Progress 01/01/14 to 12/31/18
Outputs
Target Audience:Scientists, professionals, and technical personals in the food safety research community Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project has trained three graduate students and three undergraduate students during the past year. Two graduate students and two undergraduate students at North Carolina Central University have been trained in the microbiology field, focusing on the microbiological methods for evaluation of the efficiency of CNTs coated filters for removing pathogens in solutions. One graduate student and one undergraduate student at Clemson University were trained in the field of synthesis, modification, and characterization of CNTs, functionalized CNTs, and the preliminary work on nano-sensors. These students have benefited significantly from their participating in the project. The undergraduate students are looking to pursue their graduate education in relevant STEM fields. How have the results been disseminated to communities of interest?Two conference presentations, and one manuscript ready to be submitted to the research communities of interest for this report period: Liju Yang Liju Yang. 2018. Antimicrobial Carbon-Based Nanomaterials. Oral Presentation, AiMES 2018 Meeting, Sept. 30 - Oct. 4, 2018, Cancun, Mexico. Xiuli Dong, Ambrose E. Bond, and Liju Yang. Highly Efficient Antimicrobial Function of Essential Oil-Incorporated Carbon Nanotubes Coated Filters. In Preparation for submission. Ya-Ping Sun. Novel Carbon Nanomaterials for Food Safety and Health Applications. 10th International Conference on Food Engineering and Biotechnology, March 26-29, 2019, Tokyo, Japan. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Over the last year of the project, our research teams have been working collaboratively across all three specific aims of the project to conclude the tasks already in progress and also to look into issues and opportunities that we have learned from the project effort in preparation for the further development of the technology with future USDA support and other resources. We have focused on the incorporation of essential oils (EOs) to CNTs coatings on the filters, testing of new protocols for effective coating of filters, the evaluation of these EOs-CNTs-coated filters for the removal and inactivation of bacterial cells from aqueous solutions, and the challenges and potentially effective solutions for the needs in rapid and ultrasensitive detections down to a few bacterial cells by coupling the isolation-concentration technology with the emerging ultra-bright fluorescence nano-sensors. Essential oils might be a good choice as an effective coating for the development of antimicrobial filters, since they are safe and beneficial to the human health, and they are widely used in the cosmetic, food, perfume, and pharmaceutical industries. Particularly interesting and valuable are their antimicrobial effects. Therefore, we investigated to couple EOs with CNTs for more efficient dual functions in concentrating and inactivating bacterial pathogens. We continued the evaluation of the coating of the commercially available filters with the EOs-functionalized CNTs. We found that lemon essential oil (LO) and tea tree oil (TTO) exhibit significant inhibitory effects against bacteria. We developed highly effective antimicrobial MWCNTs filters with TTO or LO coatings, especially TTO-MWCNTs filters, which could immediately and completely inactivate (100%) captured E.coli bacteria and also remove 100% bacterial cells in PBS. However, these newly developed filters have not been tested on real food samples. It will require additional time and effort to test them on food samples. By taking advantage of our understanding acquired from our project effort and for the consideration of eventually implementing the technology for isolation-concentration of foodborne pathogens in food safety applications, we did some explorations on the potential coupling of the technology with rapid and ultrasensitive detection methods, mostly for the purpose of assessing and understanding fundamental and technical feasibilities and some potential limitations. A most attractive approach thus identified is to incorporate nano-sensors into the filtration protocol to enable rapid and ultrasensitive detections of the isolated bacterial cells. The functionalized CNTs used in the isolation-concentration were found to exhibit significant fluorescence emissions, thus capable of the desired sensor functions in situ. Beyond that a more robust approach is to couple with the ultra-bright fluorescence probes based on carbon quantum dots developed in our groups, which share the same mechanistic origins as the observed fluorescence emissions in the functionalized CNTs. Feasibility analyses based on available experimental results suggest that the coupled filter-sensor method will enable to achieve substantially lower detection limits from those in the current state of the art.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2018
Citation:
Liju Yang. 2018. Antimicrobial Carbon-Based Nanomaterials. Oral Presentation, AiMES 2018 Meeting, Sept. 30 Oct. 4, 2018, Cancun, Mexico
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2019
Citation:
Ya-Ping Sun. Novel Carbon Nanomaterials for Food Safety and Health Applications. 10th International Conference on Food Engineering and Biotechnology, March 26-29, 2019, Tokyo, Japan
- Type:
Journal Articles
Status:
Submitted
Year Published:
2019
Citation:
Xiuli Dong, Ambrose E. Bond, and Liju Yang. Highly Efficient Antimicrobial Function of Essential Oil-Incorporated Carbon Nanotubes Coated Filters. Submitted.
