Recipient Organization
NORTH DAKOTA STATE UNIV
1310 BOLLEY DR
FARGO,ND 58105-5750
Performing Department
PLANT SCIENCE
Non Technical Summary
(N/A)
Animal Health Component
40%
Research Effort Categories
Basic
60%
Applied
40%
Developmental
0%
Goals / Objectives
To develop efficient protocols for doubled haploid production in sunflower that can be adopted by seed companies with modest laboratory facilities and personnel. The technique we will develop will allow for paternal haploid production, which would allow any inbred or DH pollen source to be converted to a cytoplasmic male sterile (CMS) isoline with a single cross. It could also allow for DH population development from F1 or F2 populations.
Project Methods
Our approach will consist of three phases: (1) Inducer event discovery in a sunflower mutagenesis panel, (2) Isolation of the responsible gene(s) and transfer to a CMS background, and (3) Purify and release inducer stocks with both H. petiolaris [French, otherwise known as PET1], and H. annuus cytoplasms. Discovery of the inducer event should take place in the first two years of the project. A dual visible marker system will allow for easy visible selection of haploid and doubled haploid plants from our testcrosses. Cytological or marker validation will take place to further confirm visible marker findings. Isolation of the genetics will commence the same season as the final discovery step and will involve additional, focused progeny testing from the original mutagenesis line of interest. With the ever reducing costs of whole genome shotgun sequencing and a fairly robust sunflower sequence completed, it is likely that we will leverage the known native genetic background of the parents to find lesions in silico, identify molecular markers, and enhance introgression into both CMS and non-CMS cytoplasms. The genetic stocks will then be purified, increased, and released to the program participants, complete with any visible marker required to quickly identify haploid and autonomously doubled haploid progeny in any breeding program.