DEVELOP MOLECULAR DIAGNOSTIC TOOLS TO DETERMINE STRAIN AND MATING STATUS OF FRUIT FLY INCURSIONS

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: ACTIVE
• Funding Source: USDA COOPERATIVE AGREEMENT
• Reporting Frequency: Annual
• Accession No.: 0442648
• Project Start Date: Aug 1, 2022
• Project End Date: Dec 31, 2023
• Program Code: [(N/A)]- (N/A)

Recipient Organization
UNIV OF HAWAII
3190 MAILE WAY
HONOLULU,HI 96822

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

100%

Research Effort Categories

Basic

0%

Applied

100%

Developmental

0%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
211	0999	1130	50%
215	1099	1130	50%

Knowledge Area
211 - Insects, Mites, and Other Arthropods Affecting Plants; 215 - Biological Control of Pests Affecting Plants;

Subject Of Investigation
1099 - Tropical/subtropical fruit, general/other; 0999 - Citrus, general/other;

Field Of Science
1130 - Entomology and acarology;

Keywords

tephritidae

mexfly

medfly

mexican

fruit

fly

meditteranean

fruit

fly

anastrepha

ceratitis

Goals / Objectives
Develop molecular diagnostic tools to determine strain and mating status of fruit fly incursions.

Project Methods
1. Select candidate autosomal strain-specific and sex-linked markers. 2. Develop diagnostic assay discriminating males from females and GSS and wild. 3. Perform experimental crosses to validate diagnostic assay. 4. Develop methods to quantify mating of wild females to GSS males vs wild males.