Source: NORTH CAROLINA STATE UNIV submitted to NRP
IDENTIFICATION, CHARACTERIZATION AND EFFECTIVE DELIVERY OF HEALTH-PROTECTIVE PHYTOACTIVE COMPOUNDS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1001550
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Nov 19, 2013
Project End Date
Sep 30, 2018
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
NORTH CAROLINA STATE UNIV
(N/A)
RALEIGH,NC 27695
Performing Department
Food, Bioprocessing, and Nutrition Sciences
Non Technical Summary
General functional food health benefits have been ascribed to various whole fruits or vegetables, from berries to broccoli, but few research probes have successfully identified and characterized the health-relevant phytoactive compounds that accumulate within those fruits or vegetables, nor determined their interactive effects. This project will strive to cleanly and nonintrusively isolate and unravel some of the potentiating interactions that condition the bioactivity of phytochemicals delivered together in a food matrix or an overall diet. It will explore ways of complexing together food components (phytoactive polyphenolic chemicals and edible proteins) to increase the functionality, and potency/bioavailability for consumers, and, to moderate potential allergenicity.
Animal Health Component
20%
Research Effort Categories
Basic
70%
Applied
20%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
50211991010100%
Knowledge Area
502 - New and Improved Food Products;

Subject Of Investigation
1199 - Deciduous and small fruits, general/other;

Field Of Science
1010 - Nutrition and metabolism;
Goals / Objectives
This project will endeavor to refine and optimize effective strategies for isolation and characterization of phytoactive compounds, individually or in potentiating mixtures, to allow validation of their health relevance. This project will further identify and quantify the influences of delivery vehicles on the structural integrity, efficacy, and bioavailability of phytoactive compounds which contribute to human health protection.
Project Methods
Phytochemical & Metabolomic Analysis. Our laboratory will refine and validate a streamlined multi-tiered approach to phytochemical analysis in foods or plant-based food products, and in tissues/fluids post ingestion and metabolism. The strategy includes counter current chromatography and centrifugal partition chromatography (CCC), thin layer chromatography (TLC), high performance liquid chromatography (HPLC), liquid chromatography-mass spectroscopy (LC-MS), and (when mixtures are highly purified) nuclear magnetic resonance spectrometry (NMR), in tandem with a suite of bioassays to assess activity. Metabolomics is used to evaluate effects of supplementation on chronic inflammation, oxidative stress, arterial stiffness, immunomodulation, and/or the presence of characteristic polyphenolic signatures in blood after colonic microfloral metabolism of parent phytoactive compounds. Experiments will determine how strategic complexes from two or more divergent food sources (protein source and phytoactive source) can be engineered, merging benefits from both, to produce shelf stable, efficacious, and convenient, health-protective food products. Tandem investigations will predict the bioefficacy and stability of the chimeric ingredients created with different whey proteins, and gauge the enhanced functionality (modified reactivity) of protein-polyphenol particles in traditional food structural elements (gels, sols, and foams) to advance the stability and hedonic characteristics of foods. A third related probe under this project umbrella is based on the hypothesis that when phytoactive polyphenolic compounds are deliberately complexed with peanut proteins (as in roasted peanut flour), the allergenic epitopes are changed/masked and rendered hypoallergenic, enabling suppression of peanut-specific T cell responses. IgE binding properties of the complexed peanut protein-polyphenolic chimeric ingredient are dependent on the species and concentration of polyphenolic phytochemical bound to peanut epitopes, and specifically proanthocyanidins (fruit-derived tannins) will be most effective in binding peanut proteins and disrupting IgE binding. We predict that the prepared, bound matrices (peanut protein-polyphenolic matrices) will synergistically mediate peanut allergy because peanut proteins will be hypoallergenic, as epitopes will be masked/modified by bound phytoactive polyphenols, and the phytoactives will serve as immune adjuvants by suppressing Th2 cytokine secretion and thus IgE production specific to peanut.

