Source: UNIVERSITY OF NEBRASKA submitted to NRP
INVASIVE WOODY SPECIES IN GRASSLANDS AND RIPARIAN FORESTS OF NEBRASKA: IMPACTS ON ECOSYSTEM PROCESSES, RESILIENCE AND RESPONSE TO CLIMATE VARIABILITY AND CHANGE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1000916
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2013
Project End Date
Sep 30, 2018
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF NEBRASKA
(N/A)
LINCOLN,NE 68583
Performing Department
School of Natural Resources
Non Technical Summary
One of challenges we face today is understanding ecosystem services as impacted by anthropogenic management, and climate change, and how they feedback on ecosystem functions in grasslands and forests. Changes in ecosystem processes are occurring at unprecedented rates. Uncertainties related to the trajectory of these changes under changing climate are critical in a semi-arid environment where temperature, precipitation, and disturbance are key factors determining vegetation type, including transitions between grasslands and forests. In Nebraska, one of the consequences of these changes is woody species encroachment; like the encroachment of eastern redcedar (Juniperus virginiana) and ponderosa pine (Pinus ponderosa) into grasslands, and eastern redcedar and Russian olive (Eleagnus angustifolia)into cottonwood (Populus deltoides) riparian forests. This research aims at investigating the impacts of woody species invasion in semi-arid grasslands and riparian areas of NE on ecosystem functions with emphasis on ecohydrology, and ecosystem productivity and diversity, and determining environmental drivers, thresholds, and limitations to woody species growth under projected climate change. Specifically we will use: -Quantitative ecophysiological and ecological approaches to study the impacts of ponderosa pine and eastern redcedar expansionin grasslands, and eastern redcedar and Russian olive invasionin cottonwood riparian forests on plant diversity and productivity, water balance, and resources availability. -Dendroecological approaches to determine past and present growth and responses of invasive and nativewoody speciestothe environmentand to stand density. -Dendroecological, ecophysiological and environmental data to forecast future woody species distribution as impacted by climate variability and change. The knowledge gained will support the development of science based management guidelines to enhance the sustainability and integrity of Nebraska's resources and facilitate appropriate management decisions.
Animal Health Component
60%
Research Effort Categories
Basic
40%
Applied
60%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2030699102040%
1020210107030%
1210799107030%
Goals / Objectives
This research aims at investigating the impacts of woody species invasion in semi-arid grasslands and riparian areas of NE on ecosystem functions with emphasis on ecohydrology, and ecosystem productivity and diversity, and determining environmental drivers, thresholds, and limitations to woody species growth under projected climate change. Specifically we will use: -Quantitative ecophysiological and ecological approaches to study the impacts of ponderosa pine (Pinus ponderosa) and eastern redcedar (Juniperus virginiana)expansionin grasslands, and eastern redcedar and Russian olive (Eleagnus angustifolia)invasionin cottonwood (Populus deltoides) riparian forests on plant diversity and productivity, water balance, and resources availability. -Dendroecological approaches to determine past and present growth and responses of invasive and nativewoody speciestothe environmentand to stand density. -Dendroecological, ecophysiological and environmental data to forecast future woody species distribution as impacted by climate variability and change.
Project Methods
Sites: Site 1. Grasslands site located at the Nebraska National Forest (NNF). The NNF is an experimental forest established in 1902, hand-planted with , mainly ponderosa pine, in the 1930's, and later invaded by eastern redcedar. The documented site history and uniformity of plantations, age, and soils make this location uniquely suited to addresslong-term tree expansion in the Nebraska Sandhills. Eighteen plots were established between 1999 and 2002, of which, 9 have been designated as long-term study plots, ranging from grasslands to dense forests of eastern redcedar or ponderosa pine. Data on stand structure, tree age and growth, soil nutrient availability, root biomass, leaf area index, percentage penetration of radiation, soil water and microclimate (weather stations) have been collected. Site 2. Riparian forest, located at Bartley, Red Willow County, Nebraska, USA, along the Republican River, with a well-established overstory of the native eastern cottonwood, and an understory dominated by the invasive eastern redcedar, and Russian olive. Six plots were established in 2007 on this site, 3 cleared of understory invasive species and 3 were kept as controls. Species characteristics including average height, density, basal area, and percentage of total basal area for each of the species have been collected. Method: Objective 1: Use quantitative ecological and ecophysiological approaches to study the impacts of ponderosa pine and eastern redcedar site density in grasslands, and eastern redcedar and Russian olive site density in cottonwood riparian forests on plant diversity and productivity, water balance, and resources availability, and how these responses are impacted by climate variability and extremes. At the NNF's long-term plots, ranging from open grasslands to dense canopy forests, data collection will continue on quantifying trees and grasses responses to intra- and inter-annual climate