Progress 11/12/14 to 03/05/15
Outputs
Progress Report Objectives (from AD-416): The long-term objective of this project is to characterize the pathogenesis of fish diseases and to develop approaches to control diseases in warm water fishes. Over the next 5 years, our project at the Stuttgart National Aquaculture Research Center (SNARC) will focus on the following objectives: Objective 1. Characterize genetic variation of white bass and sunshine bass resistance to columnaris and develop strategies to control columnaris in warm water aquaculture. Subobjective 1A. Develop a Flavobacterium columnare disease model in white bass (Morone chrysops) and sunshine bass (female Morone chrysops x male M. saxitalis). Determine whether there is genetic variation for survival of white bass and sunshine bass in response to columnaris infection (Darwish and Mitchell). Subobjective 1B. Characterize Flavobacterium columnare virulence genes using realtime PCR to evaluate gene expression (Straus). Objective 2. Evaluate compounds and methods to treat bacterial and fungal (columnaris, streptococcosis, and saprolegniasis) fish diseases. Subobjective 2A. Control columnaris in channel catfish with copper sulfate (Darwish, Mitchell, and Straus). Subobjective 2B. Control saprolegniasis on channel catfish eggs and improving hatch rate by chemical means (Mitchell and Straus). Subobjective 2C. Evaluate caprylic acid to control streptococcosis in sunshine bass (Darwish). These objectives are designed to address current research concerns of stakeholders and will help in the ultimate goal of better treatments for disease epizootics resulting in higher profits for producers. Approach (from AD-416): Objective 1. An infection method will be developed for white bass and sunshine bass to produce consistent mortalities similar to a natural infection of the bacterial disease columnaris caused by F. columnare. Resistance to columnaris will be characterized in families of sunshine bass and white bass, and maternal and paternal effects will be determined. The expression level of the putative virulence genes(identified by sequence comparison) in F. columnare will be characterized to provide insight into bacterial pathogenesis. Reverse-transcriptase real-time PCR methods will be developed and used to profile the transcript level of selected genes in isolates of high and low pathogenicity. Objective 2. The efficacy of CuSO4 against columnaris will be tested in vitro using micro-dilution assays and in vivo by using four treatment levels of CuSO4 against a columnaris disease model simulating a natural infection. The scenarios used to expose channel catfish to CuSO4 prior to bacterial challenge based on previous work will consist of either pre- exposure to CuSO4 dissolved in the water or pre-exposure to CuSO4 incorporated in feed. Research will be designed to improve the effectiveness of CuSO4, H2O2, and formalin to fungi on channel catfish eggs and on the hatch rate of these eggs. A new aquaculture therapeutant (peracetic acid) will be used to treat saprolegniasis on intact channel catfish eggs in hatching troughs. The concentration of caprylic acid that inhibits the bacteria S. iniae will be determined in vitro and its potential efficacy will be assessed against an experimental S. iniae infection in sunshine bass. This 113-day project served as a bridge between the previous project (6225-32000-005-00D, Strategies for Fish Disease Control and Prevention) and the newly approved project (6028-32000-007-00D, The Role of Mucosal Surfaces and Microflora in Immunity and Disease Prevention). Channel catfish and blue catfish brood fish were selected and conditioned for spawning in the spring. Hatchery facilities were cleaned and prepared for spawning. Scientists bath immersed Stuttgart channel catfish with an attenuated strain of Flavobacterium columnare. Scientists later challenged these and control animals with wild type F. columnare in a �low-flow� disease challenge system. Animals that survived the disease challenge from both groups were maintained for future studies that will examine the adaptive immune response to F. columnare. Continued research examining the utility and feasibility of peracetic acid as a disinfectant in aquaculture settings. Research focused on characterizing the toxicity of peracetic acid to various commercially relevant species of freshwater fish.
Impacts
(N/A)
Publications
|
|