Progress 10/01/23 to 09/30/24
Outputs
PROGRESS REPORT Objectives (from AD-416): Objective 1: Ascertain the viral ecology and factors mediating the introduction and expansion of VSV in the U.S. Objective 1A. Identify viral genetic determinants mediating emergence of epidemic VSV in the U.S. as well as adaptation to insect and animal hosts. Objective 1B. Characterize epidemiological, biotic and abiotic factors associated with vectorial capacity, emergence, incursion, and expansion of VSV from endemic areas into the U.S. Objective 2. Develop intervention strategies to minimize the impact of VSV disease outbreaks. Objective 2A. Retrospectively integrate and harmonize environmental, vector, host, and viral variables with disease occurrence data to support the development of predictive multi-scale big data models for VSV. Objective 2B. Develop model-based early warning systems to predict future incursions of VSV from Mexico to the U.S. Objective 2C. Identify vector transmission control strategies based on our understanding of virus-vector-host interactions. Approach (from AD-416): Objective 1A:The effect of genetic changes on virulence and transmissibility will be investigated with three approaches: 1) investigating virulence in pigs and vector transmissibility in relevant vectors (e.g., midges and black flies); 2) characterizing genomes of the 2019-2020 VSIV U.S. epidemic strains using comparative genomics to identify genetic differences between epidemic and endemic VSIV in Mexico. 3) if different endemic vs. epidemic lineages are identified, then characterize the virulence of these strains. Objective 1B. We will quantify the occurrence of VS cases across space and time in the endemic region in Mexico. Together these data will enable us to quantify the occurrence of VS cases across space and time in the endemic region. If successful, we expect to identify associations between VS cases and environmental/ecological factors. We will employ the big data model integration (BDMI) approach used previously by our VSV-GC collaborators. Objective 2A: Temporal relationships between VS occurrences and variables representing environmental factors relevant to VS transmission will be evaluated. First, municipality level VS occurrence data from 1981 ⿿ 2020 provided by SENASICA will be temporally binned based on incursion years into the U.S.: 1985, 1995, 2004, 2012, or 2019. Next, collated datasets representing environmental, ecological and/or biological factors relevant VS occurrences across Mexico for each year identified from Goal 1B.1 will be synchronized with the SENASICA dataset. Initially, time-series analysis of VS occurrence within temporal bins across geographic space will be performed. This analysis will help determine whether VS occurrence and spread outside of the endemic region occurs randomly or non-randomly in space and time prior to incursion in the U.S. We will then test whether identified factors reliably predict incursions to the U. S. using multivariate analyses and, if appropriate, machine learning as previously described. Analyses may reveal genomic markers of virulence to inform surveillance in Mexico and early warning programs. If successful, we expect to identify a reliable set of environmental/ecological/ biological variables that can be used to inform early warning metrics in predictive modeling of VSV incursion. Objective 2B: We hypothesize that VSV infection will alter photosensory perception, altering the effectiveness of current phototaxis-dependent surveillance traps and management strategies. If successful, we will determine the effects of VSV infection on midge photoattraction behavior and on the expression of vision or other neurosensory genes. In 2024, substantial progress was made addressing all the unit objectives for this research project. In addition to the scientific progress, the Vesicular Stomatitis Virus (VSV) work transferred from Orient Point, New York, to Manhattan, Kansas. This required the setting up of new lab spaces, Institutional Biosafety Committee (IBC) and Institutional Animal Care and Use Committee (IACUC) protocols, and VSV permits to establish the VSV work in Manhattan, Kansas. We have continued to collaborate within the Grand Challenge partners (Animal and Plant Health Inspection Service (APHIS), SciNet, and continued partnerships with New Mexico State University, Colorado State University, Kansas State University, and the Animal Health Service in Mexico). Objective 1A, identify viral genetic determinants mediating emergence of epidemic VSV in the U.S. as well as adaptation to insect and animal hosts. An important accomplishment was the successful in-vivo characterization of the infectious-clone derived Vesicular stomatitis