Source: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY submitted to NRP
GROWTH CHAMBER STUDY LOOKING AT HEAT TOLERANCE ADAPTATION WITHIN THE HARD MAPLE COMPLEX
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1000132
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 1, 2013
Project End Date
Jun 30, 2016
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
3 RUTGERS PLZA
NEW BRUNSWICK,NJ 08901-8559
Performing Department
Ecology, Evolution & Natural Resources
Non Technical Summary
The International Panel on Climate Change has reported that the linear warming trend in global surface temperature over the 50 years from 1956 to 2005 was nearly twice that for the 100 years from 1906 to 2005. Temperature increase is global in scale, but at high northern latitudes is noticeably more intense. Rising temperatures in the northeastern United States have impacted forest health.A breakdown in the resilience of forested landscapes opens pathways for pathogen and pest loading for vulnerable forest species, as well as a decline in abiotic stress tolerances, and long term vulnerability to climate change is anticipated. The USDA Tree Atlas models suitable habitat distributions of 134 northeastern forest species under changing climatic conditions for the next 50 years, based on three different CO2 emissions mitigation scenarios. Current forest landscapes are shifting northward and are being altered. This abrupt change in environmental conditions in the northeast is impacting forest health, ecosystem equilibrium and services, and industries dependent on the existing forest composition. In order to retain important northeastern forest species, it is necessary to investigate biological systems for sources of adaptation, providing sustainable management practices while facilitating an adaptable shift into the predicted, but not defined, future climate. This project represents a novel investigation within the hard maple complex undeveloped in the literature, which provides insights into our understanding of both climate change effects and methods for response to address such change without displacement of other components of the forested ecosystems related to the species. Our proposal is aimed at Sugar Maple, Acer saccharum Marsh., a uniquely valuable northeastern forest species that is not equipped for persistence in situ, in the face of dramatic climactic shift, and whose range is already shifting northward as a consequence. Sugar maple has been declining in the United States due to its inability to tolerate moderate periods of drought, high temperatures, and a need for soils rich in nutrients. Even while occupying only 9% of the hardwood-forested land in the United States at this time, Sugar Maple has a major impact on community identity and commerce throughout the northeastern U.S. Once supplying 80% of the world's maple sugar production, the U.S. now produces only 20% of the global product. In Vermont, the maple sugar industry provides close to 4000 seasonal jobs and generates more than $100 million annually. For an overview of the impacts of environmental change on the maple sugar industry in the U.S. see the 2001 NERA report. Biotic and abiotic disturbance events resulting from climate change such as drought, insects, and fire could have immediate effects on the presence of sugar maple in the northeast if they surpass critical thresholds. In the northeastern U.S., the pest/host interaction between Sugar Maple and the forest tent caterpillar (FTC) is an example. The rate of habitat shift exceeds the potential of species to migrate; hence, forest composition will shift. We can look within the geographic ranges of closely related species to abate the ecological, commercial, and industrial impacts resulting from loss of northeastern forest types. Woody biomass is considered an important renewable source of energy in the U.S. and around the world. The lack of synchronicity between the alteration in the forest landscape and availability of suitable habitats will have a major impact on its capacity to function as a contributor to energy solutions. Providing researchers with the limitations within forest species to thrive in future climates creates a blueprint for breeding programs with interest in this area. The naturally occurring carbon sinks that are created by the world's forests offset greenhouse gas accumulation in significant amounts and New Jersey recognizes the importance of establishing a resilient forest landscape in the future. Through annatomical, morphological and, physiological investigation, we will be able to determine the genetic variation within the hard maple group. Gauging the variation in plant response to the exisitng environment will lead us to those individuals that are best equipped for survival as global climates change and plants are exposed to novel environments.
Animal Health Component
20%
Research Effort Categories
Basic
80%
Applied
20%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20606201060100%
Goals / Objectives
1. To quantify the differences in plant physiological and morphological response among members of the hard maple complex to varying environmental factors of water availability, temperature, and soil structure in field and growth chamber studies. 2. To test to influence of cytokinin accumulation associated with plant response to increasing temperatures. 3. To evaluate Acer barbatum and Acer leucoderme provenances and identified crosses and cultivars (e.g. Acer x senecanaense) within the hard maple group as they are available, for a landscape-breeding program based on their ability to thrive at temperatures not conducive to its northern relatives.
Project Methods
A total of four field campaigns will be conducted at the extremes of the geographic regions of environmental niches. Travel will be feasilble by scheduling and using a Rutgers University Cooperative Extention van that will serve as a mobile office/lab situation. Field equipment and supplies are housed in the Urban Forestry lab at Rutgers University. Samples will be collected and morphological indicators of adaptation such as, stomatal density, trichome angle, and leaf cuticle thickness will be recorded using a dissecting scope and digital camera. The LiCOR 6400 Photsynthetic System will measure and record whole plant photosynthetic responses such as stomatal conductance, photochemical efficiency, and leaf temperature to environmental variables such as light, CO2, humidity and temperature in situ and will simultaneously establish plant response curves of light and photosynthesis versus internal levels of carbon dioxide. Hence, examination of the underlying biochemical limitations of each species in field and growth chamber studies is possible. Proposed Research sites: 1. Acer saccharum- Uihlein Sugar Maple Research& Extension Field Station Lake Placid, NY 2. Acer nigrum- The Ohio Agricultural Research Station, Wooster, Ohio. Howard Kriebel's original collection from 1957. This provides a direct link to the most thorough body of scientific work on the hard maple complex. Kriebel identified the gap in his research as lacking A. leucoderme germplasm. We will be able to round out this research since we have acquired a. leucoderme. 3. Acer barbatum- University of Florida, Gainesville, Florida 4. Acer leucoderme- Nurseries Caroliniana, Inc., North Augusta, South Carolina In a series of growth chamber studies held at the research greenhouses on Cook Campus at Rutgers University we will subject 1-2yr seedlings from our germplasm collection of the hard maple complex to increasing temperature at increments of 5 degrees every ten days. Germplasm will also be subjected to water treatments to simulate drought-like conditons to show the interaction between temperature and water. Root samples will be taken at ambient temperature and then ten days after each temperture increase and held in a deep freezer until we carry out out bio-assay for cytokinin accumulation in the roots of each species. Methodology for the cytokinin studies will follow that of the ELISA protocol. Results of the assay will be analyzed and used as direct evidence of physiological response to be used as a promising screenfor heat tolerance selection (e.g. A. x senecaense, A. saccharum x saccharum 'Green Mountain'). A secondary effort will be made to identify sources of natural and industry advertised crosses (e.g. Acer x senecaense). We will then apply the same temperature/water treatments as mentioned abouve and analyze plant response of the crosses to compare environmental tolerances to aesthetic selections that have made them marketable.