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Progress 01/01/17 to 12/31/17
Outputs
Target Audience:Scientists,researchers, and studentsin the food safety and antimicrbial fields. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project has trained two graduate students and two undergraduate students during the past year. Two graduate students and one undergraduate student at North Carolina Central University have been trained in the microbiology field, focusing on the microbiological methods for evaluation of the efficiency of CNTs coated filters for removing pathogens in solutions. One undergraduate student at Clemson University has been trained in the field of synthesis, modification, and characterization of CNTs, functionalized CNTs, and the magnetic nanoparticles. These students have benefited significantly from their participating in the project, and undergraduate students are looking to pursue their graduate education in relevant STEM fields. How have the results been disseminated to communities of interest?Two conference presentations and one manuscript have been disseminated to the research communities of interest during this report period: Liju Yang. 2017. Functionalized Carbon Nanotubes-Coated Filters For Bacterial Isolation/Concentrating. Oral Presentation, The 2017 Annual Meeting of Institute of Biological Engineering (IBE), Salt Lake City, UT, March 30-April 1, 2017. Ya-Ping Sun. 2017. Carbon Nanomaterials of Different Dimensions: From Sheets to Dots, and from Energy Conversion to Bioimaging and Bactericidal Functions. Oral Presentation, 2017 MRS Fall Meeting & Exhibit - Materials Research Society, Boston, MA, November 26-December 1, 2017. Xiuli Dong1, Mohamad Al Awak1, Ping Wang,2 Ya-Ping Sun2,*, and Liju Yang1,* ?2017.Carbon Dots Incorporated Multi-walled Carbon Nanotube Coated Filters for Bacterial Removal and Inactivation. Submitted to J. Mater. Chem. C What do you plan to do during the next reporting period to accomplish the goals?In the next project period, we will keep moving forward in the project and conduct the experiments as proposed. We will continue the optimization of different modified CNTs, mainly including those with different carbohydrate residues and magnetic CNTs, for coating on the filters, and investigate the efficiency of these coated filters for removing and concentrating bacterial pathogens. Testing on the best performed CNTs coated filters on different food samples will be another focus of the project. Meanwhile, we will continue to train our graduate and undergraduate students in the food safety research areas.
Impacts
What was accomplished under these goals?