New Jersey virus (VSNJV) epidemic lineage 1.1 containing 2 key VSV Matrix protein amino acid mutations. This mutant virus was compared to its wild-type progenitor for its virulence in our previously established pig inoculation model. The results showed clear differences in virulence between these viruses that might suggest mechanisms of virulence and emergence of the epidemic viral lineage. Further characterization of the study samples is in progress to understand the factors involved in mediating the virulence. In addition, we initiated an immunology-pathogenesis study aimed at determining the host cells required for limiting the replication of VSV in mammalian hosts. Further characterization of Natural Killer cells transcriptome in response to VSV infection is in progress. In May 2023, APHIS⿿s National Veterinary Services Laboratories (NVSL), confirmed a case of VSNJV on an equine premises in San Diego County, California. The virus continued to spread through California through early January 2024. Based on the initial outbreak in San Diego County and transition to the San Joaquin Valley, collections for black flies, biting midges, and sand flies will be scheduled from San Diego County up to Placer County in the Sierra Nevada. Collaborations with Placer County Mosquito and Vector Control have been established for understanding the nature of the insects at the northern most point of the California outbreak to the initial outbreak area and already established collaborations in San Diego County. Objective 1B, through an agreement with New Mexico State University (NMSU) we continued research activities in Chiapas, Mexico in collaboration with Mexico⿿s National Animal Health Service; Servico Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA). A study to elucidate the spatiotemporal dynamics of different VSV vectors (black flies, sand flies, midges, and mosquitoes) on five farms in the State of Chiapas was initiated. Additionally, a long-term dataset on all reported VSV cases over the last thirty years continues to be analyzed, along with remotely sensed data on climate, land cover/land use, and distribution of domestic animals and has identified key variables regarding the distribution of VSV within its endemic range. Finally, we have expanded our collaboration in Mexico with SENASICA to include two additional study sites, one in Jalisco and one in Chihuahua. The Chihuahua location was the first of the two sites to be focused on in 2024 and field sites and laboratory resources have been identified and will be ready in 2025 to provide collections and data. The establishment of the Jalisco site is currently under development. With these two additional sites, we will be able to document the expansion of epidemic strains of VSV within Mexico to inform the development of predictive models for the occurrence of VSV epidemics in the U.S. In particular, we will be able to see when and through what vector(s) a VSV lineage moves from Chiapas up to the Mexico-U.S. border. Objective 2A. In 2024 we continued the detailed analysis of the 2014-2015 VSNJV outbreak and 2019-2020 Vesicular Stomatitis Virus Indiana (VSIV) carried out through collaborations within the ARS Grand Challenge synergy project. It was determined that for the 2019-2020 VSIV outbreak there were distinct viral lineages during the incursion year in Colorado and New Mexico compared to the expansion years in multiple States (Kansas, Nebraska, Missouri, Wyoming). The genetic data as well as environmental variables are being used to refine the predictive models previously developed for VSNJV. ACCOMPLISHMENTS 01 Development of insecticide that can control midge larvae. Biting midges are one of the vectors identified in the U.S. as being competent to transmit Vesicular Stomatitis Virus to livestock. Outbreaks of Vesicular Stomatitis Virus result in quarantines of farms and facilities with signs of the disease as it causes similar lesions compared to Foot and Mouth Disease Virus which is a major agricultural disease of concern and requires a diagnostic to differentiate the infections during the quarantine. Controlling biting midge populations is one way to mitigate emergence of Vesicular Stomatitis Virus outbreaks. ARS scientists in Manhattan, Kansas, in collaboration with Kansas State University developed silver nanoparticles which can be efficient insecticides for a number of arthropod larvae including mosquitoes. For the first time, biting midge larvae were observed to be susceptible to silver nanoparticles with up to 75% mortality within 24 hours against this important agricultural pest which could play a role in an environmentally safer formulation compared to traditional insecticides. 