Source: ARIZONA STATE UNIVERSITY submitted to NRP
SPECIES IDENTIFICATION CONFIRMATION OF MEDICAGO OF NPGS GERMPLASM
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
ACTIVE
Funding Source
USDA COOPERATIVE AGREEMENT
Reporting Frequency
Annual
Accession No.
0431268
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jun 30, 2016
Project End Date
Jun 30, 2018
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
ARIZONA STATE UNIVERSITY
660 S MILL AVE STE 312
TEMPE,AZ 85281-3670
Performing Department
APPLIED SCIENCES
Non Technical Summary
(N/A)
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2021410108010%
2021412108010%
2021414108010%
2021419108040%
2021621108010%
2022010108010%
2022410108010%
Knowledge Area
202 - Plant Genetic Resources;

Subject Of Investigation
1419 - Leguminous vegetables, general/other; 2010 - Sugar beet; 1410 - Beans (dry); 1414 - Lentil; 1621 - Cool season perennial grasses; 1412 - Peas (dry); 2410 - Cross-commodity research--multiple crops;

Field Of Science
1080 - Genetics;
Goals / Objectives
Confirm identification of USDA accessions of species in the genus Medicago section that includes secondary and tertiary wild relatives of alfalfa using DNA barcoding and ploidy level. Obtain relevant information about genetic diversity among accessions of crop wild relatives of alfalfa. Confirm identification of selected USDA accessions of species most closely related to M. truncatula and other species in a revised subsection Pachyspireae using DNA barcode sequence data. Provide a rough estimate of the proportion of accessions that might be misidentified within groups sampled. GRIN-Global: Add information for sampled accessions of Medicago. Contribute to student education and training with the goal of increasing understanding of crop wild relatives, molecular biology techniques and importance of study of crop wild relatives in agriculture.
Project Methods
Project attempts to delineate and potentially identify taxonomically mislabeled accessions within the temperate forage legume germplasm accessions focusing on species within the Medicago genus. This will be accomplished by screening a representative sample of accessions for their ploidy by using flow cytometry and by examining nuclear and organellar DNA sequences (¿DNA barcoding¿). Ploidy and sequence information will be compared to known true-to-type taxonomic samples and sequence information in NCBI¿s GenBank. Phylogenetic analysis will use standard software tools for sequence comparisons. Information generated will be analyzed and information on any potential mislabeling will be sent to the ARS Principal Investigator for correction. Accession-associated data generated will also be incorporated into the USDA-ARS National Plant Germplasm System¿s GRIN-Global database, into NCBI¿s GenBank databses as well as assembled into a peer-reviewed publication.