variability and woody species density, and their responses and resilience to drought and extreme weather events. Parameters that have been and will continue to be measured are dynamics of soil moisture (monthly to depth 200 cm, theta probes Dynamax); site productivity, forage quality, and plant biodiversity (in June and August to capture maximum standing biomass in years 2 & 4 of the project); and ecophysiological responses including plant water relations (2 PMS instruments), gas exchange (e.g., photosynthetic rates, stomatal conductance, water use efficiency, chlorophyll fluorescence; 2 LICOR 6400), δ13C isotopes (intrinsic water use efficiency), and chlorophyll content (absorbance measurements). Ecophysiological variables are and will continue to be collected on trees in various stand densities and representative grasses in years 2 & 4 of the project, during the growing season and when weather permitting during winter. We will also process and analyze recently acquired sap flow data (study supported by current McStennis grant), to quantify tree water use and to develop models to determine tree density thresholds. Sap flow technique can be scaled to determine water consumption on a stand level. On Riparian sites, we will continue our data collection on understory vegetation dynamics, productivity, biodiversity, leaf area, in invaded and restored sites, and we will process and analyze data collected on tree level water use (sap flow techniques), together with data collected on understory evapotranspiration using surface renewal techniques, and modeled intercepted precipitation, to determine stand level water consumption in invaded and thinned (restored) plots. Data obtained from this objective are critical for determining responses to climate, thresholds, community resilience, and modeling and forecasting the future of vegetation dynamics and resilience in a changing climate. Objective 2. Use dendrochronological, and ecophysiologcal approaches to determine past and present growth and functional responses of trees in objective 1 to climate variability and change, and to species density. To complement data obtained in objective 1, we have been collaborating with a dendrochronology lab at the Federal Swiss Institute on the use of dendrochronological/ecological techniques or the study of tree rings of annual growth rates, together with ring δ13C and δ18O ratios (for both early and late wood) to determine past and present responses of trees to density (for threshold determination), climate variability, water availability, and stream flow (in the case of the riparian site). We will only conduct this study on site 1 (NNF) since site 2 (riparian) has already been sampled and manuscript is in preparation. Briefly cores obtained from trees at NNF (plots ranging from open to dense) will be mounted, dried, and scanned for initial analysis in Awada's lab. Samples will then be sent to the Dendro lab for rings growth and isotopes analyses. Ring-widths will be measured to the nearest 0.01 mm (time series analysis and presentation (TSAP) instrument and software package), cross-dated visually and statistically by Gleichläufigkeit, and verified with the program COFECHA. After crossdating is complete, age trends will be removed by eliminating the first seven or more years of core data when the invasive species were establishing. Data will then be standardized using the dendrochronology software ARSTAN. The raw tree ring width, standardized chronology, residual chronology, and yearly basal area chronology will be related to density and climatic factors using the DENDROCLIM2002 software and R packages. The correlations between tree ring width and independent variables (including density, average monthly precipitation, average monthly and average minimum and maximum temperatures, and PDSI values) will be used to build a mathematical model in objective 3. Objective 3. Utilize dendrochronological, ecophysiological and environmental data from 1 & 2 to model and forecast the response of trees and their future distribution as impacted by projected climate change scenarios We will use data from objective 1 & 2 to develop a hybrid model (process-based and empirical) to forecast the future growth and dynamics of the trees based on time series dendrochronological supported by physiological data together with daily data of maximum and minimum temperatures, precipitation, stream flow (for riparian site). We will establish the base temperature and the cumulative degree days used by individual species to annually grow. Precipitation together with hydraulic soil profile properties will be used to parameterize the effects of water stress during the tree growing season. After the model is calibrated and validated for all the species used in the study, we will use the model to predict the growth of the trees and their future response/acclimation to climate variability and change. For climate change analyzes, the Delta Change Method will be applied over several available CMIP5 GCM/RCM models (http://cmip-pcmdi.llnl.gov/) for two Representative Concentration Pathways (RCP). In order to analyze the potential range of the growth of trees under different projected levels of climate change, we will based our analysis on (a) RCP 8.5: Rising radiative forcing pathway leading to 8.5 W/m2 in 2100; and (b) RCP 4.5: Stabilization without overshoot pathway to 4.5 W/m2 at stabilization after 2100. For climate variability analyzes, the GeoSpatial Weather Generator (GiST) will be used to produce daily time series of maximum and minimum temperatures and precipitation based on the NOAA/CPC 3-month Outlooks Official Forecast for the region (http://www.cpc.ncep.noaa.gov/products/predictions/90day).

