Progress 04/10/14 to 09/30/18
Outputs
Target Audience:Shrimp broodstock breeders, aquaculture consultants, scientists in animal genetics and breeding, shrimp feed developer, aquaculture technology companies. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Laboratory research skills and copepods culture setup is important for us to continue training new students and developing new project for large-scale production. Our publications in DNA-based technology for aquatic species may attack more visiting scientists and students to come to Hawaii for training and research investigation. The other opportunity is the technology and set-up we developed may have commercial application for live feed production for shrimp hatchery farms. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
1. Evaluations of microsatellite DNA markers from P vannamei species and several highly polymorphic DNA markers have been identified for paternal parent or sire determinations. The results were reported in Plan and Animal Genome conferences in 2015. 2. Amid working on the project, my laboratory trained two visiting scholars. One of the visiting scholar collaborated with us and used genomics tools to study mitochondrial genomes and phylogenetic relationships of three croakers (Nibea coibor, Protonibea diacanthus and Argyrosomus amoyensis, Perciformes, Sciaenidae). All these fish important commercial species inhabiting the Eastern Indian Ocean and Western Pacific. By sequencing the complete mitochondrial genomes of the three croakers using next-generation sequencing, the results include 1) reports of complete mitochondrial genome sequences of the three croakers; 2) gene arrangement and distribution of the three croakers are canonically identical and consistent with other vertebrates; 3) the family Sciaenidae is an independent branch that is isolated from the order Perciformes and does not belong to any extant classification. One manuscript is published in 2018. 3. The perational size of Hawai'ian aquaculture production is highly dependent on the cost of imported feeds. Plankton is the natural food source for the larval stages of Litopenaeus vannamei the use of locally produced cultures as live feed in larval rearing stages could give the animals a healthy start while reducing the cost to the producer. One graduate student is interested in working shrimp hatchery and live feed technology development. The thesis project is planned to characterize the optimal feeding of local plankton species in larval rearing of the Pacific white shrimp L. vannamei. Copepods are the natural prey for most larval fishes, crustaceans and mollusks. When utilized in aquaculture systems as a live feed, copepods have increased survivability and growth rates. Nauplii of many copepod species are smaller than those of brine shrimp (artemia) currently used in many aquaculture systems on the Hawai'ian islands. The small size of copepod nauplii could be vital for the first feeding of larval species with small mouth gape sizes. Copepods also stimulate strong feeding responses in many larvae due to their distinctive, jerky swimming patterns. The tropical harpacticoid Euterpina acutifrons was chosen for the study because they are found in local waters and the ability to be cultured in mass quantities. Trials conducted with the Euterpina species and the locally produced shrimp Litopenaeus vannamei showed targeting of progressively larger copepod stages as the shrimp aged. The L. vannamei species was shown to be successful predators of all E. acutifrons life stages with vigorous ingestion of all developmental stages by 15 days post-hatch. By testing a pilot project, we accomplished several live feed (i.e. copepods) culture protocols and preliminary shrimp larvae rearing in the laboratory. Utilizing observational study, the findings of this thesis confirms that E. acutifrons is a high potential species for shrimp larviculture due to the continued capture success and preferences by L. vannamei. The E. acutifrons also had high culture productivity, adding to its advantages as a live feed. 4. One graduate student and 3-4 undergraduate students have been on the project, and they are excited and gain significant research and hand-on experiences in developing aquaculture techniques and production.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Yang, H., Xia, J., Zhang, J. E., Yang, J., Zhao, H., Wang, Q., Sun, J., Xue, H., Wu, Y., Chen, J., Huang, J., and Liu, L. 2018. Characterization of the Complete Mitochondrial Genome Sequences of Three Croakers (Perciformes, Sciaenidae) and Novel Insights into the Phylogenetics. International Journal of Molecular Sciences, 2018. 19(6), 1741.
- Type:
Theses/Dissertations
Status:
Other
Year Published:
2018
Citation:
Dahl, D. 2018. Investigation of Copepods as a Live Feed for Hawaii Larval Shrimp Culture. M.S. Thesis, (Jinzeng Yang as Advisor), University of Hawaii at Manoa, Honolulu, HI. 83 pp.
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Progress 10/01/16 to 09/30/17
Outputs
Target Audience:Shrimp breeder and bloodstock hatchery operators, aquaculture developers, scientists and students. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Training opportunities for graduate students and visiting scholars, and collaboration with local bloodstock shrimp industry, and continue to support SPF shrimp and other aquaculture development. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?1. Seeking more funding for the project and collaboration with shrimp breeding industry.
