Progress 07/01/14 to 06/30/19
Outputs
Target Audience:Due to the nature of our projects, research questions are prioritized according to our stakeholder needs, thus our target audience primarily includes researchers, growers,industry and general public. More specifically we address scientific community (researchers (academia/industry, students, extension county educators, other professionals), individual growers, commodity commisions (Idaho Wheat Commission and Idaho Barley Commission) and Idaho agricultural industry in general (companies, Ag. consultants). Overall, community and general public are also expected to benefit from the outcomes of this study through improved food security. Changes/Problems:Due to the variability in wireworm data we extended our data collection into 2020 (and as a part of the new hatch proposal). However, a paper to report genetic variability among wireworm species in Idaho is prepared and will be submitted in upcoming days. What opportunities for training and professional development has the project provided?Through these project several researchers and students have been trained. That included one visiting scientist, one post doctoral students, three graduate students, one technician, and several undergraduate students. Team memebrs were trained on and contributed to field research, experimental design, data collection and analyses, and molecular techniques. They communicated their research and findings with growers and scientific community through various means incouding workshops, extension and research publications, as well as numerous oral and poster presentations. How have the results been disseminated to communities of interest?We continue to disseminate our findings to researchers and stakeholders through scientific journal publications, reports, conference meetings, symposiums, field days, Cereal and Potato schools, and commodity reporting sessions. My lab members, and myself, travel across the state on a regular basis visit grower fields regularly and interact with stakeholders in person. This will help to understands, issues to prioritize is our research. As listed in Target Audience section our audiences include scientific community, growers, industry, and students attending annual conferences. What do you plan to do during the next reporting period to accomplish the goals?This is a final report of this proposal. However, our findings over the past five years opened new avenues to investigate. New questions formed the bases for a new hatch proposal that is currently ongoing. For this proposal, we have addressed all of our proposed objectives and are finalizing results for a few of the studies. I will publish our BYDV findings in upcoming months. We will also continue to collect ecological data on wireworms, while we publish our distribution map of different species, a manuscript is currently at final stage of preparation, and is being reviewed by coauthors.
Impacts
What was accomplished under these goals?
1. This goal has been completed and results have been presented in various meetings. We found no variations insusceptibility of commonly planted winter wheat cultivar to BYDV infection. However, differential preference for wheat cultivars was reported in bird cherry-oat aphid. As the BYDV outbreaks continued, we developed new objective; we have identified several BYDV reservoirs in the region and their role as overwintering hosts of aphid and the virus. We have identified five wild grasses as reservoirs of the virus. We also confirmed that aphid vectors are capable of acquiring the virus from these host and transmitting it to winter wheat. Results have been presented in various extension meetings, workshops and seminars. Corn appears to also be a crop that can contribute to BYDV outbreak. In the recently submitted proposal we are evaluating the effects of stress on aphid reproduction on these wild hosts. We also demonstrated that the virus over winters in the perennial foxtail barley in the region. Goal 2: This objective is mostly completed. However, we are still analyzing newly collected data to develop a distribution map for various species of wireworms multiple years of data is needed for this purpose due to the very uneven distribution of wireworms. We are including northern Idaho in our analysis to increase the power to detect differences and patterns. Our monitoring work of wireworm species is now one of the largest studies being conducted in the US. Goal 2: A five-year dataset has been prepared and we are in the process of handling (thousands of data points) and analyzing data; this has been a continuous and ong process since constantly new patterns with high levels of variability are being identified (our survey is entering its sixth year). To date, we have shown that the sugar beet wireworm Limonius californicus is the most damaging and commonly found species in Idaho. This species is most commonly found in sand-dominated soil. Damage by this species is also higher when there is sandy soil. No-till practice does not seem to reduce Aeolus mellillus numbers but seems to reduce wireworm pressure if L. californicus is the main pest; this has yet to be confirmed in more fields. Barely is a more tolerant crop than wheat. However, this tolerance is not due to higher number of tillers. To date, this project has generated two paper on the impact of soil type and crop susceptibility to wireworm damage and results are being continuously reported to researchers and growers. In addition, to date,we have studied the efficacy of biological control and crop rotation against wireworms. We also compared susceptibilities of high tillering and low tillering wheat cultivars to wireworm damage. Goal 4: Results were finalized and a paper was officially published in 2018. We showed, for the first time, that Liberibacter continues to interact with harvested tubers during different stages of commercial storage and that there are variations in Lso-tuber interactions with respect to time of infection during the field season. A summary of findings was included in the previous report. Overall, while tubers collected from plants which were infected only 4 days before harvest all tested negative for the bacteria at harvest, a considerable percentage of the same tubers tested positive following a few months of storage. Thus, the pathogen arrives into the tuber tissue only days (if not, hours) after inoculation by the vector and remains at undetectable levels, even with sensitive qPCR. New objectives have been developed and we are now investigating potential sources of resistance to ZC in more than 25 new, and also commercially available germplasm.
Publications
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Rashidi, M.*, Cruzado, R.K.*, Nikoukar, A.*, Hutchinson, P.J.S., Marshall, J., Bosque-Perez, N.A., Rashed, A. Barley yellow dwarf virus transmission by the bird cherry-oat aphid Rhopalosiphum padi L. (Hem., Aphididae): Can corn and grassy weeds contribute to regional BYDV outbreaks? Entomological Society of America, Entomological society of America Annual Meeting. Vancouver, BC, November 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Nikoukar, A.*, Ensafi, P., Bosque-Perez, N.A., Lewis, E., Rashed, A. Efficacy evaluations of commercial and field collected entomopathogenic nematodes against wireworms. Biological Control W-4185 Annual Meeting. Whitefish, MT, October 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Cruzado R.K.*, Liang, X., Bosque-P�rez, N.A., Rashed, A. Effect of Candidatus Liberibacter solanacearum on potato host and its potato psyllid vector, Bactericera cockerelli (Hemiptera: Triozidae). Poster. Pacific Branch Entomological Society of America. Reno, NV, June 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Rashed, A. Invited talk: Wireworm Management: Research update and current recommendations. 2019 Far West January Winter conference. Twin Falls, ID, January 2019.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
Cruzado R.K., Bosque-P�rez N.A., Prager S.M., Casteel C.L. and A. Rashed. Plant-mediated effects of potato virus Y on the zebra chip pathosystem. Internation Plant Virus Epidemiology Symposium.