02 Identification of key circulating black flies in New Mexico during 2020 Vesicular Stomatitis Virus Outbreak. ARS scientist in Manhattan, Kansas, in collaboration with scientist from New Mexico State University identified key circulating black flies in New Mexico during 2020 Vesicular Stomatitis Virus Outbreak. Black flies are an agricultural pest of concern as they can have high abundance coinciding with aggressive feeding behavior during the spring and summer in some regions of North America. Black flies are one of the vectors identified in the United States as being competent to transmit Vesicular Stomatitis Virus to livestock. Clinical vesicular stomatitis can be observed in hoofed domestic livestock (e.g. horses, cattle, pigs) which can result in vesicular lesions in mouth and feet or udders, resulting in lameness, reduced milk production, and weight loss. In cattle and pigs, the disease resembles foot-and-mouth disease, a high consequence transboundary disease not currently in the United States. Outbreaks of Vesicular Stomatitis Virus lead to substantial economic losses, caused by animal quarantines and decreased productivity. New Mexico has been a part of multiple Vesicular Stomatitis Virus incursions from Mexico into the United States and these outbreaks have been associated with rivers that are black fly habitats. Sites on the Rio Grande River were setup for collections of black flies in New Mexico in 2020 during a Vesicular Stomatitis Virus outbreak. Analysis of the black fly catch revealed 2 black fly species positive for the virus that had not been previously associated with Vesicular Stomatitis Virus. These 2 species were only found in 2020 catches compared to 2021-2022 implicating them in the 2020 outbreak. Understanding the virus-vector dynamics at incursion points into the United States can lead to early warning systems and control mechanisms to mitigate the effects of Vesicular Stomatitis Virus incursions.
Impacts
(N/A)
Publications
- Carpenter, M., Kopanke, J., Lee, J., Rodgers, C., Reed, K., Sherman, T., Graham, B., Cohnstaedt, L.W., Wilson, W.C., Stenglein, M., Mayo, C. 2024. Evaluating temperature effects on bluetongue virus serotype 10 and 17 coinfection in Culicoides sonorensis. International Journal of Molecular Sciences. 25(5). Article 3063. https://doi.org/10.3390/ijms25053063.
- Whelpley, M.J., Zhou, L.H., Rascon, J., Payne, B., Moehn, B., Young, K.I., Mire, C.E., Peters, D.P., Rodriguez, L.L., Hanley, K.A. 2024. Community composition of black flies during and after the 2020 vesicular stomatitis virus outbreak in southern New Mexico, USA. Parasites & Vectors. 17. Article 93. https://doi.org/10.1186/s13071-024-06127-6.
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Progress 10/01/22 to 09/30/23
Outputs
PROGRESS REPORT Objectives (from AD-416): Objective 1: Ascertain the viral ecology and factors mediating the introduction and expansion of VSV in the U.S. Objective 1A. Identify viral genetic determinants mediating emergence of epidemic VSV in the U.S. as well as adaptation to insect and animal hosts. Objective 1B. Characterize epidemiological, biotic and abiotic factors associated with vectorial capacity, emergence, incursion, and expansion of VSV from endemic areas into the U.S. Objective 2. Develop intervention strategies to minimize the impact of VSV disease outbreaks. Objective 2A. Retrospectively integrate and harmonize environmental, vector, host, and viral variables with disease occurrence data to support the development of predictive multi-scale big data models for VSV. Objective 2B. Develop model-based early warning systems to predict future incursions of VSV from Mexico to the U.S. Objective 2C. Identify vector transmission control strategies based on our understanding of virus-vector-host interactions. Approach (from AD-416): Objective 1A:The effect of genetic changes on virulence and transmissibility will be investigated with three approaches: 1) investigating virulence in pigs and vector transmissibility in relevant vectors (e.g., midges and black flies); 2) characterizing genomes of the 2019-2020 VSIV U.S. epidemic strains using comparative genomics to identify genetic differences between epidemic and endemic VSIV in Mexico. 