Progress 05/04/14 to 09/30/18
Outputs
Target Audience:Our target audience includes farmers who are the main consumers of our products as well as scientists and researchers who involved in plant breeding and genetics Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?1- Hybrid Breeding Program admitted three graduate students whose projects related to the hybrid rice developement. These students have received professional training in laboratory, greenhouse, and field operations. 2- The program encouraged the hybrid program employee to attend at the professionalworkshop and scientific meeting. 3- The program presented its latest findings to the scientific communities during symposium or scientific meetings as well as to farmers during Rice Expo, Field day etc. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?1- Our major focus will be to develop new experimental hybrid lines, some of which will be evaluated in ARPT trial. 2- We will continue developing hybrid parental lines. 3- We will continue collaborating with researchers from the University of Arkansas, and scientists from other academic institutions. In addition, we will extend our network with farmers in Arkansas as well as agricultural companies e.g. Riceland. 4- We will present our latest findings in the scientific meeting as well as farmers in Arkansas during Rice Expo, field day etc. These results will be published in B.R. Wells or other academic journals. 5- We will evaluate a station located in Belle Glade, FL as a new location of winter nursery for developing male sterile lines.
Impacts
What was accomplished under these goals?
1- We constructed a pipeline for developing male sterile lines. This pipeline includesnumbers of populations from early to advanced generations resulting from UA male steriles and high yield cultivars with good milling and eating qualities. For example, our advanced TGMS male sterile lines that possess genes associated with desirable agronomic traits, showed a good phenotypic charctersitics in field condition while demonstrated high percent of sterility. 2- We developed several experimental hybrid lines resulting from our new male sterile parent and elite rice cultivars/ University of Arkansas advanced rice lines. The results showed that the experimental hybrid lines produced more yield compare to the conventional rice cultivars used as checks. 3- Since we integrated genes/QTLs associated with cooking quality into the male sterile genome, the experimental hybrid rice described above showed good milling and cooking qualities. 4- We constructed a pipeline for developing restore line for hybrid rice production. In this pipeline, number of restorer populations from earlyto advance generations. Furthermore, for fthe first time, we sent seeds from these restorer lines to the winter nursery two advancetwo generations in each year. 5- We continued collaborating with researchers from the University of Arkansas, Division of Agriculture, Rice Research and Extension Center, Stuttgart, AR., University of Arkansas Department of Crop, Soil, and Environmental Sciences, Fayetteville, AR, and Dale Bumpers National Rice Research Center, Stuttgart, AR., on several projects regarding hybrid rice production and field management.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Eizenga, G., M. Jia,.., E. Shakiba,&, J. Edwards. 2018. Validation of yield component traits identified by GWA mapping in a tropical Japonica x tropical japonica rice bi-parental mapping population. Plant Genome; Date posted: July 21, 2018. doi: 10.3835/plantgenome2018.04.0021
- Type:
Other
Status:
Awaiting Publication
Year Published:
2018
Citation:
Shakiba,E., X. Sha, V. Boyett, D. North and K. Moldenhauer. Developing TGMS line for hybrid rice production in Arkansas, U.S.A. Proceeding of the 7th International Hybrid Rice Symposium, 27-28 February 2018, Yogyakarta, Indonesia. International Rice Research Institute. Los Ba�os (Philippines):
- Type:
Other
Status:
Published
Year Published:
2018
Citation:
Shakiba,E., K.A.K. Moldenhauer, X. Sha, P. Counce, D.G. North, D.E. Wood, V.A. Boyett, A. Rice, V.I. Thompson, and X. Ji. 2018. Developing hybrid parental lines. B.R. Wells Arkansas Rice Research Studies 2017; 651: 87-93
- Type:
Other
Status:
Published
Year Published:
2018
Citation:
North,D.G., K.A.K. Moldenhauer, P. Counce, E. Shakiba, and D.E. 2018. Identification of genetic sources of Arkansas male-sterile rice lines. B.R. Wells Arkansas Rice Research Studies 2017; 651: 63-70.
