Source: UNIVERSITY OF ARKANSAS submitted to NRP
ENHANCING MICROBIAL FOOD SAFETY BY RISK ANALYSIS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1001053
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
S-1056
Project Start Date
Oct 1, 2013
Project End Date
Sep 30, 2018
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF ARKANSAS
(N/A)
FAYETTEVILLE,AR 72703
Performing Department
Food Science
Non Technical Summary
The overalllong-term goal of this multi-state project is the establishment of a multi-disciplinary network of scientists including those from the University of Arkansasthatengage incomprehensive and integrated risk-based research and outreach to improve the safety of food from farm to fork. Interested stakeholders, including food producers and/or processors, retailers and consumers, have identified the need for an approach that conducts applied research to determine the prevalence and ecology of foodborne pathogens (including antibiotic resistant bacteria) in fresh and processed foods coupling that to research aimed at establishing effective control methods to decrease pathogen contamination of foods. Several outreach objectives have also been developed in support of this project. These objectives include communication of risk-based management recommendations derived from the research aspects of this proposal to stakeholders as well as to those who interact with stakeholders. Communication strategies will be precisely tailored to the particular audience (processors, distributors, retailers, consumers). Message content will focus on risk-based strategies and microbial control opportunities deemed critical for each target audience to achieve the greatest strides in improving food safety in the U.S. Outreach to those who advise producers and consumers (e.g. educators, extension personnel) who are not part of the project will be achieved through ongoing symposia to disseminate key information concerning lessons learned during the course of this project.
Animal Health Component
25%
Research Effort Categories
Basic
50%
Applied
25%
Developmental
25%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
7125010110050%
7113999116050%
Knowledge Area
711 - Ensure Food Products Free of Harmful Chemicals, Including Residues from Agricultural and Other Sources; 712 - Protect Food from Contamination by Pathogenic Microorganisms, Parasites, and Naturally Occurring Toxins;

Subject Of Investigation
3999 - Animal research, general; 5010 - Food;

Field Of Science
1160 - Pathology; 1100 - Bacteriology;
Goals / Objectives
Risk Assessment: Assess food safety risks in agriculture systems Risk Management: Develop science-based interventions to prevent and mitigate food safety threats Risk Communication: Communicate food safety messages to stakeholders
Project Methods
Efforts willinclude activities that deliver science-based knowledge to people through workshops, seminars to industry and food production commodity groups and local scientific forums such as food safety focused conferences where original research can be presented in either oral or poster format.Evaluation:Effectiveness of scientific forums, workshops and conferences will be quantitiated by number of regitrants that attend as well as the demand and growth of continuing to hold these events on an annual basis by the respective stakeholders.

Progress 10/01/13 to 09/30/18

Outputs
Target Audience:Academic researchers at other universities; federal institutions (FDA and USDA); food safety industry, in general Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Numerous MS and PhD students have been trained under the scope of the project. How have the results been disseminated to communities of interest?Results have been dessiminated via publications, presentations, and student theses. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? (1) Risk Assessment: Assess food safety risks in agriculture systems We continue to investigate the impact of microbe-microbe interactions on the persistence of human enteric viruses under varying conditions. Our data show the virus association with bacteria does not impact virus stability under extreme pH conditions. (2) Risk Management: Develop science-based interventions to prevent and mitigate food safety threats. Additional work has also been completed in the evaluation of a batch wash ozone sanitation system (BWOSS) for washing fresh produce in retail settings. Graduate student data indicate that the BWOSS does not effectively inactivate/remove viruses from leaf lettuce and tomatoes compared to a water only wash. In addition, experiments with virus associated with bacteria indciate important differences in Gram type with respect to removal and inactivation of virsues on fresh produce. Further characterization of the BWOSS confirms that free chlorine and water temperature negatively impact the ability of ozone to inactivate microbes ona variety of fresh produce. This information will lead to more effective interventions. (3) Risk Communication: Communicate food safety messages to stakeholders. Numerous presentations were given to communicate research findings and relevant risks to food safety.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Jones SL, Ricke SC, Roper DK, Gibson KE. ⿿Swabbing the surface: Critical factors in environmental monitoring and a path towards standardization and improvement⿝ Critical Reviews in Food Science and Nutrition. doi: 10.1080/10408398.2018.1521369. 2018.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Yeargin T, Gibson KE. ⿿Key characteristics of foods with elevated risk for viral enteropathogen contamination⿝ (Invited review) Journal of Applied Microbiology. doi: 10.1111/jam.14113. 2018.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Riggio G, Wang Q, Kniel K, Gibson KE. ⿿Microgreens⿿a review of food safety considerations along the farm to fork continuum⿝ International Journal of Food Microbiology. 290: 76-85. 2019.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2018 Citation: Dawley C, Gibson KE. ⿿Virus-bacteria interactions: implications for prevention and control from environment to host⿝ (Invited review) Foodborne Pathogens and Disease. Accepted. 2018.
  • Type: Book Chapters Status: Awaiting Publication Year Published: 2018 Citation: Gibson KE, D⿿Souza DH, Hall AJ. 2018 ⿿Foodborne Viral Pathogens⿝ Accepted. In: Food Microbiology: Fundamentals and Frontiers, 5th edition. ASM Press.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Deng W, Gibson KE. 2018. Effect of Bacteria on Bleach Inactivation of Human Norovirus Surrogates on Stainless Steel Surfaces. The 2018 Annual Meeting of the International Association for Food Protection, July 8-11, Salt Lake City, UT.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Almeida G, Gibson KE. 2018. Association of Tulane Virus with Bacterial Cell Components in Suspension. The 2018 Annual Meeting of the International Association for Food Protection, July 8-11, Salt Lake City, UT.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Clark J, et al. 2018. Testing Alternative Methodologies for Quantifying and Understanding Food Safety Behavior Relationships Among Restaurant Food Handlers. The 2018 Annual Meeting of the International Association for Food Protection, July 8-11, Salt Lake City, UT.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Almeida G, Dawley C, Gibson KE. 2018. Evaluation of Batch Wash Ozone Sanitation System (BWOSS) for Reduction of Microorganisms on Fresh Produce. The 2018 Annual Meeting of the International Association for Food Protection, July 8-11, Salt Lake City, UT


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:Academic researchers at other universities; federal institutions (FDA and USDA); food safety industry, in general Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Numerous undergraduate, MS, and PhD students as well as 2 post-docs have been trained under the scope of the project. How have the results been disseminated to communities of interest?Results have been dessiminated via publications, presentations, and student theses. What do you plan to do during the next reporting period to accomplish the goals?Research in food safety at the University of Arkansas will continue to focus on evaluating control and prevention strategies of foodborne pathogens including human norovirus, Salmonella, and Campylobacter.