3) if different endemic vs. epidemic lineages are identified, then characterize the virulence of these strains. Objective 1B. We will quantify the occurrence of VS cases across space and time in the endemic region in Mexico. Together these data will enable us to quantify the occurrence of VS cases across space and time in the endemic region. If successful, we expect to identify associations between VS cases and environmental/ecological factors. We will employ the big data model integration (BDMI) approach used previously by our VSV-GC collaborators. Objective 2A: Temporal relationships between VS occurrences and variables representing environmental factors relevant to VS transmission will be evaluated. First, municipality level VS occurrence data from 1981 ⿿ 2020 provided by SENASICA will be temporally binned based on incursion years into the U.S.: 1985, 1995, 2004, 2012, or 2019. Next, collated datasets representing environmental, ecological and/or biological factors relevant VS occurrences across Mexico for each year identified from Goal 1B.1 will be synchronized with the SENASICA dataset. Initially, time-series analysis of VS occurrence within temporal bins across geographic space will be performed. This analysis will help determine whether VS occurrence and spread outside of the endemic region occurs randomly or non-randomly in space and time prior to incursion in the U.S. We will then test whether identified factors reliably predict incursions to the U. S. using multivariate analyses and, if appropriate, machine learning as previously described. Analyses may reveal genomic markers of virulence to inform surveillance in Mexico and early warning programs. If successful, we expect to identify a reliable set of environmental/ecological/ biological variables that can be used to inform early warning metrics in predictive modeling of VSV incursion. Objective 2B: We hypothesize that VSV infection will alter photosensory perception, altering the effectiveness of current phototaxis-dependent surveillance traps and management strategies. If successful, we will determine the effects of VSV infection on midge photoattraction behavior and on the expression of vision or other neurosensory genes. Substantial overall progress was made in this research project addressing all the objectives during FY 2023. An important event occurred in 2023: the incursion of Vesicular Stomatitis New Jersey virus (VSNJV) in the United States, specifically in California. This represented an opportunity to collaborate with Animal and Plant Health Inspection Service ⿿ National Veterinary Services Laboratories (APHIS-NVSL) in the genomic characterization and determine the phylogeographic origin of the new Vesicluar Stomatitis Virus (VSV) incursion. The results of these studies are ongoing and will be reported in FY 2024. We have continued to collaborate within the Grand Challenge partners (APHIS), SciNet, and continued partnerships with New Mexico State University, Colorado State University, Kansas State University, and the Animal Health Service in Mexico (SENASICA). Objective 1A. An important accomplishment was the successful in-vivo characterization of the infectious-clone derived VSNJV epidemic lineage 1. 1 containing the 5 most significant amino acid mutations found in endemic lineage 1.2. This mutant virus was compared to its wild-type progenitor for its virulence in our previously established pig inoculation model. The results showed clear differences in virus shedding between these viruses that might suggest mechanisms of emergence and spread of the epidemic viral lineage. Further studies are in progress. In addition, through a collaboration with Kansas State University, we initiated a molecular pathogenesis study aimed at determining the cellular host proteins required for efficient growth of VSV in mammalian cells. There were 5 candidate host factors identified that seem to influence the virus to reproduce in cells. The identification of host factors involved in the VSV replication cycle will increase our knowledge of the molecular pathogenesis of the virus and influence the development of potential antivirals. During the 2019-2020 Vesicular Stomatitis Indiana Virus (VSIV) outbreaks there was great progress made under a collaborative agreement with Colorado State University, we finalized the genomic analysis of over 100 near ⿿ full length genomes of VS Indiana (VSIV) viruses from Colorado, Texas, New Mexico, Kansas, Missouri, Nebraska, and Wyoming, and showed the genetic diversity associated with each of the years and geographical areas of the 2019-2020 outbreaks. This represents the most extensive and comprehensive analysis of VSIV to date and demonstrated that a single incursion of a viral lineage from Mexico was the origin of the outbreak. In May 2023, APHIS⿿s National Veterinary Services Laboratories (NVSL), confirmed a case of VSNJV on an equine premises in San Diego County, California. This is the first report of VSV in California in over three decades. In collaboration with NVSL colleagues, ARS scientists in Orient Point, New York, and Manhattan, Kansas, determined the origin of the outbreak causing strain and tracked it to a viral genetic lineage (lineage 2.2) of Central American origin that was