- Type:
Other
Status:
Published
Year Published:
2018
Citation:
Boyett, V.A., V.I. Thompson, E. Shakiba, X. Jin, and D.G. North. 2018. Advances in molecular analysis in a hybrid rice breeding program. B.R. Wells Arkansas Rice Research Studies 2017; 651:45-49
- Type:
Other
Status:
Published
Year Published:
2018
Citation:
Moldenhauer, K.A.K., X, Sha, E. Shakiba, D.K.A. Wisdom ETC. 2018. Breeding and evaluation for improved rice varieties the Arkansas rice breeding and development program. B.R. Wells Arkansas Rice Research Studies 2017; 651:57-62
- Type:
Other
Status:
Published
Year Published:
2018
Citation:
Sha, X., K.A.K. Moldenhauer, E. Shakiba, B.A. Beaty ETC. 2018. Development of superior medium-grain and long-grain rice varieties for Arkansas and the mid-south. B.R. Wells Arkansas Rice Research Studies 2017
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2018
Citation:
Rice, A., K.A, K. Moldenhauer, E. Shakiba, and A. Pereira. 2018. QTL mapping for yield traits in LaGrue rice. Rice Technical working group Conference, Feb.19-22, 2018. Long Beach, CL
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2018
Citation:
Shakiba,E., X. Sha, V. Boyett, D. North and K. Moldenhauer. Developing TGMS line for hybrid rice production in Arkansas, U.S.A. 7th International Hybrid Rice Symposium, 27-28 February 2018, Yogyakarta, Indonesia. International Rice Research Institute (IRRI).
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2018
Citation:
North, D.G., S. Fei, K.A.K. Moldenhauer, P. Counce, and E. Shakiba. 2018. Determination of genetic sources and threshold environment of Arkansas and GSOR male sterile lines. Rice Technical working group Conference, Feb. 19-22, 2018. Long Beach, CL
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2018
Citation:
Boyett, V.A, V.I. Thompson, X. Jin, X., and E. Shakiba. 2018. Improving efficiency of genetic analysis by Merging Capillary Electrophoresis and KASP methods. Rice Technical working group Conference.Feb. 19-22, 2018. Long Beach, CL
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Progress 10/01/16 to 09/30/17
Outputs
Target Audience:1- Along with other faculties, I hosted several delegations from Brazil, Russia, Serbia, Viet Nam, a visiting scientist from IRRI and a group of MSU graduate students. My role was to introduce Arkansas rice cultivars grown in exhibition bay as well as introducing hybrid rice program. I also showed RREC to some of these delegations. 2- Along with RREC faculties and DBNRRC-USDA-ARS scientist, I hosted students from the University of Arkansas, Pine Tree. As a representative of rice breeding program, I showed them RREC station and talked about rice breeding program 3- Hosting farmers at Rice College 2017. Dr. Sha and I met them at the exhibiting bay. My role was to talk about hybrid rice program and answer the farmer questions regarding rice breeding program. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals? Developing Environment Genic Male Sterile Lines (EGMS) EGMS is a genetic phenomenon that is used to develop two line F1 (hybrid) seeds in large scale. Previously, several EGMS lines were developed by the University of Arkansas Rice Research and Extension Center (RREC); however, these lines- despite high level of sterility- did not meet desirable agronomic traits for the hybrid rice production. We are developing several EGMS lines using 811s, 236s, and 805s and several high yield/good quality Arkansas rice cultivars. Currently, the status of developing these populations ranges from F1 to BC1F5 generation. In 2018, the early generation populations will be tested for A) % sterility through a greenhouse/field study, quality characteristics including amylose