Impacts
What was accomplished under these goals? (1) Risk Assessment: Assess food safety risks in agriculture systems We have been investigatin the impact of microbe-microbe interactions on the persistence of human enteric viruses under varying conditions. Our data show that both human norovirus (hNoV) and its surrogates rapidly associate with whole cell bacteria within 10 min with complete virus association within one hour. However, it seems the association with bacteria decreases the persistence of viruses under exposure to elevated temperatures (>37°C). Meanwhile, hNoV surrogate inactivation with bleach on stainless steel surfaces is dependent on the bacteria - surrogate virus combination with Gram-positive bacteria decreasing persistence. (2) Risk Management: Develop science-based interventions to prevent and mitigate food safety threats. We have continued assessing prebiotics to control Salmonella in poultry. In addition, we are in the process of developing a Salmonella vector carrier vaccine for Campylobacter. Work has also been completed in the evaluation of a batch wash ozone sanitation system (BWOSS) for washing fresh produce in retail settings. The BWOSS was compared to a water only wash step. The data indicate that more than a 6-log reduction in Salmonella and Listeria on head lettuce can be achieved by 30 and 40 min, respectively, using the BWOSS. Meanwhile, nearly a 4-log reduction was shown for Escherichia coli after 40 min. The water only wash step consistently resulted in less than a 2-log reduction in all bacteria after 40 min washing. The data also show reduction in cross-contamination in the water and surfaces of the wash basin when ozone is present. (3) Risk Communication: Communicate food safety messages to stakeholders. Numerous presentations were given to communicate research findings and relevant risks to food safety.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Atchley, J.A., S.A. Kim, C. Knueven, L.R. Meyer, and S.C. Ricke. 2017. Assessment of sodium bisulfate as a potential antimicrobial against Salmonella Typhimurium on chicken breast meat. 9th Annual Arkansas Association Protection Educational Conference, Holiday Inn, Springdale, AR., Sept. 19-21.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Micciche, A.C., H. O. Pavlidis, and S.C. Ricke. 2017. A preliminary overview of commercialized probiotics for food animal application, research directions and future commercial trends. 9th Annual Arkansas Association Protection Educational Conference, Holiday Inn, Springdale, AR., Sept. 19-21.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Bodie, A., S.A. Kim, J. Atchley, C. Knueven, and S.C. Ricke. 2017. Potential antimicrobials for controlling Listeria monocytogenes in hotdogs. 9th Annual Arkansas Association Protection Educational Conference, Holiday Inn, Springdale, AR., Sept. 19-21.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Wages, J.A., S.A. Kim, S.H. Park, S.C. Ricke, E. Oxford, R.O. Apple, and B.J. Bench. 2017.The application of next generation sequencing to evaluate the microbial ecology in commercial poultry processing environments. 9th Annual Arkansas Association Protection Educational Conference, Holiday Inn, Springdale, AR., Sept. 19-21.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Micciche, A.C., P. M. Rubinelli, Z. Shi, and S. C. Ricke. 2017. Comparison of DNA Extraction Methods for qPCR Detection of Pathogenic Campylobacter strains. 9th Annual Arkansas Association Protection Educational Conference, Holiday Inn, Springdale, AR., Sept. 19-21.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Deng W, Gibson KE. Interactions of microorganisms within leafy green phyllospheres: Where do human noroviruses fit in? International Journal of Food Microbiology. 258: 28-37 2017.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Turnage NL, Gibson KE. Sampling methods for recovery of human enteric viruses from environmental surfaces Journal of Virological Methods. 248: 31-38. 2017.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Yu H, Gibson KE, Wright KG, Neal JA, Sirsat SA. Food safety and food quality perceptions of farmers market consumers in the United States Food Control. 79: 266-271. 2017.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: International Association for Food Protection, Tampa,FL, July 2017 Poster Presentation: Efficacy of Hypochlorite Bleach Treatment on Different Human Norovirus Genotypes Authors: Flor Maes, Genesis Chavez Reyes, Giselle Almeida, Kristen E. Gibson
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Conover DM, Gibson KE. Impact of soap typefoaming vs. gel-basedon handwashing time Food Control. 73: 878-882. 2017.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: International Association for Food Protection, Tampa,FL, July 2017 Poster Presentation: Thermal Stability of Viruses in Coculture with Enterobacter cloacae Authors: Kristen E. Gibson and Giselle Almeida
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: International Association for Food Protection, Tampa,FL, July 2017 Poster Presentation: Rapid Association of Enteric Viruses with Whole Cell Bacteria in Suspension Authors: Giselle Almeida and Kristen E. Gibson
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: International Association for Food Protection, Tampa,FL, July 2017 Poster Presentation: Impact of Moisture Content and Temperature during Rice Storage on Levels of Mold and Aerobic Bacteria over Time Authors: Kristen E. Gibson, Giselle Almeida, Wenjun Deng, Bhagwati Prakash, Shweta Kumari, Terry Siebenmorgen
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: International Association for Food Protection, Tampa,FL, July 2017 Poster Presentation: Optimization of Virus Recovery from Non-porous Surfaces with Application in Environmental Persistence Studies Authors: Nicole Turnage and Kristen E. Gibson
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Rubinelli, P., S. Roto, S.A. Kim, Si H. Park, H.O. Pavlidis, D. McIntyre, and S.C. Ricke. 2016. Reduction of Salmonella Typhimurium by fermentation metabolites of Diamond V Original XPC in an in vitro anaerobic mixed culture. Front. Vet. Sci. 3:83. doi: 10.3389/fvets.2016.00083.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Baker, C.A., P.M. Rubinelli, S. H. Park, F.Carbonero, and S.C. Ricke. 2016. Shiga toxin-producing Escherichia coli in food: Incidence, ecology, and detection strategies in the United States. Food Control 59: 407-419.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Kwon, Y.M., S.C. Ricke, and R.K. Mandal. 2016. Transposon sequencing: Methods and expanding applications. Appl. Micro. Biotechnol. 100: 3143.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Chalova, V.I., J. Kim, P.H. Patterson, S.C. Ricke, and W.K. Kim. 2016. Reduction of nitrogen excretion and emission in poultry: A review for organic poultry. J. Environ. Sci. Health, Part B 51: 230-235.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Jarvis, N.A., C.A. OBryan, S.C. Ricke, M.G. Johnson, and P.G. Crandall. 2016. A review of minimal and defined media for growth of Listeria monocytogenes. Food Control 66: 256-269.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: ) Jarvis, N.A., C.A. OBryan, T. M. Dawoud, S. H. Park, Y.M. Kwon, P.G. Crandall, and S.C. Ricke. 2016. An overview of Salmonella thermal destruction during food processing and preparation. Food Control 68: 280-290.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Chalova, V.I., J.H. Kim, P.H. Patterson, S.C. Ricke, and W.K. Kim. 2016. Reduction of nitrogen excretion and emission in poultry: A review for conventional poultry. Worlds Poultry Sci. J. 72: 509-519.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Adam, B.D., R. Holcomb, M. D. Buser, B. Mayfield, J. Thomas, C.A. OBryan, P.G. Crandall, D. Knipe, R. Knipe, and S.C. Ricke. 2016. Enhancing food safety, product quality, and value-added in food supply chains using whole-chain traceability. The International Food and Agribusiness Management Review 19 (Issue A): 191-214.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Baker, C.A., P.M. Rubinelli, S.H. Park, and S.C. Ricke. 2016. Immuno-based detection of shiga toxin-producing pathogenic Escherichia coli  A review on current approaches and potential strategies for optimization. Crit. Revs. Microbiol. 42: 656675.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Roto SM, Y.M. Kwon and S.C. Ricke. 2016. Applications of in ovo technique for the optimal development of the gastrointestinal tract and the potential influence on the establishment of its microbiome in poultry. Front. Vet. Sci. 3:63. doi: 10.3389/fvets.2016.00063.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: OBryan, C.A., P. Crandall, D. Jaroni, S.C. Ricke and K.E. Gibson. 2017. Assessment of nitrogen and phosphorus loads present in environments impacted by alternative poultry processing operations. Renewable Agriculture and Food Systems. 32: 33-42