detected circulating in Mexico as early as 2008, as documented in a manuscript published in 2012. We identified this lineage as of concern as it represented a new introduction of this virus into Mexico with the potential of expanding into the U.S. Furthermore, this epidemic lineage affected the San Diego Safari Park where 21 specimens from two species of rhinoceros (Asian and African) were clinically affected, some of them severely. ARS scientists are collaborating with APHIS, the safari park scientists and clinical veterinarians to control the disease in affected animals. Additional studies are being carried out at the safari park including serological sampling of other animal species to determine the extent of animal species infected and sampling of insects to determine the potential vectors spreading the virus in and around the safari park. Objective 1B. Through an agreement with New Mexico State University (NMSU) we continued research activities in Chiapas, Mexico in collaboration with Mexico⿿s National Animal Health Service SENASICA. A study to elucidate the spatiotemporal dynamics of different VSV vectors (black flies, sand flies, midges, and mosquitoes) on five farms in the State of Chiapas, has made great progress, with thousands of insects captured at the five sites. Further, testing of the insects by real-time Polymeras Chain Reaction (rt-PCR) at the Mexico laboratory yielded a number of virus-positive insect pools for both VS New Jersey and Indiana serotypes of VSV. In addition, clinical cases of VSV were reported and characterized by the central laboratory in Mexico yielding positive results for virus detection. These samples were shipped to the ARS laboratory at Plum Island, New York, for further characterization and genomic sequencing. In addition, a long-term dataset on all reported VSV cases over the last thirty years is being analyzed, along with remotely sensed data on climate, land cover/land use, and distribution of domestic animals and has identified key variables regarding the distribution of VSV within its endemic range. This study is nearing completion. Finally, a current research collaboration with Mexico will be expanded to include two additional study sites in Central and Northern Mexico. The aim of this new project is to document the expansion of epidemic strains of VSV within Mexico to inform the development of predictive models for the occurrence of VSV epidemics in the U.S. Objective 2A. During the past year a detailed analysis of the 2014-2015 VSNJV outbreak and 2019-2020 VSIV is being carried out through collaborations within the ARS Grand Challenge synergy project. It was determined that for the 2019-202 VSIV outbreak there were distinct viral lineages during the incursion year in Colorado and New Mexico compared to the expansion years in multiple States (Kansas, Nebraska, Missouri, Wyoming). The genetic data as well as environmental variables are being used to refine the predictive models previously developed for VSNJV. An abstract entitled ⿿The relationship between broad-scale climate variability and the expansion of a vector-borne disease in North America describing this effort was presented at the Spatial Statistics 2023 meeting. ACCOMPLISHMENTS 01 The role of innate immune response in Vesicular Stomatitis Virus (VSV) pathogenesis: Applications to animal and public health. Innate immunity is key to the prevention and control of viral disease. Vesicular stomatitis has been previously used to study innate responses, particularly interferon induction and response pathways. In addition to being a disease-causing virus, VSV is being utilized as an effective vaccine viral vector (e.g., Ebola virus vaccine) and as a cancer treatment as an oncolytic agent. ARS scientists in Orient, New York, and Manhattan, Kansas, previously reported that a single amino-acid substitution in one protein (M51R) of Vesicular Stomatitis New Jersey Virus (VSNJV) has a fully attenuating effect in a natural host (pigs) with attenuation being mediated by the inability of this mutant to shut down host innate immune response. Here ARS researchers further characterized the gene expression of cells infected with M51R compared to the wild type progenitor virus. The results showed that the cells infected with mutant virus expressed significantly higher levels of multiple interferon and other innate response genes within the innate response and cell-death pathways. The identification of the cellular genes regulated by VSV will inform the development of better antiviral treatments, improved vaccines, and safer and effective targeted cancer treatment options. This research is a good example of cross-cutting science applied to multiple fields in animal and public health.
