Progress 10/01/14 to 09/30/15
Outputs
Target Audience:The work completed during the review period can be applied by primary breeders of poultry. The PD is scheduled to visit Cobb-Vantress during the week of September 5, 2016. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?Yes, via publication and an imminent site visit to the largest primary breeder of chickens in the USA. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
The work accomplished is ancillary to the primary objectives of the project, namely, in vitro storage of fowl sperm.
Publications
- Type:
Journal Articles
Status:
Awaiting Publication
Year Published:
2016
Citation:
D.P. Froman, Deduction of a calcium circuit affecting rooster sperm in vitro, Journal of Animal Science
|
Progress 01/03/14 to 09/30/14
Outputs
Target Audience: Attended the annual meeting of NC1170 Advanced Technologies for the Genetic Improvement of Poultry. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? The JAS manuscript referred to above served as a context for a PhD student at the University of Arkansas to participate as a collaborator. Specifically, the corresponding author (D. P. Froman) shipped DNA samples in assay blocks collected according to Bailes et al. (2007), An inexpensive, simple protocol for DNA isolation from blood for high-throughput genotyping by polymerase chain reaction or restriction endonuclease digestion, Poultry Science 86:102-106. Dr. Douglas Rhoads was the senior author on this techniques paper and is my NC1170 collaborator at the University of Arkansas. Sohita Ojha is a PhD student in Dr. Rhoads' laboratory. Ms. Ojha prepared DNA for the GWAS performed with 60k SNP chips at DNALandmarks in Canada. Ms. Ojha also helped with data analysis. How have the results been disseminated to communities of interest? Data were presented at the annual meeting of NC1170 Advanced Technologies for the Genetic Improvement of Poultry. What do you plan to do during the next reporting period to accomplish the goals? Phenotype roosters within a random bred F3 generation and perform GWAS on those roosters whose phenotype is less than or greater than a standard deviation from the population mean.
Impacts
What was accomplished under these goals?
Phenotyped F2 roosters from a reciprocal cross (low and high sperm mobility lines). GWAS performed on those roosters whose phenotype was less than or greater than one standard deviation from the population mean. A manuscript entitled "Identification of quantitative trait loci associated with low sperm mobility in the chicken" was submitted to the Journal of Animal Science. One reviewer deemed the work a "good contribution" to the field. The second reviewer . . . how shall I say this gently . . . ripped the authors a new one. Therefore, a decision was made to produce an F3 generation of random bred birds and perform a second GWAS
Publications
|
|