Progress 10/01/13 to 09/30/18

Outputs
Target Audience:Target audience included local agencies (e.g., USGS, DNR, NFS, NAFC, NGPC, Riparian Vegetation Task Force, Weed Management Association), natural resources managers, NE State Government representattives, participants at professional meetings, faculty and peers from inside and outside institutions, students, and professional community (international and national meetings and visits). Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Results were shared with professionals and natural resource managers. A total of 3graduate students and several undergraduate and high school students were trained and involved in the project.Additionally, 3postdoc, a technician and 3visiting scientists (from the Swiss Federal Institute, Mediterranean Agronomic Institute,and Chinese Academy of Science) were involved in the study which contributedto their professional development. Results from this study were shared with K-12 students through seminars and talks. Methods and findings were incorporated inplant ecophysiology and introduction to forest management courses. How have the results been disseminated to communities of interest?Results and findings from this project have been published in professional journals (8 peer reviewed manuscripts, 2 chapters in NE climate change reports, 1manuscriptunder review, and 2manuscripts under development),and shared in professional meetings and invited talks in the USA, China, Greece, Russia, and Switzerland. Results were shared with professionals and natural resource managers. A short video for science literacy was developed targeting K-12 with collaboration with NET TV. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? This research investigated the impacts of woody species invasion in NE semi-arid grasslands (eastern redcedar and ponderosa pine) and riparian areas (eastern redcedar and Russian olive) on ecosystemsfunctions with emphasis on ecohydrology, productivity, and diversity, and on determining environmental drivers, thresholds, and limitations to woody species expansion and growth under projected climate change. Specifically we used: - Quantitative ecophysiological and ecological approaches to study the impacts of ponderosa pine (Pinus ponderosa) and eastern redcedar (Juniperus virginiana)expansionin grasslands, and eastern redcedar and Russian olive (Eleagnus angustifolia)invasionin cottonwood (Populus deltoides) riparian forests on plant diversity and productivity, water balance, and resources availability. - Dendroecological approaches to determine past and present growth and responses of invasive and nativewoody speciestothe environmentand to stand density. - Dendroecological, ecophysiological and environmental data to forecast future woody species distribution as impacted by climate variability and change. To address the three objectives we established new sites and/or used long-term research sites (established by PIs) across precipitation gradient in NE (precipitation ranging from 850 to 570 mm in eastern and western NE, respectively). For detailed sites descriptionpleasesee Aus Der Au, et al., 2018; and Awada et al., 2013 and 2019.Since this is an integrated study, we highlight our findings in grasslands and riparian areas separately across all objectives. Grasslands study: We demonstrated in this 5 year-study that tree invasion in NE has led to a shift in vegetation composition, water availability, and soil characteristics, with significant plasticity of invasive woody species in response to resource availability (i.e. nutrients, light, and water) and limitation (e.g., Awada et al., 2013, Msanne et al., 2017). Eastern redcedar, together with other drought tolerant species in the ecosystem may play a dual role in the face of climate variability and change by either maintaining some ecosystem functioning in the face of prolonged periods of drought or impacting resilience due to the decline in biological and functional diversity in the system. We conclude thatunderstanding of the mechanisms of vegetation shift, physiological resistance strategies of species in response to abiotic and biotic stresses associated with climate variability and change, and thresholds in the face of climate extremes, are important for predicting the directional changes and invasion of these ecosystems in the face of climate change (e.g., Msanne et al., 2017, Aus der Au., 2018). Using Dendroecological (tree rings) and stable isotopes we conducted the first and most comprehensive study in NE (across precipitation gradient)on the drivers and thresholds of invasion, as well as impacts of projected climate variability on the expansion and performance of the two species. This study was conducted in collaboration with the Swiss Federal Institute for Forests, Snow and Landscapes (WSL) and the University of Zurich.We demonstratedthatspecies success in the drier western NE, washighly dependent on climate in the first years of establishment and on access to groundwater in the sampling and tree stage. Tree density was not a major factor. However in the eastern part of the state, the past, current and projected climate variability donot limit the expansion of invasive woody species especially that of eastern redcedar (MS Thesis, Aus der AU, 2017, Aus Der Au et al., 2018). Our preliminary observation also indicated that invasion is highest in private acreages and in unmanaged lands in eastern NE (still to be confirmed statistically). Finally, C13 data have shown that examined woody species have responded positively to increase in CO2 in the atmosphere in NE. Riparian study:we used dendroecological, ecophysiological and stable isotopes to demonstrate the differential acclimationof native Populus deltoides, invasive Elaeagnus angustifolia, and upland native aggressive Juniperus virginiana to streamflow fluctuations in a semi-arid riparian ecosystem along the Republican River. This has been a collaborative effort with WSL. Our results have shown that streamflow or water availability were the most important predictors of P. deltoides tree performance, and to a lesser extent J. virginiana. Elaeagnus angustifolia performance on the other hand was not significantly affected by environmental fluctuations, which increases the concern about the species and its future expansion. These results have significant ecological consequences along the Republican River and elsewhere. Altered ecohydrological processes have been shown to change riparian community composition, structure and function and, in many cases, facilitate the encroachment of native non-riparian or invasive alien species, further impacting the ecohydrology and surface water in the system. In fact, flood control and drought haveexasperated the problem by suppressing the regeneration of P. deltoides which relies on new mineral bed for germination and establishment, while facilitating the spread of J. virginiana and E. angustifolia, which once establish theythrive in the understory of P. deltoides. Based off current water flow management and without high flood pulses through the riparian zone, these species will continue to establish and spread throughout the Republican River watershed and elsewhere with significant impacts on the ecohydrology in the system (e.g., Awada et al., 2019).

