Source: MICHIGAN STATE UNIV submitted to NRP
NSI: DEVELOPING SOYBEAN VARIETIES WITH RESISTANCE TO SCLEROTINIA STEM ROT
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
ACTIVE
Funding Source
USDA COOPERATIVE AGREEMENT
Reporting Frequency
Annual
Accession No.
0442184
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 1, 2022
Project End Date
Dec 31, 2025
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
MICHIGAN STATE UNIV
(N/A)
EAST LANSING,MI 48824
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
35%
Research Effort Categories
Basic
35%
Applied
35%
Developmental
30%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
21218201080100%
Knowledge Area
212 - Pathogens and Nematodes Affecting Plants;

Subject Of Investigation
1820 - Soybean;

Field Of Science
1080 - Genetics;
Goals / Objectives
1. To continue the breeding pipeline for enhancing resistance to soybean white mold; 2. To identify the resistance quantitative trait loci and develop markers using nested association mapping populations; and 3. To continue the evaluation of advanced breeding lines for enhanced resistance and release the best lines as varieties.
Project Methods
For Objective 1, we will make at least 10 new cross combinations focusing on yield, protein, and the white mold disease package. The new cross combinations will be made between elite lines carrying special traits such as high yield, high protein, and high oleic, and white mold resistance lines, which were identified from previous research. In addition, more than 100 lines from prior year Advanced Yield Trials will be evaluated. For Objective 2, a previously developed nested association mapping (NAM) population will be used to generate a high-density genetic map. Leaf samples of F4 individuals for each line will be bulk harvested and stored in a freezer and DNA samples will be isolated from the leaf samples using standard CTAB method. DNA for each line will be assessed with the SoySNP3K bead chips to genotype the NAM population. High-resolution genetic maps and hapmaps will be generated using JoinMap 4.0 and Tassel software. For Objective 3, over 100 advanced breeding lines (F7 & F8) derived from multiple sources of resistance to white mold will be evaluated for resistance to white mold. An irrigation system will be used to provide the wet condition to promote the disease development during the flowering period. Five to 10 lines will be selected and subsequently tested in the Uniform Soybean Tests â¿¿ Northern Region (19 locations in 10 northern US states and 1 Canadian province) for yield and other agronomic traits. The best lines will be released to the public or used as new parental lines in future evaluations.