|
Progress 10/01/17 to 09/30/18
Outputs
Target Audience:As research questions are prioritized accroding to our stakeholder needs out target audience includes researchers, growers, industry and general public. More specifically: • Scientific community (researchers (academia/industry, students, extension county educators, other professionals) • Individual growers • Commodity commisions, including: Idaho Wheat Commission and Idaho Barley Commission • Idaho agricultural industry in general (companies, Ag. consultants) • Community and general public will also benefit from the outcomes of this study through food security Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?• A visiting scientist from China was trained throughout 2017 and left in January 2018. A paper was generated from his efforts and published in the American Journal of Potato Research. In this study, we used infrared thermal imaging and visual and near infrared wavelengths to detect and quantify ZC symptoms in infected tubers a technology that is expected to help US potato industry. Through that work we proposed a new measure to quantify ZC symptoms that is expected to help with data analysis in this and perhaps other systems. • This project trained one post-doctoral student on various aspects of experimental design, pathogen quantification, data analysis, and data presentation. She was provided with the opportunity to be trained in other laboratories for various molecular techniques involving Liberibacter and BYDV. She is currently holding a postdoctoral position at the University of Florida. She published one paper and coauthored several others. A new post doctoral researcher has been hired through an SCRI project and is helping with some of our objectives in this proposal occasionally. • One PhD student has been trained on molecular approached in BYDV and Zebra Chip projects. She now has one manuscript in preparation. She also attended two conferences and her work was also presented in various meetings. Moreover, one master's student has completed her projects and is expected to graduate in May. She has been working on the biological control of wireworms and identifying endosymbionts associated with L. californicus. She is now trained on various laboratory techniques including DNA extraction, cPCR and qPCR. • I have established several new collaborations with researchers from different institutions including scientists in Canada and China • I continued my collaborations with more than 32 growers in Southern Idaho. More farmers are interested to join our program. This project is now expanded to northern Idaho. We are hoping to provide the same service to our Ag industry in northern Idaho. Idaho cereal growers and commodity commissions have provided exemplary support for our research program. How have the results been disseminated to communities of interest?We continuously disseminate our findings to researchers and stakeholders through scientific journal publications, reports, conference meetings, symposiums, field days, Cereal and Potato schools, and commodity reporting sessions. My lab member, and myself, visit grower fields regularly and interact with stakeholders in person. this will help to understand issues, to prioritize is our research. As listed in Target Audience section our audiences include scientific community, growers, industry, and students attending annual conferences. What do you plan to do during the next reporting period to accomplish the goals?We have addressed all of our proposed objectives and are finalizing results for a few of the studies. I will publish our BYDV findings in upcoming months. As 2019 season is about to start, we will continue to collect ecological data on wireworms, while we publish our distribution map of different species. Cultural and ecological data will be analyzed for individual species to identify species-specific control measures. This hatch proposal is nearing its end, and we have developed new objectives on similar pest conditions (vector of Liberibacter (ZC system), wireworms, and BYDV) to continued our research on some of the most cncerning pest issues in the state of Idaho.
Impacts
What was accomplished under these goals?
Goal 1: This goal has been completed and results have been presented in various meetings. We found no variations in susceptibility of commonly planted winter wheat cultivar to BYDV infection. However, differential preference for wheat cultivars was reported in bird cherry-oat aphid. As the BYDV outbreaks continued, we developed new objectives in this particular pathosystem. We are identifying BYDV reservoirs in the region and evaluating their role as overwintering hosts of aphid and the virus. To date, we have identified five wild grasses as reservoirs of the virus. We completed several transmission assays to confirm the status of three of the plant species as sources of infections in cultivated fall-planted cereals. We compared aphid reproduction, as well as BYDV acquisition and transmission success in the presence of these alternative hosts. Results have been presented in various extension meetings, workshops and seminars. Corn appears to also be a crop that contributes to BYDV epidemics, especially at early developmental stages. we have continued monitoring of the aphids and their movement between various reservoirs and cultivated winter cereals. Goal 2: This objective has been completed and data is currently being analyzed to develop a distribution map for various species of wireworms. Currently, we are evaluating genetic variability, and variation in endosymbiont populations, in sugar beet wireworms across the state. Among-population variations in genotype and endosymbiont compositions may explain differences in susceptibility to neonicotinoids. Our monitoring work of wireworm species is now one of the largest studies being conducted in the US. Goal 3: This objective is near completion. To date, we have shown that the sugar beet wireworm Limonius californicus is the most damaging and commonly found species in Idaho. Thus, our research is now focused on its management. A four-year dataset has been prepared and we are in the process of handling (thousands of data points) and analyzing data; this has been continuous and a long process since constantly new patterns with high levels of variation are being identified (our survey is entering its fifth year). Upon detecting new patterns, we would need to test their effects under controlled conditions as we proceed. This project has generated one paper on the impact of soil type and crop susceptibility to wireworm damage (previously see list of publications) and results ar being continuously reported to researchers and growers. In addition, to date, we have studied the efficacy of biological control and crop rotation against wireworms. We also compared susceptibilities of high tillering and low tillering wheat cultivars to wireworm damage. We continuously find the importance of soil texture in the extent of damage by wireworms- Wireworms appear to cause more damage in sandy soil. Goal 4: Results were finalized and a paper was officially published in 2018. We showed, for the first time, that Liberibacter continues to interact with harvested tubers during different stages of commercial storage and that there are variations in Lso-tuber interactions with respect to time of infection during the field season. A summary of findings was included in the previous report. Overall, while tubers collected from plants which were infected only 4 days before harvest all tested negative for the bacteria at harvest, a considerable percentage of the same tubers tested positive following a few months of storage. Thus, the pathogen arrives into the tuber tissue only days (if not, hours) after inoculation by the vector and remains at undetectable levels, even with sensitive qPCR. New objectives have been developed and we are now investigating potential sources of resistance to ZC in more than 25 new, and also commercially available germplasm. Field and greenhouse data are currently being analyzed as a part of a graduate student project.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Zhao, Z., S.M. Prager, R.K. Cruzado, X. Liang, W.R. Cooper, G. Hu & A. Rashed. 2018. Characterizing zebra chip symptom severity and identifying spectral signatures associated with Candidatus Liberibacter solanacearum-infected potato tubers. American Journal of Potato Research, in press.