Our research teams have been collaboratively working across all three specific aims of the project over the past year. We focused on the synthesis and functionalization of CNTs, the design and testing of new protocols for effective coating of filters, and the evaluation of these CNTs-coated filters for the removal and inactivation of bacterial cells from aqueous solutions. For specific aim 1, we continued our effort on incorporating selected carbohydrates, including chitosan and mannan (a highly branched polymer of mannose), into the PPEI functionalization of CNTs. These carbohydrate-PPEI co-functionalized CNTs were designed to exploit specific interactions of the carbohydrates with bacterial cells, which were known from previously unrelated projects. We also pursued the synthesis of magnetic CNTs in different approaches. One more successful approach was to synthesize functionalized small carbon nanoparticles embedded or attached with magnetic iron oxide (F3O4) nanoparticles as carriers of the magnetic properties for the subsequent coupling with CNTs via the same functional groups. In addition, the nanoparticles could be clustered for larger particles of much stronger magnetic responses for their being used either directly or coupled with CNTs for more efficient magnetic separation or concentration of bacterial cells. For specific aim 2, we continued the evaluation of different methods for the coating of the commercially available filters with the above functionalized CNTs. We found that the "locking" of CNT coating on the filter is critical to prevent any re-suspension of the CNTs during the bacteria-capturing experiments, and such coating strategy resulted in an even distribution of the CNTs and improved the bacterial capture efficiency during filtration. Particularly, we found a method to incorporate the newly discovered photo-activated antimicrobial carbon dots (CDots) onto the MWCNTs coating on the filters, by which the coated filters were afforded with simultaneous dual functions of capturing bacterial cells and inactivating them. A manuscript on this technique has been submitted to peer- reviewed journal for publication. With the magnetic iron oxide (Fe3O4) nanoparticles that are partially carbon-coated and also with organic functional molecules on the surface, as discussed in aim 1 above, we are looking at possibly their decoration of CNT-coated filters for the capturing of bacterial cells. For specific aim 3, we continued our effort on the evaluation of the efficiency of the newly synthesized CNTs- and their derivatives-coated filters for removal of bacterial cells for Gram negative E. coli K12 and Gram positive Bacillus cells. Next year, we will be focusing on the testing of those best-performing CNTs (or incorporated with other nano components) coated filters for removal of bacterial pathogens from bacterial inoculated food samples
Publications
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2017
Citation:
Liju Yang. 2017. Functionalized Carbon Nanotubes-coated Filters for Bacterial Isolation/Concentrating, Oral Presentation, The 2017 Annual Meeting of Institute of Biological Engineering (IBE), Salt Lake City, UT, March 30-April 1, 2017
- Type:
Journal Articles
Status:
Submitted
Year Published:
2018
Citation:
Xiuli Dong1, Mohamad Al Awak1, Ping Wang,2 Ya-Ping Sun2,*, and Liju Yang1,*Carbon Dots Incorporated Multi-walled carbon nanotube coated filters for bacterial removal and inactivation. Submitted to J. Mater. Chem.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2017
Citation:
Ya-Ping Sun. 2017. Carbon Nanomaterials of Different Dimensions: From Sheets to Dots, and from Energy Conversion to Bioimaging and Bactericidal Functions. Oral Presentation, 2017 MRS Fall Meeting & Exhibit - Materials Research Society, Boston, MA, November 26-December 1, 2017
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Progress 01/01/16 to 12/31/16
Outputs
Target Audience:Scientists, professionals, and technical personnels in the food safety research community. Changes/Problems:Due to the time needed to recruit new graduate students to fill in the research assitant position of graduated studentson this project, it is likely that we will need to request anone-year no-cost extension for the project. What opportunities for training and professional development has the project provided?The project has trained three graduate students and three undergraduate students during the past year. One graduate student at Clemson University has been trained in the field of synthesis, modification, and characterization of CNTs and derivatives. Two graduate students at North Carolina Central University have been trained in the microbiology field, focusing on the microbiological methods for evaluation of the efficiency of CNTs samples coated filters for removing pathogens in solutions. One of them graduated in Aug, 2016. One undergraduate student at NCCU was just recruited to this project, and currently trained with very basic laboratory techniques. The two summer students at Clemson benefited significantly from their participating in the project, and both of them are looking to pursue their graduate education in relevant STEM fields. How have the results been disseminated to communities of interest?One MS thesis and one national/international conference poster presentation have been published during this report period: Shengyuan Wang, 2016. Investigation of Polymer-functionalized Carbon Nanotube-Coated Filters for Removal of Bacteria, NCCU Master's Thesis. Shengyuan Wang, Xiuli Dong, Gregory E. LeCroy, Fan Yang, Ya-Ping Sun, Liju Yang, Investigation of Polymer-functionalized Carbon Nanotube-Coated Filters for Removal of Bacterial Pathogens. 2016 Annual Meeting of the Institute of Biological Engineering (IBE),April 7-9, 2016, Greenville, SC What do you plan to do during the next reporting period to accomplish the goals?In the next project period, we will move forward the project and conduct the experiments as we proposed. We will continue the design and synthesis of different modified CNTs, mainly including those with different carbohydrate residues and magnetic CNTs, and investigate the efficiency of these functionalized CNTs coated filters for removing and concentrating bacterial pathogens, and testing with bacterial inoculated food samples.