Progress 11/19/13 to 09/30/18

Outputs
Target Audience:Scientific audiences (National & International Science Forums, Conferences) Lay audiences (local and regional committees) Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Two postdoctoral research associates have been assigned to this project One undergraduate international intern (Serena Wang, University of Surrey) accepted aspects of this project for her special international training experience. How have the results been disseminated to communities of interest?Multiple seminars and symposia were presented. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Prototype bar formulations were developed using 6 alternative polyphenol resources (pomace or juices from muscadine, cranberry, and blueberry) and 6 alternative protein sources. Preliminary bioactivity assessments were performed in vitro.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: The Future of Healthcare & Nutrition  A Wholistic Intersection. Clinical care, nutritional therapies, sustainable agricultures. [Standard Process Grand Opening/Ribbon Cutting and Global Health Symposium] (04/2018) Discovering the therapeutic properties of whole foods. Kannapolis, NC
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Chemisty Department and Food Science Department Seminars, University of Surrey (5/23/2018) Discovery & Delivery of Health-Protective Phytoactive Chemicals. Guildford, United Kingdom
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: XXIX International Conference on Polyphenols. Human and Animal Nutrition & Health. (07/2018) Plenary Speaker. Answering the Call of the Wild: Polyphenols in Traditional Therapeutic Practice. Madison, WI.


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:The Target Audience(s) for this project include: - industry partners (Reoxcyn, General Mills, Latium, Standard Process) who are interested in the potential delivery mechanisms for getting phytoactive natural constituents into functonal foods and topical applications - community and members of the general public who are informed of this research through popular media/press releases as well as public seminars - scientific collaborators at University of Alaska Fairbanks, NC A&T University, Appalachian State University, Purdue University and New Mexico State University - graduate students and undergraduate interns who are trained in some of the phytochemical analytical techniques and in the characterization of phytoactive compound metabolites in human fluids Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The bioavailability assessment tactics as well as the phenotypic characterization methods were used in part to help train summer interns in the Plant Pathways Elucidation Project, a multiyear public-private program resident here on the North Carolina Research Campus How have the results been disseminated to communities of interest?Seminars to industry consortia (Dole Nutrition Institute, General Mills, Standard Process), professional venues (e.g. CED Symposium, Salisbury Business Leaders Consortium, Global Shea Alliance, CALS Advancement team, Food Industry Bloggers) and international conferences (ICoFF; International Breast Cancer Prevention Symposium, Institute of Arctic Biology, ASHS, ICPH, etcetera) What do you plan to do during the next reporting period to accomplish the goals?Experimental protocols to quantify phytoactive constituent bioaccessibility and stability and to assess sensory characteristics are scheduled as part of the ongoing FFAR project (still in its first year) on the protein-polyphenol aggregate ingredients described in the Hatch proposal

Impacts
What was accomplished under these goals? Based on previous work with delivery of complexed phytoactive compounds in a protein matrix, the LilaLab validated the enhanced stability of the complex, and used this information to help secure a $2M award for a 4 year project with the Foundation for Food and Agriculture Research.