Impacts
(N/A)
Publications
- Bertram, M.R., Rodgers, C., Reed, K., Velazquez Salinas, L., Pelzel- Mccluskey, A., Mayo, C., Rodriguez, L.L. 2023. Vesicular stomatitis Indiana virus near-full-length genome sequences reveal low genetic diversity during the 2019 outbreak in Colorado, USA. Frontiers in Veterinary Science. 10. Article 1110483. https://doi.org/10.3389/fvets. 2023.1110483.
- Leon, B., Sanchez, C., Rodriguez, L.L., Cordero-Solorzano, J.M. 2022. Vesicular stomatitis virus isolated from a bovine brain sample in Costa Rica. Microbiology Resource Announcements. https://doi.org/10.1128/mra. 00737-22.
- Velazquez Salinas, L., Medina, G.N., Valdez, F., Collinson, S., Zarate, S., Zhu, J.J., Rodriguez, L.L. 2023. Exploring the molecular basis of vesicular stomatitis virus pathogenesis in swine: insights from expression profiling of primary macrophages infected with M51R mutant virus. Pathogens. 12(7). Article 896. https://doi.org/10.3390/pathogens12070896.
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Progress 10/01/21 to 09/30/22
Outputs
PROGRESS REPORT Objectives (from AD-416): Objective 1: Ascertain the viral ecology and factors mediating the introduction and expansion of VSV in the U.S. Objective 1A. Identify viral genetic determinants mediating emergence of epidemic VSV in the U.S. as well as adaptation to insect and animal hosts. Objective 1B. Characterize epidemiological, biotic and abiotic factors associated with vectorial capacity, emergence, incursion, and expansion of VSV from endemic areas into the U.S. Objective 2. Develop intervention strategies to minimize the impact of VSV disease outbreaks. Objective 2A. Develop model-based early warning systems to predict future incursions of VSV from Mexico to the U.S. Objective 2B. Identify vector transmission control strategies based on our understanding of virus-vector-host interactions. Approach (from AD-416): Objective 1A:The effect of genetic changes on virulence and transmissibility will be investigated with three approaches: 1) investigating virulence in pigs and vector transmissibility in relevant vectors (e.g., midges and black flies); 2) characterizing genomes of the 2019-2020 VSIV U.S. epidemic strains using comparative genomics to identify genetic differences between epidemic and endemic VSIV in Mexico. 3) if different endemic vs. epidemic lineages are identified, then characterize the virulence of these strains. Objective 1B. We will quantify the occurrence of VS cases across space and time in the endemic region in Mexico. Together these data will enable us to quantify the occurrence of VS cases across space and time in the endemic region. If successful, we expect to identify associations between VS cases and environmental/ecological factors. We will employ the big data model integration (BDMI) approach used previously by our VSV-GC collaborators. Objective 2A: Temporal relationships between VS occurrences and variables representing environmental factors relevant to VS transmission will be evaluated. First, municipality level VS occurrence data from 1981 � 2020 provided by SENASICA will be temporally binned based on incursion years into the U.S.: 1985, 1995, 2004, 2012, or 2019. Next, collated datasets representing environmental, ecological and/or biological factors relevant VS occurrences across Mexico for each year identified from Goal 1B.1 will be synchronized with the SENASICA dataset. Initially, time-series analysis of VS occurrence within temporal bins across geographic space will be performed. This analysis will help determine whether VS occurrence and spread outside of the endemic region occurs randomly or non-randomly in space and time prior to incursion in the U.S. We will then test whether identified factors reliably predict incursions to the U. S. using multivariate analyses and, if appropriate, machine learning as previously