TEAM MAIZE HOPPER

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: ACTIVE
• Funding Source: USDA INHOUSE
• Reporting Frequency: Annual
• Accession No.: 0433618
• Project Start Date: Jul 1, 2017
• Project End Date: Sep 30, 2021
• Program Code: [(N/A)]- (N/A)

Recipient Organization
AGRICULTURAL RESEARCH SERVICE
(N/A)
WOOSTER,OH 44691

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

30%

Research Effort Categories

Basic

70%

Applied

30%

Developmental

0%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
212	1510	1040	68%
212	1820	1160	32%

Knowledge Area
212 - Pathogens and Nematodes Affecting Plants;

Subject Of Investigation
1510 - Corn; 1820 - Soybean;

Field Of Science
1040 - Molecular biology; 1160 - Pathology;

Keywords

maize

maize

mosaic

virus

maize

fine

streak

virus

perigrinus

maidis

graminella

nigrifrons

Goals / Objectives
Develop genome editing systems for maize based expression of CRISPR-Cas from RNA virus vectors.

Project Methods
We will develop maize infecting rhabdovirus-based virus vectors to deliver Cas nucleases and guide RNAs to maize using the virus¿ planthopper and leafhopper vectors. We will develop infectious rhabdovirus clones and will use these to stably express green fluorescent protein in maize. Subsequently, we will develop protocols for insect transmission of modified viruses expressing Cas9 with guide RNAs and other phenotype altering proteins to maize. We will assess the capability of the system in large-scale greenhouse experiments that will also evaluate the potential for viruses and expressed genes to move into non-target populations.