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Van de Velde, Franco, Mary H. Grace, Debora Esposito, Maria Elida Piovani, and Mary Ann Lila. 2016. Quantitative comparison of phytochemical profile, antioxidant, and anti-inflammatory properties of blackberry fruits adapted to Argentina. Journal of Food Composition and Analysis 47:82-91.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Lila, Mary Ann, Britt Burton-Freeman, Mary H. Grace and Willy Kalt. 2016. Unraveling anthocyanin bioavailability for human health. Annual Reviews Food Science & Technology 7:17.117.19 doi:10.1146/annurev-food-041715-033346 PMID: 26772410
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Schneider, Margaret, Debora Esposito, Mary Ann Lila and E. Allen Foegeding. 2016. Formation of whey protein-polyphenol meso-structures as a natural means of creating functional particles. Food & Function 7:1229-1318. DOI: 10.1039/C5FO01499A PMID 26857696
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Collins, B. J. Hoffman, K.Martinez, M. Grace, M.A. Lila, C. Cockrell, A.Nadimpalli, E. Chang, .C-C. Chuang, W. Zhong, J. Mackert; W. Shen,P. Cooney, R. Hopkins, M. McIntosh. 2016. A polyphenol-rich fraction obtained from table grapes decreases adiposity, insulin resistance, and markers of inflammation and impacts gut microbiota in high-fat fed mice. Journal of Nutritional Biochemistry 31:150-165. PMID: 27133434 PMCID: PMC4933298 doi:10.1016/j.jnutbio.2015.12.021
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Grace, Mary H., Debora Esposito, Michael A. Timmers, Jia Xiong, Gad G. Yousef, Slavko Kormarnytsky, Mary Ann Lila. 2016. Chemical composition, antioxidant and anti-inflammatory properties of pistachio hull extracts. Food Chemistry 210:85-95. PMID: 27211624 doi:10.1016/j.foodchem.2016.04.088
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Blumberg, Jeffrey B., Arpita Basu, Christian G. Krueger, Mary Ann Lila, Catherine C. Neto, Janet A. Novotny, Jess D. Reed, Ana Rodriguez-Mateos, and Cheryl D. Toner. 2016. The impact of cranberries on gut microbiota and cardiometabolic health: Proceedings of the Cranberry Health Research Conference 2015. Advances in Nutrition 7:759S-770S. PMID: 27422512. PMCID: PMC4942875 doi:10.3945/an.11601.2583
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Kellogg, J., D. Barry Croom, Nathalie J. Plundrich, Brittany Graf, Rebecca F. Taylor, Ilya Raskin, Mary Ann Lila. 2016. Engaging American Indian/Alaska Native (AI/AN) students with participatory bioexploration assays. NACTA Journal 60:42-50.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Grace, Mary H., Debora Esposito, Michael A. Timmers, Jia Xiong, Gad Yousef, Slavko Komarnytsky, Mary Ann Lila. 2016. In vitro lipolytic, antioxidant and anti-inflammatory activities of roasted pistachio kernel and skin consitituents. Food & Function 7:4285-4298. PMID: 27604963 DOI: 10.1039/C6FO00867D
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Overall, John, Sierra Bonney, Mickey Wilson, Arnold Beermann III, Mary H. Grace, Debora Esposito, Mary Ann Lila and Slavko Komarnytsky. 2017. Metabolic effects of berries with structurally diverse anthocyanins. International Journal of Molecular Sciences 18: 422. doi:10.3390/ijms18020422
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Grace, Mary H., Yin Qiang, Shengmin Sang and Mary Ann Lila. 2017. One-step isolation of carnosic acid and carnosol from rosemary by centrifugal partition chromatography. Journal of Separation Science 40:1057-1062. doi: 10.1002/jssc.201601063 PMID: 28008719
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Plundrich, N., Rishipal Bansode, Leonard Williams and Mary Ann Lila. 2017. Protein-bound Vaccinium fruit polyphenols decrease IgE binding to peanut allergens and RBL-2H3 mast cell degranulation in vitro. Food & Function 8:1611-1621. DOI: 10.1039/C7FO00249A
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Nieman, David, Sivapriya Ramamoorthy, Colin Kay, Courtney Goodman, Christopher Capps, Zach Shue, Nicole Heyl, Mary H. Grace and Mary Ann Lila. 2017. Influence of ingesting a flavonoid-rich supplement on the metabolome and concentration of urine phenolics in overweight/obese women. Journal of Proteome Research 16:2924-2935.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Lila, Mary Ann, Maggie Schneider, Amy Devlin, Nathalie Plundrich, Scott Laster and E. Allen Foegeding. 2017. Polyphenol-enriched berry extracts naturally modulate reactive proteins in model foods. Food & Function 8:4760-4767. DOI: 10.1039/C7FO00883J


Progress 10/01/15 to 09/30/16

Outputs
Target Audience:Peer Institutions and Professional Organizations: University of Wisconsin-Madison; University of Texas San Antonio; American Council for Medicinally-Active Plants; Wild Blueberry Association of North America; Womack Army Medical Center Media and Popular Press: WCNC-TV (Charlotte), Correio da Manha (Portuguese), Charlotte Business Journal, Food Technology, Fresh Plaza Italia, Eater, The New York Times Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Refereed journal publications Poster presentations Popular press coverage Invited presentations What do you plan to do during the next reporting period to accomplish the goals?Conduct a medium scale in vivo trial with neo-natal piglets to investigatet he ability of the aggregate particles to attenuate allergenic responses.