Progress 07/01/13 to 06/30/16

Outputs
Target Audience:The target audience in this period has primarily been attendees at research symposia. While the data is in process of collections and analysis, there is no wider audience outputs rationally placed beyond general presentations on tree selections and climate change. Those presentations are listed, but are not directly reporting on specific outcome of research to date. Early findings and the rationale for the project has been used in example in many extension level talks to volunteers and professions in discussions of tree selection and care in the face of climate change. Changes/Problems:A major problem developed as a family member of the Graduate student was struck mortally ill. This prolonged and intense event has slowed progress, yet the data are in place for completiion in all areas excepting the proposed cytokinin accumulation study and a reduced histological treatment. Several of the provenances have been donated to a local arboretumfor planting out in their collect to conserve the ability to further develop the histology study. What opportunities for training and professional development has the project provided?One Doctoral student is organizing and developing the data streams as the core of her dissertation. Four undergraduate students and one graduate student assisted in the completion of the varied studies. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? In a series of greenhouse studies and in observation in field container culture in Central NJ, comparisons in plant response to heat and drought have been completed. Testing populations from FL, AL,SC,TX,MN,NYand MI were compared in trails and monitored with an LiCOR 6400 for carbon assimilation, florescence and transpiration parameters. Manuscripts on physiological responses across provances in the hard maple group are in development. Members of the study were augmented with additinal plant samples an used in a genotypic analysis of 500 samples across 6 species using Genemapper 5.0 analyzing cappillary electrophoresis to link plant responses withdevelopment of neighbor-joined phylogenetic relationships in PowerMarker software. We have collected a separate set foliage smaple for the same process using the 60 year old common garden in Wooster, OH Seicrest Arboretum established by Kreibel.Students and staff from our program travelled to OH and joinded with arboretum staff in reclaiming and mapping this established grove to suppliment the recent collection with samples of known geographic and species records. A short manuscript on survivorship in this old experiment is in process, and volunteer seedling stocks have been collected to attempt documentation of species cross which are not found in literature. A manuscript is in development using the research project results to underpin a taxonomy inclusive of descriptions classification and nomenclature for a highly polymorphic grouping of species. Histologic samples have been partially developed detailing trichome and foliar tissue organization traits.