Impacts
What was accomplished under these goals?
Our research teams have collaboratively worked across all three specific aims of the project over the past year. We focused on the synthesis and functionalization of CNTs with different molecules, the design and testing of new protocols for coating filters with the specifically functionalized CNTs, and the evaluation of these CNTs-coated filters for the removal of bacterial cells from solutions. For specific aim 1, first, we have continued our effort on the synthesis in sufficient quantities of a series of PPEI-CNTs and PPEI-EI-CNTs, where PPEI refers to the aminopolymer polypropionylethyleneimine (molecular weights 5,000 and 50,000) and PPEI-EI to the copolymer poly(propionylethyleneimine-co-ethyleneimine) (from the hydrolysis of PPEI). These functionalized CNTs were used to coat commercially acquired filters of different pore sizes for a further confirmation of their abilities to capture bacteria from solutions and for evaluations aimed at improvements toward optimization of the bacterial removal efficiency. We have also pursued the introduction of selected carbohydrates, chitosan and mannan (a highly branched polymer of mannose) in particular, into the PPEI functionalization of CNTs. These carbohydrate-PPEI co-functionalized CNTs were designed to exploit specific interactions of the carbohydrates with bacterial cells. For specific aim 2, we have continued the evaluation of various methods for the coating of the commercially available filters of different pore sizes with the functionalized CNTs, including those containing the carbohydrates. We found that the coating outcomes, such as the distribution of the CNTs and the morphology of the coating on the filters, are important to the capturing of bacteria from solutions. We have also explored options to "lock" the coating to prevent any re-suspension of the CNTs during the bacteria-capturing experiments. We have prepared magnetic iron oxide nanoparticles (Fe3O4) that are partially carbon-coated and also with organic functional molecules on the surface for controlling the particle sizes and also the dispersion of the overall nanoparticles. These coated and functionalized magnetic nanoparticles are designed to be used in the decoration of CNTs to impart magnetic properties for the capturing of bacterial cells. Major milestones in the preparation and characterization of the magnetic nanoparticles have been achieved, and their introduction in the functionalization of CNTs for magnetically responsive functionalized CNTs is planned. For specific aim 3, we have continued our effort on the evaluation of the efficiency of the newly synthesized CNTs and derivatives coated filters for removal of bacterial cells for gram negative E. coli K12 and Gram positive Bacillus cells. We are planning to move onto the testing of bacterial inoculated food samples.
Publications
- Type:
Theses/Dissertations
Status:
Submitted
Year Published:
2016
Citation:
Shengyuan Wang, 2016. Investigation of Polymer-functionalized Carbon Nanotube-Coated Filters for Removal of Bacteria, NCCU Master's Thesis.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2016
Citation:
Shengyuan Wang, Xiuli Dong, Gregory E. LeCroy, Fan Yang, Ya-Ping Sun, Liju Yang,
Investigation of Polymer-functionalized Carbon Nanotube-Coated Filters for Removal of Bacterial Pathogens. 2016 Annual Meeting of the Institute of Biological Engineering (IBE), Greenville, SC, April 7-9, 2016.