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Jarvis, N.A., J.R. Donaldson, C.A. OBryan, S.C. Ricke, and P.G. Crandall. 2017. Listeria monocytogenes infection of HD11, chicken macrophage-like cells. Poultry Sci. 96: 950-956.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Park, S.H., S. Roto, H. Pavlidis, D. McIntyre, K. Striplin, L. Brammer, and S.C. Ricke. 2017. Effects of feeding Original XPCTM to broilers with a live coccidiosis-vaccine under industrial conditions: Part 2. Cecal microbiota analysis. Poultry Sci. 96: 24002411.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Roto, S.M., S.H. Park, S.I. Lee, P. Kaldhone, H.O. Pavlidis, S.B. Frankenbach, D.R. McIntyre, K. Striplin, L. Brammer, and S. C. Ricke. 2017. Effects of feeding Original XPCTM to broilers with a live coccidiosis-vaccine under industry conditions: Part 1. Growth performance and Salmonella inhibition. Poultry Sci. 1831-1837.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Park, S,H., A. Perrotta, I. Hanning, S. Diaz-Sanchez, S. Pendleton, E. Alm, and S.C. Ricke. 2017. The chicken gut microbiome changes in response to prebiotics and plum fibers. Poultry Sci. 96: 1820-1830.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Dawoud, T.M., A. Khatiwari, S.H. Park, M.L. Davis, C.A. Baker, S.C. Ricke, and Y.M. Kwon. 2017. Heat survival and phenotype microarray profiling of Salmonella Typhimurium mutants. Curr. Microbiol. 74:257267.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Kim, S.A., S.H. Park, S.I. Lee, and S.C. Ricke. 2017. Development of a rapid method to quantify Salmonella Typhimurium using a combination of MPN with qPCR and a shortened time incubation. Food Microbiology 65: 7-18.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Rubinelli, P.M., S.A. Kim, S.H. Park, S.M. Roto, and S.C. Ricke. 2017. Sodium bisulfate and a sodium bisulfate/tannin mixture decreases pH when added to an in vitro incubated poultry cecal or fecal contents while reducing Salmonella Typhimurium marker strain survival and altering the microbiome. J. Environ. Sci. and Health, Part B 52: 607-615.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Park, S.H., S.A. Kim, P.M. Rubinelli, S.M. Roto and S.C. Ricke. 2017. Microbial compositional changes in broiler chicken cecal contents from birds challenged with different Salmonella vaccine candidate strains. Vaccine 35: 3204-3208.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Yang, Y., S.C. Ricke, G. Tellez, and Y.M. Kwon. 2017. Quantitative tracking of Salmonella Enteritidis transmission routes using barcode-tagged isogenic strains in chickens  Proof of concept study. Frontiers in Vet. Sci. 4:15. doi: 10.3389/fvets.2017.0001.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Park, S.H., S.A. Kim, S.I. Lee, P.M. Rubinelli, S.M. Roto, H.O. Pavlidis, D.R. McIntyre, and S.C. Ricke. 2017. Original XPCTM effect on Salmonella Typhimurium and cecal microbiota from three different ages of birds when incubated in an anaerobic in vitro culture system. Frontiers in Microbiol. 8: (Article 1070). doi 10.3389/fmoicb.2017 01070.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Jarvis, N.A., C.A. O'Bryan, E.M. Martin, S.C. Ricke, M.G. Johnson, and P.G. Crandall. 2017. Further evidence of how unbuffered starvation at 4 ?C influences Listeria monocytogenes EGD-e, HCC23, F2365, and Scott A. J. Food Prot. 80: 1749-1759.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Rubinelli, R., S.A. Kim, S.H. Park, C.A. Baker, and S.C. Ricke. 2017. Characterization of single and double Salmonella methionine auxotroph strains for potential vaccine use in poultry. Frontiers in Veterinary Science 4 (Article 103): doi: 10.3389/fvets2017.00103.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Park, S.H., S.I. Lee, K. Christensen, and S.C. Ricke. 2017. Effect of commercial prebiotic on gastrointestinal microbial population and growth performance of broilers. PLoS ONE 12(8): e0182805. https://doi.org/10.1371/ journal.pone.0182805.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Kim, S.A., S.H. Park, S.I.. Lee, and S.C. Ricke. 2017. Rapid and simple method by combining FTATM card DNA extraction with two set multiplex PCR for simultaneous detection of non-157 Shiga toxin-producing Escherichia coli strains and virulence genes in food samples. Letters in Applied Microbiology 65: 482-488.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Kaldhone, P., B. Khajanchi, J. Han, R. Nayak, S.C. Ricke, and S.L. Foley. 2017. Draft genome sequences of Salmonella enterica isolates containing incompatibility group I1 plasmids from swine, poultry and human sources. Genome Announcement 5 no. 39 e01056-17, doi: 10.1128/genomeA.01056-17.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Rubinelli, P.M., S.A. Kim, S.H. Park, S.M. Roto, N.J. Nealon, E.P. Ryan, and S.C. Ricke. 2017. Differential effects of rice bran cultivars to limit Salmonella Typhimurium in chicken cecal in vitro incubations and impact on the cecal microbiome and metabolome. PLoS ONE 12(9): e0185002. https://doi.org/10.137/journal.pone0185002.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Rajan, K, Z. Shi, and S.C. Ricke. 2017. Potential application of risk strategies for understanding Salmonella contamination in ground poultry products. Crit. Revs. Microbiol. 43: 370-392.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Varela-Stokes, A.S., S.H. Park, S.A. Kim, and S.C. Ricke. 2017. Microbial communities in North American Ixodid ticks of veterinary and medical importance. Frontiers in Veterinary Science 4: Article 179. doi 10.3389/fvets.2017.00179.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Wu, S., S.C. Ricke, K.R. Schneider, and S. Ahn. 2017. Food safety hazards associated with ready-to-eat cookie dough and its ingredients. Food Control 73:986993.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Dawoud, T.M., M.L. Davis, S.H. Park, S.A. Kim, Y.M. Kwon, N. Jarvis, C. OBryan, Z. Shi, P.G. Crandall, and S.C. Ricke. 2017. Salmonella thermal resistance - Molecular responses. Frontiers in Veterinary Science 4: (Article 93) doi: 10.33889/fvets.2017.00093.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Rothrock Jr., M.J., M.L. Davis, A. Locatelli, A. Bodie, T.G. McIntosh, and S.C. Ricke. 2017. Listeria occurrence in poultry flocks: Detection and potential implications. Frontiers in Veterinary Science 4 (Article 125) doi: 10.3389/fvets.2017.00125.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Ricke, S.C. 2017. Insights and challenges of Salmonella infections in laying hens. Current Opinions in Food Science 18: 43-49.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Baxter, M., B. Hargis, J.D. Latorre, S. Ricke, S.H. Park, S. Dridi, L. Greene, S. Bickler, R. Merino, and G. Tellez. 2017. Evaluating the effect of corn and/or rye based diets on gut health, bone strength and body weight in modern broiler chickens. International Poultry Scientific Forum, Atlanta, GA, Jan. 30-31st.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Baxter, M., J.D. Latorre, N. Anthony, S. Dridi, D.A. Koltes, S.C. Ricke, S. Park, E.S. Greene, R. Merino, S. Bickler, B.M. Hargis, and G. Tellez. 2016. Nutritional rehabilitation responses in two genetic lines of chickens. Symposium on Gut Health in Production of Food Animals, St Louis, MO., Nov. 14-16.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Lankford, S., S. Ricke, and D.R. Thompson. 2017. Simulating foodborne pathogens in poultry production and processing to defend against intentional contamination, Arkansas Academy of Science (AAoS) 10th Annual Meeting, University of Central Arkansas, Conway, AR., Apr 7.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Ricke, S. S.I. Lee, S.H. Park, K. Hiett, H. Sellers, and M. Rothrock. 2017. Microbial population analysis of broilers in different flock and embryonic ages from GI, yolk and egg wash via Illumina Miseq and QIIME pipeline. International Poultry Scientific Forum, Atlanta, GA, Jan. 30-31st.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Ricke, S., S.I. Lee, S.H. Park, S. Roto, P. Rubinelli, H. Pavlidis, and D. McIntyre. 2017. Impact of incubation time and addition of original-XPC on the microbiota in an in vitro broiler cecal bioassay. International Poultry Scientific Forum, Atlanta, GA, Jan. 30-31st.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Rubinelli, P., S.H. Park, H. Pavlidis, D. McIntyre, and S. Ricke. 2017. Inhibition of Campylobacter jejuni by original XPCTM in an anaerobic mixed fecal in vitro culture system. International Poultry Scientific Forum, Atlanta, GA, Jan. 30-31st.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Park, S.H., S.A. Kim, P. Rubinelli, C.A. Baker, and S.C. Ricke. 2017. Effect of Salmonella vaccine strains on broiler chicken cecal microbiota. International Assoc. Food Prot. Annual Meeting, Tampa, FL July. 9-12.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Kim, S.A., S.H. Park, S.I. Lee, and S.C. Ricke. 2017. A novel Salmonella Typhimurium quantification approach in a mixed cell culture spiked in chicken breast. International Assoc. Food Prot. Annual Meeting, Tampa, FL July. 9-12.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Shi, Z., P.M. Rubinelli, and S.C. Ricke. 2017. Poultry serovar Salmonella biofilm formation at the air-liquid interface in media of differing compositions and environmental incubation conditions. Poultry Science Association Annual Meeting, Orlando, FL, July 16th -20th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Kim, S.A. S.H. Park, A.D. Wolfenden, B.M. Hargis, H.O. Pavlidis, D.R. McIntrye, and S.C. Ricke. 2017. Characterization of mixed bacterial populations in chicken ceca recovered from colonies on Campylobacter selective commercial media using 16S rRNA next generation sequencing. Poultry Science Association Annual Meeting, Orlando, FL, July 16th -20th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Baxter, M., J.D. Latorre, S.H. Park, S. Ricke, X. Sun, B. Hargis and G. Tellez. 2017. Relationship between the microbiome in different sections of the gastrointestinal tract of broiler chickens fed a corn versus a rye based diets. Poultry Science Association Annual Meeting, Orlando, FL, July 16th -20th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Yang, Y., J.D. Latorre, Y. Kwon, P.M. Ray, X.H. Velasco, B.M. Hargis, G.I. Tellez, and S.C. Ricke. 2017. Salmonella excludes Salmonella in poultry: Confirming an old paradigm using conventional and barcode-tagged isogenic strains. 9th Annual Arkansas Association Protection Educational Conference, Holiday Inn, Springdale, AR., Sept. 19-21.