Impacts
What was accomplished under these goals? Accomplished scaled up protocols for protein-polyphenol aggregate particles and optimized process using microwave-assisted extraction. Partnerships forged with commercialization partners on the North Carolina Research Campus to open up new opportunities for the production of highly bioavailable protein-polyphenol aggregate particles as an ingredient or supplement.

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Xiong, Jia, Mary H. Grace, Debora Esposito, Fei Wang, and Mary Ann Lila. 2016. Phytochemical characterization and antiinflammaory properties of Acacia mearnsii leaves. Natural Product Communications 11(5):649-653.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Grace, Mary H., Debora Esposito, Michael A. Timmers, Jia Xiong, Gad G. Yousef, Slavko Kormarnytsky, Mary Ann Lila. 2016. Chemical composition, antioxidant and anti-inflammatory properties of pistachio hull extracts. Food Chemistry 210:85-95. doi:10.1016/j.foodchem.2016.04.088
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Schneider, Margaret, Debora Esposito, Mary Ann Lila and E. Allen Foegeding. 2016. Formation of whey protein-polyphenol meso-structures as a natural means of creating functional particles. Food & Function 7:1229-1318. DOI: 10.1039/C5FO01499A
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Kellogg, J., D. Barry Croom, Nathalie J. Plundrich, Brittany Graf, Rebecca F. Taylor, Ilya Raskin, Mary Ann Lila. 2016. Engaging American Indian/Alaska Native (AI/AN) students with participatory bioexploration assays. NACTA Journal 60:42-50.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Timmers, M., Jorge L. Guerrero-Medina, Debora Esposito, Mary H. Grace, Octavio Paredes-Lopez, Pedro A. Garc�a-Saucedo, and Mary Ann Lila. 2015. Characterization of phenolic compounds and antioxidant and antiinflammatory activities from mamuyo (Styrax ramirezii Greenm.) fruit. Journal of Agricultural and Food Chemistry 63: 10459-10465. http://dx.doi.org/10.1021/acs.jafc.5b04781
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Esposito, Deborah, Thanakorn Damsud, Mickey Wilson, Mary H. Grace, Renee Strauch, Xu Li, Mary Ann Lila, and Slavko Komarnytsky. 2015. Blackcurrant anthocyanins attenuate weight gain and improve glucose metabolism in diet-induced obese mice with intact, but not disrupted, gut microbiome. Journal of Agricultural and Food Chemistry 63: 6172-6180. DOI: 10.1021/acs.jafc.5b00963
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Collins, B. J. Hoffman, K.Martinez, M. Grace, M.A. Lila, C. Cockrell, A.Nadimpalli, E. Chang, .C-C. Chuang, W. Zhong, J. Mackert; W. Shen,P. Cooney, R. Hopkins, M. McIntosh. 2016. A polyphenol-rich fraction obtained from table grapes decreases adiposity, insulin resistance, and markers of inflammation and impacts gut microbiota in high-fat fed mice. Journal of Nutritional Biochemistry 31:150-165. doi:10.1016/j.jnutbio.2015.12.021


Progress 10/01/14 to 09/30/15

Outputs
Target Audience:media American Institute for Cancer Research - reporter Cathy Wolz. Antioxiddants and how they condition foods of tomorrow. 3/15/2015 Healthline News. Avoiding peanuts in childhood makes kids more likely to become allergic. reporter Kristen Fischer 4/23/2015 WTVI PBS Station, Charlotte NC, Reporters Jeff Rivenbark and Danielle Kosir. Prevention and management of diabetes with functional foods 3/31/15 Today's Dietician 16:(6) 32-32 Reporter Jasenka Piljac Zegarac. The allure of exotic berries. 6//15/2015 Alaska Native Dispatch Health propoerties of Alaska aquatic vegetation 6/15/2015 Scientific audiences: Experimental Biology Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Refereed journal publications Poster presentations Popular press coverage What do you plan to do during the next reporting period to accomplish the goals?Move allergenicity and food functionality work into animal models to validate results in vivo

Impacts
What was accomplished under these goals? Validated the hypoallergenic nature of protein-polyphenol aggregate particles and their utility for allergy immunotherapy, leading to awards of 1 internal and 2 external grants.