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Rashed, A., N. Olsen, C.M. Wallis, L. Paetzold, L. Woodell, M. Rashidi, F. Workneh & C.M. Rush. 2018. Post-harvest development of Candidatus Liberibacter solanacearum and its impact on the late-season infected potato tubers, with emphasis on cold storage procedures. Plant Disease, 102:561-568.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Rashed, A., Cruzado, K., Wenninger, E., Olsen, N., Karasev, A., Wharton, P. Zebrachip and relative susceptibility of potato cultivars in Idaho. Idaho Potato Conference (presented in Zebra Chip Session). Pocatello, ID, January 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2018
Citation:
Cruzado R.K., Liang, X., Bosque-P�rez, N.A., Rashed, A. Effect of Candidatus Liberibacter solanacearum on potato host and its potato psyllid vector, Bactericera cockerelli (Hemiptera: Triozidae). Poster. Pacific Branch Entomological Society of America. Reno, NV, June 2018.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Nikoukar, A., Ensafi, P.*, Crowder, D., Esser, A., Marshall, J.M., Rashed, A. Ongoing research on wireworm ecology and management. Idaho Association of Plant Protection. Twin Falls, ID, November 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Zhao, Z.*, S.M. Prager, R.K. Cruzado*, X. Liang, W.R. Cooper, G. Hu, Rashed, A. Using spectral signatures to quantify zebra chip disease severity and Candidatus Liberibacter solanacearum quantity in infected potato tubers. Remote Sensing Conference. Madison, WI, November 2017.
|
Progress 10/01/16 to 09/30/17
Outputs
Target Audience:Specific target audience would include: • Scientific community (in forms of scientific papers, seminars, and national and international conference presentations) • Individual growers (field visits when requested, field days, schools) • Idaho Wheat Commission (reports and presentations) • Idaho Barley Commission (report and presentations) • Idaho Potato Commission (report and presentations) • Idaho agricultural industry in general (companies, Ag. consultants); (reports and presentations, one-on-one interactions) • Community and general public will also benefit from the outcomes of this study through food security and availability of nutrition sources with improved quality; this would include people from ethnic minorities as well as the economically disadvantaged members of the community. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?• A visiting scientist (listed as post doc in this report) from China was trained throughout 2017. We have now submitted one paper for publication. There, we used infrared imaging and visual and near infrared wavelengths to detect and quantify ZC symptoms in infected tubers a technology that is expected to help US potato industry. We are now hoping to do additional experiments to detect more variables that may be used for disease quantification and prediction of symptom severity throughout processing stage (chipping). • A post-doctoral student was trained on various aspects of experimental design, pathogen quantification, data analysis, and data presentation. She was provided with the opportunity to be trained in other laboratories for various molecular techniques involving Liberibacter and BYDV. After three years in my laboratory, she completed her term in April 2017 and moved on to continue her research at the University of Florida. She published one paper and coauthored several others. She is now drafting another manuscript based on her work in my lab. • One PhD student has been trained on molecular approached in BYDV and Zebra Chip projects. She now has one manuscript in preparation. She also attended two conferences and her work was also presented in various meetings. Moreover, one new graduate student (master degree) joined my laboratory. She is now being trained on DNA extraction and quantification and is expected to work on wireworm population genetics and endosymbionts. She also had the opportunity to attend a meeting this fall (Entomological Society of America Annual Meeting) • I have established several new collaborations with researchers from different institutions including scientists in Canada and China. • I continued my collaborations with more than 35 growers in Southern Idaho. More farmers are interested to join our program. Their contribution and involvement has been has assisted us to generate many results which are of interest to the scientific community. How have the results been disseminated to communities of interest?We continued to communicate our findings through reports, conference meetings, symposiums, field days, Cereal and Potato schools, and commodity reporting sessions. Growers were also provided with findings in their individual fields. Our audiences include scientific community, growers, industry, and students attending annual conferences. What do you plan to do during the next reporting period to accomplish the goals?We will continue our data collection for all of the 4 proposed objectives and also the new objectives which had been developed through our findings in the past four years. While my target was to publish two papers during 2017, we managed to publish 6 papers for this reporting period. At least, two manuscripts are expected to be submitted for publication for the next reporting. I will continue to collect ecological data for the wireworm project and to develop heat maps for different species, especially for the sugar beet wireworm, which is identified as the most damaging species in Idaho. Additional ecological data will be collected for the new objectives that are set to evaluated ecological and biological management approaches as component of integrated pest management. Susceptibility of the several potato germplasm developed through our USDA-ARS breeding program in Aberdeen (and also some commercial genotypes) to Liberibacter infection will be evaluated both during the growing season and throughout storage. While most of the field studies have been completed we are now conducting our evaluations throughout the storage. As our findings indicated that the disease continues to impact potatoes post-harvest, studies to identify sources of resistance must conduct screening until after storage.
Impacts
What was accomplished under these goals?
