Source: UNIVERSITY OF KENTUCKY submitted to NRP
GENETIC CONTROL OF POD SHATTERING IN SOYBEANS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1002201
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jan 1, 2014
Project End Date
Apr 11, 2017
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF KENTUCKY
500 S LIMESTONE 109 KINKEAD HALL
LEXINGTON,KY 40526-0001
Performing Department
Plant and Soil Sciences
Non Technical Summary
Legumes represent the second most important family of crop plants, accounting for ~27% of the world's crop production. While some legumes, such as alfalfa and clovers, are grown as forages, most crop legumes are grown for harvesting their nutritious seeds. The legume seeds are contained in the pod, which is composed of a single seed-bearing carpel that, when matures, splits open along two seams, a process called pod dehiscence or pod shattering. Pod shattering before harvest is a serious problem that causes yield loss of grain legumes. Furthermore, the dominant shattering trait of the wild progenitors is also a limiting factor for efficient introgression of value-added traits into elite breeding lines. The overall goal of this proposal is to elucidate the genetic mechanisms underlying pod shattering in soybeans. Knowledge of the genetic mechanisms underlying pod shattering will facilitate the breeding of shattering-resistant varieties, expedite the introgression of agronomically favorable traits from wild species to elite breeding lines, and enrich our understanding of the evolution of seed dispersal and crop domestication in diverse crop species.
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20118201080100%
Knowledge Area
201 - Plant Genome, Genetics, and Genetic Mechanisms;

Subject Of Investigation
1820 - Soybean;

Field Of Science
1080 - Genetics;
Goals / Objectives
Legumes represent the second most important family of crop plants, accounting for ~27% of the world's crop production. While some legumes, such as alfalfa and clovers, are grown as forages, most crop legumes are grown for harvesting their nutritious seeds. The legume seeds are contained in the pod, which is composed of a single seed-bearing carpel that, when matures, splits open along two seams, a process called pod dehiscence or pod shattering. Pod shattering before harvest is a serious problem that causes yield loss of grain legumes. Furthermore, the dominant shattering trait of the wild progenitors is also a limiting factor for efficient introgression of value-added traits into elite breeding lines. The overall goal of this proposal is to elucidate the genetic mechanisms underlying pod shattering in soybeans. Knowledge of the genetic mechanisms underlying pod shattering will facilitate the breeding of shattering-resistant varieties, expedite the introgression of agronomically favorable traits from wild species to elite breeding lines, and enrich our understanding of the evolution of seed dispersal and crop domestication in diverse crop species. The specific objectives of this proposal are: 1) Map-based cloning of a major quantitative trait locus (GmSH1) that regulates pod shattering in soybeans; 2) Molecular and functional characterization of GmSH1; and 3) Molecular diversity analysis at, and in the vicinity of, the GmSH1locus.
Project Methods
Genetic, genomic, and molecular approaches.

Progress 01/01/14 to 04/11/17

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?We have published our results and gave several talks in national and international conferences. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? We have developed breeder-friendly molecular markers for maker-assisted selection of shattering-resistant genotypes in soybeans. However, we did not proceed to clone the gene because a Japanese research group conducted the same project and cloned the QTL. We continued to work on nitrogen-fixing root nodule symbioses in legumes. We have successfully cloned a couple of genes that control nodulation specificity in soybeans and Medicago. This knowledge will help to improve nitrogen fixation capacity in crop legumes.

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Fan Y, Liu J, Lyu S, Wang Q, Yang S, Zhu H (2017) The soybean Rfg1 gene restricts nodulation by Sinorhizobium fredii USDA193. Frontiers in Plant Science doi: 10.3389/fpls.2017.01548
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Wang Q, Yang S, Liu J, Terecskei K, �brah�m E, Gomb�r A, Domonkos �, Sz?cs A, K�rm�czi P, Wang T, Fodor L, Mao L, Fei Z, Kondorosi �, Kal� P, Kereszt A, Zhu H (2017) Host-secreted antimicrobial peptide enforces symbiotic selectivity in Medicago truncatula. Proceedings of the National Academy of Sciences USA 114: 6854


Progress 10/01/14 to 09/30/15

Outputs
Target Audience: Nothing Reported Changes/Problems:AJapanese research group conducted the same project and cloned the QTL. We will have to terminate this project and propose a new Hatch project in the coming fisical year. What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? We have devloped breeder-friendly molecular markers for maker-assited selection of shattering-resistant genotypes in soybean.

Publications


    Progress 01/01/14 to 09/30/14

    Outputs
    Target Audience: Nothing Reported Changes/Problems: A Japanese group has cloned the target gene, so we will have to change our research objectives. We will focus on studying the domestication of the pod shattering gene. What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Molecular diversity analysis in the vicinity of the GmSH1locus.

    Impacts
    What was accomplished under these goals? We have developed robust molecular markers for selection of the pod shattering resistant trait in soybeans.

    Publications