Publications

  • Type: Journal Articles Status: Awaiting Publication Year Published: 2019 Citation: Awada, T., Skolaut, K., Battipaglia, G., Saurer, M., Riveros-Iregui, D., Schapaugh, A., Huddle J., Zhou, X., Martin, D., and Cherubini, P. (2019). Tree-ring stable isotopes show different ecophysiological strategies in native and invasive woody species of a semi-arid riparian ecosystem in the Great Plains of the USA. Ecohydrology. In Press.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Aus Der Au, R., Awada,T., Battipaglia, G., Hiller, J., Saurer, M., and Cherubini, P. (2018). Tree rings of Pinus ponderosa and Juniperus virginiana show different responses to stand density and water availability in the Nebraska Grasslands. American Midland Naturalist. 180(1):18-36.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2018 Citation: Awada, T. (2018). Phragmites invasion and ecosystem resilience, NWCA Spring Training, Ramada Inn, Kearney April 4, 2018.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2018 Citation: Awada, T. (2018). Tree-rings stable isotopes show different adaptive strategies of native and invasive woody species to streamflow fluctuations along the Republican River in NE. 13th Platte River Basin Ecosystem Symposium, June 5-6, Wood River, NE.
  • Type: Journal Articles Status: Submitted Year Published: 2019 Citation: Battipaglia G., Awada T., Aus der Au R., Sauer M., and Cherubini P. Increased CO2 facilitates the growth of Ponderosa pine into the semi-arid grasslands of Nebraska. submitted


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:Target audience included local agencies (e.g., USGS, DNR, NFS, NAFC, NGPC, Riparian Vegetation Task Force), natural resources managers, participants at professional meetings, faculty from inside and outside institutions, students, and professional community (international and national meetings and visits). Changes/Problems:Several of the participants on this project have left UNL: G. Baigorria, J. Msanne, S. Young, X. Zhou and C. Romero. I continue to collaborate with these faculty but the recent departure of Baigorria and Msanne have slowed down the modelling efforts, which may continue beyond the life of this project (2018 is the final year). What opportunities for training and professional development has the project provided?Results were shared with professionals and natural resource managers. Two graduate stduents completed their MS thesis, and one undergraduate students was trained. Additionally, a postdoc, a technician and 2 visiting scientists (from the Swiss Federal Institute and Chinese Academy of Science) are involved in the study which contributes to their professional development. Results from this study were shared with K-12 students through the delivery of a biosecurity and invasive species seminar. Methods and findings were incoporated in 2 independent study courses on plant ecophysiology. How have the results been disseminated to communities of interest?Results and findings from this project have been published in professional journals, and shared in professional meetings and invited talks (e.g., Biosecurity Seminar on Invasive Species, Chinese Academy of Science-Inner Mongolia, and TRACE conference in Russia). Results were shared with professionals and natural resource managers. A short video for science literacy was developed targeting K-12 with collaboration with NET TV. What do you plan to do during the next reporting period to accomplish the goals?- Continue the collection of long term data from permanent sites at the Nebraska National Forest at Halsey - Complete data analysis and manuscripts preparation. - Continue with our modelling efforts (final objective on this study). - Advise graduate and undergraduate students. - Disseminate results.