Progress 10/01/15 to 09/30/16

Outputs
Target Audience:Academic researchers at other universities; federal institutions (FDA and USDA); food safety industry, in general Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?I graduated one MS student that was working on a handwashing project using a norovirus surrogate, and she published 3 papers on this topic. My PhD student was also awarded a fellowship from NoroCORE for her proposal on norovirus and surrogate interactions with bacteris present in the environment. She will be funded on this fellowship until May 2017. I have also been able to receive additional funding based on the data generated by my students. I have recruited another PhD student that started Fall 2016, and she is working on the interactions of human noroviruses and its surrogates with various components present in the leafy green phyllosphere and aspects of viral inactivaiton and/or persistence. This work will be immediatley relevant to Goal #1. How have the results been disseminated to communities of interest?Results have been dessiminated via publications, presentations, and student theses. What do you plan to do during the next reporting period to accomplish the goals?Research in food safety at the University of Arkansas will continue to focus on evaluating control and prevention strategies of foodborne pathogens including human norovirus, Salmonella, and Campylobacter.

Impacts
What was accomplished under these goals? (1) Risk Assessment: Assess food safety risks in agriculture systems none (2) Risk Management: Develop science-based interventions to prevent and mitigate food safety threats Evaluation of prevention and control measures for human noroviruses and foodborne threats in general. We evaluated the impact of different soap types (foaming vs. gel-based) on handwashing efficacy and showed that foaming soaps remove bacteria significantly better than our virus surrogate, MS2 bacteriophage. In addition, we found that study participants wash their hands approximately 5 seconds longer with gel-based soaps when compared to foaming soaps. This is important to understanding that the right tools will aid in the control of norovirus transmission. Future research will be needed to either improve foaming soaps or educate others on the impact of hand soap selection with respect to microbial removal. We also evalauted the efficacy of another cleaining-in-place type food service dipper well with integrated ozone sanitizer. This version better aligns with the U.S. Food Code, and the results show significantly better inactivation of bacterial and viral surrogates on the food service utensil as well as with in the system itself (water and surfaces) when compared to a conventional dipper well. (3) Risk Communication: Communicate food safety messages to stakeholders I was invited to give presentations to both the Mid-Continental Association for Food and Drug Officials and the FDA Food/Feed Rapid Response Team on the topic of preventtion and control of human noroviruses and our handwashing work was included as a topic.

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Almeida G, Gibson KE. Evaluation of a recirculating dipper well combined with ozone sanitizer for control of foodborne pathogens in food service operations Journal of Food Protection. 79:1537-1548. 2016.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Conover DM, Gibson KE. Comparison of two plain soap types for removal of bacteria and viruses from hands with specific focus on food service environments Food Control. 69:141-146. 2016.


Progress 10/01/14 to 09/30/15

Outputs
Target Audience:Academic researchers at other universities; federal institutions (FDA and USDA); food safety industry, in general; and national and international poultry and egg commodity groups. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Communication has been primarily through conference presentations, invited seminars/presentations, and publications. What do you plan to do during the next reporting period to accomplish the goals?Research in food safety at the University of Arkansas will continue to focus on evaluating control and prevention strategies of foodborne pathogens including human norovirus and Salmonella

Impacts
What was accomplished under these goals? 1) Risk Assessment: Assess food safety risks in agricultural systems Gibson:Assessment of on-farm food safety perceptions and practices related to various strawberry production systems in Arkansas, Missouri and Oklahoma 2) Risk Management: Develop science-based interventions to prevent and mitigate food safety threats Crandall: With co-researchers developing an application for whole chain traceability of foods. Developing novel antimicrobials against important foodborne pathogens. Developing training modules for food workers in both the manufacturing and retail sectors. Gibson: Evaluation of prevention and control measures forhuman noroviruses. Evaluation of novel equipment for control of foodborne pathogens in food service environments. Kwon: Molecular methods and mutagenesis approaches for Salmonella and other foodborne pathogens. Ricke: Working with industry and commodity groups to develop effective preharvest intervention approaches for controlling Salmonella colonization in poultry broilers and layers as well as on plastic srufaces and other food storage container surfaces.

