16S METAGENOMIC SEQUENCING ANALYSIS

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: ACTIVE
• Funding Source: USDA COOPERATIVE AGREEMENT
• Reporting Frequency: Annual
• Accession No.: 0439633
• Project Start Date: Jul 12, 2021
• Project End Date: Jul 11, 2023
• Program Code: [(N/A)]- (N/A)

Recipient Organization
UNIV OF WISCONSIN
21 N PARK ST STE 6401
MADISON,WI 53715-1218

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

20%

Research Effort Categories

Basic

50%

Applied

20%

Developmental

30%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
311	3310	1100	20%
311	3410	1100	65%
311	3510	1100	15%

Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3310 - Beef cattle, live animal; 3510 - Swine, live animal; 3410 - Dairy cattle, live animal;

Field Of Science
1100 - Bacteriology;

Keywords

cattle

disease

digital

dermatitis

metagenomics

bacteria

sequencing

data

analysis

Goals / Objectives
Analysis of bacterial 16S metagenome sequences of samples collected from experimental digital dermatitis lesions in sheep model collected at different timepoints and analyze the data for similarities/differences across timepoints in relation to bacterial invasion and skin pathology.

Project Methods
Digital dermatitis lesions were experimentally induced in sheep model and samples collected at specific timepoints post inoculation. DNA was extracted and librabries were prepared and sequenced. Sequences will be analyzed by group (mock or infected) by timepoint, looking for correlation between bacterial community development, bacterial invasion in the tissues and pathological responses observed. This data will contribute to knowledge of the underlying pathogenesis of digital dermatitis lesions.