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Progress 01/01/15 to 12/31/15
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project has recruited and trained two graduate students. One graduate student at Clemson University has been trained in the field of synthesis, modification, and characterization of CNTs and derivatives. One graduate student at North Carolina Central University has been trained in the microbiology field, focusing on the microbiological methods for evaluation of the efficiency of CNTs samples coated filters for removing pathogens in solutions. In addition, one postdoc research associate at NCCU worked partially on this project, gaining research experience in the interface of microbiology and nanotechnology, as well as getting experience in mentoring student by assisting the PI to supervisethe graduate student in lab. How have the results been disseminated to communities of interest?Two papers have been published during this report period: Shengyuan Wanga, Gregory E. LeCroyb, Fan Yangb, Xiuli Donga, Ya-Ping Sunb† , Liju Yanga†. 2015. Carbon Nanotube-Assisted Capturing of Bacterial Pathogens. RSC Advances, 5, 91246-91253 Xiuli Dong, Liju Yang*. 2015. Dual Functional Nisin-Multi-walled Carbon Nanotubes Coated Filters for Bacterial Capture and Inactivation. Journal Biological Engineering, 9:20 What do you plan to do during the next reporting period to accomplish the goals?In the next report period, we will move forward the project and conduct the experiments as we proposed. We will continue the design and synthesis of different modified CNTs, including those with carbohydrate residues and magnetic CNTs, and investigate the efficiency of these functionalized CNTs coated filters for removing and concentrating bacterial pathogens. Factors to be investigated include different functionalization moieties, different filter coating densities, and different species of bacteria for evaluations. Meanwhile, we will be looking into something new functionalized CNTs, including working on the use of graphene oxides (GOs) to non-covalently functionalize (more like dispersing) MWNTs, or GOs-MWNTs, for coating hydrophilic filters, and finding polymers and other functionalization agents that are more compatible with the filter materials, including how to incorporate sugars into the functionalization.
Impacts
What was accomplished under these goals?
We at both NCCU and CU have worked across all three specific aims and established protocols for synthesis and functionalization of CNTs and derivatives, coating filters with synthesized CNTs and their derivatives, and evaluating CNTs-coated filters for the removal of bacterial cells from solutions. For specific aim 1, we have synthesized two sets of CNT samples for testing their function of capturing bacterial cells. One set of samples varied with processing methods, in which we used thermal, microwave, and chemical synthesis to synthesize PPEI-EI-CNTs. The other set of samples varied with three configurations of surface functionalization, including the PPEI-EI-functionalized CNTs, PPEI/PEI-co-functionalized CNTs, and Mannose/PPEI-EI-co-functionalized CNTs. The CNTs used so far were multiple-walled (MWNTs). PPEI-EI refers to an inexpensive co-polymer poly(propionylethyleneimine-co-ethyleneimine, with two average molecular weights of 5,000 (used primarily) and 50,000 (for comparison). The functionalized CNT samples were used in the coating of filters for the evaluation of their trapping bacterial cells. For specific aim 2, To select appropriate filters for coating, different types of filters have been tested and evaluated including the hydrophobic PTFE filters and the hydrophilic polycarbonate filters (from Millipore), both types with pore sizes of 1.2 um and 3.0 um. The results indicated that hydrophilic polycarbonate filters were better for PPEI-EI-MWNTs coating, with more reproducible results, and hydrophobic PTFE filters were not as good for the coating as flected by the poor reproducibility in results. For specific aim 3, we have established a protocol to evaluate the efficiency of CNTs and derivatives coated filters for removal of bacterial cells using Gram negative E. coli K12 and Gram positive Bacillus cells. We have determined and compared the removal efficiency of different CNTs samples and at different conditions, and a summary of the results is as follows: For the samples with different processing methods, the chemical synthesized samples showed their effective function in capturing E. coli cells, and the capture efficiency was dependent on the coating density. However, the samples with microwave and thermal processing did not show capture function for bacterial cells. For the samples with different surface functionalizations, our results showed that PPEI-EI-CNTs were the most effective in capturing E. coli cells when coating on 1.2 um polycarbonate filters (> 4 log). PPEI/PEI-co-functionalized CNTs showed less efficiency in capturing E. coli cells (~2 log); and Mannose/PPEI-EI-co-functionalized CNTs showed the least capture efficiency (< 1 log). We have also tested the samples with different surface functionalizations for Gram positive bacterial Bacillus anthracis cells on 3.0 um filters, and the results showed the same trend as that for capturing Gram negative E. coli cells. PPEI-EI-CNTs coated filters had a 3 log reduction, PPEI/PEI-co-functionalized CNT-coated filters had ~2 log reduction, while mannose/PPEI-EI-co-functionalized CNTs-coated filters had < 1 log reduction. Mechanism-related study is in progress.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Shengyuan Wanga, Gregory E. LeCroyb, Fan Yangb, Xiuli Donga, Ya-Ping Sunb , Liju Yanga . 2015. Carbon Nanotube-Assisted Capturing of Bacterial Pathogens. RSC Advances, 5, 91246-91253.