Publications

  • Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: A. Jackson. "Plasticity in population level response of Acer species complex taxa to water and temperature stress". 2nd International Conference on Urban Tree Diversity Feb 2016, Melbourne Australia


Progress 10/01/14 to 09/30/15

Outputs
Target Audience:land managers research plant biology tree care professionals landscape architecture Changes/Problems:The cytokinin work has been ambiquous, but we are getting data from photosynthetic responses. Family crisis and death of parent for the graduate student co-PI has slowed progress, but we are continuing to move forward with the project. We may consider expanding the genetic work in the coming year pending germplasm availability and funding for other sources. What opportunities for training and professional development has the project provided?Graduate student development and undergraduate student development in greenhouse study protocol and germplasm block maintenance. How have the results been disseminated to communities of interest?The study is discussed in general extension proesentation in climate change/forest and plant selection talks. No specific results have been disseminated. What do you plan to do during the next reporting period to accomplish the goals?Data analysis and manuscript development

Impacts
What was accomplished under these goals? Ecophysiology testing on a drought screen temperature screen have been completed. Genetic sequencing process is complete and in analysis. Histology tissue sampling and slide development is underway.

Publications


    Progress 10/01/13 to 09/30/14

    Outputs
    Target Audience: The target audience in this period has been attendees at research symposia. While the data is in process of collections and analysis, there is no wider audience outputs rationally placed beyond general presentations on tree selections and climate change. Those presentations are listed, but are not directly reporting on specific outcome of research to date. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Sevenundergraduate students have participated in specific tasks within thetraining and development of methods to collect the genetic, histological and eco-physiological data sets. One Doctoral student is organizing and developing the data streams as the core of her dissertation. A few other students have been trained in greenhouse management and nursery management for maintenance of the germplasm blocks. How have the results been disseminated to communities of interest? To date, 2 specific research presentations on the project have been given; one international and one national in conference scope. 8 presentations on tree selection and climate change has referenced the project and its role in understanding species aptness in a changing climate over a multi-decade urban tree service life. One international keynote address on tree selection and climate change also used this project as an example. What do you plan to do during the next reporting period to accomplish the goals? This coming year is primarily on finalizing data collection and preparation of 3-4 manuscripts. Once in place, a wider synthesis and develop of outreach materials will be addressed.

    Impacts
    What was accomplished under these goals? Germplasm collection in the field was processed for histological permanent slide mounts, as have members from multiple populations of trees within the hard maple group which have been developed for greenhouse drought testing. Slides collections have been partially developed and will employ digital imaging to develop a comparative approach with archived high resolution photomicrographs. Similarly, germplasm blocks have been sampled, for a genetic screening and analysis. Currently, half of the samples have been processed for development of phylogenetic trees in the coming year. A drought screen of sothern cohorts and a second for nothern cohorts have been conducted, however the southern cohort is being rerun to capture rebound after the watering is re-introduced. this was in part to incorporate an improved protocol, and to address observed rebound which was unexpected in the first screening.

    Publications

    • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Photosynthetic capacity of common tree species under conditions of soil metal induced stress within an urban brownfield. 99th ESA Annual Meeting, Sacramento California, August 13, 2014.


    Progress 07/01/13 to 09/30/13

    Outputs
    Target Audience: Professional horticulturalists and plant growth managers. Changes/Problems: It was determined that growth chamber use would not be suitable for our studies due to the inability of the system to control normal levels of carbon dioxide within the chambers. We have redesigned the project to be carried out in greehouse bays at the Research Greenhouses on Cook Campus at Rutgers University. What opportunities for training and professional development has the project provided? Student training and involvement: Ecophysiology using the LiCOR 6400XT system: Steven Yuen, Ross Whithead Histology: Katie O'Connell Genetics: Kathryn Kingsley Fieldwork: Ross Whitehead, Katie O'Connell Students are encouraged to seek out hypotheses based on what we see in the field and in our greenhouse data. They are also encouraged to perform literature reviews and consider publications based on their work. How have the results been disseminated to communities of interest? Through outreach talks we have communicated what we have seen thus far resulting from observations in the field and in the greenhouse.Talks have been presented in Virginia, Florida, New Jersey, Delaware, Iowa, Minnesota, and Ohio. Audiences range from tree care professionals to environmental professionals. What do you plan to do during the next reporting period to accomplish the goals? - We will begin drought studies to determine the variation in plant response based on biochemical reactions and we will conduct histology studies to compare leaf characters to determine morphological influence over plant response to drought treatments and to establish a permanent slide library of leaf anatomy. - We will continue to collect and analyze new germplasm to gain sufficient representation of the natural ranges of the maple species of interest. - We will conduct genetic analysis of the populations collected to determine the genetic relatedness of the hard maple group members. - Germination studies - Dendrochronology

    Impacts
    What was accomplished under these goals? Necessary germplasm has been collected and established for research studies: 14 populations of approximately 1200 seedlings representing the extreme areas of sugar maple natural ranges. Preliminary drought studies are complete, allowing for finialization of protocols and enabling initial drought screening studies. Ecophysiology: A/Ci curves generated for plant response to heat and drought in the field and in the greenhouse. Histology: photographic library

    Publications