Goal 1: To quantify susceptibility and physiological responses of Idaho wheat cultivars, to bird cherry-oat aphid infestations and Barley yellow dwarf virus (BYDV) infection, at different plant developmental stages: Results have been presented in various meetings. Overall, no variations in susceptibility to BYDV infection was reported among cultivars. The Bird Cherry-Oat aphid vectors, however, showed relative variations in their preference for different wheat cultivars. A greenhouse study was completed on the timing of BYDV infections and plant damage. Results were analyzed- Some inconsistencies were present which needed further investigations. Moreover, additional objectives have been developed; results are presented in meetings. The new objectives include identifying BYDV reservoirs in the region and evaluating their role in BYDV epidemics. We detected three local reservoirs of the virus and completed several transmission assays to confirm their status as sources of infections in winter wheat. Results have been presented in various extension meetings and seminars. Corn appears to also be a crop that contributes to BYDV epidemics, especially at early developmental stages. A manuscript on this new objective is currently under preparation. This work also involves monitoring aphid movement between wild hosts and cultivated crops. As such, we are working with farmers in several areas to collect this information in their fields. Goal 2: To plot a distribution map and construct a visual identification key to the wireworm species present in south central and eastern Idaho: This objective has been completed (with the exception of the heat-map). We also helped other states to develop a similar approach. A new objective has also been developed in our wireworm project and we are currently evaluating genetic variability at the population level across the state. Among population variations may explain susceptibility to neonicotinoid seed treatments. Cereal growers have had a critical role in the success of this project, which is now one of the largest studies conducted in the US. Goal 3: To quantify ecological parameters of the most prevalent wireworm species and to establish species-specific integrated management approaches based on key behavioral/ecological traits: We continued collecting data on soil characteristics (texture, organic matter, pH, density) to identify factors predicting wireworm damage/presence. We showed that the sugar beet wireworm Limonius californicus is the most damaging species in Idaho, and thus, our research is now focused on its management. A three-year data set has been prepared and we are in the process of handling (thousands of data points) and analyzing data; this has been a long process since constantly new patterns are being identified. Upon detecting new patterns, we would need to test their effects under controlled conditions as we proceed. This project has generated one paper on the impact of soil type and crop susceptibility to wireworm damage (previously see list of publications) and results were reported last year. This year we tested whether wireworm damage is affected by tiller numbers; no effect was found. We also completed a greenhouse study to evaluate the efficacy of biological control on wireworm management a manuscript draft is now at the final stages of preparation for submission. In this study, we showed that the efficacy of entomopathogenic nematode and fungus is affected by soil type. Soil, rich in organic matter provided a suitable environment for fungi and improved their performance. Nematodes appeared to be more effective in reducing wireworm damage in sand-dominated soil. Goal 4: To quantify Liberibacter development and associated tuber biochemical responses at different stages of curing, ramp down, and 45°F storage in relation to the time of plant infestation and the number of bacteriliferous psyllids: Two-year data was collected, analyzed and presented in various meetings. Results were finalized and are now published in an international journal. Results were novel as we showed, for the first time, that Liberibacter continues to interact with harvested tubers during different stages of storage and that there are variations in Lso-tuber interactions with respect to time of infection. Tubers collected from plants which were infected only 4 days before harvest all tested negative for the bacteria at harvest. A considerable percentage of the same tubers, however, tested positive following storage. This indicated that the pathogen would arrive to the tuber tissue shortly after inoculation by the vector and remains at undetectable levels. It was previously thought that it would take about a week for the pathogen to arrive into tuber tissue. Based on our findings, specific recommendations have been provided to growers. New objectives have been developed and we are now investigating potential sources of resistance to ZC in more than 25 new, and also commercially available germplasm. Field and greenhouse data are currently being collected. We also published a part of our findings. Indeed, we are close to introduce three tolerant genotypes). One more publication is currently in preparation.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2018
Citation:
Rashed, A., N. Olsen, C.M. Wallis, L. Paetzold, L. Woodell, M. Rashidi, F. Workneh & C.M. Rush. 2017. Post-harvest development of Candidatus Liberibacter solanacearum and its impact on the late-season infected potato tubers, with emphasis on cold storage procedures. Plant Disease, in press.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Farrokhzadeh, H., Moravvej, G., Modarres Awal, H., Karimi. J. & A. Rashed. 2017. Comparison of molecular and conventional methods for estimating parasitism rate in pomegranate aphid Aphis punicae (Hem., Aphididae). Journal of Insect Science, 17: 110.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
" Beatty, C.D., M. S�nchez Herrera, J. H. Skevington & A. Rashed, H. Van Gossum, S. Kelso, T. N. Sherratt. 2017. Biogeography and systematics of endemic island damselflies: the Nesobasis and Melanesobasis (Odonata: Coenagrionidae) of Fiji. Ecology and Evolution 7: 71177129
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Rashidi, M.*, R. Novy, C. M. Wallis & A. Rashed. 2017. Characterization of host plant resistance to zebra chip disease from species-derived potato genotypes and the identification of new sources of zebra chip resistance. PLoS ONE, 12: e0183283.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Marshall, J.M., Rashidi*, M., Jackson, C. & A. Rashed. 2017. Seed and foliar treatments to reduce impacts of Barley yellow dwarf virus (BYDV). Plant Disease Management Reports, in press.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Wallis, C.M., A. Rashed, F. Workneh, L. Paetzold & C.M. Rush. 2017. Effects of holding temperatures on the development of zebra chip symptoms, Candidatus Liberibacter solanacearum titers, and phenolic levels in Red La Soda and Russet Norkotah tubers. The American Journal of Potato Research 94: 334-341.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Rashed, A., C.W. Rogers, M. Rashidi, & J.M. Marshall. 2017. Sugar beet wireworm Limonius californicus damage to wheat and barley: Evaluations of plant damage with respect to soil media, seeding depth, and diatomaceous earth. Arthropod-Plant Interactions, 11: 147-154.
- Type:
Books
Status:
Published
Year Published:
2017
Citation:
Eigenbrode, S., Bechinski, E., Bosque-Perez, N., Crowder, D., Rashed, A., Rondon, S. & Stokes, B. 2017. Insect Management Strategies. In: Advances in dryland farming in the inland Pacific Northwest. (eds. G. Yorgey and C. Krugner). Pp. 587. Washington State University.
