ACQUISITION OF PULSED-FIELD GEL ELECTROPHORESIS EQUIPMENT

• Sponsoring Institution: National Institute of Food and Agriculture
• Project Status: COMPLETE
• Funding Source: AFRI COMPETITIVE GRANT
• Reporting Frequency: Annual
• Accession No.: 1000708
• Grant No.: 2013-67016-21179
• Cumulative Award Amt.: $15,631.00
• Proposal No.: 2013-01200
• Project Start Date: Sep 1, 2013
• Project End Date: Aug 31, 2014
• Grant Year: 2013
• Program Code: [A1221]- Animal Health and Production and Animal Products: Animal Health and Disease

Recipient Organization
OKLAHOMA STATE UNIVERSITY
(N/A)
STILLWATER,OK 74078

Performing Department
Veterinary Pathobiology

Non Technical Summary
We are seeking to expand and improve our research capabilities through acquisition of Pulsed-Field Gel Electrophoresis (PFGE) equipment. We will utilize this equipment to examine Mannheimia haemolytica and Pasteurella multocida isolates collected from the nasal passages, pharynx, trachea and bronchi in clinically normal and BRD-affected calves. This will permit determination of clonality of isolates throughout the respiratory tract. We will then characterize isolates collected from the upper respiratory tract of clinically normal calves from diverse sources, as well as isolates collected from the upper respiratory and lower respiratory tract of cattle suffering clinical BRD. Such robust characterization of isolates obtained from disparate anatomic locations, in healthy and ill calves, will enable us to determine if M. haemolytica and P. multocida behave as true opportunistic pathogens, or if a limited number of strains are most commonly associated with disease. If similar strains are found in multiple locations, and in both healthy and affected calves, the bacteria are likely true opportunists. If greater diversity is seen in the upper respiratory tract than in the lungs of diseased cattle, it is likely that certain strains have greater virulence. These studies can assist in determining whether greater attention should be devoted to highly pathogenic strains of M. haemolytica and P. multocida, or whether research efforts are best directed at bolstering host immune defenses.

Animal Health Component

(N/A)

Research Effort Categories

Basic

100%

Applied

(N/A)

Developmental

(N/A)

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
311	3399	1100	100%

Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3399 - Beef cattle, general/other;

Field Of Science
1100 - Bacteriology;

Keywords

pulsed-field gel electrophoresis

pfge

molecular epidemiology

bovine respiratory disease

brd

Goals / Objectives
Acquire pulsed-field gel electrophoresis equipment to extend our molecular epidemiology capabilities.

Project Methods
Equipment will be used in a variety of projects examining epidimologic relationships among various bacterial agents.

Progress 09/01/13 to 08/31/14

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? The sole objective was accomplished with the purchase of the Bio-Rad CHEF Mapper XA System purchased in October 2013. The equipment is now set up in the lab and will be used for future research projects, funding support for which has already been sought.

Publications