Impacts
What was accomplished under these goals? This research aims at investigating the impacts of woody species encroachment in semi-arid grasslands and riparian areas of NE on ecosystem functions and resilience with emphasis on ecohydrology, ecosystem productivity and diversity, and determining environmental drivers, thresholds, and limitations to woody species growth under projected climate change. We have used ecophysiological and dendroecological approaches to studying the impacts of ponderosa pine (Pinus ponderosa) and eastern redcedar (Juniperus virginiana) expansion in grasslands, and eastern redcedar and Russian olive (Eleagnus angustifolia) invasion into cottonwood (Populus deltoides) riparian forests on plant diversity and productivity, water balance, and resources availability. - Grasslands study: We demonstrated in this study that tree invasion in NE sandhills has led to a shift in vegetation composition, water availability, and soil characteristics, with significant plasticity of invasive woody species in response to resource availability (i.e. nutrients, light, and water) and limitation. Eastern redcedar together with other drought tolerant species in the ecosystem may play a dual role in the face of climate variability and change by either maintaining some ecosystem functioning in the face of prolonged periods of drought or impacting resilience due to the decline in biological and functional diversity in the system. We conclude that better understanding of the mechanisms of vegetation shift, physiological resistance strategies of species in grasslands in response to abiotic and biotic environmental stresses associated with climate variability and change, and thresholds in the face of climate extremes, are important for predicting the directional changes of these ecosystems in the face of climate change (Msanne et al., 2017). Using Dendroecological (tree rings) and stable isotopes to assess the performance of the ponderosa pine and eastern redcedar across a precipitation gradient in NE demonstrated that these two species success is highly dependent on groundwater access and precipitation in the drier central and western locations of the state, in contrast precipitation was not a limiting factor in the eastern locations (MS Thesis, Aus der AU, 2017). - In the riparian study, we used dendroecological, ecophysiological and stable isotopes to demonstrate the differential adaptation of native Populus deltoides, invasive Elaeagnus angustifolia, and upland native aggressive Juniperus virginiana to streamflow fluctuations in a semi-arid riparian ecosystem along the Republican River in the Great Plains, US. This has been a collaborative effort with the Swiss Federal Institute for Forests, Snow and Landscapes. Our results have shown that streamflow or water availability were the most important predictors of P. deltoides tree performance, and to a lesser extent J. virginiana. Elaeagnus angustifolia performance on the other hand was not significantly affected by the environmental fluctuations. These results have significant ecological consequences along the Republican River and elsewhere. Altered ecohydrological processes have been shown to change riparian community composition, structure and function and, in many cases, facilitate the encroachment of native non-riparian or invasive alien species, further impacting the ecohydrology and surface water in the system. Flood control in these areas has also exasperated the problem by suppressing the regeneration of P. deltoides which relies on new mineral bed for germination and establishment, while facilitating the spread of J. virginiana and E. angustifolia, two species that were widely planted across the Great Plains since 1900's, and are known to produce large number of seeds that can germinate under a range environmental conditions. Once established, both J. virginiana and E. angustifolia were found to thrive in the understory of P. deltoides and based off current water flow management and without high flood pulses through the riparian zone, these species will continue to establish and spread throughout the Republican River watershed and elsewhere with significant impacts on the ecohydrology in the system (Manuscript submitted).

Publications

  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Msanne, J., Awada, T., Bryan, N.M., Schacht, W., Drijber, R., Li, Y., Zhou, X., Okalebo J., Wedin, D., Brandle, J., and Hiller, J. (2017). Ecophysiological responses of native invasive woody Juniperus virginiana L. to resource availability and stand characteristics in the semi-arid grasslands of the Nebraska Sandhills. Photosynthetica, 55:219-230.
  • Type: Theses/Dissertations Status: Published Year Published: 2017 Citation: Aus Der Au, R. (2017). Two invasive species in the Sandhills of Nebraska: Responses to drought and water use efficiency. Department of Georgraphy, University of Zurich.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Awada, T. (2017). Invasive species and biosecurity - The Sandhills of Nebraska. Biosecurity Seminar, UNL, May 18.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Awada, T. (2017). Woody species encroachment in semi-arid grasslands with emphasis on Juniperus Virginiana. Chinese Academy of Sciences, Lanzhou, China. September 14.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Aus der Au, R., Awada, T., Egli, M., and Cherubini, P. (2017) Goodbye cowboy prairie! Tree Rings in Archaeology, Climatology and Ecology (TRACE) Conference, Svetlogorsk, Russia, May 16-21.


Progress 10/01/15 to 09/30/16

Outputs
Target Audience:Target audience included local agencies (e.g., USGS, DNR, NFS, NAFC, NGPC), natural resources managers, participants at professional meetings, faculty from inside and outside institutions, students, and professional community (international and national meetings and visits). Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Results were shared with professionals and natural resource managers. A short video for science literacy was developed with collaboration with NET TV targeting K-12 education. Two graduate and one undergraduate students are being trained and this study serves as the basis for their research. Additionally, a postdoc, a technician and a visiting scientist from the Swiss Federal Institute are involved in the study which contributes to their professional development. Results from this study were shared with an independent study course on plant ecophysiology. How have the results been disseminated to communities of interest?Two manuscripts were published, and one is in press. Results were shared with professionals and natural resource managers. A short video for science literacy was developed targeting K-12 with collaboration with NET TV. What do you plan to do during the next reporting period to accomplish the goals?- Complete dendroecological data collection - Complete analysis of ecophysiological measurements, and draft manuscripts - Initiate modelling efforts (final objective on this study) - Advise graduate and undergraduate students - Disseminate results with professionals and managers