content, gelatinization temperature, and aroma; as well as disease resistance and plant height via molecular studies. The intermediate and advanced populations will be tested in field condition for agronomic characteristics such as % sterility, plant height, plant type, lodging, panicle exsertion, heading date, seed size, and panicle size. In addition, we will continue to initiate developing new male sterile lines by crossing between EGMS lines and other high yield cultivars. Heterosis study Our goals in this study are to1) evaluate the cross ability of male sterile lines under development (hereafter experimental EGMS) with different rice cultivars, and 2) observe synchronization between experimental EGMS and other cultivars. In 2018 our activities regarding the heterosis study is as follows: We will make crosses between BC1F5 Experimental EGMS plants and several selected high yield cultivars. The F1 seeds will be harvested and the quality and seed number from each cross will be recorded. The F1( hybrid) produced in summer 2017, will be grown in the field condition and evaluated for several agronomic traits such as plant height, plant type, heading dates, uniformity, panicle size, 10 plants yield, plot yield, shattering, lodging, milling and eating quality. Three line Method Developing Cytoplasmic Male Sterile (A) and Maintainer (B) lines Developing A and B lines is a time consuming process and the strategy of developing these lines are more complicated compare to two-line method. Like EGMS line, the A and B lines developed at RREC did not meet the agronomic characteristics e.g. heading date, which is very short, as well as eating qualities require for the hybrid production. To tackle these issues, we have selected two RREC B lines and crossed with several UA rice cultivars. These lines are being backcrossed with their correspondent B lines, then the BC1F1 will be grown in the field in summer 2018. The BC1F2 plants will be grown in winter nursery at Puerto Rico in winter 2018. Developing restorer (R) lines Majority of American rice cultivars do not carry the restorer genes. Our goal is develop new restorer lines that are long grain, high yield, semi-dwarf, disease resistance, non-aroma, medium amylose content, intermediate gelatinization temperature, and ideal heading dates. Currently, we have selected numbers of F2 R plants and grown in the field and greenhouse condition. The F3 seeds will be sent to the winter nursery early January and the F3 plants will be grown in the summer 2018. The restorability test will take place in summer 2018. We will continue to initiate developing new R lines by crossing UA R lines with selected cultivars or superior advanced lines. Improving Hybrid rice germplasm We continue collaborating with Dr.s Moldenhauer and Sha and introduce their advanced lines into our germplasm for developing new EGMS, A/B, R, and hybrid lines. This fall, we received several advanced lines from Dr. Moldenhauer's lab which will be used for developing male sterile, and hybrid lines. Lines received from Dr. Sha's lab will be used for developing R and B lines. Obtaining EGMS male sterile lines from outside is very difficult. Upon my arrival to UA, I made contact with IRRI, and finally after 20 months, we got a new TGMS (temperature genic male sterile) line "IR 75589-31-27-8-33". Presently, this line is under quarantine at UA Rosen Center. We occasionally travel to Fayetteville to check this line. The seeds from this line, which will be the new source of sterility, will be crossed with selected cultivars to develop new male sterile lines in spring/summer 2018 at greenhouse condition.
Impacts
What was accomplished under these goals?
