlings production of other species, bringing an impact to other aquaculture industries. Although the production study did not indicate better performance of split pond with fathead minnows compared to a traditional pond approach, it might be too early to conclude the feasibility of the split pond based on the result. The lower production in the split pond might be attributed to the crowding effect and/or some other factors. Moreover, this study was conducted in late fall and we did not provide fish with optimal growing conditions during the summer and early fall when zooplankton abundances are much higher. In the smaller fish basin, the crowded fish were not provided with enough natural food that is a major food source for fathead minnows. Albeit with the lower production rate in this short study with the split pond, anticipated advantages of the SPS should not be taken lightly. If properly used, the application of SPS to batfish culture could, to a large extent, enhance our production and business regimes. This is supported by the economic analyses. In the economic analyses, breakeven yields above total costs for the 10-acre split pond were 401 lb/ac. This is a common level of yield easily attainable in fathead minnow ponds. Moreover, there are some potential advantages of split-pond systems that were not accounted for in this preliminary, proof-of-concept trial. Confining minnows to a smaller portion of a pond system would allow for better protection from losses due to bird depredation that have been shown to cause substantial economic losses on minnow farms. The survival of fathead minnows in split ponds also indicates that this system that includes a smaller fish containment section could be used feasibly to hold "weigh-back" fish that are returned following harvesting and attempted delivery without mixing with other sizes of fish. Thus, information and data derived from this study could enable Arkansas's baitfish farmers to cope with the problems that they have faced for a long time. In addition to helping baitfish farmers, the results of this study will also help other fingerling producers improve the profitability and sustainability of their operations, thus ensuring their future survival.

Publications


    Progress 06/01/14 to 05/31/15

    Outputs
    Target Audience: Nothing Reported Changes/Problems: There have been no majors changes in approach for this project. The only change is that we filed a no-cost extension to complete the project. Due to circumstances beyond our control, we have a late start on the project. We did not receive the funding to start the project until the first of October of 2014. The production season for raising fathead minnows is late spring through fall and we were beyond that window prior to receiving the grant funds. Prior to receiving funding in the fall we did go ahead and accomplish the dirt work to modify an existing 5 acre pond into a split pond system. However, we are still in the process of building and fabricating components of the split pond system that will be installed in the modified pond and used to conduct the study. Fabrication and installation of components of the split pond system will be completed by April of 2015 (Objective 1 and 2). The plan is to stock fish in the split pond system in April of 2015 and harvest in November of 2015 (Objective 3). Thus, we are requesting a no-cost extension be granted until January 31 of 2016 to provide sufficient time to analyze the collected data, conduct an economic analysis (Objective 3) and prepare our report. The engineering design work for Objective 1 (and nearly completed for objective 2) has been completed by the subcontractors at the University of Arkansas at Pine Bluff and we are using this information in the fabrication and installation process. What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? 2) Objective 2: Development of fish barriers to retain small baitfish in fish basin We are in the process of meeting the goals of Objective 2. Prior to fabricating rotary screen fish barriers (RoSFiB), we collaborated with UAPB personnel to develop an engineering design of fish barriers based on operational parameters such as rotational speed and required surface area of rotary screen. Considering the maximum pond water depth (1.22 m or 4 ft), the diameter of prototype RoSFiB were initially set at 8 ft in order to maximize the immersed surface area of screen. With the precondition, the effective submerged area, rotational speed and diameter of the rotary screen fish barrier were determined using an engineering equation developed by Boucher (1947) as follows.\ Equation 4 Also, rotational speed of RoSFiB can be estimated with the Equation 4 considering the effective submerged area, submerged surface area, and speed of strainer. Equation 5 Based on the rotational speed, torque (N-m) applied for motor and shaft can be derived by the following equation. Consequently required horse power of a motor and diameter of shaft can be estimated using torque. Each engineering parameter is summarized in Table 6. As the parameters are not well defined for our application (not for filtering uses), those were derived to our best knowledge and estimations considering similar applications. Equation 6 Pfb (motor power, hp) = (RPM T)/7161.75 Equation 7 Table 6. Values of engineering parameters applied for rotary screen fish barriers. Parameter Value Remark Precondition of rotary screen feature - Diameter (D, m) 2.43 (8 ft) - Submergence (%) 40 - Submerged surface area (Sa, m2) 2.26 (26.4 ft2) - Screen mesh size (mm) 3.18 (1/8 in) - Screen void ratio (%) 50 Defining engineering parameters for approximation of effective submerged area and rotational speed of rotary screen 1) m (constant) 0.0267 The values applied were referred by Ali (2013), best knowledge, and observations. 