Publications

  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Guzman, I., M.H. Grace, G. G. Yousef, I. Raskin, and Mary Ann Lila. 2015. Novel strategies for capturing health-protective mango phytochemicals in shelf stable food matrices. International Journal of Food Sciences and Nutrition 66:175-185. (doi:10.3109/09637486.2014.979315)
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Kellogg, Joshua, Debora Esposito, Mary H. Grace, Slavko Komarnytsky and Mary Ann Lila. 2015. Alaskan seaweeds lower inflammation in RAW 264.7 macrophages and decrease lipid accumulation in 3T3-L1 adipocytes. Journal of Functional Foods 15:396-407. 10.1016/j.jff.2015.03.049
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: McOliver, Cynthia, Anne K. Camper, John T. Doyle , Margaret J. Eggers, Tim E. Ford, Mary Ann Lila, James Berner, Larry Campbell and Jamie Donatuto. 2015. Community-based research as a mechanism to reduce environmental health disparities in American Indian and Alaska Native communities. Special issue| Eliminating Health Disparities to Achieve Health Equity. International Journal of Environmental Research and Public Health 12:4076-4100. doi:10.3390/ijerph120404076
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Plundrich, Nathalie, Brittany White, Lisa L. Dean, Jack P. Davis, E. Allen Foegeding, and Mary Ann Lila. 2015. Stability and immunogenicity of hypoallergenic peanut protein-polyphenol complexes during in vitro digestion. Food & Function 6: 2145-2154. DOI: 10.1039/C5FO00162E
  • Type: Book Chapters Status: Published Year Published: 2014 Citation: Cuevas-Rodriguez, Edith-Oliva, Mary Ann Lila, and Octavio Paredes-Lopez. 2014. Antocianinas y proantocianidinas de zarzamoras silvestres mexicanas. Extraccion, caracterizacion, y bioactividad. PUBLICA. 167 pp. ISBN 978-3-639-5550.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Gupta, V., A.D. Estrada, I. Blakley, R. Reid, K. Patel, M. Meyer, S. Uggerhoj, A. Brown, M.A. Lila, A.E. Loraine. 2015. RNA-Seq analysis and annotation of a draft blueberry genome assembly identifies candidate genes involved in fruit ripening, biosynthesis of bioactive compounds, and stage-specific alternative splicing. GigaScience 4:5. doi:10.1186/s13742-015-0046-9
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Graf, Brittany, Diana M. Cheng, Deborah Esposito, Tara Shertel, Alexander Poulev, Nathalie Plundrich, David Itenberg, Nava Dayan, Mary Ann Lila, and Ilya Raskin. 2015. Compounds leached from quinoa seeds inhibit matrix metalloproteinase activity and intracellular reactive oxygen species. Int J Cosmetic Sci. 37:212-221.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Esposito, Deborah, Thanakorn Damsud, Mickey Wilson, Mary H. Grace, Renee Strauch, Xu Li, Mary Ann Lila, and Slavko Komarnytsky. 2015. Blackcurrant anthocyanins attenuate weight gain and improve glucose metabolism in diet-induced obese mice with intact, but not disrupted, gut microbiome. Journal of Agricultural and Food Chemistry 63: 6172-6180. DOI: 10.1021/acs.jafc.5b00963
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Grace, M.H., An Truong, Van-Den Truong, Ilya Raskin and Mary Ann Lila. 2015. Novel value-added uses for sweet potato juice and flour in polyphenol- and protein-enriched functional food ingredients. Food Science & Nutrition (3) 5:415-424. DOI: 10.1002/fsn3.234
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Turmagambetova, Aizhan S., Nadezhda Sergeevna Sokolova, Andrey Pavlinovich Bogoyavlenskiy, Vladimir Eleazarovich Berezin, Mary Ann Lila, Diana M. Cheng and Vyacheslav Dushenkov. 2015. New functionally-enhanced soy proteins as food ingredients with anti-viral activity. Virus Disease 26 (3):123132. DOI 10.1007/s13337-015-0268-6


Progress 11/19/13 to 09/30/14

Outputs
Target Audience:Target audiences include scientific colleagues (university and industry), and public sector. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Presentations at Experimental Biology and in publications What do you plan to do during the next reporting period to accomplish the goals?Validate phytochemical content and shelf stability of aggregate particles.