- Type:
Other
Status:
Published
Year Published:
2017
Citation:
Rondon, S. I., Vinchesi, A., Rashed A., Crowder, D. 2017. Wireworms: A pest of monumental proportions. OSU Extension Service. EM 9166.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Rashidi, M.*, Wenninger E.J., Liang, X., Olsen, N., Karasev, A., Wharton, P., Rashed, A. 2017 Zebra chip symptom and Lso development in potato with respect to vector density and the time of infestation: A greenhouse evaluation. P: 118. 3rd Hemiptera-Plant Interaction Symposium. ICA-CSIC, Madrid Spain.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Wenninger, E.J., Rashed, A., Olsen N., Wharton, P., Karasev A. 2017 Effects of vector density and time of infection on zebra chip disease development both at harvest and during storage. P. 116. 3rd Hemiptera-Plant Interaction Symposium. ICA-CSIC, Madrid Spain
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Novy, R., Rashidi, M.*, Rashed, A. Screening of Solanum species-derived germplasm for resistance to the transmission of zebra chip disease. European Association of Potato Research Triennial Conference. Versailles, France, July 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Cruzado, R.K.*, Rashidi, M.*, Olsen, N., Wenninger, E.J., Novy, R.G., Bosque-Perez, N., Rashed A. Identification of resistance to zebra chip disease in species-derived germplasm for use in the development of zebra chip resistant cultivars for the U.S. National Association of Plant Breeders Meeting. University of California, Davis, CA, August 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Fife, A., Wenninger, E.J., Novy, R.G., Rashed, A., Bosque-Perez, N. Observing the settling behavior of the potato/tomato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae) on different potato (Solanum tubersum) germplasm. Pacific Branch Entomological Society of America. Portland, OR, April 2017
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Cruzado, R.K.*, Olsen, N., Wenninger, E.J., Novy, R.G., Bosque-Perez, N., Rashed, A. Screening for resistance to zebra chip in potato germplasm. Pacific Branch Entomological Society of America. Portland, OR, April 2017.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Rashed, A., Rashidi, M., Cruzado, K., Olsen, N., Wenninger, E., Bosque-Perez, N., Novy, R. Zebra chip resistance in potato: Building upon our current knowledge, shaping the future. Pacific Branch Entomological Society of America. Portland, OR, April 2017
|
Progress 10/01/15 to 09/30/16
Outputs
Target Audience:Specific target audience would include: Scientific community individual growers Idaho Wheat Commission Idaho Barley Commission Idaho Potato Commission Idaho agricultural industry in general(companies, Ag. consultants) Community and general public will also benefit from the outcomes of this study byhaving nutrition sources with greater higher quality; this would include people from ethnic minorities as well as the economicallydisadvantaged members of the community. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? A visiting scientist from China is joining my laboratory in upcoming days for the duration of 2017. He will be trained on molecular approaches to identify BYDV and Liberibacter, and will also be trained on wireworm species identification. A post-doctoral student has been trained on various aspect of experimental design, pathogen quantification, data analysis, and data presentation. She was provided with the opportunity to be trained in other laboratories for various molecular techniques involving Liberibacter and BYDV. She is currently working in transmission studies to evaluate the role of wild host plants in BYDV epidemics. The post-doctoral student attended one international meeting (APS), which provided her with the opportunity to interact withwide range of researchers. A PhD student has been trained on molecular approached employed in BYDV and Zebra Chip projects. She also had theopportunity to be trained in other labs on new techniques that she will continue to develop in my program. I have established collaborations with researchers from different institutions. I established direct collaborations with more than 35 growers in Southern Idaho. I continue to receive requests from growersto participate in our ongoing monitoring program. Their contribution and involvement has been exemplary. How have the results been disseminated to communities of interest?Similar to the previous years, our results have been communicated through reports, conference meetings, symposiums, field days, Cereal and Potatoschools, and commodity reporting sessions. Growers were also provided with findings in their individual fields. Our audiences include scientific community, growers, industry, and students attending annual conferences. What do you plan to do during the next reporting period to accomplish the goals?We will continueour data collection for all of the 4 proposed objectives and also the new two objectives which had been developed through our findings in the past three years. Iwill also prepare two manuscripts for publication in 2017 reporting period. Our ecological analyses of the wireworm project data will be completed in 2017. I will continue collecting data to develop heat maps for different species. Additional ecological data will be collected for a set of new objectives related to stress factors such as drought. Susceptibility of more than 20potato germplasm developed through our USDA-ARS breeding program in Aberdeen(and also some commercial genotypes) to Liberibacter infection will be evaluated both during the growing season and throughout storage.
Impacts
What was accomplished under these goals?
Goal 1:To quantify susceptibility and physiological responses of Idaho wheat cultivars, to bird cherry-oat aphid infestations and Barley yellow dwarf virus (BYDV) infection, at different plant developmental stages: Data collection was completed. Results have been presented in various meetings. A manuscript is currently under preparation to be submitted in 2017. Overall, no variations in susceptibility to BYDV infection was reported among cultivars. The Bird Cherry-Oat aphid vectors, however, showed relative variations in their preference for different wheat cultivars. A greenhouse study has been completed on the timingof BYDV infections and plant damage. qPCR analysis of the collected samples are completed and results are being evaluated and analyzed. Additional objectives have been developed. The new objectivesincludeidentifyingBYDV reservoirs in the region and evaluating their role BYDV epidemics. Goal 2:To plot a distribution map and construct a visual identification key to the wireworm species present in south central and eastern Idaho: This year we identified two additional species of wireworms in Idaho- To date, a total of 10 pest species of wireworms have been reported, through this project. The objective of generating a visual key for Idaho species was completed last year. This detailed document is currently available tostakeholder and researchers. We also produced a one-page visual key for distribution among all grower in different venues. We are currently in the process of generating a 'heat map' for different species. Additional data would be needed to achieve this objective, as such we will continue to collect wireworm samples from different locales across the state. Goal 3: To quantify ecological parameters of the most prevalent wireworm species and to establish species-specific integrated management approaches based on key behavioral/ecological traits: We continuedcollectingdata on soil characteristics (texture, organic matter, pH, density) to identify factors predicting wireworm damage/presence. A two-year data set has been prepared and we are in the process of handling (thousands of data points) and analyzing data. We are hoping to have at least one publication submitted by the end of next reporting period. We also completed a greenhouse study to evaluate potential ecological variables that may impact wireworm damage. Whileseeding depth did not appear to be a significant factor, soil type and crop type showed some impact. Barley appeared to bemore tolerant to damage than wheat. This work was accepted for publication in 2016 and is expected to be published in 2017. Goal 4:To quantify Liberibacter development and associated tuber biochemical responses at different stages of curing, rampdown, and 45°F storage in relation to the time of plant infestation and the number of bacteriliferous psyllids: Two-year data was collected, analyzed and presented in various meetings. Results are now submitted for publication. Results were novel as we showed, for the first time, that Liberibactercontinues to interact with harvested tubers during different stages of storage and that there are variations in Lso-tuber interactions with respect to time of infection. Tubers collected from plants which wereinfected only 4 days before harvest all tested negative for the bacteria at harvest. A considerable percentage of the sametubers, however, tested positive following storage. This indicated that the pathogen would arrive to the tuber tissue shortly after inoculation by the vector andremains at undetectable levels. It was previously thought that it would take about a week for the pathogen to arrive into tuber tissue. Based on our findings,specific recommendations have been provided to growers. New objectives have been developed and we are now investigating potential sources of resistance to ZC in more than 25 new, and also commercially available germplasm. Field and greenhouse data are currently being collected. A manuscript is expected to be submitted by the end of the next reporting period.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2017
Citation:
Rashed, A., C.W. Rogers, M. Rashidi, & J.M. Marshall. Sugar beet wireworm Limonius californicus damage to wheat and barley: Evaluations of plant damage with respect to soil media, seeding depth, and diatomaceous earth. Arthropod-Plant Interactions, in press.