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Xiuli Dong, Liju Yang*. 2015. Dual Functional Nisin-Multi-walled Carbon Nanotubes Coated Filters for Bacterial Capture and Inactivation. Journal Biological Engineering, 9:20
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Progress 01/01/14 to 12/31/14
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? The project has recruited and trained two graduate students. One graduate student at Clemson University has been trained in the field of synthesis, modification, and characterization of CNTs and derivatives. One graduate student at North Carolina Central University has been trained in the microbiology field, focusing on the microbiological methods for evaluation of the efficiency of CNTs samples coated filters for removing pathogens in solutions. How have the results been disseminated to communities of interest? We disseminate the results of the project to the communities of inerestthrough conference presentation and journal article publications, the following items are either published or in progress for publishing: One paper acknowledging the USDA funding was published: "Carbon-Based Quantum Dots for Fluorescence Imaging of Cells and Tissues?, authored by Luo, P. G.; Sonkar, S. K.; Yang, S.-T.; Yang, F.; Yang, L.; Broglie, J. J.; Sun, Y.-P. RSC Adv. 2014, 4, 10791-10807. One abstract was accepted by the 2015 Institute of Biological Engineering (IBE) Annual Meeting for poster presentation. Due to the cancellation of flight in inclement weather, the trip to the IBE meeting was cancelled. The Poster was: Investigation of Polymer-functionalized Carbon Nanotube-Coated Filters for Removal of Bacterial Pathogens, authored by Shengyuan Wang1, Xiuli Dong1, Gregory E. LeCroy2, Fan Yang2, Ya-Ping Sun2, Liju Yang1* A manuscript partially supported by the grant was submitted to Journal of Bionanotechnology (BioMed Central), and currently in review. Title: Superior Antibacterial Activity of Photochemical Synthesized Silver Nanoparticles-Carbon Nanotubes Nanocomposites and Their Synergistic Effects in Combination with Other Antimicrobial Agents, authored by Xiuli Dong1, Youngmi Koo2, Yongan Tang3, Yeoheung Yun2, Liju Yang1* A manuscript from this project is in preparation, Title: Polymer-functionalized Carbon Nanotube-Coated Filters for Removal of Bacterial Pathogens, authored by Shengyuan Wang1, Xiuli Dong1, Gregory E. LeCroy2, Fan Yang2, Ya-Ping Sun2, Liju Yang1 What do you plan to do during the next reporting period to accomplish the goals? In the next report period, we will move forward the project and conduct the experiments as we proposed. We will continue the design and synthesis of different modified CNTs, including those with carbohydrate residues, and investigate the efficiency of these functionalized CNTs coated filters for removing and concentrating bacterial pathogens. Factors to be investigated include different functionalization moieties (carbohydrates, for example), different filter coating densities, and different species of bacteria for evaluations. In addition, we will initiate the synthesis of magnetic CNTs and their functionalization for concentrating of pathogens in suspensions.
Impacts
What was accomplished under these goals?