Impacts
What was accomplished under these goals? Development of shelf-stable protein-polyphenol aggregate particles for MRE under direction of US Army Natick.

Publications

  • Type: Book Chapters Status: Published Year Published: 2014 Citation: Lila, Mary Ann. 2014. Specialty crops and human health impacts. In: NABC Report 25: Biotechnology and North American Specialty Crops: Linking Research, Regulation, and Stakeholders. (Allan Eaglesham, ed.) NABC 25: 237-244. National Agricultural Biotechnology Council.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Joseph, Gili, Mina Faran, Ilya Raskin, Mary Ann Lila and Bertold Fridlender. 2015. Medicinal plants of Israel: A model approach to enable an efficient, extensive and comprehensive field survey. International Journal of Biodiversity Bioprospecting and Development 1:134. doi: 10.4172/ijbbd.1000134
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Ahmed, Maryam, Dru Hensen, Matthew Sanderson, David Nieman, Nicholas Gillitt, and Mary Ann Lila. 2014. The protective effects of a polyphenol-enriched protein powder on exercise-induced susceptibility to virus infection. Phytotherapy Research 28:1829-1836. DOI: 10.1002/ptr.5208
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Grace, M.H., G. G. Yousef, D. Esposito, I. Raskin, and Mary Ann Lila. 2014. Bioactive capacity, sensory properties, and nutritional analysis of a shelf stable protein-rich functional ingredient with concentrated fruit and vegetable phytoactives. Plant Foods for Human Nutrition 69:372-378. 10.1007/s11130-014-0444-7
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Yousef, G.G., M.H. Grace, Jorge L. Guerrero Medina . Scott Neff . Ivette Guzman . Allan F. Brown, I. Raskin, and Mary Ann Lila. 2014. Concentrating immunoprotective phytoactive compounds from fruits and vegetables into shelf-stable protein-rich ingredients. Plant Foods for Human Nutrition 69:317-324. 10.1007/s11130-014-0445-6
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Le V, Esposito D, Grace M, Ha D, Pham A, Bortolazzo A, Bevens Z., Kim J, Okuda R, Komarnytsky S, Lila MA, White B. 2014. Cytotoxic effects of ellagitannins isolated from walnuts in human cancer cells. Nutrition and Cancer 66:1304-1314 doi: 10.1080/01635581.2014.956246
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: *191. Kellogg, Joshua, Mary H. Grace, and Mary Ann Lila. 2014. Phlorotannins from Alaskan seaweed inhibit carbolytic enzyme activity. Marine Drugs 12:5277-5294.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: *190. Lila, Mary Ann. 2014. Capitalizing on the health benefits of berryfruit: Science versus the marketplace. Proc. Second International Symposium on Biotechnology of Fruit Species. (ed. SE Gardiner). Acta Horticulturae 1048123-128.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Arthur, ST, KA Zwetsloot, MM Lawrence, DC Nieman, MA Lila, MH Grace, R Howden, ID Cooley, JF Tkach, MD Keith, JL Demick, SE Blanton, RS Greiner, AM Bradley, ME Davenport, V Badmaev and RA Shanely. 2014. Ajuga turkestanica increases Notch and Wnt signaling in aged skeletal muscle. European Review for Medical and Pharmacological Sciences 18: 2584-2592.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Fredes, Carolina, Gad G. Yousef, Paz Robert, Mary H. Grace, Mary Ann Lila, Miguel G�mez, Marlene Gebauer, and Gloria Montenegro. 2014. Anthocyanin profiling of wild maqui berries (Aristotelia chilensis [Mol.] Stuntz) from different geographical regions in Chile. Journal of the Science of Food and Agriculture 94:2639-2648.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Reid, Robert, Cory R. Brouwer, Eric Jackson and Mary Ann Lila. 2014. A need for a transdisciplinary environment: The Plant Pathways Elucidation Project  P2EP. Trends in Plant Science 19:485-487.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Esposito, Debora, Amelia Chen, Mary H. Grace, Slavko Komarnytsky and Mary Ann Lila. 2014. Inhibitory effects of wild blueberry anthocyanins and other flavonoids on biomarkers of acute and chronic inflammation in vitro. Journal of Agricultural and Food Chemistry 62:7022-7028. http://dx.doi.org/10.1021/jf4051599
