Source: WASHINGTON STATE UNIVERSITY submitted to NRP
GENETIC DIVERSITY OF IMPATIENS NECROTIC SPOT VIRUS (INSV) ISOLATES AFFECTING IMPORTANT VEGETABLE AND HORTICULTURAL CROPS
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
ACTIVE
Funding Source
USDA COOPERATIVE AGREEMENT
Reporting Frequency
Annual
Accession No.
0442120
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
May 1, 2022
Project End Date
Dec 30, 2023
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
WASHINGTON STATE UNIVERSITY
240 FRENCH ADMINISTRATION BLDG
PULLMAN,WA 99164-0001
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2111122104010%
2121429110110%
2111430113070%
2121460116010%
Knowledge Area
212 - Pathogens and Nematodes Affecting Plants; 211 - Insects, Mites, and Other Arthropods Affecting Plants;

Subject Of Investigation
1460 - Tomato; 1430 - Greens and leafy vegetables; 1122 - Strawberry; 1429 - Cucurbits, other;

Field Of Science
1040 - Molecular biology; 1101 - Virology; 1130 - Entomology and acarology; 1160 - Pathology;
Goals / Objectives
Perform genome sequencing of current and historical Impatiens necrotic spot virus (INSV) isolates from major lettuce growing regions in California and Arizona, as well as isolates from horticultural crops, including dahlia. Understanding the genetic diversity of INSV isolates will provide greater knowledge on the biology of the virus and its potential emergence as an important pathogen of lettuce in California and Arizona.
Project Methods
INSV isolates have been obtained from lettuce in 2019 to 2022 in the Salinas Valley (Monterey County) and neighboring counties on the Central Coast of California (San Benito and Santa Cruz County). Isolates from the desert region in southern California (Imperial County and Coachella Valley) and Arizona (Yuma County) were also obtained from lettuce in 2021 and 2022. A second sample of INSV from 2008 was also obtained from a freezer stock at USDA in Salinas. All samples will be extracted for RNA at USDA Salinas and sent to the Cooperator (Washington State University) and sequenced to obtain genetic sequences for all the INSV isolates. Additionally, RNA will be isolated from INSV-infected dahlia and other horticultural crops by the Cooperator or USDA Salinas. All RNAs will be sequenced, processed, and analyzed by the Cooperator. At least 20 isolates will be sequenced and analyzed.