Source: OREGON STATE UNIVERSITY submitted to NRP
USE OF MELOXICAM TO ALLEVIATE THE TRANSPORT-ELICITED ACUTE-PHASE REACTION IN FEEDER CATTLE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1000281
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 24, 2013
Project End Date
Sep 30, 2013
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
OREGON STATE UNIVERSITY
(N/A)
CORVALLIS,OR 97331
Performing Department
Eastern Oregon Agricultural Res Center
Non Technical Summary
The stressors associated with the transfer of beef calves from the ranch of origin to commercial feedlots are major predisposing causes for bovine respiratory disease (BRD), an infectious complex that costs the US cattle industry approximately $ 1 billion/year. Road transport and feedlot entry, considered major stressors during the aforementioned instance, have been shown to elicit inflammatory and acute-phase responses in overtly healthy cattle. These stress-induced immune responses are highly demanding and detrimental to subsequent cattle performance and health by increasing the BRD incidence. Therefore, alternatives to modulate immune reactions elicited by stressful but routine management procedures are warranted to promote animal welfare and productivity. Our group demonstrated that administration of anti-inflammatory compounds to cattle prior to and after transport effectively alleviated the acute-phase response during feedlot receiving. More specifically, supplementation with linolenic acid prior to transport and during feedlot receiving reduced the acute-phase protein response but did not benefit feedlot receiving performance. Administration of Flunixin Meglumine, a non-steroidal anti-inflammatory drug (NSAID), at truck loading/unloading also alleviated the acute-phase protein response but did not improve feedlot receiving performance. Perhaps the elimination half-life of flunixin meglumine (less than 8 h) was insufficient to modulate the transport-elicited acute-phase response to an extent that resulted in enhanced cattle performance. Alternatively, meloxicam has an elimination half-life of 28 h when orally administered to cattle at 1 mg/kg. Accordingly, Van Engen et al. (2014) reported that oral administration of meloxicam to cattle prior to a 16-h road transport reduced transport-induced inflammatory reactions, although authors did not evaluate feedlot receiving performance. Based on this rationale, we hypothesized that oral meloxicam administration prior to transport and during feedlot receiving alleviates the acute-phase response and improves performance of feeder cattle. Hence, the objective of this experiment was to evaluate the effects of oral meloxicam administration on circulating concentrations of cortisol, NEFA, acute-phase proteins, and feedlot receiving performance of transported cattle.
Animal Health Component
50%
Research Effort Categories
Basic
20%
Applied
50%
Developmental
30%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3053310102050%
3113310109050%
Goals / Objectives
Evaluate the impacts of meloxicam administration prior to transport and during feedlot receiving on the acute-phase and performance parameters of feeder cattle
Project Methods
Eighty-four steers weaned at 7 months of age (day -30) and vaccinated against common infectious diseases including BRD complex, will be ranked by body weight and age on day 0 of the study, and assigned to 21 feedlot pens (4 steers/pen). Pens will be randomly assigned to 1 of 3 treatments: 1) continuous road transport for 900 miles and daily administration of Meloxicam (1 mg/kg of BW daily) from day 0 to day 7, 2) continuous road transport for 900 miles and daily administration of lactose monohydrate (1 mg/kg of BW daily; a pharmacologically inactive excipient used in the manufacture of Meloxicam tablets) from day 0 to day 7, or 3) no transport and daily administration of lactose monohydrate (1 mg/kg of BW daily) from day 0 to day 7. The Meloxicam dose adopted herein is based on the oral administration utilized by Coetzee et al. (2010 / Journal of Animal Science 90:1026-1039) and Repenning et al. (2013 / J. Anim. Sci. 91: 4965-4974) to weaned beef cattle (1 mg/kg of BW daily). Further, Meloxicam will be administered to assigned steers on day 0 via oral drench (mixed with water) to modulate stress-induced inflammatory reactions elicited during transport. From day 1 to day 7 of feedlot receiving, Meloxicam will be mixed in the dietary concentrate and is expected to modulate residual as well as novel stress reactions elicited by unloading and feedlot entry.Beginning on day 1, all pens will be offered a feedlot receiving diet (50:50 forage and concentrate ratio) ad libitum from day 1 to 28. This schedule will simulate the feedlot receiving period, which in commercial situations, is the period where cattle are most susceptible to opportunistic diseases and performance dictates their overall productivity through harvest (Arthington et al., 2005). Feed intake will be evaluated daily from day 1 to 28 by measuring offer and refusals. Steer shrunk bodyweight will be collected on day 1 and 29 for body weight gain calculation. Blood samples will be collected on day 0 (prior to loading), and day 1 (immediately after unloading), 4, 7, 10, 14, 21, and 28. Serum will be harvested and analyzed for concentrations of: A) cortisol (Cayman Chemical; Ann Harbor, MI) - assess neuroendocrine response, B) non-esterified fatty acids (Wako Chemicals: Dallas, TX) - evaluate body tissue degradation and nutritional status, and C) haptoglobin and ceruloplasmin (Arthington et al. 2008) - assess acute-phase protein response.