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Rashed, A., C.M. Wallis, L. Paetzold, F. Workneh & C.M. Rush. 2016. Variations in zebra chip disease expression and tuber biochemistry in response to vector density. Phytopathology, 106: 854-860.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2016
Citation:
Rashed, A., Rashidi, M., Cruzado, R.K., Novy, R., Olsen, N., Wenninger, E. Identification of Resistance to zebra chip in species-derived germplasm and non- U.S. cultivars to develop zebra chip resistance potato cultivars. Pacific Branch Entomological Society of America, Honolulu, HI, April 2016.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2016
Citation:
Rashed, A., Rashidi, M., Rogers, C., Marshall, J.M. Wireworm damage to wheat and barley in relation to soil media and seeding depth. Pacific Branch Entomological Society of America, Honolulu, HI, April 2016.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2016
Citation:
Rashidi, M., Marshall, J.M., Bosque-Perez, N., Rashed, A. Barley yellow dwarf incidence and the bird cherry-oat aphid preference in four wheat varieties in Idaho. Poster. American Phytopathology Society Annual Meeting. Tampa, FL, July 2016.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2016
Citation:
Rashidi, M., Marshall, J.M., Bosque-Perez, N., Rashed, A. Barley yellow dwarf incidence and the bird cherry-oat aphid preference in four wheat varieties in the USA. International Plant Virus Epidemiology Symposium. Avignon, France, June 2016.
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Gerritsen, A.T., D.D. New, B.D. Robison, A. Rashed, P. Hohenlohe, L. Forney, M. Rashidi, C.M. Wilson, & M.L. Settles. 2016. Full mitochondrial genome Sequence of the sugarbeet wireworm Limonius californicus (Coleoptera: Elateridae), a common agricultural pest, ASM Genome Announcements, 4: e01628-15.
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Workneh, F., L. Paetzold, A. Rashed & C.M. Rush. 2016. Population dynamics of released potato psyllids and their Bacteriliferous status in relation to disease incidence in caged field plots. Plant Disease, 100: 1762-1767.
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Progress 10/01/14 to 09/30/15
Outputs
Target Audience:Target audience included: -Research scientists in the fields of potato production, potato disease, vectore-borne plant pathogens, and cereal pests -Graduate students and researchers through invited departmental seminars and conference presentations -Internationally presented to potato production industry -Potato growers in Idaho (e.g. through various extension venues including field days, and Idaho Potato School) -Alfalfa growers in eastern Idaho (a series of presentations in forage schools) -Cereal growers in Eastern Idaho (e.g. cereal schools, field days, extension publications) Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?-A post doctoral student has been trained on various aspect of experimental design, pathogenquantification, data analysis, and data presentation. She was provided with the opportunity to be trained in other laboratoriesfor various molecular techniques involving Liberibacter and BYDV. -The post doctoral studentattended different international meeting, which provided her with the opportunity to interact with widerange of researchers. -A PhD student has been trained on molecular approached employed in BYDV and Zebra Chip projects. She also had the opportunity to be trained in other labs on new techniques that she will continue to develop in my program. -I have established collaborations with researchers from different institutions. -I established direct collaborations with more than 28 growers in Southern Idaho. I continue to receive requests from growers to participate in our ongoing monitoring program. Their contribution and involvement has been exemplary. How have the results been disseminated to communities of interest?Our results have been communicated through reports,conference meetings, symposiums, field days, Cereal and potato schools, andpest reporting sessions. We even contacted growers individually to discuss findings in their fields. As mentioned earlier our audiences include growers, industry, researchers, and students attendingannual conferences. What do you plan to do during the next reporting period to accomplish the goals?We continued our data collectionfor all of the 4 proposed projects. While some of the objectives have been already completed, we have developed new study questions based on our findings. We will continue our data collection and press forward to publish our findings as journal articles. As projected in the last year report, data collection for the following has been completed. -To quantify susceptibility and physiological responses of Idaho wheat cultivars, to Rhopalosiphum padiinfestations and Barley Yellow Dwarf Virus infection, at different plant developmental stages- we are working to run a few more replicates to losing some samples during a freezer failure. -To quantify Liberibacter development and associated tuber biochemical responses at different stages of curing, ramp-down,and 45°F storage in relation to the time of plant infestation In addition to theabove, Ialso completed the objective to generate a visual key for the growers. This key is very easy to follow and is expected to be utilized in by researchers and growers across the US.
Impacts
What was accomplished under these goals?
