DEVELOPMENT OF DIAGNOSTIC RESOURCES TO SUPPORT FRUIT FLY EXCLUSION AND ERADICATION - PHASE 3

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: ACTIVE
• Funding Source: USDA COOPERATIVE AGREEMENT
• Reporting Frequency: Annual
• Accession No.: 0426984
• Project Start Date: Sep 17, 2014
• Project End Date: Aug 31, 2016
• Program Code: [(N/A)]- (N/A)

Recipient Organization
UNIV OF HAWAII
3190 MAILE WAY
HONOLULU,HI 96822

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

50%

Research Effort Categories

Basic

40%

Applied

50%

Developmental

10%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
211	1099	1130	100%

Knowledge Area
211 - Insects, Mites, and Other Arthropods Affecting Plants;

Subject Of Investigation
1099 - Tropical/subtropical fruit, general/other;

Field Of Science
1130 - Entomology and acarology;

Keywords

tephritidae

mediterranean

fruit

fly

oriental

fruit

fly

genomic

markers

Goals / Objectives
1. Complete multi-locus phylogenetic analysis of Bactrocera collections and examine character evolution (e.g., morphological variants, geographic variants, and chemical ecology/trapping differences) to test hypotheses regarding species limits, and to improve trapping, control, and quarantine. 2. Use targeted amplicon high throughput sequencing to evaluate ITS locus as diagnostic marker for fruit flies in the genera Anastrepha, Bactrocera, and Ceratitis. 3. Complete the analysis of SNP markers for Bactrocera species-level and population-level analyses. 4. Develop a high throughput procedure for genotyping the Oriental fruit fly and Medfly. 5. Complete technology transfer for Vienna 7 and Vienna 8 diagnostic markers to enable identification of TSL-linked DNA in captured medflies.

Project Methods
Generate fruit fly specimen inventory, characterize the inventory using molecular and morphological techniques, and develop diagnostic tools based on these characters.