Publications

  • Type: Books Status: Published Year Published: 2015 Citation: Ricke, S.C., J.R. Donaldson, and C.A. Phillips (Eds.) 2015. Food Safety: Emerging Issues, Technologies and Systems. Academic Press, Elsevier, Inc., Oxford, UK, 449 pp.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Hanning, I., J. Lingbeck, and S.C. Ricke. 2015. Cardiovascular health and disease prevention: association with foodborne pathogens and potential benefits of probiotics. In: Bioactive Foods in Promoting Health: Probiotics-Prebiotics, 2nd Edition. R.R. Watson and V.R. Preedy (Eds.) Elsevier, San Diego, CA. pp. 793-806.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Ricke, S.C. 2015. Chapter 19. Application of anaerobic microbiology laboratory training and writing comprehension to food safety education. In: S.C. Ricke, J.R. Donaldson, and C.A. Phillips (Eds.) Food Safety: Emerging Issues, Technologies and Systems. Elsevier, Oxford, UK, pp. 395-419.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Ricke, S.C., J. Rivera Calo, and P. Kaldhone. 2015. Chapter 7. Salmonella control in food production  Current issues and perspectives in the United States. In: S.C. Ricke, J.R. Donaldson, and C.A. Phillips (Eds.) Food Safety: Emerging Issues, Technologies and Systems. Elsevier, Oxford, UK, pp. 107-133
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Handley, J.A., S.H. Park, Z. Shi, T.M. Dawoud, Y.M. Kwon, and S.C. Ricke. 2015. Chapter 6. Salmonella and the potential role for microbial process indicators on chicken carcasses. In: S.C. Ricke, J.R. Donaldson, and C.A. Phillips (Eds.) Food Safety: Emerging Issues, Technologies and Systems. Elsevier, Oxford, UK, pp. 81-104.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Ricke, S.C., T.M. Dawoud, and Y.M. Kwon. 2015. Chapter 4. Application of molecular methods for traceability of foodborne pathogens in food safety systems. In: S.C. Ricke, J.R. Donaldson, and C.A. Phillips (Eds.) Food Safety: Emerging Issues, Technologies and Systems. Elsevier, Oxford, UK, pp. 37-63.
  • Type: Book Chapters Status: Accepted Year Published: 2015 Citation: OBryan, C.A., P.G. Crandall, and S.C. Ricke. 2015. Chapter 9  A case study of an outbreak of Salmonella Enteritidis associated with shell eggs and egg containing products. In: Jan Mei Soon, Louise J. Manning and Carol A. Wallace (Eds.). Foodborne Diseases: Case studies of outbreaks in the agri-food industries. CRC Press/Taylor & Francis Group, Boca Raton, FL. (Accepted).
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Jarvis, N.A., P.G. Crandall, C.A. OBryan, and S.C. Ricke. 2015. Chapter 8. Antimicrobial resistance in Listeria spp. In: Chin-Yi Chen, Xianghe Yan, and Charlene Jackson (Eds.). Antimicrobial Resistance and Food Safety: Methods and Techniques. Academic Press/Elsevier Science, Amsterdam, The Netherlands. pp. 137-153.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Ricke, S.C. and J.Rivera Calo. 2015. Chapter 3. Antibiotic resistance in pathogenic Salmonella. In: Chin-Yi Chen, Xianghe Yan, and Charlene Jackson (Eds.). Antimicrobial Resistance and Food Safety: Methods and Techniques. Academic Press/Elsevier Science, Amsterdam, The Netherlands. pp. 37-53.
  • Type: Book Chapters Status: Accepted Year Published: 2015 Citation: Ricke, S.C. 2015. Ecology of Salmonella and microbial contamination in postharvest table shell egg production. In: Anderson de Souza SantAna (Ed.) Quantitative Microbiology in Food Processing  Modeling the Effects of Processing Operations in Microbial Ecology of Foods. Wiley-Blackwell, Oxford, UK. (Accepted).
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: OBryan, C.A., E.S. Whaley, S.R. Milillo, P.G. Crandall, M.G. Johnson, and S.C. Ricke. 2015. Chapter 15. Use of bacteriocins and essential oils for the control of Listeria monocytogenes in processed foods. In: Microbial Food Safety and Preservation Techniques, Eds. V Ravishankar Rai and A Jamuna Bai, CRC Press/Taylor & Francis Group, Boca Raton, FL., pp. 287-304.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: OBryan, C.A., P.G. Crandall, S.C. Ricke, and J.B. Ndahetuye. 2015. Chapter 7. Lactic acid bacteria as antimicrobials in food products: analytical methods and applications. In Handbook of Natural Antimicrobials for Food Safety and Quality. Matt Taylor (Ed.), Woodhead Publishing Ltd., Cambridge, UK. pp. 137-151.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: OBryan, C.A., P.G. Crandall, S.C. Ricke, and J.B. Ndahetuye. 2015. Chapter 6. Lactic acid bacteria as antimicrobials in food products: types and mechanisms of action. In Handbook of Natural Antimicrobials for Food Safety and Quality. Matt Taylor (Ed.), Woodhead Publishing Ltd., Cambridge, UK. pp 117-136.
  • Type: Book Chapters Status: Awaiting Publication Year Published: 2015 Citation: Gibson KE and Borchardt MA. 2016 Methods for Virus Recovery in Water In Press In: Cannon, JL and Goyal SM (eds), Viruses in Food, 2nd edition. Springer US.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Gibson KE. 2015 "Tracking Pathogens in the Environment: Applications to Fresh Produce Production" pp. 17-35 In: Ricke SC, Donaldson JR, Phillips CA (eds), Food Safety: Emerging Issues, Technologies and Systems. Elsevier US.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Sirsat SA, Gibson KE, Neal JA. 2015 "Food Safety at Farmers Markets: Fact or Fiction?" pp. 319-327 In: Ricke SC, Donaldson JR, Phillips CA (eds), Food Safety: Emerging Issues, Technologies and Systems. Elsevier US.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: Kwon, Y. M., S. C. Ricke, and R. K. Mandal. 2015. Transposon sequencing: methods and expanding applications. Applied Microbiology and Biotechnology [Epub ahead of print]
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Jiang, T., R. F. Wideman Jr., A. Khatiwara, I. Pevzner, and Y. M. Kwon. 2015. Molecular survey of bacterial communities associated with bacterial chondronecrosis with osteomyelitis (BCO) in broilers. PLOS one, 10(4):e0124403.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Wideman Jr., R. F., A. Al-Rubaye, Y. M. Kwon, J. Blankenship, H. Lester, K. N. Mitchell, I. Y. Pevzner, T. Lohrmann, and J. Schleifer. 2014. Prophylactic administration of a combined prebiotic and probiotic, or therapeutic administration of enrofloxacin, to reduce the incidence of bacterial chondronecrosis with osteomyelitis in broilers. Poultry Science 94, 25-36.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: Baker, C.A., P.M. Rubinelli, S. H. Park, F. Carbonero, and S.C. Ricke. 2015. Shiga toxin-producing Escherichia coli in food: Incidence, ecology, and detection strategies in the United States. Food Control doi:10.1016/j.foodcont.2015.06.011
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Roto, S.M., P. M. Rubinelli, and S.C. Ricke. 2015. An introduction to the avian gut microbiota and the effects of yeast-based prebiotic compounds as potential feed additives. Front. Vet. Sci. 2 (Article 28): 1-18. doi: 10.3389/fvets.2015.00028.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: OBryan, C.A., S.J. Pendleton, P.G. Crandall, and S.C. Ricke. 2015. Potential of plant essential oils and their components in animal agriculture  in vitro studies on antibacterial mode of action. Front. Vet. Sci. 2 (Article 35): 1-8. doi: 10.3389/fvets.2015.00035
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: Herzig, G.P.D., M. Aydin, S. Dunigan, P. Shah, K. C. Jeong, S.H. Park, S.C. Ricke, and S. Ahn. 2015. Magnetic bead-based immunoassay coupled with tyramide signal amplification for detection of Salmonella in foods. J. Food Safety (In press).
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Howton, J., E. Keifer, C.A. Murphy, S. Sirsat, C.A. OBryan, S.C. Ricke, P.G. Crandall, and J. Neal. 2016. A comparison of online food safety training programs using the customizable tool for online training evaluation. Food Control 59: 82-87.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Baker, C.A., S.H. Park, S.A. Kim, P.M. Rubinelli, S.M. Roto, S.I. Lee, S.Ramsaroop, M. Miller, and S.C. Ricke. 2015. Formalin-fixed cells as an internal standard approach for the detection and quantitative assessment of shiga toxin-producing Escherichia coli (STEC). Food Control (Accepted).
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Sanad, Y.M., K. Johnson, S.-H. Park, B. Shaheen, J. Han, S.L. Foley, P.B. Kenney, P.B., S. Ricke, and R. Nayak. 2015. Molecular characterization of Salmonella enterica serovars isolated from a turkey production facility in the absences of selective antimicrobial pressure. Foodborne Pathogens and Disease (Accepted).
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: Lee, S.I., S.H. Park, and S.C. Ricke. 2015. Assessment of cecal microbiota, integrons, fermentation, and Salmonella frequency in conventionally raised broilers in response to a commercial prebiotic. Poultry Sci. (In press).
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: Baker, C.A., P.M. Rubinelli, S.H. Park, and S.C. Ricke. 2015. Immuno-based detection of shiga toxin-producing pathogenic Escherichia coli  A review on current approaches and potential strategies for optimization. Crit. Revs. Microbiol. (In press).
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Ricke, S.C. 2015. Potential of fructooligosaccharide prebiotics in alternative and nonconventional poultry production systems. Poultry Sci. 94:14111418.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Clayborn, J., J. Adams, C.A. Baker and S.C. Ricke. 2015. Assessment of Salmonella spp. attachment to reusable plastic containers based on scanning electron microscopy and BAX� PCR. J. Food Res. 4: 1-10.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Rivera Calo, J., C.A. Baker, S.H. Park, and S.C. Ricke. 2015. Specificity of Salmonella Typhimurium strain (ATCC 14028) growth responses to Salmonella serovar-generated spent media. J. Environ. Health Part B 50: 423429.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Rubinelli, P.M., S.I. Lee, S.M. Roto, S.H. Park, and S.C. Ricke. 2015. Regulated expression of virulence gene mviN provides protective immunity and colonization control of Salmonella in poultry. Vaccine 33: 53655370.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: Park, S.H., S.E. Dowd, J.L. McReynolds, J.A. Byrd, D.J. Nisbet, and S.C. Ricke. 2015. Evaluation of feed grade sodium bisulfate impact on gastrointestinal tract microbiota ecology in broilers via a pyrosequencing platform. Poultry Sci. (In press).
