Source: COLORADO STATE UNIVERSITY submitted to NRP
NATIONAL ANIMAL GENOME RESEARCH PROGRAM
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1002276
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
NRSP-8
Project Start Date
Jan 6, 2014
Project End Date
Sep 30, 2018
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
COLORADO STATE UNIVERSITY
(N/A)
FORT COLLINS,CO 80523
Performing Department
Animal Science
Non Technical Summary
This project will identify what changes in the mare's uterus to allow her to recognize that a viable embryo is present.
Animal Health Component
40%
Research Effort Categories
Basic
50%
Applied
40%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
30538101020100%
Knowledge Area
305 - Animal Physiological Processes;

Subject Of Investigation
3810 - Horses, ponies, and mules;

Field Of Science
1020 - Physiology;
Goals / Objectives
Advance the status of reference genomes for all species, including basic annotation of worldwide genetic variation, by broad sequencing among different lines and breeds of animals. Develop strategies to identify and exploit genes and allelic variation that contribute to economically relevant phenotypes and traits, in part through improving functional annotation of the genomes of our species. Facilitate analysis, curation, storage, distribution and application of the enormous datasets now being generated by next-generation sequencing and related "omics" technologies with regard to animal species of agricultural interest.
Project Methods
Endometrial samples from pregnant and non-mated mares will be compared to identify changes in gene product associated with pregnancy status.

Progress 01/06/14 to 09/30/18

Outputs
Target Audience: The membership of NRSP-8 includes researchers with an interest in animal genomes, and as such, they constitute the primary group benefiting directly from its activities. However, the beneficiaries are much broader than the participants. Obviously, genotype (the genome) and its relationship to biological function (the phenome) is of critical consideration for many disciplines of animal science whose researchers benefit from NRSP-8 supported resources and knowledge. International scientists are another important stakeholder group (Figure 1). The global animal research community, draws on the resources developed by NRSP-8, and the beneficiaries are the global agri-animal industries including commercial breeders, producers, and other entities that rely on agricultural animals. Ultimately, the general public constitutes the ultimate stakeholder group for the efforts of NRSP-8. Thus, it is hard to overstate the impact that accelerated genetic improvement enabled by genomic information has had on decreasing the global environmental footprint of animal protein production. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Several graduate students have participated in these research projects. How have the results been disseminated to communities of interest?Findings have been shared by journal article publication and through presentation ant local, national and international meetings. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Through research, publications and participation in NSRP8 sponsored and other such professional meetings all 3 of the gor=als have been addressed. We have been particulalry successful in identifying transcriptomic and microRNA regulation in physiologic status (pregnancy), anddisease (insulin resiistance). Additionally, Dr Coleman has been very succesful in initial attempts to describe the microbiota of the healthy growing horse.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Cell-secreted vesicles containing microRNAs as regulators of gamete maturation. da Silveira JC, de �vila ACFCM, Garrett HL, Bruemmer JE, Winger QA, Bouma GJ. J Endocrinol. 2018 Jan;236(1):R15-R27. doi: 10.1530/JOE-17-0200. Epub 2017 Sep 4. Review. PMID: 28870888
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: SNP detection using RNA-sequences of candidate genes associated with puberty in cattle. Dias MM, C�novas A, Mantilla-Rojas C, Riley DG, Luna-Nevarez P, Coleman SJ, Speidel SE, Enns RM, Islas-Trejo A, Medrano JF, Moore SS, Fortes MR, Nguyen LT, Venus B, Diaz IS, Souza FR, Fonseca LF, Baldi F, Albuquerque LG, Thomas MG, Oliveira HN. Genet Mol Res. 2017 Mar 22;16(1). doi: 10.4238/gmr16019522
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Genetic parameters for fertility and production traits in Red Angus cattle. Boldt RJ, Speidel SE, Thomas MG, Enns RM. J Anim Sci. 2018 Sep 29;96(10):4100-4111. doi: 10.1093/jas/sky294. PMID: 30204881
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Cardiopulmonary remodeling in fattened beef cattle: a naturally occurring large animal model of obesity-associated pulmonary hypertension with left heart disease. Krafsur GM, Neary JM, Garry F, Holt T, Gould DH, Mason GL, Thomas MG, Enns RM, Tuder RM, Heaton MP, Brown RD, Stenmark KR.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Possible Role of MicroRNA in Equine Insulin Resistance: A Pilot Study Journal of Equine Veterinary Science, Volume 63, April 2018, Pages 74-79 Hugo da Costa Santos, Tanja Hess, Jason Bruemmer, Rebecca Splan


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:The membership of NRSP-8 includes researchers with an interest in animal genomes, and as such, they constitute the primary group benefiting directly from its activities. However, the beneficiaries are much broader than the participants. Obviously, genotype (the genome) and its relationship to biological function (the phenome) is of critical consideration for many disciplines of animal science whose researchers benefit from NRSP-8 supported resources and knowledge. International scientists are another important stakeholder group (Figure 1). The global animal research community, draws on the resources developed by NRSP-8, and the beneficiaries are the global agri-animal industries including commercial breeders, producers, and other entities that rely on agricultural animals. Ultimately, the general public constitutes the ultimate stakeholder group for the efforts of NRSP-8. Thus, it is hard to overstate the impact that accelerated genetic improvement enabled by genomic information has had on decreasing the global environmental footprint of animal protein production. Changes/Problems:No problems. Changes are focused onadding Dr Stephen Coleman and his laboratory to the project. His focus is on the microbiotoa of the growing horse with special emphasis on stress and health care. What opportunities for training and professional development has the project provided?This project has been critical in the training of undergraduate Honor's student, as well as MS and PhD candidates. How have the results been disseminated to communities of interest?The results have been published in scientific journals and presented internationally. THese conferences include the Internations=al Stallion Symposium, Equine Science Society Symposium and at the Equine Workshop as part of the Plant and Animal Genomics meeting. What do you plan to do during the next reporting period to accomplish the goals?Continue to mine data from the RNA seq experiments.