We at both NCCU and CU have worked across all three specific aims and established protocols for synthesis and functionalization of CNTs and derivatives, coating filters with synthesized CNTs and theirs derivatives, and evaluating CNTs-coated filters for the removal of bacterial cells from solutions. For specific aim 1, we have designed and synthesized several configurations of the PPEI-EI-functionalized CNTs, where PPEI-EI refers to an inexpensive co-polymer poly(propionylethyleneimine-co-ethyleneimine and the CNTs used so far were multiple-walled (MWNTs). The variations in these samples included PPEI-EI of two different molecular weights (5,000 and 50,000), different functionalization chemistries and processing conditions (amidation and thermochemical methods), and different post-functionalization sample selections (gravimetric fractionation and cross-linking to yield samples containing networked species for more filter-coating options). The samples were used in the coating of filters for the evaluation of their trapping bacterial cells. For specific aim 2, we have used the synthesized PPEI-EI-MWNTs to coat filters. To select appropriate filters for coating, different types of filters have been tested and evaluated including the hydrophobic PTFE filters and the hydrophilic polycarbonate filters (from Millipore), both types with pore sizes of 1.2 um and 3.0 um. The results indicated hydrophilic polycarbonate filters were appropriate for PPEI-EI-MWNTs coating and generated reproducible results, however, hydrophobic PTFE filters were not appropriate for the coating due to not being able to generate reproducible results. For specific aim 3, we have established a protocol to evaluate the efficiency of CNTs and derivatives coated filters for removal of bacterial cells using E. coli K12. We have determined and compared the removal efficiency of different CNTs samples and at different conditions, including the following: the efficiency of PPEI-EI (5,000)-MWNTs and PPEI-EI (50,000)-MWNTs for removal of E. coli cells. The results indicated PPEI-EI (5,000)-MWNTs coated filters were better in removing bacterial cells. the efficiency of PPEI-EI (5,000)-MWNTs at different coating densities on polycarbonate filters. The results indicated that the removal efficiency increased with the coating density ranging from 0.094 ug/mm2 to 0.73 ug/mm2, and reached 5 log viable cell reduction at optimal condition. The efficiencies of above filters at different flow rates, 0.25 ml/min and 0.5 ml/min were determined. The low flow rate generated a better removal efficiency. The efficiency of cross-linked PPEI-EI-MWNTs coated filters (polycarbonate filters). The results indicated that the cross-linked PPEI-EI-MWNTs coating also exhibited efficient removal of bacteria, achieved approximately 2 log in viable cell reduction. No obvious coating density dependency was observed, most likely due to the uneven distribution of the nanotube sample on the filters. The distribution and morphology of CNTs samples coated on the filters were examined by scanning electron microscopy.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Carbon-Based Quantum Dots for Fluorescence Imaging of Cells and Tissues?, authored by Luo, P. G.; Sonkar, S. K.; Yang, S.-T.; Yang, F.; Yang, L.; Broglie, J. J.; Sun, Y.-P. RSC Adv. 2014, 4, 10791-10807
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2014
Citation:
2015 Institute of Biological Engineering (IBE) Annual Meeting for poster presentation. The Poster was: Investigation of Polymer-functionalized Carbon Nanotube-Coated Filters for Removal of Bacterial Pathogens, authored by Shengyuan Wang1, Xiuli Dong1, Gregory E. LeCroy2, Fan Yang2, Ya-Ping Sun2, Liju Yang1*. Due to the cancellation of flight in inclement weather, the trip to the IBE meeting was cancelled.
- Type:
Journal Articles
Status:
Under Review
Year Published:
2015
Citation:
Superior Antibacterial Activity of Photochemical Synthesized Silver Nanoparticles-Carbon Nanotubes Nanocomposites and Their Synergistic Effects in Combination with Other Antimicrobial Agents, authored by Xiuli Dong1, Youngmi Koo2, Yongan Tang3, Yeoheung Yun2, Liju Yang1*, under review, Journal of Bionanotechnology (BioMed Central)
- Type:
Journal Articles
Status:
Other
Year Published:
2015
Citation:
Polymer-functionalized Carbon Nanotube-Coated Filters for Removal of Bacterial Pathogens, authored by Shengyuan Wang1, Xiuli Dong1, Gregory E. LeCroy2, Fan Yang2, Ya-Ping Sun2, Liju Yang1. In preparation.
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