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Kwak, H.-L., S.-K. Han, S. Park, S. H. Park, J.-Y. Shim, M. Oh, S.C. Ricke, and H.-Y. Kim. 2015. Development of a rapid and accurate identification method for Citrobacter species isolated from pork products using a Matrix-Assisted Laser-Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). Journal of Microbiology and Biotechnology 25: 1537-1541.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Park, S.H. and S. C. Ricke. 2015. Development of multiplex and quantitative PCR assays for simultaneous detection of Salmonella genus, Salmonella subspecies I, S. Enteritidis, S. Heidelberg, and S. Typhimurium. J. Appl. Microbiol. 118, 152-160.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Milillo, S.R., B. Lungu, C. A. OBryan, S.E. Dowd, A. Muthaiyan, M. G. Johnson, P.G. Crandall, and S. C. Ricke. 2015. Listeria monocytogenes batch culture growth response to metabolic inhibitors. J. Environ. Sci. Health, Part B, 50: 146-150.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Beers, K., J. Ferguson, S. H. Park, P. Cook, C.A. Baker , M. Miller, D. Caldwell, S. Ramsaroop, and S. C. Ricke. 2015. Comparison of the RAPID-B� flow cytometer and the BAX� system for the detection of non-O157 shiga toxin-producing Escherichia coli (STEC) in beef products. Food Control 50: 72-75.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Mertz AW, OBryan CA, Crandall PG, Ricke SC, Morawicki R, The elimination of Listeria monocytogenes attached to stainless steel or aluminum using multiple hurdles Journal of Food Science 80: 1557-1562. IF 1.791
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Pellegrino R, Crandall PG, Seo HS. 2015. Hand washing and disgust response to handling different food stimuli between two different cultures. Food Res Int 76: 301-308. IF 2.818
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Samant S, Crandall PG, O'Bryan CA, Lingbeck J, Martin E, Seo HS. 2015. Sensory impact of chemical and natural antimicrobials on poultry products: A Review. Poultry Science 94:16991710. IF 1.544
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Lingbeck JM, OBryan CA, Martin EM, Adams JP, Crandall PG. 2015. Sweetgum: an ancient source of beneficial compounds with modern benefits Pharmacognosy Reviews 9, doi: 10.4103/0973-7847.156307
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Jarvis N, OBryan CA, Ricke SC, Crandall PG. 2015. The functionality of plum ingredients in food products: a review Meat Science 102: 41-48.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Park SH, Gibson KE, Almeida G, Ricke SC. Assessment of gastrointestinal microflora in pasture raised chickens fed two commercial prebiotics Journal of Probiotics and Health. 2: 122. 2014.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Arthur SE, Gibson KE. Comparison of methods for evaluating the thermal stability of human enteric viruses and their surrogates Food and Environmental Virology. 7: 14-26. 2014.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Gibson KE and Almeida G. Comparison of a continuous flow dipper well and a reduced water dipper well combined with ultraviolet radiation for control of microbial contamination. Food Control. 47: 301-305. 2015.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Haack SK, Duris JW, Kolpin DW, Fogarty LR, Johnson HE, Gibson KE, Focazio M, Schwab KJ, Hubbard LE, Foreman WT. "Genes indicative of zoonotic and swine pathogens are persistent in stream water and sediment following a swine manure spill" Applied and Environmental Microbiology. 81: 3430-3441. 2015.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Hsueh TY, Gibson KE. Investigation of human norovirus surrogate interactions with Acanthamoeba spp. Applied and Environmental Microbiology. 81: 4005-4013. 2015.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Hsueh TY, Gibson KE. Transfer of Acanthamoeba spp. associated with viruses to fresh produce from water and environmental surfaces Letters in Applied Microbiology. 61:192-198. 2015
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Rivera Calo J, Crandall PG, OBryan CA, Ricke SC. 2015. Essential oils as antimicrobials in food systems A review Food Control 54: 111-118.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Crandall PG, OBryan CA, Peterson R, Dyenson N, Yiannas F. 2015. A survey estimating the benefits of incorporating Listeria specific growth inhibitors in bulk luncheon meats to be sliced in retail delis. Food Control 53: 185-188.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Arthur SE, Gibson KE. Physicochemical stability profile of Tulane virusa human norovirus surrogate Journal of Applied Microbiology. 119:828-875. 2015.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Wright K, Sirsat SA, Neal JA, Gibson KE. Growth of local food systems: a review of potential food safety implications. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources. 10:1-13. 2015.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Ghosh A, Ricke SC, Almeida G, Gibson KE*. Application of essential oil compounds and bacteriophage to control Staphylococcus aureus. Current Microbiology. Accepted 11/12/15. 2015.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Conover DM, Gibson KE. A review of methods for the evaluation of handwashing efficacy Food Control. 2015. doi: 10.1016/j.foodcont.2015.11.020
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Arthur SE, Gibson KE*. Environmental persistence of Tulane virusa surrogate for human norovirus Canadian Journal of Microbiology. 2015. doi: 10.1139/cjm-2015-0756
  • Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: 115th American Society for Microbiology General meeting, New Orleans, LA. May 2015 Poster Presentation: Genome-wide genetic requirements of Salmonella Typhimurium for optimal growth in the presence of propionate Authors: Mandal, R.K., T. Jiang, and Y.M. Kwon.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: International Association for Food Protection, Portland,OR July 2015 Poster Presentation: Evaluation of Pathogen Survival in Fresh Water Sediments and Implications for Irrigation Water Quality Authors: Kristen E. Gibson, Giselle Almeida, Danielle Conover, Anisha Ghosh, Sabastine Arthur, Tun-Yun Hsueh, Jay Jackson, Lauren Smith
  • Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: International Association for Food Protection, Portland, OR July 2015 Poster Presentation: Combined Efficacy of Essential Oil Compounds and Bacteriophage to Control Staphylococcus aureus with Potential Application Authors: Anisha Ghosh and Kristen E. Gibson
  • Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: International Association for Food Protection, Portland, OR July 2015 Poster Presentation: Farmers Market Booths: Does the Layout Affect Microbial Cross-contamination? Authors: Kristen Kirkwood, Karla Acosta, Kristen Gibson, Sujata Sirsat, Jack Neal
  • Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: 115th American Society for Microbiology General meeting, New Orleans, LA. May 2015 Oral Presentation: Monitoring Salmonella transmission within a chicken flock using barcode-tagged strains Authors: Yang, Y., and Y.M. Kwon.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Poultry Science Association Annual Meeting, Louisville, KY. July 2015 Oral Presentation: Understanding transmission dynamics of Salmonella Enteritidis in a chicken flock using barcoded strains Authors: Yang, Y., and Y.M. Kwon.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Roto, S., S.I. Lee, P. Rubinelli, S. H. Park, and S. Ricke. 2015. Cecal Salmonella and indigenous microflora response to original XPCTM-fed broilers. International Poultry Scientific Forum, Atlanta, GA, Jan. 26-27th.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Park, S.H., F.G. Carbonero, and S.C. Ricke. 2015. Evaluation of microbial populations in chicken ceca raised on pasture flock fed with commercial prebiotics via sequencing (Illumina MiSeq). International Assoc. of Food Protection Annual Meeting, Portland, OR.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Baker, C.A., S.H. Park, and S.C. Ricke. 2015. Quantification and comparison of Salmonella Typhimurium and Heidelberg biofilm formation on plastic coupons. International Assoc. of Food Protection Annual Meeting, Portland, OR
  • Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Rubinelli, P.M., S.I. Lee*, S.H. Park, D. Korver and S.C. Ricke. 2015. Impact of different prebiotics on a Salmonella Enteritidis marker strain in rooster in vitro cecal incubations and on in vivo microbiota from layer hen cecal contents. Poultry Science Association Annual Meeting, Louisville, KY, July 27-29th
  • Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Rubinelli, P.M. and S.C. Ricke. 2015. Effect of sodium bisulfate and a sodium bisulfate/tannin mixture on Salmonella Typhimurium marker strain survival in adapted and unadapted anaerobic in vitro mixed cultures. Poultry Science Association Annual Meeting, Louisville, KY, July 27-29th
  • Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Rubinelli, P.M. and S.C. Ricke. 2015. Original XPCTM decreases Salmonella Typhimurium and Enteritidis populations and alters fermentation acid profiles in chicken in vitro cecal incubations. Poultry Science Association Annual Meeting, Louisville, KY, July 27-29th