Non-Technical Summary In hybrid breeding program, our focus has been to develop hybrid rice cultivars adapted to rice growing regions of the Southern U.S. which produce high grain and milling yields, have desirable plant type, exhibit resistance to prevalent diseases and possess typical U.S. long grain quality. Procedures for breeding and identification of hybrids, restorers and maintainers genotypes share similarities to those used for inbred line development. Developing male sterile lines for both 2- and 3- line male sterile systems is more complex, but basic breeding principles still apply. Once identified, characterization of the type of CMS system or EGMS genes is needed to consistently produce hybrid seed. A large number of testcrosses between potential restorers/elite cultivars and male-sterile lines will be needed to select the best hybrid cultivar. Allocation of time to seed production will be necessary to determine which combinations produce the best hybrid seed yields. Collaborative research efforts between the University of Arkansas hybrid rice breeding program and other faculty within the University of Arkansas system, as well as other domestic and international institutions will greatly aid our efforts. Accomplishments Major goals of the project Development of male-sterile systems (both 2- and 3-line), restorer (male) and maintainer (3-line system) lines adapted to the Southern U.S. rice growing region. Development of elite hybrid cultivars which have high grain and milling yields, desirable plant type, resistance/tolerance to biotic or abiotic stresses and typical U.S. long grain quality. Development of inbred lines in parallel with hybrid line development. Implementation and application of new marker technologies and methodologies to aid in the identification of marker-trait associations which be used as marker assisted selection to improve selection efficiency and increase productivity. Develop an effective and economical hybrid seed production system. Large scale mechanization to improve the efficiency of the hybrid rice breeding program. Fostering collaborative research agreements between the University of Arkansas hybrid rice breeding program and other faculty within the University of Arkansas system, as well as other domestic and international institutions. What was accomplished under these goals? TTwo-line System A total 191 lines from two BC1F4 populations resulting cross between 236s, an Environment Genic Male Sterile (EGMS) lines (experimental BC1F4 hereafter) developed by RREC, and RU1201102 were tested in field condition in summer 2017. Previously, these lines had been tested and selected based on good cooking quality and several agronomic traits via a molecular study. Each plot was evaluated based on phenotypic characteristics such as uniformity, heading date, plant height, plant type, number of panicle per plant, stiff straw, overall panicle exertion, seed size, and % sterility of first panicle. The selected lines were ratooned and seeds from each plot were bulked as BC1F5 seeds. A total 47 BC1F3 lines from four populations resulting from crosses between 236s and 811s, designated as female parents, with Francis, Cocodrie, and RU1201102, assigned as male parents, were grown in a field. These lines were derived from 47 BC1F2 plants which were evaluated via a molecular study for cooking quality, disease resistance, and semi dwarf. The BC1F3 single plants from these populations phenotypically were evaluated for heading date, plant type, plant height, sterility, and panicle exertion rate, plant height, and seed size. The selected BC1F3 plant were ratooned and transferred in a greenhouse to get BC1F4 seeds. More than 4100 F2 plants resulting from crosses between 236s, 805s, and 811s with 17 high yield, semi dwarf, and non-aroma cultivars or advanced lines were planted in greenhouse and field conditions and were tested for sterility. Of 4100 plants, more than 350 F2 plants were selected and tested for several agronomic traits such as eating characteristics, semidwarf, disease resistance etc. via a molecular study. The selected F2 plants were ratooned to produce F3 seeds which will be planted in summer 2018. We crossed two male sterile lines of 236s and 811s and several plants from experimental BC1F4 population with number of high yield, semi dwarf, and non-aroma cultivars or advanced lines to produce F1 (hybrid seeds). We collected F1 seeds from these crosses. The F1 plants will be grown and evaluated next year through a heterosis study in summer 2018. Number of crosses between 81s and 236s and high yield, semi dwarf, and non-aroma cultivars or advanced lines were made for developing new male sterile lines for two line hybrid system. The F1 seeds will be grown in the greenhouse to get F2 plants will be screened for their level of sterility. A total 33 hybrid population resulting from crosses, which were made in summer 2016, between 811s and experimental BC1F3 plants and several cultivars were grown in the field and evaluated via a heterosis study. The results revealed that