Impacts
What was accomplished under these goals? Quantitative ecophysiological and ecological approaches to studying the impacts of ponderosa pine (Pinus ponderosa) and eastern redcedar (Juniperus virginiana) expansion in grasslands, and eastern redcedar and Russian olive (Eleagnus angustifolia) invasion into cottonwood (Populus deltoides) riparian forests on plant diversity and productivity, water balance, and resources availability. Data on tree water use using sapflux techniques in native and invasive woody species along riparian areas is being analyzed. We continue to collect data on environmental changes associated with increase in woody species in native grasslands. A manuscript on this topic was recently published. The article (Msanne et al., Photosynthetica) focuses on changes in environmental conditions (e.g., water, soil characteristics, temperature, humidity, etc.) as impacted by increase in woody species in grasslands and their feedback on tree function. The manuscript included findings on changes in photosynthetic capacity of Juniperus Virginiana that have not been published before especially in the most western distribution of this species. -Dendroecological approaches to determine past and present growth and responses of invasive and native woody species to the environment and to stand density. Cores collection from riparian sites has been completed and data analyzed, we are in the process of preparing a manuscript. Cores collection from upland sites were completed as well in the spring of 2016. Cores are being analyzed for standard dendroecological variables and for C and O isotopes. The team is collaborating with the Swiss Federal Institute of Forest, Snow and Landscapes on data analysis and students advising. -Dendroecological, ecophysiological and environmental data to forecast future woody species distribution as impacted by climate variability and change. This objective will be addressed in 2017 after the completion of dendroecological data collection and analysis, and the completion of ecophsyiological data collection. -Findings from the ecophysiological responses of invasive phragmites in riparian areas along the republican river were incorporated in the models that addressed changes in site evapotranspiration as impacted by phragmites invasion and control. A manuscript was published in Journal of Hydrology. Main findings have shown that control of phragmites along the republican river resulted in an average water saving of 245 mm (equivalent to 32% drop in site evapotranspiration).

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Mykleby, P.M., Lenters, J.D., Cutrell, G.J., Herrman, K.S., Istanbulluoglu, E., Scott, D.T., Twine, T.E., Kucharik, C.J., Awada, T., Soylu, M.E., and Dong, B. (2016). Energy and water response of a vegetated wetland to herbicide treatment of invasive Phragmites australis. Journal of Hydrology, 539:290-303.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2016 Citation: Msanne, J., Awada, T., Bryan, N.M., Schacht, W., Drijber, R., Li, Y., Zhou, X., Okalebo J., Wedin, D., Brandle, J., and Hiller, J. (2016). Ecophysiological responses of native invasive woody Juniperus virginiana L. to resource availability and stand characteristics in the semi-arid grasslands of the Nebraska Sandhills. Photosynthetica, early online access.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Nguy-Robertson, A., Buckley, E.M., Suyker, A.S., Awada, T. (2016). Determining factors that impact the calibration of consumer-grade digital cameras used for vegetation analysis. International Journal of Remote Sensing, 37:33653383.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Awada, T. (2016) Climate change and ne agroecosystems: emphasis on croplands and vegetation cover change in rangelands, Speaker and Panel Discussant, NLCV Conservation Summit, Creighton University, Omaha, NE, December 7.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Masis, T., Hiller, J., Morgan, P., Stoerger, V., and Awada, T. (2016). Application of high throughput plant phenotyping in natural resources management. UNL Plant Science Retreat. Lied Lodge and Conference Center, Nebraska City, NE.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Masis, T., Hiller, J., Morgan, P., Stoerger, V., and Awada, T. (2016). Application of high throughput plant phenotyping in natural resources management. AusPheno, 5th International Controlled Environmental Conference, Canberra, September 18-23.


Progress 10/01/14 to 09/30/15

Outputs
Target Audience:We communicated the goals of the project, as well as, previous and current findings with local agencies (e.g., Forest Service,DNR,NGPC), natural resources managers, state arboritum staff,scientists from inside and outside institutions, students, and professional community (e.g., national and international conferences). PIs participated in several conferences and professional meetings, and were invited to give seminars nationally and internationally (China, Switzerland and Greece). Research findings wereincoporated inclass lectures (e.g., Forest Ecology and Introduction to Forest Management). Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project provided support to forestry professionals and technicians, training to undergraduate and graduate students, and professional development to post-doctoral fellows. How have the results been disseminated to communities of interest?Results were shared through peer-reviewedpublications, personnal communications (PI shared data with colleagues),formal and informal discussion withprofessionals, stakeholders (e.g. round table discussion on climate change),students, natural resources managers and foresters. What do you plan to do during the next reporting period to accomplish the goals?1. Continue to collect data and process dendroecological samples. 2. Continue to collected soil (e.g., moisture, carbon, temperature, nutrients), and plant data (species composition, gas exchange, C13, nutrients., etc.). 3. Develop initial models to address objective 3 on species forecasting. 4. Submit manuscripts and present data at professional meetings. 5. Train undergraduate and graduate students. 6. Continue to support the development of post-doctoral fellows.