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Plundrich, Nathalie, Mike Kulis, Brittany L. White, Mary H. Grace, Rishu Guo, A. Wesley Burks, Jack P. Davis and Mary Ann Lila. 2014. Novel strategy to create hypoallergenic peanut protein-polyphenol edible matrices for oral immunotherapy. Journal of Agricultural and Food Chemistry 62:7010-7021. http://dx.doi.org/10.1021/jf405773b
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Waterman, Carrie, Diana M. Cheng, Patricio Rojas-Silva, Alexander Poulev, Julia Dreifus, Mary Ann Lila, and Ilya Raskin. 2014. Stable, water extractable isothiocyanates from Moringa oleifera leaves attenuate inflammation in vitro. Phytochemistry 103: 114-122. http://dx.doi.org/10.1016/j.phytochem.2014.03.028
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Graf, Brittany L., Alexander Poulev, Peter Kuhn, Mary Grace, Mary Ann Lila, and Ilya Raskin. 2014. Quinoa seeds leach phytoecdysteroids and other compounds with anti-diabetic properties. Food Chemistry 163:178-185. http://dx.doi.org/10.1016/j.foodchem.2014.04.088
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Grace, Mary H., Debora Esposito, Kriya Dunlap, Mary Ann Lila. 2014. Comparative analysis of phenolic content and profile, antioxidant capacity, and anti-inflammatory bioactivity in wild Alaskan and commercial Vaccinium berries. Journal of Agricultural and Food Chemistry 62:4007-4017. http://dx.doi.org/10.1021/jf403810y
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Grace, M.H., Charles Warlick, Scott Neff and Mary Ann Lila. 2014. Efficient preparative isolation and identification of walnut bioactive components using high-speed counter-current chromatography and LC-ESI-IT-TOF-MS. Food Chemistry 158: 229-238. http://authors.elsevier.com/sd/article/S0308814614003021
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Yousef, Gad G., Mary Ann Lila, Ivette Guzman, James Ballington, and Allan Brown. 2014. Impact of interspecific introgression on anthocyanin profiles of southern highbush blueberry. Journal of the American Society for Horticultural Science 139:99112.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Strathearn, Katherine, Gad G. Yousef, Mary H. Grace, Susan Roy, Mitali Tambe, Mario G. Ferruzzi, Qing-Li Wu, James E. Simon, Mary Ann Lila, and Jean-Christophe Rochet. 2014. Neuroprotective effects of anthocyanin- and proanthocyanidin-rich extracts in cellular models of Parkinsons disease. Brain Research 1555:60-77.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Rojas-Silva, Patricio, Rocky Graziose, Brian Vesely, Alexander Poulev, Flaubert Mbeunkui, Mary H. Grace, Dennis E. Kyle, Mary Ann Lila, Ilya Raskin. 2014. Leishmanicidal activity of a daucane sesquiterpene isolated from Eryngium foetidum. Pharmaceutical Biology 52:398-401. DOI: 10.3109/13880209.2013.837077
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Lila, Mary Ann, J. Kellogg, M.H. Grace, G. G. Yousef, T. B. Kraft, and R. B. Rogers. 2014. Stressed for success: how the berrys wild origins result in multifaceted health protections. Acta Horticulturae (ISHS) 1017:23-43. http://www.actahort.org/books/1017/1017_1.htm
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Grace, Mary, Gad Yousef, Sally J. Gustafson, Van-Den Truong, G. Craig Yencho, Mary Ann Lila. 2014. Phytochemical changes in phenolics, anthocyanins, ascorbic acid, and carotenoids associated with sweetpotato storage and impacts on bioactive properties. Food Chemistry 145: 717-724. http://dx.doi.org/10.1016/j.foodchem.2013.08.107.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Ribnicky, David M., Diana E. Roopchand, Andrew Oren, Mary Grace, Alexander Poulev, Mary Ann Lila, Robert Havenaar, Ilya Raskin. 2014. Effects of a high fat meal matrix and protein complexation on the bioaccessibility of blueberry anthocyanins using the TNO gastrointestinal model (TIM-1). Food Chemistry 142:349-357. http://dx.doi.org/10.1016/j.foodchem.2013.07.073