Impacts
What was accomplished under these goals? 1) Genome reference was enhanced as products of reproduction, including endometrium, placenta and developing sperm were included in the publications. 2) Current evaluation is focused on RNA seq generation of genes expressed in the endometrium with regard to pregnancy status. Additional research is focus on small non-coding RNA and their involvement in reproductive success. 3) Analysis and curation of our data will be possible as the data are soon to be shared with the FAANG project.

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Klohonatz KM, Cameron AD, Herenreder JR, da Silveira JC, Belk AD, Veeramachaneni DN, Bouma GJ, Bruemmer JE. 2016. Circulating miRNAs as potential alternative cell signaling associated with maternal recognition of pregnancy in the mare. Biol. Reprod. 95:124, 1-12.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Twenter HM, Belk AD, Klohonatz KM, Bass LD, Bouma JG, Bruemmer JE. 2017. An investigation into miRNAs in the equine epididymis as potential regulators of spermatozoal maturation. J Eq Vet Sci. 48:61-68
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Davis KA, Klohonatz KM, Bouma JG, Bruemmer JE. 2017. Androgen receptor in the term equine placenta. J. Equine Vet. Sci.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Loux SC, Scoggin KE, Bruemmer JE, Canisso IF, Troedsson MHT, Squires EL, Ball BA. 2017. Evaluation of circulating miRNAs during late pregnancy in the mare. PLoS ONE. 12(4): e0175045. https://doi.org/10.1371/journal.pone.0175045
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Da Silveira JC, de Avila ACFCM, Garrett HL, Bruemmer J, Winger Q, Bouma G. 2017. Cell-secreted vesicles containing microRNAs as regulators of gamete maturation. J Endocrinol. JOE-17-0200. Doi: 10.1530/LOE-17-0200.


Progress 10/01/15 to 09/30/16

Outputs
Target Audience:Scientific community and NRSP8 Multi-State Project participants Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?In both cases a graduate student and an undergraduate were involved in the experimental design, procedures, data analysis and publication. How have the results been disseminated to communities of interest?Both by publication in scientific journals and presentation at scientific meetings. Meetings included Plant and Animal Genomics, American Society of Animal Sciences, Rocky Mountain Reproduction Symposium and International Symposium for Stallion Reproduction. What do you plan to do during the next reporting period to accomplish the goals?We are currently evaluating both the transcriptome and miRNA targets associated with pregnancy status in the mare. We plan to present these data and the appropriate meetings and publish in appropriate journals.

Impacts
What was accomplished under these goals? Goal was the main focus. We were able to add specific information related to reproductive physiology status of the horse. In one case we identified genetic indicators associated with preganacy. In a second finding we were the first to report genetic regulators associated with sperm delivered material.

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Klohonatz K.M., Cameron A.D., Hergenreder J.R., da Silveira J.C., Belk A.D., Veeramachaneni D.N., Bouma G.J., Bruemmer J.E. 2016. Circulating miRNAs as Potential Alternative Cell Signaling Associated with Maternal Recognition of Pregnancy in the Mare. Biol Reprod. 2016 Dec;95(6):124
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Belk, A.D., Klohonatz, K.M., Bouma, G.J., Bruemmer, J.E. 2016. An investigation into miRNAs in the equine epididymis as a potential regulator of spermatozoal maturation. Journal of Equine Veterinary Science, 35(5), 386. http://dx.doi.org/10.1016/j.jevs.2015.03.016


Progress 10/01/14 to 09/30/15

Outputs
Target Audience:Scientific community and NRSP8 Multi-State Project participants Changes/Problems:No major changes or problems were encountered. What opportunities for training and professional development has the project provided?In both cases a graduate student and an undergraduate were involved in the experimental design, procedures, data analysis and publication. How have the results been disseminated to communities of interest?Both by publication in scientific journals and presentation at scientific meetings. Meetings included Plant and Animal Genomics, American Society of Animal Sciences, Rocky Mountain Reproduction Symposium and International Symposium for Stallion Reproduction. What do you plan to do during the next reporting period to accomplish the goals?We are currently evaluating both the transcriptome and miRNA targets associated with pregnancy status in the mare. We plan to present these data and the appropriate meetings and publish in appropriate journals.

Impacts
What was accomplished under these goals? Goal was the main focus. We were able to add specific information related to reproductive physiology status of the horse. In one case we identified genetic indicators associated with preganacy. In a second finding we were the first to report genetic regulators associated with sperm delivered material.

Publications


    Progress 01/06/14 to 09/30/14

    Outputs
    Target Audience: Scientific community and NSRP8 Multi-State Project participants Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Undergraduate Honors students, MS students and current PhD student training. ALl part of aforementioned data collection and generation. How have the results been disseminated to communities of interest? Minimally. Most data are still being analyzed. What do you plan to do during the next reporting period to accomplish the goals? Analyze data collected. Publish results. Seek additional funding to continue work.

    Impacts
    What was accomplished under these goals? Tissue was collected. Transcriptome data generated for endometrium associated with pregnancy status. Data generated via RNAseq of small ncRNA in circulation and culture media associated with pregnancy status.

    Publications