Impacts
What was accomplished under these goals? This research aims at investigating the impacts of woody species expansion in semi-arid grasslands and riparian areas of NE on ecosystem functions with emphasis on ecohydrology, and ecosystem productivity and diversity, and determining environmental drivers, thresholds, and limitations to woody species growth under projected climate change. Three objectives were identified, below is our progress to date (year 2 of the project) Quantitative ecophysiological and ecological approaches to study the impacts of ponderosa pine (Pinus ponderosa) andeastern redcedar (Juniperus virginiana) expansion in grasslands, and eastern redcedar and Russian olive (Eleagnusangustifolia) invasion in cottonwood (Populus deltoides) riparian forests on plant diversity and productivity, water balance, andresources availability. As part of our long term study on this topic, we have selected upland sites (open, mid and high density) at the Nebraska National Forest at Halsey and identified trees for measurements. Basic forest mensuration measurements and soil parameters were collected. Replicated soil cores were established at three depths at all sites in 2014, and measurements of soil gas carbon 12 and 13, soil temperature, and water content are being conducted bi-weekly during the growing season. Micrometeorological data were collected from a weather station. Leaf level gas exchange, C isotope, and water status measurements were conducted. On riparian site, three-year sap flux data collection has been completed (supported by a previous Mc.Stennis project funds), and data are being analyzed and manuscripts are in preparation. Dendroecological approaches to determine past and present growth and responses of invasive and native woodyspecies to the environment and to stand density. Cores collection from riparian sites has been completed and data analyzed, we will submit a manuscript in 2016. Cores collection from upland sites were completed in Fall of 2015. Cores are being analyzed for standard dendroecological variables and for C and O isotopes at co-investigator's institution in Zurich (WSL). Dendroecological, ecophysiological and environmental data to forecast future woody species distribution as impacted byclimate variability and change. This objective will be addressed in 2016 and 2017 as planned, after the completion of dendroecological data collection and analysis, and the completion of ecophsyiological data collection. Two new graduate students have joined our team in 2015, and one successfully graduated. PI is co-advising a student at the Swiss Federal Institute for Forests, Snow, and Landscape, who will assist with the dendroecology study. This student is fully supported by Swiss collaborators. Results on the method developmentfordetermination of growth habits in open and dense populations of J. virginiana (Zhou et al. 2015), have required that we develop biomass equation for the woody species we are investigating in an open setting which enables us to capture differences in growth (e.g., tree specific gravity and architecture). Data from destructively sampled open-grown trees were used to examine the uncertainties in the use of forest-derived equations for open-grown trees. Forest-derived equations provided good estimates of trunk biomass at lower diameter ranges but, as diameter increased, resulted in overestimation up to 40% for individual trees. Across the full diameter ranges, individual tree branch biomass was underestimated by 29-82%, depending on species and equation used. Although open-grown trunk and branch biomass curves diverged down and up, respectively, from their forest-derived counterparts, those for the whole tree tended to converge, albeit significantly above the forest-derived curves. Whole-tree biomass for individual trees was underestimated by at least 18%. To correct the biases, we studied the adjustment factor of forest- to open-grown tree biomass. It shows a power function with diameter. On a whole-tree basis, it was evaluated as a constant (1.2) independent of species and diameters. Application of this constant factor adjusted the biomass underestimation of three-species-mixed plantation by forest-derived equations from 21 to 4.6%, providing a cost-efficient approach to use forest-derived equations for open-grown trees in agriculture land. Results on the ecophysiology of introduced and native phragmites along riparian areas (Mykleby et al., 2015), showed that phragmites has the potential to modify habitats and lead to changes in ecosystem services. Our results have shown that native phragmites, with its higher rates of Amax (at 25-32°C), δ13C, water use efficiency (WUE) and photosynthetic nitrogen use efficiency (PNUE), has a better ability to deal with water fluctuations, water shortages, and warmer temperatures than its introduced counterpart, and could result in the native lineage being part of more diverse plant communities that can including woody species. In contrast, the faster growth rate, higher SLA, and lower WUE of the introduced lineage under cooler temperatures could result in greater carbon assimilation on a whole-plant level, giving introduced invasive phragmites an advantage and leading to further displacement of native communities in water-saturated habitats.