the hybrids resulting from the experimental BC1F3 showed greater phenotypic characteristics than 811s hybrid lines. All experimental BC1F3 demonstrated shorter plant height, better stands, larger panicle size, greater stiff straw, better plant type, and no lodging compare the 811S hybrid lines. In addition, more than 35% of hybrid resulting from 811s severely logged. Moreover, number of hybrids produced more seed yield than checks, which were Diamond, Mars, Taggart, RoyJ, and Titan. Presently, we are in process of evaluating cooking and milling quality of these hybrid lines. Three-line System Number of crosses between UofA maintainer (B) and restorer (R) and high yield, semi dwarf, and non-aroma cultivars or advanced lines were made for developing B and R lines which will be used for three line hybrid production. The F1 plants will be grown in a greenhouse and will be crossed with their corresponded B and R line to get BC1F1 seeds. More than 2500 F2 single plants resulting from crosses between several UofA R lines and high yield, semi dwarf, and non-aroma cultivars or advanced lines were grown in a greenhouse and tested for several agronomic traits such as amylose content, disease resistance, and plant height. More than 400 F2 plants were selected. The F2 seeds were sent to the winter nursery and the F3 populations will be tested for ability of crossing with CMS lines in summer 2018. Screening RREC restorer and male sterile lines we conducted a molecular and phenotypic studies to evaluate and homogenize 14 EGMS and five R lines previously developed by RREC for cooking and agronomic traits. Several EGMS and R plants were selected, and their seeds were bulked. These lines will be used for developing new R and EGMS lines. MS Thesis Projects 1. Identification of Genetic Sources of Arkansas Male Sterile Lines, MS. Student: Mr. Adam Rice 2. Identification of QTLs associated with yield component in rice cultivar LaGrue Rice; MS student: Mr. Adam Rice 3. Identification of Restorer genes in Arkansas Restorer Lines,MS Student: Mr. Ozgur Azapoglu Research Collaboration 1. Application of Gibberellic Acid for male sterile panicle extension improvement.Collaborator: Dr. Paul Counce, Mr. Dustin North ,and Mr. Courtland Hemphill 3. Expression of gene associate with sterility in Arkansas male sterile lines, Collaborators: Dr. Ramesh Dhaka, Dr. Cassia Oliveira, and Mr. Dustin North What opportunities for training and professional development has the project provided? Our personnel have had this opportunity to learn about hybrid breeding and field, greenhouse, and laboratory management such as implementing and executing field study, pollen stain evaluation, planting, irrigating, spraying pesiticides, adding fertilizer, driving tractor and combine, recording several phenotypic characteristics etc. Presently, two master students have joined to our program working
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
E. Shakiba, J. Edwards,&, S. McCouch, and G. Eizenga. "Genetic architecture of cold tolerance in rice (Oryza sativa) determined through high resolution genome-wide analysis." PloS one 12, no. 3 (2017): e0172133
- Type:
Journal Articles
Status:
Awaiting Publication
Year Published:
2018
Citation:
G. Eizenga, M. Jia, A. Jackson,&, E. Shakiba, and J. Edwards. Validation of yield component traits identified by GWA mapping in a tropical japonica x tropical japonica rice mapping population. Plant Genome Journal
- Type:
Other
Status:
Published
Year Published:
2017
Citation:
V.A. Boyett, V.I. Thompson, E. Shakiba, & Impact of Molecular Analysis on Rice Breeding Efforts B.R. Wells Arkansas Rice Research Study 2016:pp 45-53.
- Type:
Other
Status:
Published
Year Published:
2017
Citation:
K.A.K. Moldenhauer, X, Sha, E. Shakiba,& Breeding and Evaluation for Improved Rice VarietiesThe Arkansas Rice Breeding and Development Program. B.R.Wells Arkansas Rice Research Study 2016:pp 65-74.
- Type:
Other
Status:
Published
Year Published:
2017
Citation:
X. Sha, K.A.K. Moldenhauer, E. Shakiba,& Development of Superior Medium-Grain and Long-Grain Rice Varietiesfor Arkansas and the Mid-South. B.R.Wells Arkansas Rice Research Study 2016:pp 75-8
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Progress 10/01/15 to 09/30/16
Outputs
Target Audience:Our targets are agronomic scientists, crop producers and industry, Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Our personnel have had this opportunity to learn about hybrid breeding and field, greenhouse, and laboratory management such as implementing and executing field study, pollen stain evaluation, planting, irrigating, spraying pesiticides, adding fertilizer, driving tractor and combine, recording several phenotypic characteristics etc. In addition, two master students have joined to our program How have the results been disseminated to communities of interest?Data was presented at the Arkansas Rice Expo and Field Days, Stuttgart, Arkansas. August 2016. What do you plan to do during the next reporting period to accomplish the goals?Continue working on my major goals.