2) Q (water flow, m3/min) 25.7 (6,807 gpm) 3) Cf (initial resistance of clean filter mesh, m) 0.017 4) n (constant) 0.1337 5) I (filterability index, m-3) 0.05 6) s (Speed of strainer, m2/min) 2.4 7) Coefficient of discharge (unitless, clean screen) 0.6 8) Hscreen (friction head loss by screen, m) 0.2 Defining engineering parameters for approximation of effective submerged area and rotational speed of rotary screen 1) Expected weight of rotary screen (W, kg) 500 2) Inertia of a solid shaft (Is, kg-m2) 739 Is= W•(D/2)2 3) Angle acceleration (Aa, rad/sec2) 0.40 Aa= D•(RPM/60) 4) Maximum yield stress of shaft (Mpa) 3,600 Regular A36 steel shaft 5) Maximum yield stress of shaft (N/m2) 248,211,262 6) Safety factor (fraction) 4 Outdoor and continuous application 8) Allowable shear stress (N/m2) 62,052,816 Table 7. Results of estimating required rotational speed of rotary screen and sizing of a motor and shaft diameter Parameter Value Remark Approximation of effective surface and rotational speed of rotary screen - Required effective surface area (m2) 8.2 Equation 4 - Required rotational speed (rpm) 3.9 Equation 5, Adjustable rpm range: 0- 7.5 rpm - Torque (N-m) 1,195 Equation 6 Approximation of driving motor size and shaft diameter - Required minimum horse power of driving motor (hp) 0.16 at 3.9 rpm 0.61 at 7.5 rpm Equation 7.Using a variable frequency drive, rotational speed will be adjusted up to a maximum rpm of 7.5 in order to ensure enough water flow rate as needed. - Required minimum driving shaft diameter (Sdf, cm) 2.9 (1.14 in) at 3.9 rpm 3.6 (1.43 in) at 7.5 rpm Equation 3. To allow the motor and shaft to withstand force at the maximum rpm, we determined to install a 1 hp motor and a 1.43 cm (1-7/16 in) shaft. In order to understand the extent of bio-fouling to different mesh size of the screen for future application to smaller fish, UAPB personnel are modifying the standard filterability index apparatus proposed by Boucher (1947). The test apparatus will be made with 5 cm (2 in) transparent PVC pipes, and consists of a U-shaped flow mesh sizes of screen and hydraulic loading rates. The experimental mesh size of the channel, control valves, a flow meter, and a manometer. Pond water will be injected onto the side of one end of the U-shaped flow channel and pass through a screen mesh vertically mounted in the middle of the flow channel. As the water passes through the channel and the screen mesh, the changes of water head in the channel will be measured over time, based on the different screen will be 1/16 and 1/8 inches, and the experimental hydraulic loading rates will be set at 10, 20, and 30 m3/m2 min-1. The UAPB personnel are also currently fabricating fish barriers based on the engineering design in collaboration of our manufacturing team in real time. Up to date, fabrication is 70% complete and will be finished by the end of February, 2015. Objective 3 When we complete the fabrication of the paddlewheel water circulator/fish barriers and the construction of split pond by the mid-March, we will stock 100,000-150,000 young fathead minnows/acre in the pond (total 500,000-750,000 in the fish basin) in mid-April. The fish will be raised in this environment until early-November. In order to evaluate the growth performance of the fathead minnows, at the end experiment, we will measure total weight, survival rate, and feed conversion along with size variation. The fish basin will be netted during the experiment to protect fish from bird predation and survival rate will be compared to control ponds. This information will provide us with practical measurements that we can use to compare the productivity, inventory control, and anti-predation capability of the split pond against traditional ponds. In addition, over the course of the growth study, we will routinely collect water and examine the water quality in the fish and waste treatment basins. Dr. Roy will assist us with the overall data collection throughout the production period and Dr. Nathan Stone will consult with general management of fathead minnow culture in the SPS. We will use standard farm management principles and collected data to develop variable and fixed cost of production estimates for each crop of baitfish produced in the SPS. This will allow us to compare costs of raising fish in split ponds to historical production of baitfish in traditional earthen ponds at our facility. Variable costs for each crop will include those for fingerlings, feed, electricity, chemicals, labor, equipment maintenance, and interest on operating costs, etc. Fixed costs will be based on the initial construction costs of the split pond system. An enterprise budget covering these costs will be developed and compared. All economic analyses will be conducted by Dr. Roy and Dr. Carole Engle (Professor of Aquaculture Economics/Director at UAPB). Data to be used in the analysis will be collected by Dr. Roy and farm personnel. This analysis will be invaluable for development of the commercialization plan and expansion required during Phase II.

