A CHROMOSOME-SCALE GYMNOCLADUS DIOICUS GENOME AND LARGE-SCALE GERMPLASM SEQUENCING FOR TRAIT MAPPING, SEX DETERMINATION, AND CONSERVATION GENOMICS

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: ACTIVE
• Funding Source: USDA COOPERATIVE AGREEMENT
• Reporting Frequency: Annual
• Accession No.: 0445501
• Project Start Date: Mar 1, 2024
• Project End Date: Feb 28, 2025
• Program Code: [(N/A)]- (N/A)

Recipient Organization
Hudson-Alpha Institute for Biotechnology
601 Genome Way
Huntsville,AL 35806

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

80%

Research Effort Categories

Basic

10%

Applied

80%

Developmental

10%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
202	2110	1080	100%

Knowledge Area
202 - Plant Genetic Resources;

Subject Of Investigation
2110 - Ornamental trees and shrubs;

Field Of Science
1080 - Genetics;

Keywords

kentucky

coffeetree

gymnocladus

dioicus

genetic

diversity

sexual

system

Goals / Objectives
1. Identify the sexual system and genome size of Gymnocladus dioicus. 2. Characterize germplasm diversity.

Project Methods
Deoxyribonucleic acid (DNA) isolation will be executed using standard cetyltrimethylammonium bromide (CTAB) extraction. Illumina polymerase chain reaction (PCR) genome sequencing will be used to identify the sexual system of G. dioicus.