We continued to makesignificant progress in all of the proposed research objectives. All goals are expected to be achieved by the end of proposed timeline. Our findings have been communicated with our crop commissions and growers. This has generated a lot of support from our stakeholders for various ongoing programs. In addition we provided diagnostic services to our growers in response to outbreak of Barley Yellow Dwarf disease in Idaho in 2015 season. A move which was partly supported by Hatch funding. Below I provided a summary of our progress inindividual projects. 1-To quantify susceptibility and physiological responses of Idaho wheat cultivars, to bird cherry-oat aphidinfestations and Barley Yellow Dwarf Virus (BYDV) infection, at different plant developmental stages: -First year field data have been collected and analyzed. Second year data collection was completed successfully.We are analyzing collected samples from year two. Results of our first year data was analyzed and presented in meetings. Despite some variations no statistical difference existed among varieties and the addition of our second year data is expected to allow the detection of statistical differences. -We completed our greenhouse study and are currently conducting qPCR on collected samples. Failure in our -80 freezer resulted in some sample losses and we are hoping to be able to repeat a repeat a part of the experiment to regenerate some of the lost data. 2- To plot a distribution map and construct a visual identification key to the wireworm species present in south central andeastern Idaho. Key species included: Limonius californicus (known as sugar beet wireworm) Aeolus mellillus (Say) Hypnoidus bicolor (Eschscholtz) Limonius canus LeConte Limonius infuscatus Motschulsky (known as western field wireworm) Selatosomus aeripennis (Kirby) Selatosomus pruininus (Horn) (known as Great Basin wireworm) Hadromorphus glaucus (Germar) Overall more than 60% of the collected samples belonged to the L. californicus species group. -The objective of generating a visual key for Idaho species was completed. This detailed document is currently available to stakeholder and researchers. We also produced a one page visual key for distribution among all grower in different venues. -We collecteddata on soil characteristics (texture, organic matter, pH, density) that may help to predict speciesdistribution pattern across Idaho. This will continue for the second year. So far our analyses indicated a strong negative relationship between soil bulk density and wireworm pressure. -We also completed a greenhouse study to evaluate potential ecological variables that may impact wireworm damage. While seeding depthdid not appear to be a significant factor, soil type and crop type showed some impact. Barley appeared to be more resistant to damage than wheat. Moreover post emergence damage was primarily associated with soil with lower bulk density. 4-To quantify Liberibacter development and associated tuber biochemical responses at different stages of curing, ramp-down,and 45°F storage in relation to the time of plant infestation and the number of bacteriliferous psyllids -First year data was presented at two different meetings and published as a proceedings article. We have completed our second year data collection and are currently analyzing our two-year data. We are planning to have this work submitted for journal publication by the next reporting period. Results were novel aswe showed, for the first time, that Liberibacter continues to interact with harvested tubersduring different stages of storage. Tubers collected from plants which were infected only days before harvest all testednegative for the bacteria at harvest. A considerable percentage of the same tubers, however, tested positive followingstorage. This indicated that the pathogen would arrive to the tuber tissue shortly after inoculation by the vectore, they remainat undetectable levels. Exposure to relatively higher temperatures (e.g. 55F) post-harvest may facilitate pathogen
Publications
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Novy, R., Schneider, B., Rashidi, M., Rashed, A., Rondon S. Screening of potato breeding clones for response to zebra chip (ZC) disease and observations on Lso titer and ZC symptom expression in foliage and tuber. Potato association of America Annual Meeting.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Rashed, A. Workneh, F., Paetzold, L., Olsen, N., Rush, C.M. The effects of vector density and the time of infections on Zebra Chip disease development in the United States. World Potato Congress, China, July 2015.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
M. Rashidi, X. Liang, C. W. Rogers, E. Wenninger, N. Olsen, A. Karasev, P. Wharton, A. Rashed.Zebra chip disease development in potato in relation to vector density and the time of infection pre- and post-harvest . Entomological Society of America. Minneapolis, MN, November, 2015.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Zebra Chip Complex: Vector density, time of infection, and disease development. Program symposium: Potato psyllid and zebra chip disease: a threat to the Pacific Northwest potato industry. Pacific Branch Entomological Society of America, Coeur dAlene, ID, April 2015.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Host plant-virus-vector interactions and barley yellow dwarf disease management. Program symposium: Arthropod Interactions with Plants and Pathogens. Pacific Branch Entomological Society of America, Coeur dAlene, ID, April 2015.
- Type:
Other
Status:
Published
Year Published:
2015
Citation:
Rashed, A., Wallis, C.M., Paetzold, L., Woodell, L., Olsen, N., Workneh, F., Rashidi, M. 2015. Candidatus Liberibacter solanacearum (Lso) development in Russet Norkotah under commercial storage conditions. In: Workneh, F. and Rush, C. M. (eds). Proceedings of the 14th 2014 Annual SCRI Zebra Chip Reporting Session. Fredric Printing, Aurora, CO.
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Workneh, F., Paetzold, L., Rashed, A., Rush, C.M. 2015. Population dynamics of released potato psyllids and their Bacteriliferous status in relation to disease incidence in caged field plots. Plant Disease, in press
- Type:
Other
Status:
Published
Year Published:
2015
Citation:
Rashed, A., Etzler, F., Rogers, C.W., Marshall, J.M. 2015. Wireworms in Idaho Cereals: A Guide to Monitor Numbers and Identify Predominant Species in the Intermountain Region. Bulletin 898.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2015
Citation:
Rush, C.M., Workneh, F., Rashed, A. 2015. Significance and epidemiological aspects of late-season infections in the management of potato zebra chip. Phytopathology, in press.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Rogers, C.W., J. Marshall, G. Hu, C. Evans, A. Rashed, and S. Pristupa. Cultivar, Nitrogen Rate and Application Timing Effects on Irrigated Winter Malt Barley Nitrogen Uptake. ASA-CSSA-SSSA Annual Meeting, Minneapolis, MN, November 2015.