Progress 10/01/13 to 09/30/14

Outputs
Target Audience: Target audiences: Academic researchers in other universities;federalinstitutions (FDA and USDA) and natioanl and international poultry and egg commodites groups Efforts: Academic seminars to commodity groups and other institututions Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? 1) Risk Assessment: Assess food safety risks in agricultural systems Gibson: Project funded by the Center for Produce Safety involves the evaluation of pathogen, surrogate, and indicator organism survival in fresh water sediments and the potential impact on irrigation water quality sampling programs. The primary goal is determine the best indicators and surrogates for determining water quality for each pathogen of concern including Salmonella, shiga toxin-producing E. coli, norovirus, and Listeria monocytogenes. 2) Risk Management: Develop science-based interventions to prevent and mitigate food safety threats Crandall: With co-researchers developing an application for whole chain traceability of foods. Developing novel antimicrobials against important foodborne pathogens. Developing training modules for food workers in both the manufacturing and retail sectors. Gibson: Evaluation of reduced water dipper well with integrated UV radiation step for cleaning and sanitizing serving utensils at the point of service. System developed by outside company. Kwon: Molecular methods and mutagenesis approaches for Salmonella and other foodborne pathogens. Ricke: Working with industry and commodity groups to develop effective preharvest intervention approaches for controlling Salmonella colonization in poultry broilers and layers.