Impacts
What was accomplished under these goals?
Two-line System Seeds from 49 BC1F3 plants resulting from a cross between 236s, an Environment Genic Male Sterile (EGMS) line developed by RREC, and RU1201102 were planted in 49 plots. Previously, these 49 BC1F3 plants had been tested and selected based on good cooking quality and several agronomic traits via a molecular study. Each plot was evaluated based on phenotypic characteristics such as uniformity, heading date, plant height, plant type, number of panicle per plant, stiff straw, overall panicle exertion, and % sterility of first panicle. A total 502 BC1F3 plants were selected, ratooned and placed in a greenhouse in an environmental condition required for seed production for male sterile plants. These plants are being tested for other agronomic traits to identify the most desirable plants among 502 BC1F3 plants for the next generation. The BC1F4 seeds from the selected plants will be planted in summer 2017 in a field condition and the selection will continue to develop a new male sterile line. To analyze percent of cross combination, several BC1F3 plants from the plots mentioned above along with 811s plants, a male sterile line developed by RREC, were crossed with Arkansas long grain cultivars or advanced lines. Overall there were 35 combinations with 811s and 26 combinations with the BC1F3 plants resulting in 61 new hybrid lines. The F1 (hybrid) seeds were carefully collected from each female (male sterile) plant. The F1 seeds (hybrid seeds) along with their male parental lines will be planted in a field condition in 2017 to evaluate hybrid performance. A total 1047 BC1F2 single plants from four populations resulting from crosses between 236s and 811s, designated as female parents, with Francis, Cocodrie, and RU1201102, assigned as male parents, were grown in a field and evaluated via molecular study for cooking quality, disease resistance, and semi dwarf, and phenotypically were evaluated for heading date, plant type, plant height, sterility, and panicle exertion rate. A total 40 plants were selected, ratooned and placed in a greenhouse to produce BC1F3 seeds which will be planted in a field condition in summer 2017. We crossed three male sterile lines of 236s, 805s, and 811s with 17 high yield, semi dwarf, and non-aroma cultivars or advanced lines and collected the F1 seeds resulting from 33 successful combinations. The F1 plants will be grown in a greenhouse condition this fall and backcrossed with their correspondent male sterile parents to get BC1F1 seeds which their plants will be grown in summer 2017. Three-line System We made crosses between 873B line, an Arkansas maintainer line, and 10 Arkansas elite cultivars or advanced lines. The F1 plants were collected and will be planted in a greenhouse and will be backcrossed with the B line to produce BC1F1 seeds. The BC1F1 plants will be grown in a field in summer 2017. One important part of our program to develop new restorer (R) lines because majority of American rice genotypes, which are Japonica cultivars, don't have R gene. Three restorer (R) lines of 351R, 367R, 394R, and 396R that possess genes associated with desirable traits were crossed with 39 high yield tall genotypes to develop new restorer lines. The F1 plants will be grown in a greenhouse and backcrossed with their correspondent R line to get BC1F1 seeds. The BC1F1 plants will be grown in a field condition in summer 2017
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2017
Citation:
Shakiba. E., J. Edwards, S. McCouch, G. Eizenga et al. (2016) Using Genome Wide Association Mapping (GWA)study to identify SNPs associated with rice seedling cold tolerance. Plos One
- Type:
Other
Status:
Published
Year Published:
2016
Citation:
Shakiba, E., K.A.K. Moldenhauer, G.L. Berger et al. 2016. Development of male sterile line for hybrid rice production In: R.J. Norman and K.A.K. Moldenhauer (edit.s), B.R.Wells Rice Research Studies 2016
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