Publications

  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Zhou, X., Schoeneberger, M.M., Brandle, J., Awada, T., Chu, J., Martin, D.L., Li, J., Li, Y., and Mize, C.W. (2015). Analyzing the uncertainties in use of forest-derived biomass equations for open-grown trees in agricultural land. Forest Science, 61:144161.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Mykleby, P.M., Awada, T., Lenters, J., Bihmidine, S., Yarina, A. and Young, S. (2015). Ecophysiological responses of invasive and native phragmites australis to temperature and nitrogen fertilization. Great Plains Research. 25:63-74
  • Type: Other Status: Other Year Published: 2015 Citation: Awada, T. Woody Species Encroachment in a Semi-arid Grassland Ecosystem: the case of Juniperus virginiana. Naiman Desertification Research Station, Chinese Ecosystem Research Network, Cold and Arid Region Environmental and Engineering Research Institution, Chinese Academy of Sciences. September 7, 2015.(Invited Talk)
  • Type: Other Status: Other Year Published: 2015 Citation: Awada, T. (2015). Invasive woody species in a semi-arid grasslands in the Great Plains of the US: the case of Juniperus virginiana, The Birmensdorfer Tree-Ring Lectures, The Swiss Federal Institute for Forests, Snow and Landscapes, Zurich Switzerland, April 15, 2015.
  • Type: Journal Articles Status: Submitted Year Published: 2015 Citation: Msanne J., Awada, T., Bryan N.M, Schacht, W., Drijber, R., Li, Y., Wedin, W., Zhou, X., and Hiller, J. Environmental changes and feedback on tree ecophysiology associated with increased density of invasive woody species Juniperus virginiana in a semi-arid grassland ecosystem (Submitted).
  • Type: Journal Articles Status: Submitted Year Published: 2015 Citation: Mykleby, P.M., Lenters, J.D., Cutrell, G.J., Herrman, K.S., Istanbulluoglu, E., Scott, D.T., Twine, T.E., Kucharik, C.J., Awada, T., Soylu, M.E., and Dong, B. Water and energy balance response of a riparian wetland to herbicide treatment of invasive Phragmites australis (submitted).
  • Type: Journal Articles Status: Other Year Published: 2015 Citation: Nguy-Robertson, A., Buckleya, E.M., Suykera, A.S., Awada, T. Determining factors that impact the calibration of consumer-grade digital cameras used for vegetation analysis (Revise and resubmit)


Progress 10/01/13 to 09/30/14

Outputs
Target Audience: Target audience included local agencies (e.g., USGS, DNR, NFS, NAFC, NGPC), natural resources managers, faculty from inside and outside institutions, students, and professional community (e.g., international conferences, USA). Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? This project provided support to several forestry professionals, training to undergraduate and graduate students, and professional development to post-doctoral fellows. How have the results been disseminated to communities of interest? Two manuscripts are in press, results were shared with professionals, students, natural resources managersand foresters. What do you plan to do during the next reporting period to accomplish the goals? 1. Complete tree cores sampling, and initiate dendroecological analyses. 2. Continue to collected soil (e.g., moisture, carbon, temperature, nutrients), and plant data (species composition, gas exchange, C13, nutrients., etc.). 3. Develop initial models to address objective 3 on species forecasting. 4. Submit manuscritps and present data at professional meetings. 5. Train undergraduate and graduate students. 6. Continue to support the development of post-doctoral fellows.

Impacts
What was accomplished under these goals? This research aims at investigating the impacts of woody species invasion in semi-arid grasslands and riparian areas of NE on ecosystem functions with emphasis on ecohydrology, and ecosystem productivity and diversity, and determining environmental drivers, thresholds, and limitations to woody species growth under projected climate change. Three objectives were identified, below is our progress to date (year 1 of the project) -Quantitative ecophysiological and ecological approaches to study the impacts of ponderosa pine (Pinus ponderosa) and eastern redcedar (Juniperus virginiana)expansionin grasslands, and eastern redcedar and Russian olive (Eleagnus angustifolia)invasionin cottonwood (Populus deltoides) riparian forests on plant diversity and productivity, water balance, and resources availability.Six upland sites were selected with densities ranging from open grasslands to dense canopy eastern redcedar or ponderosa pine. Trees were identified and standard mensuration measurements were conducted on sites. Replicated soil coreswere established at three depths at all sites,and measurements ofsoil gas carbon 12 and 13, soil temperature,and water contentwere conducted onregular basis throughout the growing season.Micrometeorological datawere collected from a weather station.Leaf level gas exchange, C isotope,and water statusmeasurements were conducted. On riparian site, three-yearsap flux data collectionhas been completed (supported by a previous Mc.Stennisproject funds), and data arebeing analyzed. -Dendroecological approaches to determine past and present growth and responses of invasive and nativewoody speciestothe environmentand to stand density. Cores collection from riparian sites has been completed and data analyzed, we are in the process of preparing a manuscript. Cores collection from upland sites will be completed in spring 2015. Cores will be analayzed for standard dendroecological variables and for C and O isotopes. -Dendroecological, ecophysiological and environmental data to forecast future woody species distribution as impacted by climate variability and change. This objective will be addressed in 2016 and 2017 after the completion of dendroecological data collectionand analysis, and the completion of ecophsyiological data collection. Two manuscripts were completed and are in press, they will appear in 2015 (see publication list). Results were shared with professionals and natural resources managers.

Publications

  • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: 1. Zhou, X., Schoeneberger, M.M., Brandle, J., Awada, T., Chu, J., Martin, D.L., Li, J., Li, Y., and Mize, C.W. Analyzing the uncertainties in use of forest-derived biomass equations for open-grown trees in agricultural land. Forest Science. DOI: http://dx.doi.org/10.5849/forsci.13-071.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: Mykleby, P.m., Awada, T., Lenters, J.D., Bihmidine, S., Yarina, A.J., and Young, S.l., (2015). Ecophysiological responses of native and introduced genetic lineages of common reed (Phragmites australis) to nitrogen and temperature manipulations. Great Plains Research. In press.