    Impacts
    What was accomplished under these goals? 1) Objective 1: Development and performance evaluation of a water circulator Considering biological and engineering preconditions for the application of the UAPB water circulator to baitfish culture, we collaborated with UAPB personnel to configure the setup of a split pond and develop an engineering design for a water circulator optimized for baitfish culture. The cultural and biological preconditions applied for this project are listed in Table 1 and 2. Table 1. Precondition of experimental pond configuration and culture management Pond Feature Culture management Pond size (ha) 2.02 (5 acre) Stocking density (fish/ha) 372,000 (150,000/ha) Fish basin ratio (%) 10 Total number of fish stocked 750,000 Water depth (m) 1.22 (4 ft) Harvest size (fish/kg) 318 (143/lb) Fish basin area (acre) 0.202 (0.5 acre) Survival rate 70 Fish basin water volume (m3) 2470.5 (652,643 gal.) Feeding ration (% of total body weight) / Protein content (%) 2 / 28 Table 2. Theoretical oxygen consumption and ammonia excretion rates Item Value Remark Daily oxygen consumption (DOC, g O2/day) 1,152 DOC = 36% of daily feeding rate (Tucker and Hargreaves 2004) Net protein utilization ratio (NPU) assumed 0.2 DAE (g/day) = (1 - NPU) x (protein fraction / 6.25) x 1000 x total daily feed amount (Tucker and Boyd 1985) Daily ammonia excretion rate (DAE, kg TAN/day) 1.14 With respect to the theoretical oxygen consumption and ammonia excretion rates, minimum required water flow rate was estimated using Mass Balance Analysis (Table 3). As a result, more water flow rate was required for ammonia removal than oxygen supplementation and the minimum water flow required for a baitfish split pond was approximated to be 25.96 m3/min (6,800 gallon/min) at the given preconditions. At the water flow rate, turnover time of split pond was estimated at 95 minutes. Table 3. Estimation of minimum required water flow rates based on DO consumption and TAN excretion rates Item Value Remark Target water quality requirements . - DO in inflow water (DOin, mg/L) 10 Based on water quality dynamics in commercial catfish split ponds (2 farms, 90 observations) measured for the past three years, DO and TAN requirements in inflow and outflow water has been established. TAN requirements were established considering safe levels for fathead minnows recommended by Russo and Thurston (1991) and theoretical no-observed-effect- concentration (NOEC, 9% of 96-h LC50, Ashe et al. 1996). - DO in outflow water (DOout, mg/L) 9 - TAN in inflow water (TANin, mg/L) 0.194 (0.065 as NH3 at pH 9 and 80 F) - TAN in outflow water (TANout, mg/L) 0.200 (0.048 as NH3 at pH9 and 80 F) Unit oxygen production and ammonia removal rates in split ponds - Unit oxygen production rate (UOP, g O2/kg feed•day-1) 32.35 The values are averages derived from the data collected in commercial catfish split ponds for the past three years (personal communication with Mathew Recsetar, Extension specialist at UAPB) - Unit ammonia removal rate (UAR, g TAN/kg feed•day-1) 33.93 Estimation of minimum required water flow rates (Q) - DO consumption basis (m3/min) 6.10 (1,614 gpm) Q = [(Daily feed amount x 0.36)-{(UOP x Daily feed amount)/1000}] / [1440 x {(DOin - DOout)/1,000,000}], (Losordo and Westers 1994) - TAN excretion basis (m3/min) 25.96 (6,869 gpm) Q = [{DAE - (UAR x Daily feed amount)/1000}] / [1440 x {(TANout - TANout)/1,000,000}], (Losordo and Westers 1994) The drag force equation was used to size a paddlewheel water circulator and a motor with respect to the rotational speed and wetted surface area of paddle, in