PURCHASE OF AN AGILENT GAS CHROMATOGRAPHY TRIPLE QUADRUPOLE (GC/QQQ) SYSTEM, WITH ASSOCIATED AUTOSAMPLER AND SAMPLE PREPARATION EQUIPMENT

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: ACTIVE
• Funding Source: USDA COOPERATIVE AGREEMENT
• Reporting Frequency: Annual
• Accession No.: 0446902
• Project Start Date: Sep 1, 2024
• Project End Date: Aug 31, 2025
• Program Code: [(N/A)]- (N/A)

Recipient Organization
WASHINGTON STATE UNIVERSITY
2710 UNIVERSITY DRIVE
RICHLAND,WA 99352

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

10%

Research Effort Categories

Basic

90%

Applied

10%

Developmental

0%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
102	0110	1040	20%
132	0430	1060	20%
201	1131	1103	15%
102	1460	1040	15%
132	1510	1060	15%
201	1520	1103	15%

Knowledge Area
102 - Soil, Plant, Water, Nutrient Relationships; 132 - Weather and Climate; 201 - Plant Genome, Genetics, and Genetic Mechanisms;

Subject Of Investigation
1460 - Tomato; 1510 - Corn; 1131 - Wine grapes; 0110 - Soil; 0430 - Climate; 1520 - Grain sorghum;

Field Of Science
1040 - Molecular biology; 1060 - Biology (whole systems); 1103 - Other microbiology;

Keywords

smoke

mitigation

glycosides

enzymatic

hydrolysis

smoke

exposure

fuel

source

redux

thiophenols

Goals / Objectives
This Agreement will support the purchase of an Agilent GC/MS/MWS 7010D with Markes UNITY-xr with CIA Advantage-xr and ULTRA-xr Autosampler and will also support the purchase of a GERSTEL MultiPurpose Sampler Robotic Pro benchtop sample preparation system, with additional support from the Washington Wine Commission for the Gerstel sample preparation system.

Project Methods
Our collaborator at the Wine Science Center at Washington State University has access to three Agilent GC/single quadrupole MS systems and an Agilent UHPLC/QTOF-MS system for the analysis of volatile and non-volatile compounds, respectively, in grapes and wines affected by smoke exposures. In 2022, our collaborators at WSU and OSU reported the importance of a class of compounds known as thiophenols to the perception of smoke taint in smoke affected grapes and wines. While the GC/single quad MS systems have sufficient sensitivity for the analysis of volatile phenols in affected wines (ug/L concentrations), these instruments are not sufficiently sensitive for the analysis of thiophenols in affected fruit and wines, as thiophenols are present in low ng/L concentrations. The initial analytical measurements of these compounds were done with the collaboration of an industry partner using their GC/triple quadrupole MS system. The analysis of trace level compounds is best done using a GC/triple quad MS system, as these instruments have sufficient sensitivity to measure volatile compounds at concentrations less than 1.0 ng/L. Recently reported sensory thresholds for thiophenols in wines ranged from 6.0 to 25 nanograms/liter, concentrations which are within the capability of a GC/triple quad, but beyond the reach of the GC/single quad instruments currently available at the WSU Wine Science Center. To make progress in the development of analytical methods for smoke related compounds, the Collins lab seeks to purchase an Agilent 8890 GC coupled with an Agilent 7010D Triple quadrupole system.