Source: UNIVERSITY OF GEORGIA submitted to NRP
ROLE OF BACTERIAL EXOPOLYSACCHARIDE COMPOSITION AND STRUCTURE IN BIOFILM FORMATION, VISCOSITY AND SUSCEPTIBILITY TO DEXTRANASE PROCESSING AID
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
ACTIVE
Funding Source
USDA COOPERATIVE AGREEMENT
Reporting Frequency
Annual
Accession No.
0443398
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Aug 1, 2023
Project End Date
Aug 31, 2027
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF GEORGIA
200 D.W. BROOKS DR
ATHENS,GA 30602-5016
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
40%
Research Effort Categories
Basic
20%
Applied
40%
Developmental
40%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
5012020200050%
5032030202030%
5112010200020%
Knowledge Area
511 - New and Improved Non-Food Products and Processes; 501 - New and Improved Food Processing Technologies; 503 - Quality Maintenance in Storing and Marketing Food Products;

Subject Of Investigation
2010 - Sugar beet; 2020 - Sugar cane; 2030 - Sweet sorghum;

Field Of Science
2020 - Engineering; 2000 - Chemistry;
Goals / Objectives
Characterize exopolysaccharides (EPS) produced by bacterial isolate cultures and biofilms. Determine size, glycosyl composition, linkage, and branching of EPS and investigate susceptibility of EPS structures to dextranase enzyme.
Project Methods
This work will be conducted over four years. EPS and biofilm samples produced by bacterial strains of interest will be sent to the Cooperator for structural analysis. The Cooperator specializes in methods to analyze and perform any extra purification that might be required on biofilms in order to perform structural analysis. The progress of the purification and the chemical nature of the analyzed sample will be obtained by glycosyl composition analysis of monosaccharides (e.g., per-O-trimethylsilyl (TMS)-methyl glysosides, aldtiol acetates, heptafluorobutyric anhydride- methyl glycosides) utilizing gas chromatography-mass spectroscopy (GC-MS)/gas chromatography-flame ionization detector (GC-FID)/gas chromatography-chemical ionization (GC-CI) or high-performance anion exchange chromatography/pulsed amperometric detection (HPAEC) analysis of mono- and oligosaccharides. The structural properties of the polysaccharide are revealed by combining monosaccharide composition with glycosyl linkage of neutral and acidic sugars by the method of partially methylated alditol acetates (PMAA). Glycosyl sequence of the complex carbohydrates will be determined by performing 1D and 2D homo- and heteronuclear Nuclear Magnetic Resonanace (NMR). A high-resolution matrix assisted laser desorption ionization-time of flight-mass spectroscopy (MALDI-TOF) MS and MS/MS electrospray ionization (ESI-MS) mass spectrometry further supports the structural properties of the polysaccharides. Molecular weight and complexity of large polymers can also be determined by size exclusion chromatography (SEC)-multi-angle light scattering (MALS).