Progress 07/24/13 to 09/30/13

Outputs
Target Audience: Beef cattle producers and animal scientists Changes/Problems: We used of Meloxicam instead of sodium salicylate as our non-steroidal anti-inflammatory. We used Meloxicam because of its greater biological half-life compared to sodium salicylate, and the increasing use of Meloxicam as non-steroidal anti-inflammatory in beef cattle research We increased the number of animals from 60 to 84 steers (remain 4 steers/pen, but increased the number of pens from 15 to 21) in order to increase the statistical power of the experiment. All other procedures remained the same. Hence, the project title should change to “Use of Meloxicam to Alleviate the Transport-elicited Acute-phase Reaction in Feeder Cattle". More specifically, 84 steers weaned at 7 months of age (day -30) and vaccinated against common infectious diseases including BRD complex, were ranked by body weight and age on day 0 of the study, and assigned to 21 feedlot pens (4 steers/pen). Pens were randomly assigned to 1 of 3 treatments: 1) continuous road transport for 900 miles and daily administration of Meloxicam (1 mg/kg of BW daily) from day 0 to day 7, 2) continuous road transport for 900 miles and daily administration of lactose monohydrate (1 mg/kg of BW daily; a pharmacologically inactive excipient used in the manufacture of Meloxicam tablets) from day 0 to day 7, or 3) no transport and daily administration of lactose monohydrate (1 mg/kg of BW daily) from day 0 to day 7. The Meloxicam dose adopted herein was based on the oral administration utilized by Coetzee et al. (2010 / Journal of Animal Science 90:1026-1039) and Repenning et al. (2013 / Journal of Animal Science 91: 4965-4974) to weaned beef cattle (1 mg/kg of BW daily). Further, Meloxicam was administered to assigned steers on day 0 via oral drench (mixed with water) to modulate stress-induced inflammatory reactions elicited during transport. From day 1 to day 7 of feedlot receiving, Meloxicam was mixed in the dietary concentrate to modulate residual as well as novel stress reactions elicited by unloading and feedlot entry. What opportunities for training and professional development has the project provided? Yes, the 4 graduate students and 2 research interns were associated with the project: Thomaz Guarnieri Filho - MSc student Bruno Cappelloza - PhD student Rodrigo Marques - PhD student Maria Reis - MSc student Italo Bueno - Research intern Juliana Ranches - Research intern How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Meloxicam was orally administered (1 mg/kg of BW) to cattle prior to a 24-h transport, and during the initial 7 d of feedlot receiving. Feeding meloxicam reducedthe acute-phase protein response following feedlot entry, and improved cattle average daily gain and feed efficiency.

Publications

  • Type: Journal Articles Status: Published Year Published: 2014 Citation: 3. Guarnieri Filho, T. A., R. F. Cooke, B. I. Cappellozza, M. M. Reis, R. S. Marques, and D. W. Bohnert. 2014. Effects of meloxicam administration on physiological and performance responses of transported feeder cattle. J. Anim. Sci. doi:10.2527/jas.2014-7783.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: 3. Guarnieri Filho, T. A., R. F. Cooke, B. I. Cappellozza, M. M. Reis, R. S. Marques, and D. W. Bohnert. 2014. effects of meloxicam administration on physiological and performance responses of transported feeder cattle. Proc. West. Sec. Am. Soc. Anim. Sci. 65:35-39
  • Type: Other Status: Published Year Published: 2014 Citation: 4. Guarnieri Filho, T. A., R. F. Cooke, B. I. Cappellozza, M. M. Reis, R. S. Marques, and D. W. Bohnert. 2014. effects of meloxicam administration on physiological and performance responses of transported feeder cattle. BEEF126 In: 2014 Beef Research Report, pp 35-40