STACKING RNAI-BASED RESISTANCE TO PHYTOPHTHORA, CROWN GALL, AND NEMATODES IN ALMOND AND WALNUT ROOTSTOCKS

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: ACTIVE
• Funding Source: USDA COOPERATIVE AGREEMENT
• Reporting Frequency: Annual
• Accession No.: 0443615
• Project Start Date: Nov 1, 2022
• Project End Date: Feb 28, 2025
• Program Code: [(N/A)]- (N/A)

Recipient Organization
UNIVERSITY OF CALIFORNIA, DAVIS
410 MRAK HALL
DAVIS,CA 95616-8671

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

30%

Research Effort Categories

Basic

70%

Applied

30%

Developmental

0%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
212	1212	1160	50%
212	1213	1160	50%

Knowledge Area
212 - Pathogens and Nematodes Affecting Plants;

Subject Of Investigation
1212 - Almond; 1213 - Walnut;

Field Of Science
1160 - Pathology;

Keywords

rnai

phytophthora

rootstock

crown

gall

nematodes

Goals / Objectives
Develop walnut and almond RNAi lines for resistance to the three main soilborne diseases (Phytophthora root and crown rot, crown gall, and root-lesion nematodes)

Project Methods
Cooperator will construct transformation vectors for RNAi against Phytophthora, then co-transform somatic embryos with two vectors, which confer resistance against Phytophthora and crown gall (the vector already available). Cooperator will subsequently propagate transgenic embryo lines, inoculate them with pathogens and evaluate resistance. Transgenic lines showing resistance will then be propagated, rooted, and grown in pots. Cooperator will conduct greenhouse trials for Phytophthora and crown gall resistance.