order to pump the minimum required water flow rate estimated above. Generalized equations derived from the use of drag force equation for water flow rate, motor power, and driving shaft of paddlewheel circulator are as follows. Q (water flow rate, m3/min) = Σ [(0.5•Cd•ρw•cosθ•vb•Ab)/(1-k)]/1000 Equation 1 Ppw (motor power, HP) = Σ [0.0007•Cd•cosθ•vb3•Ab• (1-k)]/( Me•Ge) Equation 2 Equation 3 Engineering factors applied to approximate the minimum water flow rate and the motor capacity are presented in Table 4. Table 4. Engineering factors applied to size a paddlewheel water circulator Engineering factor considered Value or equation Drag Coefficient (Cd, unitless) 1.32 (Rouse 1946) Paddle angle (θ, degree) 45 Slip factor (k, fraction) 0.074+0.007•[ vb* / (3.14•wheel diameter)] *vb is tip velocity of paddle (m/sec), (Hendrick 2005) Density of Water (kg/m3) 998 Voltage (V) /wire phase 220 / 3 Motor energy transfer efficiency (Me, %) 85 Gear box energy transfer efficiency (Ge, %) 40 Using the mix and match approach between rotational speed and wetted area of paddle, we derived the sizes of paddlewheel and motor and presented the result in the Table 5. The maximum water depth of baitfish pond usually does not exceed 1.22 m (4 ft). Thus, allowable blade length that can be immersed was found to be 0.762 m (30 in), considering the existing paddlewheel circulators. As Park et al. (2014) recommended that the rotational speed of paddlewheel type circulators should not exceed 4-5 at a commercial catfish farm setting, we have set the standard rotational speed of paddle at 5 rpm. As a result, the width of blade was estimated to be 1.02 m (40 in) and water flow rate was expected to be 25.7 m3/min (6,807 gpm) accordingly. To pump the water flow rate, a 0.63 hp motor and a 4.32 cm (1.7 in) shaft were required. However, in order to ensure capability to pump enough water, we determined to use a variable frequency drive and install a motor (3 hp) and a shaft (6.19 cm or 2-7/16 in) that can run at a maximum rotational paddle speed of 7.5. Table 5. Results of sizing paddles, a driving motor, and a shaft Item Value Remark Target operational parameter - Maximum water depth (m) 1.22 (48 in) Considering support structures Adjustable rpm range : 0- 7.5 rpm - Allowable blade length (L, m) 0.762 (30 in) - Expected paddlewheel diameter (D, m) 2.59 (102 in) - Rotational speed (rpm) 5 Approximation of blade width and water flow rate - Paddle width (m) 1.02 (40 in) Mix and Match approach with combinations between the width and rotational speed of paddle using the drag force formula to achieve the minimum required water flow rate in Table 3. Equation 1. - Expected water flow rate at the given conditions (m3/min) 25.7 (6,807gpm) at 5 rpm 37.1 (9,813 gpm) at 7.5 rpm Approximation of driving motor size and shaft diameter - Required minimum horse power of driving motor (hp) 0.63 at 5 rpm 2.02 at 7.5 rpm Equation 2. Using a variable frequency drive, rotational speed will be adjusted up to a maximum rpm of 7.5in order to ensure enough water flow rate as needed. - Required minimum driving shaft diameter (Sdp, cm) 4.32 (1.7 in) at 5 rpm 5.59 (2.2 in) at 7.5 rpm Equation 3. To allow the motor and shaft to withstand force at the maximum rpm, we determined to install a 3 hp motor and a 6.19 cm (2-7/16 in) shaft. Based on this engineering design, we completed the construction of the earthen levee dividing fish basin (10%) and algae lagoon (90%). Currently we are fabricating a paddlewheel water circulator and will complete it by the end of February.

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