EFFICACY OF A PAN-GENOTYPIC INFLUENZA A VIRUS ANTISENSE LOCKED NUCLEIC ACID OLIGONUCLEOTIDE (LNA) AS A PREVENTATIVE AND THERAPEUTIC IN SWINE

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: ACTIVE
• Funding Source: USDA INHOUSE
• Reporting Frequency: Annual
• Accession No.: 0441215
• Project Start Date: Jul 11, 2022
• Project End Date: Jan 1, 2025
• Program Code: [(N/A)]- (N/A)

Recipient Organization
AGRICULTURAL RESEARCH SERVICE
(N/A)
AMES,IA 50010

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

20%

Research Effort Categories

Basic

60%

Applied

20%

Developmental

20%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
311	3510	1040	100%

Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3510 - Swine, live animal;

Field Of Science
1040 - Molecular biology;

Keywords

influenza

a

virus

locked

nucleic

acide

oligonucleotide

swine

respiratory

disease

Goals / Objectives
Determine the efficacy of a pan-genotypic influenza A virus antisense locked nucleic acid oligonucleotide (LNA) as a preventative and therapeutic in swine.

Project Methods
Two challenge studies with ~57 pigs per study will be conducted in which an LNA will be administered to swine either daily for three days before exposure (Study A; prevention) or daily starting a day after exposure (Study B; therapeutic) to influenza A virus. The following will be evaluated to determine the effectiveness of the LNA: Virus shedding via virus isolation/titration/PCR, gross lesions and histologic lesions.