Publications

  • Type: Journal Articles Status: Published Year Published: 2014 Citation: 1) Gibson K.E.. 2014. Virus pathogens in water: Occurrence, public health impact, and available control strategies. Current Opinion in Virology. 4: 50-57. 2014. 2) Killian SA, D.E. Beck, C.A. OBryan, N. Jarvis, E.C. Clausen, and P.G. Crandall. 2014. Student centered and dynamic interfaces that enrich technical learning for on-line science learnersa review of the literature. J Food Sci Ed 13: 47-56. 3) Koo, O.K., J.B. Ndahetuye, C.A. OBryan, S.C. Ricke, and P.G.Crandall. 2014. Influence of Listeria innocua on the attachment of Listeria monocytogenes to stainless steel and aluminum surfaces. Food Control 39: 135-138. 4) Kushwaha K, C.A. OBryan, D. Babu, P.G. Crandall, O. Chen, and S.O. Lee. 2014. Human health effects of isoflavones from soybeans. Agriculture, Food and Analytical Bacteriology, 4: 122-142. 5) Lingbeck, J.M., P. Cordero, C.A OBryan, M.G Johnson, S.C. Ricke, and P.G. Crandall. 2014. Functionality of liquid smoke or condensed smoke as an all-natural antimicrobial. Meat Sci. 97: 197206. 6) Lingbeck JM, P. Cordero, C.A. OBryan, M.G. Johnson, S.C. Ricke, and P.G. Crandall. 2014. Temperature effects on the antimicrobial efficacy of condensed smoke and lauric arginate against Listeria and Salmonella. J Food Prot 77: 934-940. 7) Luj�n-Rhenals D.E., R. Morawicki, and S.C. Ricke. 2014. Tolerance of S. cerevisiae and Z. mobilis to inhibitors produced during dilute acid hydrolysis of soybean meal. J. Environ. Sci. Health, Part B. 49: 305311. 8) Masuku, S.M., D. Babu, E.M. Martin, C.A. OBryan, P.G. Crandall, and S.C. Ricke. 2014. Lethality of moist heat and silver dihydrogen citrate sanitizer combinations on Listeria spp. adhered to components of a deli meat slicer. Food Control 44: 227-232. 9) Mertz, A.W. O K. Koo, C.A. OBryan, R. Morawicki, S.A.Sirsat, J.A. Neal, P.G. Crandall, and S.C. Ricke. 2014. Microbial ecology of meat slicers as determined by denaturing gradient gel electrophoresis. Food Control 42: 242-247. 10) Neal JA, C.A. OBryan, and P.G. Crandall. 2014. A personal hygiene behavioral change study at a Midwestern cheese production plant. Agric. Food Anal. Bacteriol. 4: 13-19. 11) Neal JA, C.A. OBryan, and P.G. Crandall. 2014. Preventing post-processing contamination in a food nugget processing line when language barriers exist J. Agric. Food Anal. Bacteriol. 4: 20-26. 12) OBryan CA, J.A. Neal, and P.G. Crandall. 2014. Behavioral change study at a Western soup production plant. Agric. Food Anal. Bacteriol. 4: 27-34. 13) Saengkerdsub, S., A. Muthaiyan , J.M. Lingbeck, C.A. OBryan, P.G. Crandall, and S.C. Ricke. 2014. Identification and methionine analog tolerance of environmental bacterial isolates selected on methionine analog containing medium. J. Environ. Sci. Health, Part B. 49: 290298. 14) Sirsat, S.A., K. Kim, K.E. Gibson, P.G. Crandall, S.C. Ricke, and J.A. Neal. 2014. Tracking microbial contamination in retail environments using Glo Germ" powder - A retail delicatessen environment example. Journal of Visualized Experiments 85: e51402. doi:10.3791/51402
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: 1) Adam, B.D., M.D. Buser, B. Mayfield, J. Thomas, A. Kumar, K. Palepu, P. Crandall, and S. Ricke. 2014. Whole-chain traceability in beef production  Information sharing from farm to fork and back again. Invited Presentation at the Southern Animal Health Association/National Association of State Meat and Food Inspection Directors - Eastern Region Meeting in Oklahoma City, Oklahoma, June 3. 2) Arthur S.E. and K.E. Gibson. 2014. Thermal inactivation of Tulane virus, a surrogate for the study of human norovirus. 6th Annual Arkansas Association for Food Protection Educational Conference - Exploration of Emerging Issues in Food Safety, Chancellor Hotel, Fayetteville, AR, Sept. 11-13. 3) Baker, C.A., Si H. Park, P. Rubinelli, S. Roto, and S.C. Ricke. 2014. An internal standard approach for the detection and enumeration of shiga toxin-producing Escherichia coli (STEC) by quantitative PCR (qPCR). From Abstract to Contract - Graduate Student Research and Creative Expression Competition. Graduate School, Mullins Library, University of Arkansas, Nov. 14. 4) Beers, K., J. Ferguson, M. Miller, D. Caldwell, S. Ramsaroop, P. Rubinelli, S. H. Park, P. Cook, and S. Ricke. 2014. Rapid detection of Non-O157 STEC E. coli using a flow cytometry-based pathogen detection system (Rapid-B Pathogen Detection System, Vivione Biosciences). International Assoc. Food Prot. Annual Meeting, Indianapolis, IN Aug. 3-6. 5) Crandall, P.G., R.E. Engler, III, C.A. OBryan, S.A. Killian, N. Jarvis, D.E. Beck and E. Clausen. 2014. Development of a virtual laboratory platform to teach the concepts of enzyme kinetics in food chemistry. Annual Meeting of the Institute of Food Technologists, June 21-24, New Orleans, LA. 6) Crandall, P.G., C.A. OBryan, S.A. Killian, N. Jarvis, D.E. Beck and E. Clausen. 2014. Comparison of an animation to a traditional, wet Laboratory for teaching the physical properties of ice. Annual Meeting of the Institute of Food Technologists, June 21-24, New Orleans, LA. 7) Frederick, B.N., N.A. Jarvis, C.A. OBryan, S.C. Ricke, and P.G. Crandall. 2014. Growth kinetics of pastured poultry Listeria spp. isolates. University of Arkansas Department of Food Science Undergraduate Research Competition, April 3. 8) Hsueh, T.-Y. and K.E. Gibson. 2014. Free living amoeba as reservoirs for the transmission of norovirus. International Assoc. Food Prot. Annual Meeting, Indianapolis, IN Aug. 3-6. 9) Jarvis, N.A., J.R. Donaldson, C.A. OBryan, S.C. Ricke, and P.G. Crandall. 2014. The interactions of the intracellular pathogen Listeria monocytogenes with the chicken macrophage-like cell line HD11. Poultry Science Association Annual Meeting, Corpus Christi, TX July, 14-17. 10) Jarvis N.A, C.A. OBryan, M.G. Johnson, S.C. Ricke, and P.G. Crandall. 2014. Rapid growth differences of two strains of Listeria monocytogenes in defined medium using glucose as the sole carbon source. International Assoc. Food Prot. Annual Meeting, Indianapolis, IN Aug. 3-6.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: 11) Lingbeck, J.M., P. Cordero, C.A. OBryan, Michael G Johnson, S.C. Ricke and P.G. Crandall. 2014. Reduction of L. monocytogeneson Ready to Eat Turkey Breast by Liquid Smoke Application. Institute of Food Technologists Annual Meeting and Food Expo., New Orleans, LA, June 21-24. 12) Lingbeck, J.M., P. Cordero, C.A. OBryan, Michael G Johnson, S.C. Ricke and P.G. Crandall. 2014. Effects of smoking and marination on extending the shelf life and sensory qualities of cooked chicken breasts. Institute of Food Technologists Annual Meeting and Food Expo., New Orleans, LA, June 21-24. 13) Liou, L., S.A. Sirsat, K.E. Gibson, and J.A. Neal. 2014. Food safety guidelines for farmers markets in the United States: A need for standardization. International Assoc. Food Prot. Annual Meeting, Indianapolis, IN Aug. 3-6. 14) Mertz, A.W., R. Morawicki, P. Crandall, and S. Ricke. 2014. The biofilm forming characteristics of L. monocytogenes and L. innocua that facilitate persistent contamination of food processing equipment. Institute of Food Technologists Annual Meeting and Food Expo., New Orleans, LA, June 21-24. 15) Park, S.H., S. I. Lee, C. A. Baker, and S.C. Ricke. 2014. Development of multiplex PCR for simultaneous detection of pathogenic Escherichia coli strains and select virulence gene. Poultry Science Association Annual Meeting, Corpus Christi, TX July, 14-17. 16) Rubinelli, P., S. H. Park, M. Miller, D. Caldwell, S. Ramsaroop, K. Beers, P. Cook, and S. Ricke. 2014. Development of a potential internal standard approach for comparing Flow Cytometry-Based (FCB) pathogen detection system (Rapid-B Pathogen Detection System, Vivione Biosciences) and quantitative PCR for enumeration of foodborne pathogenic Escherichia coli. International Assoc. Food Prot. Annual Meeting, Indianapolis, IN Aug. 3-6. 17) Sakul, R., K.E. Gibson�, J. Adams, G. Almeida, E.M. Martin, D.J. Carrier, and S.C. Ricke. 2014. Determination of partition coefficients of essential oil from Pinus teada L. extracts using centrifugal partition chromotography. 6th Annual Arkansas Association for Food Protection Educational Conference - Exploration of Emerging Issues in Food Safety, Chancellor Hotel, Fayetteville, AR, Sept. 11-13. 18) Shi, H., S.H. Park, N. Jarvis, J. Donaldson, P. Crandall, and S.C. Ricke. 2014. Antibiotic susceptibility of Listeria species isolated from conventional, pasture flock raised chickens and environments. International Assoc. Food Prot. Annual Meeting, Indianapolis, IN Aug. 3-6.