described. Analyses may reveal genomic markers of virulence to inform surveillance in Mexico and early warning programs. If successful, we expect to identify a reliable set of environmental/ecological/ biological variables that can be used to inform early warning metrics in predictive modeling of VSV incursion. Objective 2B: We hypothesize that VSV infection will alter photosensory perception, altering the effectiveness of current phototaxis-dependent surveillance traps and management strategies. If successful, we will determine the effects of VSV infection on midge photoattraction behavior and on the expression of vision or other neurosensory genes. Despite significant disruption due to COVID-19 restrictions, the Vesicular Stomatitis Virus (VSV) teams in New York and Kansas were able to make substantial progress on all milestones of the newly approved project. Under Objective 1, under a collaborative agreement established in 2021 with Colorado State University, over 400 strains of VSIV from the 2019 outbreak were fully sequenced. The sequences are in the process of being analyzed for phylogeographic analysis. Another important accomplishment was the successful derivation of an infectious Vesicular Stomatitis New Jersey Virus (VSNJV) lineage 1.1 containing the 5 most significant amino acid mutations found in endemic lineage 1.2. This virus is in the process of being characterized both in vitro and in-vivo, we will report the findings in FY23. Though a NACA with New Mexico State University, we were able to travel to Chiapas, Mexico to meet with our collaborators in SENASICA (the Mexican counterpart to USDA). We planned and launched a study to elucidate the spatiotemporal dynamics of different VSV vectors (black flies, sand flies, midges, and mosquitoes) on five farms in the State of Chiapas, an endemic that regularly (almost annually) report cases of VSV. We coupled this with regular reporting of any VSV cases on each farm, and with collection of sera on each farm from cattle over a broad range of ages. Insect vectors are being screened by real-time Rt-PCT for VSV at the SENASICA lab facility in Chiapas. Positive pools of insects and sera from over 150 farm animals were shipped to the USDA-Plum Island Animal Disease Center for virus and antibody assays. In addition, we have acquired from our collaborators at SENASICA a long-term dataset on all reported VSV cases over the last thirty years and, using this data, along with remotely sensed data on climate, land cover/land use, and distribution of domestic animals will determine key variables that predict the distribution of VSV within its endemic range. This study is nearing completion and manuscript preparation is in progress.
Impacts
(N/A)
Publications
- Rozo-Lopez, P., Pauszek, S.J., Velazquez Salinas, L., Rodriguez, L.L., Park, Y., Drolet, B.S. 2022. Comparison of endemic and epidemic vesicular stomatitis virus lineages in Culicoides sonorensis midges. Viruses. 14(6) :1221-1233. https://doi.org/10.3390/v14061221.
- Velazquez-Salinas, L., Pauszek, S.J., Holinka, L.G., Gladue, D.P., Rekant, S.I., Bishop, E.A., Stenfeldt, C., Verdugo-Rodriguez, A., Borca, M.V., Rodriguez, L.L., Arzt, J. 2020. A single amino acid substitution in the matrix protein (M51R) of Vesicular Stomatitis New Jersey virus impairs replication in cultured porcine macrophages and results in significant attenuation in pigs. Frontiers in Microbiology. https://doi.org/10.3389/ fmicb.2020.01123.
- Palinski, R.M., Pauszek, S., Burruss, D.N., Savory, H., Humphreys, J., Pelzel-McCluskey, A.M., Arzt, J., Peters, D., Rodriguez, L.L. 2020. Whole- genome sequences of Vesicular Stomatitis Virus isolates from the 2004-2006 U.S. outbreaks reveal evidence of molecular adaptation to ecological variables. Molecular Ecology. https://doi.org/10.3390/ proceedings2020050076.
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