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Progress 07/01/14 to 09/30/14
Outputs
Target Audience: Preliminary results have been presented to: -Research scientists in the fields of potato production, potato disease, vectore-borne plant pathogens, and cereal pests -Graduate and undergraduate students attending the Annual Zebra Chip Reporting Session, and Entomological Society of America Annual Meeting -Potato growers in Idaho (e.g. through various extension venues including field days, and Idaho Potato School) -Cereal growers in Eastern Idaho (e.g. workshops, cereal schools, field days, extension publication) Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? -A post doctoral student has been recruited, is beingtrained on various aspect of experimental design, pathogen quantification, data analysis, and data presentation. She was provided with the opportunity to be trained in other laboratories for various molecular techniques involving Liberibacter and BYDV. -The post doctoral fellow also attended two international meeting, which provided her with the opportunity to interact with wide range of researchers. -A PhD student has been recruited to our program and she is working on zebra chip disease project. -I have established collaborations with researchers from different institutions. -More than 25 growers in central and eastern Idaho have expressed interest in collaborations on wireworm project- We are excited about the number of growers involved and this would be a significant help in collecting data on wireworm distribution. How have the results been disseminated to communities of interest? Our results have been communicated through conference meetings, symposiums, field days, Cereal and potato schools, and pest reporting sessions. As mentioned earlier our audiences include growers, industry, researchers, and students attending annual conferences. What do you plan to do during the next reporting period to accomplish the goals? We have started data collection for all of the 4 proposed projects. We will contiue our data collection in the second year. I expect to have results finalized for the following 2 goals: -To quantify susceptibility and physiological responses of Idaho wheat cultivars, toRhopalosiphum padiinfestations and Barley Yellow Dwarf Virus infection, at different plant developmental stages -To quantify Liberibacter development and associated tuber biochemical responses at different stages of curing, ramp-down, and 45°F storage in relation to the time of plant infestation and the number of bacteriliferous psyllids In addition: I will also have a complete year-around data on ecological factors affecting fluctuations in wireworm numbers and species distribution (spatial and temporal) (objectives 2 and 3). I have identified growers who are interested to help us evaluate practices that may reduce wireworm pressure. In 2015, I will work with our local growers to establish the effectiveness of cultural/physical approaches in managing wireworm damage.
Impacts
What was accomplished under these goals?
We have made significant progress in all of the proposed research objectives. All goals are expected to be achieved by the end of proposed timeline. 1-To quantify susceptibility and physiological responses of Idaho wheat cultivars, toRhopalosiphum padiinfestations and Barley Yellow Dwarf Virus (BYDV) infection, at different plant developmental stages: -First year field data have been collected and analyzed. Data included choice experiments that evaluated Rh. padi preference for four commonly used wheat and barley varieties in eastern Idaho. Using field cage experiments we quantified disease the probability of BYDV incedence in the 4 winter wheat cultivars. Second year exeriment was planted in October and results would be available by the next reporting period. -First year greenhouse experiments have been completed. Plants infected with BYDV were sampled at different growth stages post-infection. All qPCR analyses of the collected tissues are completed and currently being analyzed statistically. Biochemical analyses are currently ongoing; all samples were sent to our collaborator in USDA-ARS, Parlier, CA. Second replicate of this study is currently ongoing. Data of the second replicate would be ready by the next reporting period. 2-To plot a distribution map and construct a visual identification key to the wireworm species present in south central and eastern Idaho -First year data collection is completed. we have reported 5 species of wireworms active in cereal fields in Idaho. The five species are: Limonius californicus, Hadromorphus glaucus,Aeolus mellillus,Solatosomus aeripennis, andAgriotes sp..L. californicus was the most common species (77.7% of the collected samples). Based on this finding our ecological studies will now be focused on this species of wireworms. Our preliminary data also indicates that L. californicus starts its activity when maximum air temperatures reach 55F. Therefore their presence may not be detectable before planting in some locations on higher elevations; a conclusion supported by our first year data. -A visual identification key to wireworm species in Eastern Idaho has been developed. We are currently working on an extension publication this visual will have be available to the growers. 3- To quantify ecological parameters of the most prevalent wireworm species and to establish species-specific integrated management approaches based on key behavioral/ecological traits -We are currently collecting data on soil characteristics (texture, organic matter, pH, density) that may help to predict species distribution pattern accross Idaho. -Meteorological have been collected throughout the year for each sampling periods (traps were replaced every 2-3 weeks). A comprehensive dataset on wireworm activity will be available by the next reporting period. 4-To quantify Liberibacter development and associated tuber biochemical responses at different stages of curing, ramp-down, and 45°F storage in relation to the time of plant infestation and the number of bacteriliferous psyllids -First year data collection is analyzed and the results will be available in a proceeding article, within the next month. Second year data have been collected and we are in the process of analyzing data from this two-year study for publication in a refereed journal. In sum, using quantitative PCR, we have shown, for the first time, that Liberibacter continues to interact with harvested tubers during different stages of storage. Tubers collected from plants which were infected only days before harvest all tested negative for the bacteria at harvest. A considerable percentage of the same tubers, however, tested positive following storage. This indicated that the pathogen would arrive to the tuber tissue shortly after inoculation by the vectore, they remain at undetectable levels. Exposure to relatively higher temperatures (e.g. 55F) post-harvest may facilitate pathogen development.
Publications
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Rashed, A, Wallis, C.M., Paetzold, L., Woodell, L., Olsen, N., Workneh, F., Rashidi, M., Wenninger, E.J., and Rush, C.M. Candidatus Liberibacter solanacearum development in Russet Norkotah under commercial storage conditions, In: Workneh, F. and Rush, C. M. (eds). Proceedings of the 14th 2013 Annual SCRI Zebra Chip Reporting Session. Fredric Printing, Aurora, CO.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Wallis, C. M., Rashed, A., Workneh, F., Paetzold, L., and Rush, C. M.Effects of Candidatus Liberibacter solanacearum infections on the physiology of tubers at different storage temperatures. Proceedings of the 14th 2013 Annual SCRI Zebra Chip Reporting Session. Fredric Printing, Aurora, CO.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2015
Citation:
Rashidi, M., Marshall, J.,�Bosque-P�rez, N., and�Rashed, A. Barley Yellow Dwarf Virus complex: Comparing vector-plant-pathogen interaction in winter wheat varieties. Poster, Entomological Society of America, Portland, OR.
- Type:
Other
Status:
Published
Year Published:
2014
Citation:
Marshall, J. and Rashed, A. 2014. Barley Yellow Dwarf Virus In Idaho Cereal Crops, J. M. Current Information Series (CIS) 1210, p. 4.
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