Impacts
What was accomplished under these goals?
1. We are still working on the bioinformatic data and genes related to shrimp growth and muscle mass. 2. Previous data on parental assignment has been reanalyzed and prepared for a report.
Publications
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Progress 10/01/15 to 09/30/16
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?Seek funding for shrimp biofloc technology and feed development in Hawaii.
Impacts
What was accomplished under these goals?
Development of collaborations with private shrimp farms for shrimp feed and hatchery operations.
Publications
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Progress 10/01/14 to 09/30/15
Outputs
Target Audience:Shrimp farmers, shrimp broodstock breeding companies, aquaculture consultants, animal geneticists, scientists and researchers in shrimp/aquaculture genomics. Changes/Problems:1. Costs are greater than anticipated relative to available funds. 2. Lack of shrimp culture facility and broodstock holding facility at the UHM also limited our ability to run grow-out trials. What opportunities for training and professional development has the project provided?Training for undergraduate and graduate students who are interested in animal science and aquaculture, and developing laboratory protocols for laboratory class and workforce training. How have the results been disseminated to communities of interest?Conference presentations and journal publications. What do you plan to do during the next reporting period to accomplish the goals?Based on the initial plan of work, we will continue the DNA-based testing for P vannamei parental assignment and gene expression analysis for growth trait selections.
Impacts
What was accomplished under these goals?
1. The project worked on the evaluations of microsatellite DNA markers from P vannamei species and several highly polymorphic DNA markers have been identified for paternal parent or sire determinations. The results were reported in Plant and Animal Genome conferences in 2015. 2. Two genes previously identified from P vannemei shrimp muscle have been studied and verified in the laboratory. By gene expression analysis of these genes and their relationship to growth traits, we expect to use these gene and analysis for growth traits selection.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2015
Citation:
Kuehu, D. and Yang, J. 2015. Intergrating low salinity acclimation of Pacific white shrimp Litopenaeus vannamei with environmental ionic and probiotic effects. World Aquaculture Society Meeting
(Fortaleza, Brazil, 2015)
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Miao, Y., Yang, J., Xu, Z., Jing, L., Zhao, S., and Li, X. 2015. RNA Sequencing Identifies Upregulated Kyphoscoliosis Peptidase and Phosphatidic Acid Signaling Pathways in Muscle Hypertrophy Generated by Transgenic Expression of Myostatin Propeptide. International Journal of Molecular Sciences. 16(4):7976-7994.
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Progress 04/10/14 to 09/30/14
Outputs
Target Audience: Shrimp farmers, shrimp broodstock breeding companies, aquaculture consultants, scientists of animal genomics and genetics, aquaculture technology companies. Changes/Problems: Limited funding and lack of on-site shrimp hatchery and broodstock breeding at the University of Hawaii at Manoa are main problems for this project. It will really help this project and other researchers of the university to develop shrimp aquaculture facilities. What opportunities for training and professional development has the project provided? This project has provided the opportunities for undergraduate students and graduate students to gain research skills and laboratory experiences in developing DNA-based technologies for aquaculture and animal agriculture. How have the results been disseminated to communities of interest? Yes, the results were presented in the international meeting of Plant and Animal Genome Conferences and student research symposium at the University of Hawaii. Also, we have shared the results with companies interested in developing DNA-based technologies for aquacultures. What do you plan to do during the next reporting period to accomplish the goals? Further develop DNA-based testing tools for vannamei parental assignment and validate the methods in different populations.
Impacts
What was accomplished under these goals?
P. vannameihas been bred for commercially valuable traits such as growth rate and disease resistance.However, selectively bred shrimp populations are susceptible to inbreeding. DNA-based testing can be used for pedigree tracking to control inbreeding. Microsatellite DNA markers have been an effective genetic tool for parental assignment. This research has evaluated 7 microsatellite DNA markers (Pvan 0013, Pvan 1003, Pvan 1758, Pvan1815,M1,TUGAPv 7-9.115, andTUGAPv 7-9.132) and determine the paternity of 9 families of shrimp progeny.All microsatellite markers were highly polymorphic, with more than 4 alleles and PIC of more than 0.5. The expected heterozygosities of these microsatellite markers,except M1, were also above 0.5. Among all microsatellites,Pvan 1758was the most highly polymorphic marker having the highest PIC value of 0.814, highest expected heterozygosity of 0.840, and the most number of alleles (k=7). The combined exclusion probabilities increased to 0.974 for known dam, and 0.874 for unknown both parents with all 7 microsatellites were evaluated together.
Publications
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